Uptake of triiodothyronine and triiodothyroacetic acid in neonatal rat cardiomyocytes: effects of metabolites and analogs

Cellular and nuclear uptake of [125I]tri-iodothyronine (T3) and [125I]triiodothyroacetic acid (Triac) were compared in cardiomyocytes of 2-3 day old rats, and the effect of thyroid hormone analogs on cellular T(3) uptake was measured. Cells (5-10 x 10(5) per well) were cultured in DMEM-M199 with 5% horse serum and 5% FCS. Incubations were performed for from 15 min to 24 h at 37 degrees C in the same medium, 0.5% BSA and [125I]T3 (100 pM), or [125I]Triac (240 pM). Expressed as % dose, T(3) uptake was five times Triac uptake, but expressed as fmol/pM free hormone, Triac uptake was at least 30% (P<0.001) greater than T3 uptake, whereas the relative nuclear binding of the two tracers was comparable. The 15 min uptake of [125I]T3 was competitively inhibited by 10 microM unlabeled T3 (45-52%; P<0.001) or 3,3'- diiodothyronine (T2) (52%; P<0.001), and to a smaller extent by thyroxine (T(4)) (27%; 0.05<P<0.1). In contrast, 10 microM 3,5-T2, Triac, or tetraiodothyroacetic acid (Tetrac) did not affect T3 uptake after 15 min or after 24 h. Diiodothyropropionic acid (DITPA) (10 microM) reduced 15-min T3 uptake by about 24% (P<0.05), but it had a greater effect after 4 h (56%; P<0.001). Exposure to 10 nM DITPA during culture reduced cellular T3 uptake, as did 10 nM T3, suggesting down-regulation of the plasma membrane T3 transporters. We conclude that i) Triac is taken up by cardiomyocytes; ii) 3,3'-T2 and, to a lesser extent, DITPA and T4 interfere with plasma membrane transport of T3, whereas 3,5-T2, Triac, or Tetrac do not; iii) the transport mechanism for Triac is probably different from that for T3

AutoML adoption in ML software

Algorithms and the Foundations of Software technolog

Using semantic web technology to accelerate plant breeding

One goal within plant breeding is to find the causal gene(s) explaining a given phenotype. Semantic web technology brings opportu- nities to integration data and information accross spread data sources. Chebi2gene and Marker2sequence are two applications relying on this se- mantic web technology to integration genes, proteins, metabolites, path- ways, literature. Their web-based interface allows biologists to use and explore this network of information

Economic and environmental analysis of energy efficiency measures in agriculture, Case Studies and trade offs

This report illustrates case studies with an in-depth analysis of the interactions of energy efficiency measures with farm economics and the environmental impact (GHG) of the measures across Europe. The analyses followed a common methodology considering the farm gate as the system boundary. Therefore, considerable energy use in the post-processing of agricultural products were only taken into account, when they can be assumed to be realized on the farm. The analyses of the energy use, economic and environmental effects follow an LCA approach taking into account all costs of the production, including those for machines according to the concept of “useful life” of the machines used. The environmental effects of energy saving were illustrated with the greenhouse gas emission effect of the energy efficiency measures. The case studies are only a selection of specific energy saving measures across Europe and therefore cannot be regarded as representative for all Europe. Nevertheless, they will help to understand constraints and opportunities for increased energy efficiency in agriculture, which can be used to translate to an agenda of practical action or applied research. The findings are valid sometimes only in the specific regional settings, sometimes they are of general validity

On Traversable Lorentzian Wormholes in the Vacuum Low Energy Effective String Theory in Einstein and Jordan Frames

Three new classes (II-IV) of solutions of the vacuum low energy effective string theory in four dimensions are derived. Wormhole solutions are investigated in those solutions including the class I case both in the Einstein and in the Jordan (string) frame. It turns out that, of the eight classes of solutions investigated (four in the Einstein frame and four in the corresponding string frame), massive Lorentzian traversable wormholes exist in five classes. Nontrivial massless limit exists only in class I Einstein frame solution while none at all exists in the string frame. An investigation of test scalar charge motion in the class I solution in the two frames is carried out by using the Plebanski-Sawicki theorem. A curious consequence is that the motion around the extremal zero (Keplerian) mass configuration leads, as a result of scalar-scalar interaction, to a new hypothetical "mass" that confines test scalar charges in bound orbits, but does not interact with neutral test particles.Comment: 18 page

Gadoxetic acid-enhanced magnetic resonance imaging significantly influences the clinical course in patients with colorectal liver metastases

Surgical oncolog