Making citizen science count: best practices and challenges of citizen science projects on plastics in aquatic environments

There is considerable scientific and societal concern about plastic pollution, which has resulted in citizen science projects to study the scale of the issue. Citizen science is a cost-effective way to gather data over a large geographical range while simultaneously raising public awareness on the problem. Because the experiences of researchers involved in these projects are not yet adequately covered, this paper presents the findings from ten semi-structured qualitative interviews with researchers leading a citizen science project on micro- or macroplastics. Our results show it is important to specify the goal(s) of the project and that expertise on communication and data science is needed. Furthermore, simple protocols, quality control, and engagement with volunteers and the public are key elements for successful projects. From these results, a framework with recommendations was drafted, which can be used by anyone who wants to develop or improve citizen science projects.Science Communication and Societ

Polystyrene nanoplastics disrupt glucose metabolism and cortisol levels with a possible link to behavioural changes in larval zebrafish

Plastic nanoparticles originating from weathering plastic waste are emerging contaminants in aquatic environments, with unknown modes of action in aquatic organisms. Recent studies suggest that internalised nanoplastics may disrupt processes related to energy metabolism. Such disruption can be crucial for organisms during development and may ultimately lead to changes in behaviour. Here, we investigated the link between polystyrene nanoplastic (PSNP)-induced signalling events and behavioural changes. Larval zebrafish exhibited PSNP accumulation in the pancreas, which coincided with a decreased glucose level. By using hyperglycemic and glucocorticoid receptor (Gr) mutant larvae, we demonstrate that the PSNP-induced disruption in glucose homoeostasis coincided with increased cortisol secretion and hyperactivity in challenge phases. Our work sheds new light on a potential mechanism underlying nanoplastics toxicity in fish, suggesting that the adverse effect of PSNPs are at least in part mediated by Gr activation in response to disrupted glucose homeostasis, ultimately leading to aberrant locomotor activity

Multiple verification in computational modeling of bone pathologies

We introduce a model checking approach to diagnose the emerging of bone pathologies. The implementation of a new model of bone remodeling in PRISM has led to an interesting characterization of osteoporosis as a defective bone remodeling dynamics with respect to other bone pathologies. Our approach allows to derive three types of model checking-based diagnostic estimators. The first diagnostic measure focuses on the level of bone mineral density, which is currently used in medical practice. In addition, we have introduced a novel diagnostic estimator which uses the full patient clinical record, here simulated using the modeling framework. This estimator detects rapid (months) negative changes in bone mineral density. Independently of the actual bone mineral density, when the decrease occurs rapidly it is important to alarm the patient and monitor him/her more closely to detect insurgence of other bone co-morbidities. A third estimator takes into account the variance of the bone density, which could address the investigation of metabolic syndromes, diabetes and cancer. Our implementation could make use of different logical combinations of these statistical estimators and could incorporate other biomarkers for other systemic co-morbidities (for example diabetes and thalassemia). We are delighted to report that the combination of stochastic modeling with formal methods motivate new diagnostic framework for complex pathologies. In particular our approach takes into consideration important properties of biosystems such as multiscale and self-adaptiveness. The multi-diagnosis could be further expanded, inching towards the complexity of human diseases. Finally, we briefly introduce self-adaptiveness in formal methods which is a key property in the regulative mechanisms of biological systems and well known in other mathematical and engineering areas.Comment: In Proceedings CompMod 2011, arXiv:1109.104

NK1 antagonists attenuate tau phosphorylation after blast and repeated concussive injury

Exposure to repeated concussive traumatic brain injury (TBI) and to blast-induced TBI has been associated with the potential development of the neurodegenerative condition known as chronic traumatic encephalopathy (CTE). CTE is characterized by the accumulation of hyperphosphorylated tau protein, with the resultant tau tangles thought to initiate the cognitive and behavioral manifestations that appear as the condition progresses. However, the mechanisms linking concussive and blast TBI with tau hyperphosphorylation are unknown. Here we show that single moderate TBI, repeated concussive TBI and blast-induced mild TBI all result in hyperphosphorylation of tau via a substance P mediated mechanism. Post-injury administration of a substance P, NK1 receptor antagonist attenuated the injury-induced phosphorylation of tau by modulating the activity of several key kinases including Akt, ERK1/2 and JNK, and was associated with improvement in neurological outcome. We also demonstrate that inhibition of the TRPV1 mechanoreceptor, which is linked to substance P release, attenuated injury-associated tau hyperphosphorylation, but only when it was administered prior to injury. Our results demonstrate that TBI-mediated stimulation of brain mechanoreceptors is associated with substance P release and consequent tau hyperphosphorylation, with administration of an NK1 receptor antagonist attenuating tau phosphorylation and associated neurological deficits. NK1 antagonists may thus represent a pharmacological approach to attenuate the potential development of CTE following concussive and blast TBI.Frances Corrigan, Ibolja Cernak, Kelly McAteer, Sarah C. Hellewell, JefreyV. Rosenfeld ... et al

Direct observation by resonant tunneling of the B^+ level in a delta-doped silicon barrier

We observe a resonance in the conductance of silicon tunneling devices with a delta-doped barrier. The position of the resonance indicates that it arises from tunneling through the B^+ state of the boron atoms of the delta-layer. Since the emitter Fermi level in our devices is a field-independent reference energy, we are able to directly observe the diamagnetic shift of the B^+ level. This is contrary to the situation in magneto-optical spectroscopy, where the shift is absorbed in the measured ionization energy.Comment: submitted to PR

Physics of rotation in stellar models

In these lecture notes, we present the equations presently used in stellar interior models in order to compute the effects of axial rotation. We discuss the hypotheses made. We suggest that the effects of rotation might play a key role at low metallicity.Comment: 32 pages, 7 figures, lectures, CNRS school, will be published by Springe

Magnetism in Herbig Ae/Be stars and the link to the Ap/Bp stars

Among the A/B stars, about 5% host large-scale organised magnetic fields. These magnetic stars show also abundance anomalies in their spectra, and are therefore called the magnetic Ap/Bp stars. Most of these stars are also slow rotators compared to the normal A and B stars. Today, one of the greatest challenges concerning the Ap/Bp stars is to understand the origin of their slow rotation and their magnetic fields. The favoured hypothesis for the latter is that the fields are fosils, which implies that the magnetic fields subsist throughout the different evolutionary phases, and in particular during the pre-main sequence phase. The existence of magnetic fields at the pre-main sequence phase is also required to explain the slow rotation of Ap/Bp stars. During the last 3 years we performed a spectropolarimetric survey of the Herbig Ae/Be stars in the field and in young clusters, in order to investigate their magnetism and rotation. These investigations have resulted in the detection and/or confirmation of magnetic fields in 8 Herbig Ae/Be stars, ranging in mass from 2 to nearly 15 solar masses. In this paper I will present the results of our survey, as well as their implications for the origin and evolution of the magnetic fields and rotation of the A and B stars.Comment: To appear in the proceedings of "Ecole d'astronomie du CNRS et du PNPS : Les champs magnetiques stellaires", editors: C. Neiner and J.-P. Zahn, EAS Publications Serie

Horizontal Branch Stars: The Interplay between Observations and Theory, and Insights into the Formation of the Galaxy

We review HB stars in a broad astrophysical context, including both variable and non-variable stars. A reassessment of the Oosterhoff dichotomy is presented, which provides unprecedented detail regarding its origin and systematics. We show that the Oosterhoff dichotomy and the distribution of globular clusters (GCs) in the HB morphology-metallicity plane both exclude, with high statistical significance, the possibility that the Galactic halo may have formed from the accretion of dwarf galaxies resembling present-day Milky Way satellites such as Fornax, Sagittarius, and the LMC. A rediscussion of the second-parameter problem is presented. A technique is proposed to estimate the HB types of extragalactic GCs on the basis of integrated far-UV photometry. The relationship between the absolute V magnitude of the HB at the RR Lyrae level and metallicity, as obtained on the basis of trigonometric parallax measurements for the star RR Lyrae, is also revisited, giving a distance modulus to the LMC of (m-M)_0 = 18.44+/-0.11. RR Lyrae period change rates are studied. Finally, the conductive opacities used in evolutionary calculations of low-mass stars are investigated. [ABRIDGED]Comment: 56 pages, 22 figures. Invited review, to appear in Astrophysics and Space Scienc