11 research outputs found
Mathematical model for fragmentation of bacterial inclusion bodies
Get PDFBacterial inclusion bodies are microscopic, ovoid-shaped aggregates of insoluble protein. Under protease exposure a digestion process is produced that reveals a variable fragmentation rate, not compatible with a surface restricted erosion of body particles, or an uniform sensibility to the fragmentation agent. The modelling and fitting of experimental data is performed in two steps. (a) Due to poor estimation of protein amounts only first derivatives can be numerically evaluated, and a non-linear first-order fragmentation model is adopted. Although it is a very good approximation for intermediate points, the asymtotic behaviour of the solution is inconsistent with the fragmentation process. (b) The solution of previous kinetic modelling is used to compute higher-order derivatives in intermediate points and to adopt a higher-order lineal model for the overall interval with protein fragmentation. The resulting model consists in a superposition of Poisson processes associated with several populations of protein with different fragmentation resistance. Numerical estimation of model constants is also described and discussed. In particular, an iterative method of weighted least squares is used in order to obtain minimum variance parameters.Postprint (published version
Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor)
Full text linkMathematical model for fragmentation of bacterial inclusion bodies
No full textBacterial inclusion bodies are microscopic, ovoid-shaped aggregates of insoluble protein. Under protease exposure a digestion process is produced that reveals a variable fragmentation rate, not compatible with a surface restricted erosion of body particles, or an uniform sensibility to the fragmentation agent. The modelling and fitting of experimental data is performed in two steps. (a) Due to poor estimation of protein amounts only first derivatives can be numerically evaluated, and a non-linear first-order fragmentation model is adopted. Although it is a very good approximation for intermediate points, the asymtotic behaviour of the solution is inconsistent with the fragmentation process. (b) The solution of previous kinetic modelling is used to compute higher-order derivatives in intermediate points and to adopt a higher-order lineal model for the overall interval with protein fragmentation. The resulting model consists in a superposition of Poisson processes associated with several populations of protein with different fragmentation resistance. Numerical estimation of model constants is also described and discussed. In particular, an iterative method of weighted least squares is used in order to obtain minimum variance parameters
Métodos anticonceptivos utilizados por población inmigrante y condicionantes que influyen en su elección en un área sanitaria de Zaragoza
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Impact of Insect Densities Tribolium Castaneum on the Benzoquinone Secretions and Aflatoxins Levels in Wheat Flour During Storage Periods
No full textA pilot field-scale study on biotrickling filter treatment of NH3-containing odorous gases from organic waste composting plants
No full textElimination of the Effect of Some Herbicides on the Growth of Zea mays and Accumulation in the Soil Using Urea
No full textDirectrices del grupo español de psoriasis (GEP) basadas en la evidencia para el uso de medicamentos biológicos en pacientes con psoriasis en localizaciones de difícil tratamiento (uñas, cuero cabelludo, palmas y plantas)
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