In Silico analysis of Gastric carcinoma Serial Analysis of Gene Expression libraries reveals different profiles associated with ethnicity

Worldwide gastric carcinoma has marked geographical variations and worse outcome in patients from the West compared to the East. Although these differences has been explained by better diagnostic criteria, improved staging methods and more radical surgery, emerging evidence supports the concept that gene expression differences associated to ethnicity might contribute to this disparate outcome. Here, we collected datasets from 4 normal and 11 gastric carcinoma Serial Gene Expression Analysis (SAGE) libraries from two different ethnicities. All normal SAGE libraries as well as 7 tumor libraries were from the West and 4 tumor libraries were from the East. These datasets we compare by Correspondence Analysis and Support Tree analysis and specific differences in tags expression were identified by Significance Analysis for Microarray. Tags to gene assignments were performed by CGAP-SAGE Genie or TAGmapper. The analysis of global transcriptome shows a clear separation between normal and tumor libraries with 90 tags differentially expressed. A clear separation was also found between the West and the East tumor libraries with 54 tags differentially expressed. Tags to gene assignments identified 15 genes, 5 of them with significant higher expression in the West libraries in comparison to the East libraries. qRT-PCR in cell lines from west and east origin confirmed these differences. Interestingly, two of these genes have been associated to aggressiveness (COL1A1 and KLK10). In conclusion we found that in silico analysis of SAGE libraries from two different ethnicities reveal differences in gene expression profile. These expression differences might contribute to explain the disparate outcome between the West and the East

Envejecimiento de la población

•Actividades básicas de la vida diaria en personas mayores y factores asociados •Asociación entre depresión y posesión de mascotas en personas mayores •Calidad de vida en adultos mayores de Santiago aplicando el instrumento WHOQOL-BREF •Calidad de vida en usuarios con enfermedad de Parkinson, demencia y sus cuidadores, comuna de Vitacura •Caracterización de egresos hospitalarios de adultos mayores en Puerto Natales (2007-2009) •Comportamiento de las patologías incluidas como GES para el adulto mayor atendido en un Cesfam •Contribución de vitaminas y minerales a las ingestas recomendadas diarias en ancianos institucionalizados de Madrid •Estado de salud oral del paciente inscrito en el Programa de Visita Domiciliaria •Evaluación del programa de discapacidad severa en Casablanca con la matriz de marco lógico •Factores asociados a satisfacción vital en una cohorte de adultos mayores de Santiago, Chile •Pauta instrumental para la identificación de riesgos para el adulto mayor autovalente, en su vivienda •Perfil farmacológico del paciente geriátrico institucionalizado y posibles consecuencias en el deterioro cognitivo •Programa de cuidados paliativos y alivio del dolor en Puerto Natales •Rehabilitación mandibular implantoprotésica: efecto en calidad de vida relacionada con salud bucal en adultos mayores •Salud bucodental en adultos mayores autovalentes de la Región de Valparaíso •Transición epidemiológica y el estudio de carga de enfermedad en Brasi

Implementación de la técnica de biología molecular PCR múltiple para la detección de mycoplasma pneumoniae y chlamydia pneumoniae como método de apoyo diagnóstico en pacientes pediátricos con infecciones respiratorias agudas

Tesis (Tecnólogo Médico)Las Infecciones Respiratorias Agudas (IRA) representan un problema de gran relevancia epidemiológica en los niños, siendo el principal motivo de consulta pediátrica en la atención primaria y en servicios de urgencia. Además, constituye la tercera causa de mortalidad infantil en nuestro país. Con respecto a su etiología, los virus son la causa más frecuente seguido por bacterias tales como Streptococcus pneumoniae, Haemophilus influenzae, Streptococcus pyogenes entre otros. Existe otro grupo de bacterias conocidas como atípicas tales como: Mycoplasma pneumoniae, Chlamydia pneumoniae y Legionella pneumophila que también causan IRA, pero con menor frecuencia. Estudios epidemiológicos recientes muestran que estos patógenos atípicos han aumentado su incidencia en enfermedades respiratorias agudas, dificultando el manejo clínico de los pacientes debido a que se entrecruzan signos y síntomas con las infecciones virales, obligando frecuentemente al médico a utilizar antimicrobianos de forma innecesaria. Por ello, la detección oportuna de M. pneumoniae y C. pneumoniae, permite confirmar el diagnóstico clínico y ofrecer un tratamiento específico y eficaz para el paciente, adicionalmente permite dar a conocer al clínico su impacto epidemiológico

Reprimo as a potential biomarker for early detection in gastric cancer

Purpose: Gastric cancer is a curable disease if diagnosed at early stage. However, most cases are diagnosed at advanced stage because of the lack of screening programs. Therefore, the identification of plasma biomarkers for early detection is necessary. Experimental Design: To search for these biomarkers, we evaluated the DNA methylation patterns of 24 genes by Methylation-specific PCR in primary tissues from 32 retrospectively collected gastric cancer cases (testing group). Correlation between methylation and gene expression was evaluated in the MKN-45 cell line after treatment with 5-aza-2′- deoxycytidine. The most frequently hypermethylated genes were next evaluated in primary tissues and plasma samples from 43 prospectively collected gastric cancer cases as well as plasma samples from 31 asymptomatic age- and gender-matched controls (validation group). Results: In the testing group, 11 genes were hypermethylated in at least 50% of cases (APC, SHP1, E-cadherin, ER, Reprimo, SEMA3B

Correspondence Analysis of normal and tumor SAGE libraries of the stomach

A two-dimensional plot is shown where the green dots represent all the normal libraries, the blue dots are the East tumor libraries, and the red, orange and yellow dots are West tumor libraries, microdissected, xenograft and bulk respectively.<p><b>Copyright information:</b></p><p>Taken from "In Silico analysis of Gastric carcinoma Serial Analysis of Gene Expression libraries reveals different profiles associated with ethnicity"</p><p>http://www.molecular-cancer.com/content/7/1/22</p><p>Molecular Cancer 2008;7():22-22.</p><p>Published online 27 Feb 2008</p><p>PMCID:PMC2323622.</p><p></p

Serial Analysis for Microarray of East and West gastric carcinoma SAGE libraries

To the left and shown in orange color, the significant tags with higher expression in the West tumor libraries; to the right and shown in blue color, the significant tags with higher expression in the East tumor libraries.<p><b>Copyright information:</b></p><p>Taken from "In Silico analysis of Gastric carcinoma Serial Analysis of Gene Expression libraries reveals different profiles associated with ethnicity"</p><p>http://www.molecular-cancer.com/content/7/1/22</p><p>Molecular Cancer 2008;7():22-22.</p><p>Published online 27 Feb 2008</p><p>PMCID:PMC2323622.</p><p></p

Efficacy of stem cell secretome loaded in hyaluronate sponge for topical treatment of psoriasis

Abstract Psoriasis vulgaris is an inflammatory disease characterized by distinctive skin lesions and dysregulated angiogenesis. Recent research uses stem cell secretion products (CM); a set of bioactive factors with therapeutic properties that regulate several cellular processes, including tissue repair and angiogenesis. The aim of this work was to evaluate the effect of CM of Wharton's gelatin MSC (hWJCM) in a treatment based on the bioactivation of a hyaluronic acid matrix (HA hWJCM) in a psoriasiform‐like dermatitis (PD) mouse model. A preclinical study was conducted on PD mice. The effect of hWJCM, Clobetasol (Clob) gold standard, HA Ctrl, and HA hWJCM was tested topically evaluating severity of PD, mice weight as well as skin, liver, and spleen appearance. Treatment with either hWJCM, HA Ctrl or HA hWJCM, resulted in significant improvement of the PD phenotype. Moreover, treatment with HA hWJCM reduced the Psoriasis Area Severity Index (PASI), aberrant angiogenesis, and discomfort associated with the disease, leading to total recovery of body weight. We suggest that the topical application of HA hWJCM can be an effective noninvasive therapeutic solution for psoriasis, in addition to other skin diseases, laying the groundwork for future studies in human patients

Globalización y salud pública

Evoluci&oacute;n espacio-temporal de la pandemia AH1N1 en establecimientos de educaci&oacute;n, Regi&oacute;n MetropolitanaTeledermatolog&iacute;a en atenci&oacute;n primaria: &iquest;aplicable a nuestra realidad?Uso de las TIC y redes sociales en atenci&oacute;n primaria de salu

Kinetic Characterization and Allosteric Inhibition of the <i>Yersinia pestis</i> 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase (MEP Synthase)

<div><p>The methylerythritol phosphate (MEP) pathway found in many bacteria governs the synthesis of isoprenoids, which are crucial lipid precursors for vital cell components such as ubiquinone. Because mammals synthesize isoprenoids via an alternate pathway, the bacterial MEP pathway is an attractive target for novel antibiotic development, necessitated by emerging antibiotic resistance as well as biodefense concerns. The first committed step in the MEP pathway is the reduction and isomerization of 1-deoxy-D-xylulose-5-phosphate (DXP) to methylerythritol phosphate (MEP), catalyzed by MEP synthase. To facilitate drug development, we cloned, expressed, purified, and characterized MEP synthase from <i>Yersinia pestis</i>. Enzyme assays indicate apparent kinetic constants of K_M^DXP = 252 µM and K_M^NADPH = 13 µM, IC₅₀ values for fosmidomycin and FR900098 of 710 nM and 231 nM respectively, and K_i values for fosmidomycin and FR900098 of 251 nM and 101 nM respectively. To ascertain if the <i>Y. pestis</i> MEP synthase was amenable to a high-throughput screening campaign, the Z-factor was determined (0.9) then the purified enzyme was screened against a pilot scale library containing rationally designed fosmidomycin analogs and natural product extracts. Several hit molecules were obtained, most notably a natural product allosteric affector of MEP synthase and a rationally designed bisubstrate derivative of FR900098 (able to associate with both the NADPH and DXP binding sites in MEP synthase). It is particularly noteworthy that allosteric regulation of MEP synthase has not been described previously. Thus, our discovery implicates an alternative site (and new chemical space) for rational drug development.</p></div