Modified Intracellular-Associated Phenotypes in a Recombinant Salmonella Typhi Expressing S. Typhimurium SPI-3 Sequences

A bioinformatics comparison of Salmonella Pathogenicity Island 3 sequences from S. Typhi and S. Typhimurium serovars showed that ten genes are highly conserved. However three of them are pseudogenes in S. Typhi. Our aim was to understand what functions are lost in S. Typhi due to pseudogenes by constructing a S. Typhi genetic hybrid carrying the SPI-3 region of S. Typhimurium instead of its own SPI-3. We observed that under stressful conditions the hybrid strain showed a clear impairment in resistance to hydrogen peroxide and decreased survival within U937 culture monocytes. We hypothesized that the marT-fidL operon, encoded in SPI-3, was responsible for the new phenotypes because marT is a pseudogen in S. Typhi and has a demonstrated role as a transcriptional regulator in S. Typhimurium. Therefore we cloned and transferred the S. Typhimurium marT-fidL operon into S. Typhi and confirmed that invasion of monocytes was dramatically decreased. Finally, our findings suggest that the genomic and functional differences between SPI-3 sequences have implications in the host specificity of Typhi and Typhimurium serovars

Classically time-controlled quantum automata

In this paper we introduce classically time-controlled quantum automata or CTQA, which is a slight but reasonable modification of Moore-Crutchfield quantum finite automata that uses time-dependent evolution operators and a scheduler defining how long each operator will run. Surprisingly enough, time-dependent evolutions provide a significant change in the computational power of quantum automata with respect to a discrete quantum model. Furthermore, CTQA presents itself as a new model of computation that provides a different approach to a formal study of “classical control, quantum data” schemes in quantum computing.CONACYT – Consejo Nacional de Ciencia y TecnologíaPROCIENCI

Growth in fossil and extant deer and implications for body size and life history evolution

© Kolb et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The attached file is the published version of the article

Nocardia asteroides EN ABSCESO PULMONAR INTERPRETADO COMO DE ORIGEN TUBERCULOSO: CASO FATAL.

Se informa el hallazgo de cultivos puros de N.asteroides a partir de la colección purulenta obtenida por drenaje quirúrgico de un absceso en base pulmonar derecha, de 6 cm. de diámetro, en una niña de 13 años de edad con desnutrición de segundo grado y antecedentes de tuberculosis.Lo prolongado de la evolución diagnóstica, el cuadro infeccioso enes inespecífico y el probable deterioro inmunológico pudieron haber producido cierta demora en el diagnóstico y por lo tanto asociarse para el desenlace fatal

Risk factors and perceived restoration in a town destroyed by the 2010 Chile tsunami

A large earthquake and tsunami took place in February 2010, affecting a significant part of the Chilean coast (Maule earthquake, Mw of 8.8). Dichato (37° S), a small town located on Coliumo Bay, was one of the most devastated coastal areas and is currently under reconstruction. Therefore, the objective of this research is to analyze the risk factors that explain the disaster in 2010, as well as perceived restoration 6 years after the event. Numerical modeling of the 2010 Chile tsunami with four nested grids was applied to estimate the hazard. Physical, socioeconomic and educational dimensions of vulnerability were analyzed for pre- and post-disaster conditions. A perceived restoration study was performed to assess the effects of reconstruction on the community. It was focused on exploring the capacity of newly reconstructed neighborhoods to provide restorative experiences in case of disaster. The study was undertaken using the perceived restorativeness scale. The vulnerability variables that best explained the extent of the disaster were housing conditions, low household incomes and limited knowledge about tsunami events, which conditioned inadequate reactions to the emergency. These variables still constitute the same risks as a result of the reconstruction process, establishing that the occurrence of a similar event would result in a similar degree of devastation. For post-earthquake conditions, it was determined that all neighborhoods have the potential to be restorative environments soon after a tsunami. However, some neighborhoods are still located in areas devastated by the 2010 tsunami and again present high vulnerability to future tsunamis

Heterochrony and developmental modularity of cranial osteogenesis in lipotyphlan mammals

Background Here we provide the most comprehensive study to date on the cranial ossification sequence in Lipotyphla, the group which includes shrews, moles and hedgehogs. This unique group, which encapsulates diverse ecological modes, such as terrestrial, subterranean, and aquatic lifestyles, is used to examine the evolutionary lability of cranial osteogenesis and to investigate the modularity of development. Results An acceleration of developmental timing of the vomeronasal complex has occurred in the common ancestor of moles. However, ossification of the nasal bone has shifted late in the more terrestrial shrew mole. Among the lipotyphlans, sequence heterochrony shows no significant association with modules derived from developmental origins (that is, neural crest cells vs. mesoderm derived parts) or with those derived from ossification modes (that is, dermal vs. endochondral ossification). Conclusions The drastic acceleration of vomeronasal development in moles is most likely coupled with the increased importance of the rostrum for digging and its use as a specialized tactile surface, both fossorial adaptations. The late development of the nasal in shrew moles, a condition also displayed by hedgehogs and shrews, is suggested to be the result of an ecological reversal to terrestrial lifestyle and reduced functional importance of the rostrum. As an overall pattern in lipotyphlans, our results reject the hypothesis that ossification sequence heterochrony occurs in modular fashion when considering the developmental patterns of the skull. We suggest that shifts in the cranial ossification sequence are not evolutionarily constrained by developmental origins or mode of ossification

Crocodylian diversity peak and extinction in the late Cenozoic of the northern Neotropics

Northern South America and South East Asia are today’s hotspots of crocodylian diversity with up to six (mainly alligatorid) and four (mainly crocodylid) living species respectively, of which usually no more than two or three occur sympatrically. In contrast, during the late Miocene, 14 species existed in South America. Here we show a diversity peak in sympatric occurrence of at least seven species, based on detailed stratigraphic sequence sampling and correlation, involving four geological formations from the middle Miocene to the Pliocene, and on the discovery of two new species and a new occurrence. This degree of crocodylian sympatry is unique in the world and shows that at least several members of Alligatoroidea and Gavialoidea coexisted. By the Pliocene, all these species became extinct, and their extinction was probably related to hydrographic changes linked to the Andean uplift. The extant fauna is first recorded with the oldest Crocodylus species from South America.Facultad de Ciencias Naturales y Muse

Bottlenose dolphins (Tursiops truncatus) do also cast neutrophil extracellular traps against the apicomplexan parasite Neospora caninum

Neutrophil extracellular traps (NETs) are web-like structures composed of nuclear DNA decorated with histones and cytoplasmic peptides which antiparasitic properties have not previously been investigated in cetaceans. Polymorphonuclear neutrophils (PMN) were isolated from healthy bottlenose dolphins (Tursiops truncatus), and stimulated with Neospora caninum tachyzoites and the NETs-agonist zymosan. In vitro interactions of PMN with the tachyzoites resulted in rapid extrusion of NETs. For the demonstration and quantification of cetacean NETs, extracellular DNA was stained by using either Sytox Orange® or Pico Green®. Scanning electron microscopy (SEM) and fluorescence analyses demonstrated PMN-derived release of NETs upon exposure to tachyzoites of N. caninum. Co-localization studies of N. caninum induced cetacean NETs proved the presence of DNA adorned with histones (H1, H2A/H2B, H3, H4), neutrophil elastase (NE), myeloperoxidase (MPO) and pentraxin (PTX) confirming the molecular properties of mammalian NETosis. Dolphin-derived N. caninum-NETosis were efficiently suppressed by DNase I and diphenyleneiodonium (DPI) treatments. Our results indicate that cetacean-derived NETs represent an ancient, conserved and relevant defense effector mechanism of the host innate immune system against N. caninum and probably other related neozoan parasites circulating in the marine environment

An exceptionally well-preserved skeleton of Palaeothentes from the Early Miocene of Patagonia, Argentina: new insights into the anatomy of extinct paucituberculatan marsupials

International audienc

Internal cranial anatomy of Early Triassic species of †saurichthys (Actinopterygii: †saurichthyiformes): implications for the phylogenetic placement of †saurichthyiforms

© 2018 The Author(s). Background: †Saurichthyiformes were a successful group of latest Permian-Middle Jurassic predatory actinopterygian fishes and constituted important, widely-distributed components of Triassic marine and freshwater faunas. Their systematic affinities have long been debated, with †saurichthyiforms often being aligned with chondrosteans, a group today comprising sturgeons and paddlefishes. However, their character-rich endocranial anatomy has not been investigated in detail since the first half of the 20th century. Since then, major advances have occurred in terms of our understanding of early actinopterygian anatomy, as well as techniques for extracting morphological data from fossils. Results: We used μCT to study the internal cranial anatomy of two of the stratigraphically oldest representatives of †Saurichthys, from the Early Triassic of East Greenland and Nepal. Our work revealed numerous previously unknown characters (e.g., cryptic oticooccipital fissure; intramural diverticula of braincase; nasobasal canals; lateral cranial canal; fused dermohyal), and permitted the reevalution of features relating to the structure of cranial fossae, basicranial circulation and opercular anatomy of the genus. Critically, we reinterpret the former †saurichthyiform opercle as an expanded subopercle. For comparison, we also produced the first digital models of a braincase and endocast of a sturgeon (A. brevirostrum). New information from these taxa was included in a broad phylogenetic analysis of Actinopterygii. †Saurichthyiforms are resolved as close relatives of †Birgeria, forming a clade that constitutes the immediate sister group of crown actinopterygians. However, these and other divergences near the actinopterygian crown node are weakly supported. Conclusions: Our phylogeny disagrees with the historically prevalent hypothesis favoring the chondrostean affinities of †saurichthyiforms. Previously-proposed synapomorphies uniting the two clades, such as the closure of the oticooccipital fissure, the posterior extension of the parasphenoid, and the absence of an opercular process, are all widespread amongst actinopterygians. Others, like those relating to basicranial circulation, are found to be based on erroneous interpretations. Our work renders the †saurichthyiform character complex adequately understood, and permits detailed comparisons with other stem and crown actinopterygians. Our phylogenetic scheme highlights outstanding questions concerning the affinity of many early actinopterygians, such as the Paleozoic-early Mesozoic deep-bodied forms, which are largely caused by lack of endoskeletal data