Developing a site-conditions map for seismic hazard Assessment in Portugal

The evaluation of site effects on a broad scale is a critical issue for seismic hazard and risk assessment, land use planning and emergency planning. As characterization of site conditions based on the shear-wave velocity has become increasingly important, several methods have been proposed in the literature to estimate its average over the first thirty meters (Vs30) from more extensively available data. These methods include correlations with geologic-geographic defined units and topographic slope. In this paper we present the first steps towards the development of a site–conditions map for Portugal, based on a regional database of shear-wave velocity data, together with geological, geographic, and lithological information. We computed Vs30 for each database site and classified it according to the corresponding geological-lithological information using maps at the smallest scale available (usually 1:50000). We evaluated the consistency of Vs30 values within generalized-geological classes, and assessed the performance of expedient methodologies proposed in the literature

Hall effect in laser ablated Co_2(Mn,Fe)Si thin films

Pulsed laser deposition was employed to grow thin films of the Heusler compounds Co_2MnSi and Co_2FeSi. Epitaxial growth was realized both directly on MgO (100) and on a Cr or Fe buffer layer. Structural analysis by x-ray and electron diffraction shows for both materials the ordered L2_1 structure. Bulk magnetization was determined with a SQUID magnetometer. The values agree with the Slater-Pauling rule for half-metallic Heusler compounds. On the films grown directly on the substrate measurements of the Hall effect have been performed. The normal Hall effect is nearly temperature independent and points towards a compensated Fermi surface. The anomalous contribution is found to be dominated by skew scattering. A remarkable sign change of both normal and anomalous Hall coefficients is observed on changing the valence electron count from 29 (Mn) to 30 (Fe).Comment: 9 pages, 6 figures submitted to J Phys

DATS - data containers for web applications

Data containers enable users to control access to their data while untrusted applications compute on it. However, they require replicating an application inside each container - compromising functionality, programmability, and performance. We propose DATS - a system to run web applications that retains application usability and efficiency through a mix of hardware capability enhanced containers and the introduction of two new primitives modeled after the popular model-view-controller (MVC) pattern. (1) DATS introduces a templating language to create views that compose data across data containers. (2) DATS uses authenticated storage and confinement to enable an untrusted storage service, such as memcached and deduplication, to operate on plain-text data across containers. These two primitives act as robust declassifiers that allow DATS to enforce non-interference across containers, taking large applications out of the trusted computing base (TCB). We showcase eight different web applications including Gitlab and a Slack-like chat, significantly improve the worst-case overheads due to application replication, and demonstrate usable performance for common-case usage

Event-based control of basic wastewater treatment plant control loops

[Abstract] This paper addresses the problem of basic control loops in wastewater treatment plants. By basic control loops we are referring to the traditional dissolved oxygen and nitrates. They are the basic controls more sophisticated control solutions can be based upon. Therefore it is important that these loops perform in an efficient way. The problem is addressed here within the framework provided by the Benchmark Simulation Model Number 1 (BSM1) and by the use of an event-based solution. It will be verified that the solution can slightly improve the performance of the already exiting controllers both at loop level as well as at plant operation level.[Resumen] Este documento aborda el problema de los bucles de control básicos en las plantas de tratamiento de aguas residuales. Por bucles de control básicos nos referimos a los tradicionales disueltos de oxígeno y nitratos. Son los controles básicos en los que se pueden basar las soluciones de control más sofisticadas. Por lo tanto, es importante que estos bucles funcionen de manera eficiente. El problema se aborda aquí dentro del marco provisto por el Número de modelo de simulación de referencia (BSM1) y por el uso de una solución basada en eventos. Se verificará que la solución puede mejorar ligeramente el rendimiento de los controladores que ya existen, tanto a nivel de bucle como a nivel de operación de la planta

Shortening and Improving the Embryonic Stem Cell Test through the Use of Gene Biomarkers of Differentiation

The embryonic Stem cell Test (EST) is a validated assay for testing embryotoxicity in vitro. The total duration of this protocol is 10 days, and its main end-point is based on histological determinations. It is suggested that improvements on EST must be focused toward molecular end-points and, if possible, to reduce the total assay duration. Five days of exposure of D3 cells in monolayers under spontaneous differentiation to 50 ng/mL of the strong embryotoxic 5-fluorouracil or to 75 μg/mL of the weak embryotoxic 5,5-diphenylhydeantoin caused between 20 and 74% of reductions in the expression of the following genes: Pnpla6, Afp, Hdac7, Vegfa, and Nes. The exposure to 1 mg/mL of nonembryotoxic saccharin only caused statistically significant reductions in the expression of Nes. These exposures reduced cell viability of D3 cells by 15, 28, and 34%. We applied these records to the mathematical discriminating function of the EST method to find that this approach is able to correctly predict the embryotoxicity of all three above-mentioned chemicals. Therefore, this work proposes the possibility of improve EST by reducing its total duration and by introducing gene expression as biomarker of differentiation, which might be very interesting for in vitro risk assessment embryotoxicity

New findings of Prototherium ausetanum (Mammalia, Pan-Sirenia) from paving stones in Girona (Catalonia, Spain)?

Taxonomic and morphological approaches on Eocene sirenians from Catalonia (Spain) benefit from a newly discovered specimen found in a quite unusual locality, the pedestrian zone in the city of Girona. Two fossil-bearing limestone slabs from middle Eocene (Bartonian) layers of a quarry in the wider surrounding area north-west of Barcelona, were CT-scanned in the Clínica Girona to enhance more detailed investigations. Post-processing of the scans and, as far as possible, 3D-reconstruction of the preserved elements in the slabs was performed at Museum für Naturkunde Berlin. Thereby, a skull of a Dugong specimen was used as a reference point. Based on the combined analysis of macroscopic and CT-data, the specimen most likely represents Prototherium ausetanum Balaguer & Alba, 2016 and complements the available information of the holotype and hitherto only known specimen of that species. The Girona specimen is an adult, but small individual that corroborates P. ausetanum as a generally small-sized species compared to other known Prototherium taxa

One-shot domain adaptation in multiple sclerosis lesion segmentation using convolutional neural networks

In recent years, several convolutional neural network (CNN) methods have been proposed for the automated white matter lesion segmentation of multiple sclerosis (MS) patient images, due to their superior performance compared with those of other state-of-the-art methods. However, the accuracies of CNN methods tend to decrease significantly when evaluated on different image domains compared with those used for training, which demonstrates the lack of adaptability of CNNs to unseen imaging data. In this study, we analyzed the effect of intensity domain adaptation on our recently proposed CNN-based MS lesion segmentation method. Given a source model trained on two public MS datasets, we investigated the transferability of the CNN model when applied to other MRI scanners and protocols, evaluating the minimum number of annotated images needed from the new domain and the minimum number of layers needed to re-train to obtain comparable accuracy. Our analysis comprised MS patient data from both a clinical center and the public ISBI2015 challenge database, which permitted us to compare the domain adaptation capability of our model to that of other state-of-the-art methods. For the ISBI2015 challenge, our one-shot domain adaptation model trained using only a single image showed a performance similar to that of other CNN methods that were fully trained using the entire available training set, yielding a comparable human expert rater performance. We believe that our experiments will encourage the MS community to incorporate its use in different clinical settings with reduced amounts of annotated data. This approach could be meaningful not only in terms of the accuracy in delineating MS lesions but also in the related reductions in time and economic costs derived from manual lesion labeling

Cell-to-cell aggregation in S. epidermidis and its effect on quantification of total and viable bacteria within biofilms

Biofilms forming on the surface of indwelling medical devices by microorganisms such as Staphylococcus epidermidis, act as a source of acute infections. Since colonization of medical devices represents a serious problem in public healthcare-associated infections, bacteria forming biofilms have been an important issue often studied. Proper quantification of viable bacteria within S. epidermidis biofilms can however be challenging. Often, biofilm quantification of S. epidermidis is performed with colorimetric methods but these do not provide information regarding viable bacteria. CFU counting is often used but in the case of S. epidermidis, a bacteria that normally grows in clusters of cells, sonication is always required in order to obtain individual cells. In older biofilms, the number of dormant bacteria is expected to be higher than in young biofilms. Therefore, disrupting a biofilm structure without damage the cells in older biofilms can be a challenge. Here, biofilm samples of Staphylococcus epidermidis 9142 strain grown for 24, 48 or 72H in TSB supplemented with 0,5% glucose were ressuspended 1 mL of physiological saline solution and sonicated at different cycles. Following sonication biofilms were quantified using three different approaches: colorimetric methods, CFU counting, and microscopic evaluation using a Neübauer chamber coupled with staining with fluorescence dye LIVE/DEAD® BacLight ™ Bacterial Viability Kit (Molecular Probes Inc). Cell counting was optimized using Sigma Scan Pro and validated against manual counting of the images. In the conditions used, higher numbers of sonication cycles prevented any clustering of cells but were affecting cell viability. On the other hand, lower numbers of sonication cycles were not effective in completely eliminating cell clusters, especially in 72H-old biofilms. The presence of the cell clusters at the lower sonication cycles resulted in high variability of CFU counting. On the other hand, cell counting with a Neübauer chamber was the best way to proper quantify the total and viable bacteria within the biofilms. By using the automatic counting software and validating the methodology, quantification of biofilms was relatively fast and reliable to perform. Keywords Biofilm; Staphylococcus epidermidis; Sonication, automatic cell countin