Insights into the genetics of the Zhonghua 11 Resistance to Meloidogyne graminicola and its molecular determinism in rice

This research and HN were funded by the Consultative Group for International Agricultural Research Program on rice-agrifood systems (CRP-RICE, 2017–2022), the French National Institute for Sustainable Development (IRD–France), and the International Join Laboratory (LMI-Rice 2) in Vietnam. Funding for some parts of this work was also provided through an SFC ODA GCRF award via the University of St Andrews, United Kingdom. The James Hutton Institute receives funding from the Rural and Environment Science and Analytical Services Division of the Scottish Government.Meloidogyne graminicola is a widely spread nematode pest of rice that reduces crop yield up to 20% on average in Asia, with devastating consequences for local and global rice production. Due to the ban on many chemical nematicides and the recent changes in water management practices in rice agriculture, an even greater impact of M. graminicola can be expected in the future, stressing the demand for the development of new sustainable nematode management solutions. Recently, a source of resistance to M. graminicola was identified in the Oryza sativa japonica rice variety Zhonghua 11 (Zh11). In the present study, we examine the genetics of the Zh11 resistance to M. graminicola and provide new insights into its cellular and molecular mechanisms. The segregation of the resistance in F2 hybrid populations indicated that two dominant genes may be contributing to the resistance. The incompatible interaction of M. graminicola in Zh11 was distinguished by a lack of swelling of the root tips normally observed in compatible interactions. At the cellular level, the incompatible interaction was characterised by a rapid accumulation of reactive oxygen species in the vicinity of the nematodes, accompanied by extensive necrosis of neighbouring cells. The expression profiles of several genes involved in plant immunity were analysed at the early stages of infection during compatible (susceptible plant) and incompatible (resistant plant) interactions. Notably, the expression of OsAtg4 and OsAtg7, significantly increased in roots of resistant plants in parallel with the cell death response, suggesting that autophagy is activated and may contribute to the resistance-mediated hypersensitive response. Similarly, transcriptional regulation of genes involved in hormonal pathways in Zh11 indicated that salicylate signalling may be important in the resistance response towards M. graminicola. Finally, the nature of the resistance to M. graminicola and the potential exploitation of the Zh11 resistance for breeding are discussed.Publisher PDFPeer reviewe

PERSISTENT ORGANIC POLLUTANTS(POPs) IN VIETNAMESE ENVIRONMENT-A REVIEW OF CONTAMINATION, FATE AND TOXIC POTENTIAL

Joint Research on Environmental Science and Technology for the Eart

Prevalence of arterial hypertension in Vietnamese seafarers aboard merchant vessels: a cross-sectional study

Background: Hypertension is one of the leading causes of morbidity and mortality globally. It is a major riskfactor for major cardiovascular events such as stroke, myocardial infarction, heart failure, kidney failure,and blindness. The aim of this research is to assess the prevalence and some factors related to arterialhypertension on Vietnamese seafarers aboard merchant vessels.Materials and methods: Seven hundred eight Vietnamese seafarers working aboard merchant ships wereexamined at the Institute of Marine Medicine before going to sea during the period from January 2022to December 2022. It was a cross-sectional descriptive epidemiological study. The following parameterswere measured: blood pressure, height, weight, waist circumference, buttock circumference to assess theprevalence of hypertension, overweight, and obesity. Seafarers we directly interviewed about workplaceon ships and physical exercise, smoking tobacco, alcohol abuse, and anxiety symptoms to identify severalfactors associated with hypertension.Results: The prevalence of hypertension in seafarers was 32.9%, prehypertension 26.4%, overweight32.4%, obesity 13.3%, abdominal obesity 47.7%. Factors related to hypertension of seafarers included: jobduration at sea > 10 years, odds ratio (OR) = 8.23 (95% confidence interval [CI] 4.34–17.27); non-officers,OR = 2.11 (95% CI 1.45–2.82); engine room crew, OR = 2.11 (95% CI 1.45–3.58); obesity, OR = 3.34(95% CI 2.15–5.63); abdominal obesity, OR = 9.12 (95% CI 4.23–18.45); current smoking, OR = 1.32(95% CI 1.02–1.99); irregular exercise, OR =1.43 (95% CI 1.03–2.18); anxiety symptoms, OR = 1.56 (95%CI 1.08–2.27).Conclusions: Hypertension is a health problem for Vietnamese seafarers. To minimise hypertension, seafarersneed to adjust their lifestyle, increase regular exercise and improve psychological issues on board

ARSENIC POLLUTION IN TUBE WELL WATER AT HANOI SUBURB VILLAGES

Joint Research on Environmental Science and Technology for the Eart

The Study of Isomeric Ratios in Photonuclear Reactions Forming High Spin Isomers in the Giant Dipole Resonance Region

We studied the isomeric ratios  in  odd-odd nuclei $^{196}$Au,$^{182}$Ta  and $^{194}$Ir  with    high spin isomeric states  produced  in $^{197}$Au$(\gamma,  n)$ $^{196m,g}$Au, $^{183}$W$(\gamma,  p)$ $^{182m,g}$Ta  and $^{185}$Pt$(\gamma,  p)$$^{194m,g}$Ir  reactions  by  using  the activation  technique  and  $\gamma$-ray  spectroscopic  method  in  the  giant    dipole  resonance  (GDR)  region.  The high-purity natural Au, W and Pt  foils in disc shape were irradiated with bremsstrahlungs  generated from an  electron  accelerator  Microtron.  The  irradiated  foils  were  measured  by  the  high  resolution  $\gamma$-ray spectroscopic system which consists of a Ge(HP) detector and a multichannel analyzer. In order to improve the  accuracy  of  the  experimental  results,  necessary  corrections  were  made  in  the  $\gamma$-ray  activity measurements and data analysis.  The results were  analyzed,  discussed and compared with those of other authors  as well as with theoretical model calculations.  The study shows that the isomeric ratios in  nuclei with high spin isomeric states are much lower than that in low spin isomeric state isomers

ARSENIC POLLUTION IN GROUNDWATER IN RED RIVER DELTA, VIETNAM : SITUATION AND HUMAN EXPOSURE

Joint Research on Environmental Science and Technology for the Eart

Rifampicin resistant 'Mycobacterium tuberculosis' in Vietnam, 2020–2022

Objective: We conducted a descriptive analysis of multi-drug resistant tuberculosis (MDR-TB) in Vietnam’s two largest cities, Hanoi and Ho Chi Minh city. Methods: All patients with rifampicin resistant tuberculosis were recruited from Hanoi and surrounding provinces between 2020 and 2022. Additional patients were recruited from Ho Chi Minh city over the same time period. Demographic data were recorded from all patients, and samples collected, cultured, whole genome sequenced and analysed for drug resistance mutations. Genomic susceptibility predictions were made on the basis of the World Health Organization’s catalogue of mutations in Mycobacterium tuberculosis associated with drug resistance, version 2. Comparisons were made against phenotypic drug susceptibility test results where these were available. Multivariable logistic regression was used to assess risk factors for previous episodes of tuberculosis. Results: 233/265 sequenced isolates were of sufficient quality for analysis, 146 (63 %) from Ho Chi Minh City and 87 (37 %) from Hanoi. 198 (85 %) were lineage 2, 20 (9 %) were lineage 4, and 15 (6 %) were lineage 1. 17/211 (8 %) for whom HIV status was known were infected, and 109/214 (51 %) patients had had a previous episode of tuberculosis. The main risk factor for a previous episode was HIV infection (odds ratio 5.1 (95 % confidence interval 1.3–20.0); p = 0.021). Sensitivity for predicting first-line drug resistance from whole genome sequencing data was over 90 %, with the exception of pyrazinamide (85 %). For moxifloxacin and amikacin it was 50 % or less. Among rifampicin-resistant isolates, prevalence of resistance to each non-first-line drug was < 20 %. Conclusions: Drug resistance among most MDR-TB strains in Vietnam’s two largest cities is confined largely to first-line drugs. Living with HIV is the main risk factor among patients with MDR-TB for having had a previous episode of tuberculosis

Host Transcription Profile in Nasal Epithelium and Whole Blood of Hospitalized Children Under 2 Years of Age With Respiratory Syncytial Virus Infection.

BACKGROUND: Most insights into the cascade of immune events after acute respiratory syncytial virus (RSV) infection have been obtained from animal experiments or in vitro models. METHODS: In this study, we investigated host gene expression profiles in nasopharyngeal (NP) swabs and whole blood samples during natural RSV and rhinovirus (hRV) infection (acute versus early recovery phase) in 83 hospitalized patients <2 years old with lower respiratory tract infections. RESULTS: Respiratory syncytial virus infection induced strong and persistent innate immune responses including interferon signaling and pathways related to chemokine/cytokine signaling in both compartments. Interferon-α/β, NOTCH1 signaling pathways and potential biomarkers HIST1H4E, IL7R, ISG15 in NP samples, or BCL6, HIST2H2AC, CCNA1 in blood are leading pathways and hub genes that were associated with both RSV load and severity. The observed RSV-induced gene expression patterns did not differ significantly in NP swab and blood specimens. In contrast, hRV infection did not as strongly induce expression of innate immunity pathways, and significant differences were observed between NP swab and blood specimens. CONCLUSIONS: We conclude that RSV induced strong and persistent innate immune responses and that RSV severity may be related to development of T follicular helper cells and antiviral inflammatory sequelae derived from high activation of BCL6