Somatic development and embryo yield in crossbred F1 mice generated by different mating strategies

The aim of this study was to evaluate different mating strategies among endogamic strains to create F1 populations of mice, minimising the effect of inbreeding depression on somatic development and embryo yield. Females from the strains Swiss, CBA and C57Bl/6 were divided in nine experimental mate arrangements. The total numbers of pups born alive per dam and somatic development, estimated by weighing and measuring the crown-rump length, were recorded. Superovulation response was evaluated in outbreed females. Litter size differed among endogamic dams, irrespective of the sire. Somatic development results suggest heterosis and imprinting phenomena, once a differential parental effect was demonstrated. There was no difference in corpora lutea, ova or embryos recovered (P > 0.05), but recovery and viability rates differ among F1 groups (P < 0.05). The association of dam prolificity with somatic development and superovulation response of the pups should be considered for experimental F1 populations establishment. The use of outbreed animals, however, did not reduce response variability to hormone treatment

Somatic development and embryo yield in crossbred F1 mice generated by different mating strategies

The aim of this study was to evaluate different mating strategies among endogamic strains to create F1 populations of mice, minimising the effect of inbreeding depression on somatic development and embryo yield. Females from the strains Swiss, CBA and C57Bl/6 were divided in nine experimental mate arrangements. The total numbers of pups born alive per dam and somatic development, estimated by weighing and measuring the crown-rump length, were recorded. Superovulation response was evaluated in outbreed females. Litter size differed among endogamic dams, irrespective of the sire. Somatic development results suggest heterosis and imprinting phenomena, once a differential parental effect was demonstrated. There was no difference in corpora lutea, ova or embryos recovered (P > 0.05), but recovery and viability rates differ among F1 groups (P < 0.05). The association of dam prolificity with somatic development and superovulation response of the pups should be considered for experimental F1 populations establishment. The use of outbreed animals, however, did not reduce response variability to hormone treatment

Validação de um histórico de enfermagem para unidade de terapia intensiva pediátrica

RESUMO Objetivo Validar o Histórico de Enfermagem para uma unidade de terapia intensiva pediátrica quanto à aparência e conteúdo. Métodos Estudo metodológico para validação de histórico de enfermagem para hospital de ensino na região noroeste do Paraná. O instrumento, construído em etapa anterior, foi elaborado com informações obtidas em revisão de literatura pertinente e elementos empíricos apontados por enfermeiros que vivenciam essas unidades. A versão provisória foi submetida a um painel de juízes (março-novembro/2014), cuja expertise contribuiu para avaliação de aparência e conteúdo (análise, readequação, refinamento, finalização). Resultados O critério Pertinência de Conteúdo, relativo aos tópicos do instrumento, atingiu os maiores níveis de concordância entre os juízes (78-100%). Os tópicos que não atingiram a concordância pré-definida foram substituídos, excluídos ou reformulados. O instrumento reestruturado foi reavaliado pelos juízes, obtendo concordância satisfatória em todos os critérios (78-100%). Conclusões A versão definitiva foi validada quanto à aparência e conteúdo, podendo ser utilizada