Nurture commodified? An investigation into commercial human milk supply chains

The material conditions in which women provide breast milk range widely, on the basis of their class and geographical provenance. The commercialisation of breast milk provision throws up questions related to debates on the transnational reconfiguration of social reproduction as they intersect with discourses on motherhood and healthy child development as well as contemporary processes of commodification of the body and the emergence of new gendered forms of atypical work in the global economy (Bakker 2007; LeBaron 2010; Steans & Tepe 2010). This article presents a study of the first commercial human milk processor in India, NeoLacta Lifesciences that obtained an export license for the Australian market in 2017. These practices may be seen to be part of a wider Reproductive Industrial Complex (Vertommen 2016), in which women’s reproductive bodily capacities are enrolled in wider economic and financial processes, instantiating new relations between gender, race, economies and care. This article employs a feminist political economy framework that places into dialogue analyses of social reproduction and commodification with feminist science/technology studies and medical/political anthropology in order to analyse the social, political, and technical processes that transform breast milk into a commodity that is internationally traded and the implications of this for contemporary understandings of work and gender

High-confidence glycosome proteome for procyclic form <em>Trypanosoma brucei</em> by epitope-tag organelle enrichment and SILAC proteomics

The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed

Evaluation of chloroform/methanol extraction to facilitate the study of membrane proteins of non-model plants

Membrane proteins are of great interest to plant physiologists because of their important function in many physiological processes. However, their study is hampered by their low abundance and poor solubility in aqueous buffers. Proteomics studies of non-model plants are generally restricted to gel-based methods. Unfortunately, all gel-based techniques for membrane proteomics lack resolving power. Therefore, a very stringent enrichment method is needed before protein separation. In this study, protein extraction in a mixture of chloroform and methanol in combination with gel electrophoresis is evaluated as a method to study membrane proteins in non-model plants. Benefits as well as disadvantages of the method are discussed. To demonstrate the pitfalls of working with non-model plants and to give a proof of principle, the method was first applied to whole leaves of the model plant Arabidopsis. Subsequently, a comparison with proteins extracted from leaves of the non-model plant, banana, was made. To estimate the tissue and organelle specificity of the method, it was also applied on banana meristems. Abundant membrane or lipid-associated proteins could be identified in both tissues, with the leaf extract yielding a higher number of membrane proteins