491 research outputs found
Variations in Stellar Clustering with Environment: Dispersed Star Formation and the Origin of Faint Fuzzies
The observed increase in star formation efficiency with average cloud
density, from several percent in whole giant molecular clouds to ~30 or more in
cluster-forming cores, can be understood as the result of hierarchical cloud
structure if there is a characteristic density as which individual stars become
well defined. Also in this case, the efficiency of star formation increases
with the dispersion of the density probability distribution function (pdf).
Models with log-normal pdf's illustrate these effects. The difference between
star formation in bound clusters and star formation in loose groupings is
attributed to a difference in cloud pressure, with higher pressures forming
more tightly bound clusters. This correlation accounts for the observed
increase in clustering fraction with star formation rate and with the
observation of Scaled OB Associations in low pressure environments. ``Faint
fuzzie'' star clusters, which are bound but have low densities, can form in
regions with high Mach numbers and low background tidal forces. The proposal by
Burkert, Brodie & Larsen (2005) that faint fuzzies form at large radii in
galactic collisional rings, satisfies these constraints.Comment: 14 pages, 2 figures, ApJ, 672, January 10th 200
Repositioning the Catalytic Triad Aspartic Acid of Haloalkane Dehalogenase: Effects on Stability, Kinetics, and Structure
Haloalkane dehalogenase (DhlA) catalyzes the hydrolysis of haloalkanes via an alkyl-enzyme intermediate. The covalent intermediate, which is formed by nucleophilic substitution with Asp124, is hydrolyzed by a water molecule that is activated by His289. The role of Asp260, which is the third member of the catalytic triad, was studied by site-directed mutagenesis. Mutation of Asp260 to asparagine resulted in a catalytically inactive D260N mutant, which demonstrates that the triad acid Asp260 is essential for dehalogenase activity. Furthermore, Asp260 has an important structural role, since the D260N enzyme accumulated mainly in inclusion bodies during expression, and neither substrate nor product could bind in the active-site cavity. Activity for brominated substrates was restored to D260N by replacing Asn148 with an aspartic or glutamic acid. Both double mutants D260N+N148D and D260N+N148E had a 10-fold reduced kcat and 40-fold higher Km values for 1,2-dibromoethane compared to the wild-type enzyme. Pre-steady-state kinetic analysis of the D260N+N148E double mutant showed that the decrease in kcat was mainly caused by a 220-fold reduction of the rate of carbon-bromine bond cleavage and a 10-fold decrease in the rate of hydrolysis of the alkyl-enzyme intermediate. On the other hand, bromide was released 12-fold faster and via a different pathway than in the wild-type enzyme. Molecular modeling of the mutant showed that Glu148 indeed could take over the interaction with His289 and that there was a change in charge distribution in the tunnel region that connects the active site with the solvent. On the basis of primary structure similarity between DhlA and other α/ÎČ-hydrolase fold dehalogenases, we propose that a conserved acidic residue at the equivalent position of Asn148 in DhlA is the third catalytic triad residue in the latter enzymes.
Computational Controversy
Climate change, vaccination, abortion, Trump: Many topics are surrounded by
fierce controversies. The nature of such heated debates and their elements have
been studied extensively in the social science literature. More recently,
various computational approaches to controversy analysis have appeared, using
new data sources such as Wikipedia, which help us now better understand these
phenomena. However, compared to what social sciences have discovered about such
debates, the existing computational approaches mostly focus on just a few of
the many important aspects around the concept of controversies. In order to
link the two strands, we provide and evaluate here a controversy model that is
both, rooted in the findings of the social science literature and at the same
time strongly linked to computational methods. We show how this model can lead
to computational controversy analytics that have full coverage over all the
crucial aspects that make up a controversy.Comment: In Proceedings of the 9th International Conference on Social
Informatics (SocInfo) 201
On the Rapid Collapse and Evolution of Molecular Clouds
Stars generally form faster than the ambipolar diffusion time, suggesting
that several processes short circuit the delay and promote a rapid collapse.
These processes are considered here, including turbulence compression in the
outer parts of giant molecular cloud (GMC) cores and GMC envelopes, GMC core
formation in an initially supercritical state, and compression-induced
triggering in dispersing GMC envelopes. The classical issues related to star
formation timescales are addressed: high molecular fractions, low efficiencies,
long consumption times for CO and HCN, rapid GMC core disruption and the lack
of a stable core, long absolute but short relative timescales with accelerated
star formation, and the slow motions of protostars. We consider stimuli to
collapse from changes in the density dependence of the ionization fraction, the
cosmic ray ionization rate, and various dust properties at densities above
~10^5 cm^{-3}. We favor the standard model of subcritical GMC envelops and
suggest they would be long lived if not for disruption by rapid star formation
in GMC cores. The lifecycle of GMCs is illustrated by a spiral arm section in
the Hubble Heritage image of M51, showing GMC formation, star formation, GMC
disruption with lingering triggered star formation, and envelope dispersal.
There is no delay between spiral arm dustlanes and star formation; the
classical notion results from heavy extinction in the dust lane and triggered
star formation during cloud dispersal. Differences in the IMF for the different
modes of star formation are considered.Comment: 46 pages, 5 figures, scheduled for ApJ 668, October 20, 200
The effect of the dynamical state of clusters on gas expulsion and infant mortality
The star formation efficiency (SFE) of a star cluster is thought to be the
critical factor in determining if the cluster can survive for a significant
(>50 Myr) time. There is an often quoted critical SFE of ~30 per cent for a
cluster to survive gas expulsion. I reiterate that the SFE is not the critical
factor, rather it is the dynamical state of the stars (as measured by their
virial ratio) immediately before gas expulsion that is the critical factor. If
the stars in a star cluster are born in an even slightly cold dynamical state
then the survivability of a cluster can be greatly increased.Comment: 6 pages, 2 figures. Review talk given at the meeting on "Young
massive star clusters - Initial conditions and environments", E. Perez, R. de
Grijs, R. M. Gonzalez Delgado, eds., Granada (Spain), September 2007,
Springer: Dordrecht. Replacement to correct mistake in a referenc
Characterizing generated charged inverse micelles with transient current measurements
We investigate the generation of charged inverse micelles in nonpolar surfactant solutions relevant for applications such as electronic ink displays and liquid toners. When a voltage is applied across a thin layer of a nonpolar surfactant solution between planar electrodes, the generation of charged inverse micelles leads to a generation current. From current measurements it appears that such charged inverse micelles generated in the presence of an electric field behave differently compared to those present in equilibrium in the absence of a field. To examine the origin of this difference, transient current measurements in which the applied voltage is suddenly increased are used to measure the mobility and the amount of generated charged inverse micelles. The mobility and the corresponding hydrodynamic size are found to be similar to those of charged inverse micelles present in equilibrium, which indicates that other properties determine their different behavior. The amplitude and shape of the transient currents measured as a function of the surfactant concentration confirm that the charged inverse micelles are generated by bulk disproportionation. A theoretical model based on bulk disproportionation with simulations and analytical approximations is developed to analyze the experimental transient currents
The Role of Dectin-2 for Host Defense Against Disseminated Candidiasis
Acknowledgments This work was supported by European Union ALLFUN (FP7/2007 2013, HEALTH-2010-260338) (Fungi in the setting of inflammation, allergy and autoimmune diseases: Translating basic science into clinical practices ââALLFUNââ) to D.C.I., F.C., C.F., M.G.N., and N.A.R.G. M.G.N and J.Q. were supported by a Vici grant of The Netherlands Organization of Scientific Research (to M.G.N.). M.G.N. was supported by an ERC Consolidator Grant (nr. 310372). N.A.R.G. was also supported by the Wellcome Trust (086827, 075470, 097377, & 101873).Peer reviewedPublisher PD
Weâve Been Down this Road Before: Evidence on the Health Consequences of Precarious Employment in Industrial Societies, 1840-1920
A large body of international scientific research now indicates that the growth of job insecurity, flexible/temporary work and precarious forms of self-employment have had significant negative consequences for occupational health and safety. What is often overlooked in debates over the âchanging world of workâ is that todayâs widespread use insecure and short term work is not new but represents a return to something more resembling labour markets in Australia, Europe and North America in the 19th and early 20th century. As this paper will seek to show, not only were precarious and exploitive working arrangements common during this period but the adverse effects of these on the health, safety and wellbeing was well documented in government inquiries, medical research, press reports and a variety of other sources. Drawing primarily on Australian and British sources, attention here will focus on casual labourers, sweated garment workers, the self-employed and merchant seamen. The paper highlights the valuable role historical research can play in shedding light on contemporary problems and policy debates.The symposium is organised on behalf of AAHANZBS by the Business and Labour History Group, The University of Sydney, with the financial support of the Universityâs Faculty of Economics and Business
Effects of whole-body vibration on postural control in elderly: a systematic review and meta-analysis
<p>Abstract</p> <p>Background</p> <p>This systematic review was performed to summarize the current evidence for whole body vibration (WBV) interventions on postural control in elderly.</p> <p>Methods</p> <p>English and German language papers in Medline, PEDro, Cinahl and the Cochrane databases were searched. Two reviewers extracted data on patients' characteristics, type of WBV intervention and outcomes. Two independent reviewers rated the methodological quality of these studies. Data were pooled using random-effects meta-analysis.</p> <p>Results</p> <p>Fifteen papers reporting quantitative data were included. Results from 15 papers could be pooled for a meta-analysis. The studies involved 933 participants. In 7 studies the authors investigated the effects of vibration plates generating vertical sinusoidal vibrations (VS-WBV) and 7 papers described the use of side-alternating sinusoidal vibrations (SS-WBV). One study investigated both VS-WBV and SS-WBV.</p> <p>Weak to moderate evidence of an overall effect as a result of VS-WBV and SS-WBV was observed for (a) static balance for post-intervention values with a standardized mean difference (SMD) -0.06, 95% CI -0.31 to 0.18 and for change values SMD -0.26, 95% CI -1.09 to 0.57, and (b) dynamic balance for post-intervention-values SMD -0.34, 95% CI -0.60 to -0.08. For functional balance (c) an overall outcome for post-intervention values with SMD of 0.34, 95% CI -0.19 to 0.87 was found.</p> <p>Conclusions</p> <p>The 15 studies reviewed were of moderate methodological quality. In summary, SS-WBV seems to have a beneficial effect on dynamic balance in elderly individuals. However, the current results should be interpreted with caution because of the observed heterogeneity of training parameters and statistical methods. Future studies are warranted to evaluate the effects of WBV on postural control in an elderly population.</p
- âŠ