A multi-element psychosocial intervention for early psychosis (GET UP PIANO TRIAL) conducted in a catchment area of 10 million inhabitants: study protocol for a pragmatic cluster randomized controlled trial

Multi-element interventions for first-episode psychosis (FEP) are promising, but have mostly been conducted in non-epidemiologically representative samples, thereby raising the risk of underestimating the complexities involved in treating FEP in 'real-world' services

Elimination of altered erytrocytes in young and old rats

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Elimination of altered erythrocytes in young and old rats

No abstract availabl

IDENTIFICAZIONE DI CELLULE FETALI NEL SANGUE MATERNO: USO DI ANTICORPI ANTI-ERITROCITI DI FUNICOLO

XX RIUNIONE, ANCONA, 1-2 DICEMBRE 199

Accelerated elimination from the circulation of homologous aged red blood cells in rats bearing anti-spectrin antibodies

In order to analyse a possible role of anti-spectrin antibodies in the clearance of aged red blood cells (RBC), a homologous system was employed, whereby a population of aged RBC, obtained by hypertransfusion, was injected into rats bearing a high level of anti-spectrin antibodies, following immunization with spectrin. The aged RBC bound the anti-spectrin antibodies 'in vitro' and were eliminated from circulation in spectrin-treated rats at a faster rate than in control rats with naturally occurring antibodies. The analysis of the clearance curves revealed aged RBC of heterogeneous lifespans: two principal populations of short- and longer-living could be identified. In rats with anti-spectrin antibodies, the survival of the short-living population was further reduced. However, the similar kinetics of elimination of aged RBC in the two groups (with naturally-occurring and induced antibodies, respectively) suggest that anti-spectrin antibodies strengthened the intervention of the naturally-occurring ones. On the basis of these results, we assume that during their aging in circulation, RBC can accumulate surface alterations to make spectrin accessible to antibodies so that, in addition to anti-band 3 antibodies, anti-spectrin antibodies may contribute to their elimination

COMPONENTI CRIPTICHE DELLA MEMBRANA ERITROCITARIA SONO ESPRESSE IN SUPERFICIE SU ALCUNI ERITROCITI FETALI E A LIVELLO DI PLACENTA E AMNIOS

none13XIX RIUNIONE, FERRARA, 1-2 DICEMBRE 1994noneLUNGHI M; A. GIULIANI; MELANDRI P; TORBOLI M; VESCE F; CAVAZZINI L; FERRON P; STABELLINI G; GRALDI G; PORA R; UNIS L; VERENINI M; BERTI GLunghi, M; Giuliani, Anna Lisa; Melandri, P; Torboli, M; Vesce, F; Cavazzini, L; Ferron, P; Stabellini, G; Graldi, G; Pora, R; Unis, L; Verenini, M; Berti, G

RABBIT IgG ANTIBODIES AGAINST CORD RED BLOOD CELL MEMBRANES BIND TO COMPLEMENT RECEPTOR 1 (CD35)

We have previously shown that a subpopulation of cord/fetal red blood cells (RBC) binds rabbit IgG antibodies raised against cord RBC and absorbed on adult RBC (F-IgG), while control IgG, raised against and absorbed on adult RBC (A-IgG), fails to do so. In the present study, F-IgG maintained its binding to cord RBC surface antigens following absorption on spectrin but not after absorption on skeleton-stripped RBC membranes. Spectrin-absorbed F-IgG- but not A-IgG-affinity-purified material from cord RBC contained polypeptides with apparent MW of complement receptor 1 (CR1) allotypes. Moreover, on immunoblotting these polypeptides reacted with 125I-F-IgG as well as with 125I-anti-CR1 mAb, and binding of 125I-anti-CR1 mAb was inhibited by unlabelled F-IgG. In addition, cord RBC incubated with F-IgG prior to reaction with anti-CR1 showed decreased fluorescence intensity on flow cytometry. Taken together the results suggest that F-IgG binds to CR1 which shows increased expression/accessibility on a subpopulation of cord/fetal RBC