Dietary sugars analysis: quantification of fructooligossacharides during fermentation by HPLC-RI method

In this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1F-fructofuranosylnystose). It was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using Aspergillus aculeatus. This analytical technique may be easily implemented at laboratorial or industrial scale for fructooligossacharides mass-production monitoring allowing also controlling the main substrate (sucrose) and the secondary by-products (glucose and fructose). The proposed chromatographic method had a satisfactory intra- and inter-day variability (in general, with a relative standard deviation lower than 5%), high sensitivity for each sugar (usually, with a relative error lower than 5%), and low detection (lower than 0.06 ± 0.04 g/L) and quantification (lower than 0.2 ± 0.1 g/L) limits. The correct quantification of fructooligossacharides in fermentative media may allow a more precise nutritional formulation of new functional foods, since it is reported that different fructooligossacharides exhibit different biological activities and effects.This work was partially co-financed by FCT and FEDER under Program COMPETE (Project PEst-C/EQB/LA0020/2013), by the Strategic Project PEst-OE/EQB/LA0023/2013 and by the project ref. RECI/BBB-EBI/0179/2012 (project number FCOMP 01-0124-FEDER-027462) funded by Fundação para a Ciência e a Tecnologia

Tools for the co-designing of housing transformations: A study on interaction and visualization modes

In this paper we present and assess tools for visualizing architectonic modifications of existing housing in co-design projects with inhabitants. These tools should enable inhabitants to explore and understand design variations of alterations of their houses. This contribution is part of ongoing research on the use of artificial realities for supporting the transformations of existing housing in architectonically responsible ways. Such transformations may be needed after the delivery of housing, say after five years or later, due to changed regulation, the need of updates or changed living conditions of inhabitants. For arriving at architectonically responsible transformations, we use shape grammar system for defining possible modifications of the housing. For empowering inhabitants to understand and explore these modifications to their housing, we develop a transformation grammar tool—MyChanges—to visualize the modifications by three visualization modes, from fully immersive to non-immersive. Interviews and tests with real inhabitants were performed, and preliminary conclusions show that a tool like the MyChanges would have a good acceptance among inhabitants.info:eu-repo/semantics/acceptedVersio

Crohn's disease activity index and Vienna classification - Is it worthwhile to calculate before surgery?

Background: Crohn's disease (CD) patients with increased disease activity may reveal an increased risk for perioperative complications. The `Crohn's disease activity index' (CDAI) and the `Vienna classification' (VC) were developed for standardized disease activity estimations. The significance of these scores to predict extent, type and early outcome of surgery in CD patients was analyzed. Methods: In 179 surgically treated CD patients, the CDAI and VC were assessed from a prospective database. Relations of the scores with CD risk factors, type, number, location and complications of surgery were analyzed. Results: VC behavior and location subtypes were associated with distinct types of surgery (i.e. `strictureplasty' in `stricturing disease', `colon surgery' in `colon involvement'), but not with surgery type and extent or outcome. Surgery extent (i.e. with 5 vs. 3 `surgical sites' 425 +/- 25 vs. 223.3 +/- 25) and complications (357.1 +/- 36.9 (with) vs. 244.4 +/- 13 (without)) were associated with elevated CDAI levels; however, nicotine abuse remained the only significant risk factor for perioperative complications after multiple logistic regression. Conclusion: The significance of VC or CDAI for predicting the extent of surgery or complications is limited. None of the tested variables except preoperative nicotine abuse influenced the likelihood for perioperative complications. Copyright (c) 2006 S. Karger AG, Base

O conhecimento matemático dos estudantes no início da Licenciatura em Educação Básica: um projeto envolvendo três Escolas superiores de educação

O novo modelo de formação inicial (Decreto-Lei 43/2007) exige que os futuros professores do 1.º e 2.º ciclos do ensino básico e os futuros educadores de infância façam pelo menos 30 ECTS de formação em Matemática na Licenciatura em Educação Básica (LEB), mas a forma e o conteúdo desta formação é da responsabilidade de cada instituição, que define as unidades curriculares, o seu conteúdo e a forma como são lecionadas. Sabe-se que, para além do conteúdo, a forma como o professor aprende tem uma forte influência na forma como vai ensinar. Assim, todos estes aspetos precisam de ser discutidos, tendo por base a investigação já realizada em Portugal e noutros países. Partindo da assunção de que o conhecimento do professor constitui um fator decisivo na interpretação e implementação do currículo e da necessidade de uma discussão alargada de qual deverá ser o conteúdo da formação em Matemática na LEB, as Escolas Superiores de Educação de Lisboa, de Viana do Castelo e de Viseu iniciaram um projeto de investigação que tem como principal objetivo compreender de que modo a formação inicial contribui para o desenvolvimento do conhecimento do professor em Matemática e em Ensino da Matemática e como pode este ser promovido. Uma das questões que o projeto visa investigar é que conhecimento de conteúdo matemático têm os estudantes quando iniciam o curso da LEB. Para caracterizar o conhecimento matemático dos estudantes da LEB, à entrada no curso, foi elaborado um teste diagnóstico, que foi aplicado nas três Escolas Superiores de Educação, em outubro de 2011, a todos os alunos a iniciar o 1.º ano, num total de 268: 143 em Lisboa, 51 em Viseu e 74 em Viana do Castelo. Neste artigo é apresentada uma análise dos principais resultados deste teste bem como as questões e dilemas que aqueles resultados nos colocam

Oxidative precipitation synthesis of calcium-doped manganese ferrite nanoparticles for magnetic hyperthermia

Superparamagnetic nanoparticles are of high interest for therapeutic applications. In this work, nanoparticles of calcium-doped manganese ferrites (CaxMn1−xFe2O4) functionalized with citrate were synthesized through thermally assisted oxidative precipitation in aqueous media. The method provided well dispersed aqueous suspensions of nanoparticles through a one-pot synthesis, in which the temperature and Ca/Mn ratio were found to influence the particles microstructure and morphology. Consequently, changes were obtained in the optical and magnetic properties that were studied through UV-Vis absorption and SQUID, respectively. XRD and Raman spectroscopy studies were carried out to assess the microstructural changes associated with stoichiometry of the particles, and the stability in physiological pH was studied through DLS. The nanoparticles displayed high values of magnetization and heating efficiency for several alternating magnetic field conditions, compatible with biological applications. Hereby, the employed method provides a promising strategy for the development of particles with adequate properties for magnetic hyperthermia applications, such as drug delivery and cancer therapy.This work was funded by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding of CF-UM-UP (UIDB/04650/2020, UIDP/04650/2020), CQUM (UIDB/00686/2020), CICECO Aveiro Institute of Materials (UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020) and by Ministerio de Economía y Competitividad de España (PID2020-113704RB-I00 and PID2020-119242RB-I00), Xunta de Galicia (Centro Singular de Investigación de Galicia—Accreditation 2019-2022 ED431G 2019/06 and IN607A 2018/5 and project ED431C 2020-06), and European Union (EU-ERDF Interreg V-A—Spain-Portugal 0245_IBEROS_1_E, 0712_ACUINANO_1_E, and 0624_2IQBIONEURO_6_E, and Interreg Atlantic Area NANOCULTURE 1.102.531), and the European Union H2020-MSCA-RISE-2019 PEPSA-MATE project. S.R.S. (872233) Veloso acknowledges FCT for a PhD grant (SFRH/BD/144017/2019). Support from MAP-Fis Doctoral Programme is also acknowledged

Magnetoliposomes incorporated in peptide-based hydrogels: towards development of magnetolipogels

A major problem with magnetogels is the encapsulation of hydrophobic drugs. Magnetoliposomes not only provide these domains but also improve drug stability and avert the aggregation of the magnetic nanoparticles. In this work, two magnetoliposome architectures, solid and aqueous, were combined with supramolecular peptide-based hydrogels, which are of biomedical interest owing to their biocompatibility, easy tunability, and wide array of applications. This proof-of-concept was carried out through combination of magnetoliposomes (loaded with the model drug curcumin and the lipid probe Nile Red) with the hydrogels prior to pH triggered gelation, and fluorescence spectroscopy was used to assess the dynamics of the encapsulated molecules. These systems allow for the encapsulation of a wider array of drugs. Further, the local environment of the encapsulated molecules after gelation is unaffected by the used magnetoliposome architecture. This system design is promising for future developments on drug delivery as it provides a means to independently modify the components and adapt and optimize the design according to the required conditions.FCT -Fundação para a Ciência e a Tecnologia(UIDB/00686/2020

Muonic hydrogen cascade time and lifetime of the short-lived 2S2S state

Metastable ${2S}$ muonic-hydrogen atoms undergo collisional ${2S}$-quenching, with rates which depend strongly on whether the $\mu p$ kinetic energy is above or below the ${2S}\to {2P}$ energy threshold. Above threshold, collisional ${2S} \to {2P}$ excitation followed by fast radiative ${2P} \to {1S}$ deexcitation is allowed. The corresponding short-lived $\mu p ({2S})$ component was measured at 0.6 hPa $\mathrm{H}_2$ room temperature gas pressure, with lifetime $\tau_{2S}^\mathrm{short} = 165 ^{+38}_{-29}$ ns (i.e., $\lambda_{2S}^\mathrm{quench} = 7.9 ^{+1.8}_{-1.6} \times 10^{12} \mathrm{s}^{-1}$ at liquid-hydrogen density) and population $\epsilon_{2S}^\mathrm{short} = 1.70^{+0.80}_{-0.56}$ % (per $\mu p$ atom). In addition, a value of the $\mu p$ cascade time, $T_\mathrm{cas}^{\mu p} = (37\pm5)$ ns, was found.Comment: 4 pages, 3 figure