17 research outputs found
PovrÅ”inski epitel jajnika hipotireoidnih novoroÄenih i neonatalnih pacova - iz perspektive PCNA i kaspaze-3
Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration and remodelling after each ovulation during the whole reproductive period. This process increases the risk of one of the most common ovarian tumors in women and the female dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation and cell death during folliculogenesis in the early neonatal period, we aimed to analyze its effect on OSE morphology and dynamics. Materials and Methods. The study was performed on newborn (24-h-old) and neonatal (4-day-old) female rats, a randomized trial between the control and hypothyroid groups, born under controlled circumstances and hypothyroid mothers, respectively. Their ovaries were analyzed histologically and processed to determine the OSE cell height as an average value of four measurement points. Also, the immunopositivity of the proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively. Results and Conclusions. No major structural differences of OSE were found between groups within the given ages except for a slight increment of OSE cell height and incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid animals of both ages in comparison to the controls. Moreover, only scarce OSE cells were caspase-3 positive in both groups and ages, with no difference in immunopositivity. Our study confirms the impact of hypothyroidism in the early postnatal period on morphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent cell removal, which may serve as a premise for future investigation of potential carcinogenesis, in terms of prevention and treatment of ovarian cancer.Uvod. Tokom reproduktivnog perioda, nakon svake ovulacije, povrÅ”inski epitel (PE) jajnika se intenzivno regeneriÅ”e i remodelira. Ovaj proces poveÄava rizik od nastajanja jednog od najÄeÅ”Äih tumora jajnika kod žena i kuja. ImajuÄi u vidu Äinjenicu da hipotireoidizam majki u velikoj meri utiÄe na proliferaciju i Äelijsku smrt u toku folikulogeneze u ranom postnatalnom periodu, cilj rada je bio da analiziramo efekat hipotireoidizma majki na morfologiju i dinamiku PE jajnika potomaka. Materijal i metode. U eksperimentu su koriÅ”Äeni tek roÄeni (24 Äasa stari) i neonatalni (4 dana stari) mladunci koji potiÄu od kontrolnih i hipotireoidnih majki. Morfometrijskom analizom odreÄivana je proseÄna visina Äelija PE, izmerena na Äetiri mesta na svakom ispitivanom preseku jajnika. TakoÄe, imunopozitivnost proliferativnivnog Äelijskog nuklearnog antigena (PCNA) i kaspaze 3 je odreÄivana semikvantitativno. Rezultati i zakljuÄci. VeÄe promene u strukturi PE jajnika nisu zapažene izmeÄu grupa, osim blagog poveÄanja visine Äelija i nepotpune apikalne membrane sa projekcijama citoplazme kod hipotireoidnih životinja. Imunopozitivnost PCNA je znaÄajno poveÄana u jajnicima hipotireoidnih životinja obe starosne grupe u odnosu na kontrole, dok je imunopozitivnost na kaspazu 3 slabo izražena kod svih životinja. NaÅ”e istraživanje je pokazalo da hipotireoidizam u ranom postnatalnom periodu dovodi do promena u morfologiji i proliferaciji Äelija PE, bez efekta na uklanjanje Äelija delovanjem kaspaze 3. Ovaj model može da posluži u buduÄim istraživanjima potencijalne karcinogeneze u cilju prevencije i tretmana kancera jajnika
Generation of Au2Cl2n+1 (n = 1-3) cluster ions by laser desorption/ionization mass spectrometry
Gold chloride clusters were generated from an aqueous solution of gold-hydrochloric acid deposited onto the target plate using laser desorption/ionization (LDI) on a commercial matrix assisted laser desorption/ionization ātime of flight (MALDI-TOF) mass spectrometric instrument. In our experimental conditions negative mode of LDI mass spectra provided the most valuable informations. The mass peaks at m/z about 339, 499, 571, 641, were assigned to AuCl4-, Au2Cl3-, Au2Cl5-, Au2Cl7-cluster ions, respectively. The Au2Cl5-and Au2Cl7-clusters are detected for the first time in this work. This research showed that LDI MS, thanks to its easy experimental procedure, a soft ionization technique which may be a useful characterization tool in the chemistry of heterogeneous gold clusters.Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-30 September 2016
ISHRANA GRABLJIVIH VRSTA RIBA I NJIHOV UTICAJ NA ODRŽANJE STABILNOSTI AKUMULACIJE BOVAN
The aim of this study was to investigate the diet of adult predatory fish
(pikeperch, perch, pike, and catfish) that inhabit the Bovan reservoir and
determine their impact on the ecological stability of the reservoir. The results
showed that predators most often used planktivorous fish species in their diet,
including bleak, roach, and perch. These species are dominant prey to predators,
and at the same time important links in food chains. By feeding on planktivorous
fish, predatory fish can contribute to the reduction of the eutrophication of the
reservoir and keep this ecosystem stable.Publishe
Gender modulates development of the metabolic syndrome phenotype in fructose-fed rats
We analyzed the effects of a fructose-rich diet (FRD) to test the assumption that the expression of metabolic syndrome phenotype is different in male and female rats. Two-way ANOVA revealed a significant effect of FRD on feeding behavior and carbohydrate/lipid metabolism. The increased caloric intake in FRD rats of both sexes was followed by a cluster of gender-specific changes typical for the metabolic syndrome. Female rats were characterized by decreased glycemia, increased triglycerides, enlarged visceral adipose tissue and increased absolute mass of liver, without changes in systolic blood pressure and insulin sensitivity. In contrast, male rats developed less disturbances in physical and biochemical characteristics, but blood pressure and insulin sensitivity were impaired by FRD. The results emphasize the detrimental effects of fructose consumption on cardiovascular risk and insulin action in males, whereas females are affected by other metabolic disturbances. These results support the idea of gender-dependent differences in the expression of the metabolic syndrome phenotype.Projekat ministarstva br. 4100
Design of Experiments mathematical methodology applied in MALDI-TOF-MS analysis
U ovom radu, matematiÄka metoda Dizajn eksperimenta (eng. Design of Experiment, DoE) koriÅ”Äena je za optimizaciju instrumetalnih parametara masenog spektrometra sa laserskom desorpcijom i jonizacijom pomoÄu āmatriksaā i sa analizatorom na bazi vremena preleta (MALDI TOF MS), a za analizu metalnih kompleksa. Ulazni parametri DoE metodologije bili su intenzitet lasera, napon na āgriduā i broj impulsa lasera, dok su kao izlazni parametri od interesa bili odnos signal/Å”um i rezolucija. RazliÄite vrednosti ulaznih parametra (njihovi nivoi) definisane su na osnovu dobijenih preliminarnih eksperimenata. Nakon utvrÄivanja ulaznih i izlaznih parametara DoE eksperiment je izveden primenom Box-Behnken-ove metode. Teorijski predviÄene vrednosti za dobijanje kvalitetnog masenog spektra metalnog kompleksa (snaga lasera-1220 W, napon āgridaā-79 V i broj impulsa lasera-360) su eksperimentalno potvrÄeni.In this work Design of Experiment mathematical methodology (DoE) was used for optimization the instrumental parameters of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) for the analysis of metal complex. The input factors in DoE methodology were laser intensity, grid voltage and number of laser shots, while the output response of interest were signal-to-noise ratio and resolution. The different values of input factors (their level) are defined according to the results obtained in preliminary experiments. After the identification of input factors and responses of interest the DoE experimental plan is created using Box-Behnken methode. Theoretically predicted values for obtaining high-quality mass spectra of metal complex (laser power ā 1220 W, grid voltage ā 79 V and number of laser shots ā 360) are experimentally confirmed.53. Savetovanje Srpskog hemijskog druÅ”tva : Program i kratki izvodi radova : Kragujevac, Srbija, 29. i 30. maj 2015
The first observation of the presence of microplastics in wild common bleak (Alburnus alburnus L.) and standardization of extraction protocols
The presence of microplastics (MPs) in the gastrointestinal tract, muscle, and whole-body samples of common bleak Alburnus alburnus L. from Gruža Reservoir (Central Serbia) was studied for the first time. Different protocols for MPs extraction were applied to determine the most efficient one. The study aimed to modify existing protocols to be cost-effective, efficient in digestion, and with no detrimental effect on potentially present MPs polymers. In this study, the digestion with 10% KOH during 48 h at 40Ā°C was efficient for the gastrointestinal tract and muscle. Digestion with 10% KOH during 72 h at 40Ā°C was the most efficient for whole-body samples. The usage of NaClO proved successful in digestion of the gastrointestinal tract overnight at room temperature. Fibers detected in the samples are assumed to be of plastic origin. The general goal was to establish a protocol for extracting MPs from fish tissue in wild populations to obtain results and determine the degree of pollution.Publishe
Gender modulates development of the metabolic syndrome phenotype in fructose-fed rats
We analyzed the effects of a fructose-rich diet (FRD) to test the assumption that the expression of metabolic syndrome phenotype is different in male and female rats. Two-way ANOVA revealed a significant effect of FRD on feeding behavior and carbohydrate/lipid metabolism. The increased caloric intake in FRD rats of both sexes was followed by a cluster of gender-specific changes typical for the metabolic syndrome. Female rats were characterized by decreased glycemia, increased triglycerides, enlarged visceral adipose tissue and increased absolute mass of liver, without changes in systolic blood pressure and insulin sensitivity. In contrast, male rats developed less disturbances in physical and biochemical characteristics, but blood pressure and insulin sensitivity were impaired by FRD. The results emphasize the detrimental effects of fructose consumption on cardiovascular risk and insulin action in males, whereas females are affected by other metabolic disturbances. These results support the idea of gender-dependent differences in the expression of the metabolic syndrome phenotype.Projekat ministarstva br. 4100
KARDIOVASKULARNI FAKTORI RIZIKA FIZIÄKI AKTIVNIH STUDENTKINJA
Cardiovascular diseases are the leading cause of death in the world, and occur as a result of many risk factors (obesity, high blood pressure, inadequate physical activity, hyperlipoproteinemia, inadequate diet and an unhealthy lifestyle).Ā Given that in young adults most of these risk factors tend to remain at the same level or to even increase with age, especially after the second or third decade of life. This means that if young adults are in the at-risk group in terms of cardiovascular diseases, they usually stay in that group in later life. The research problem is to examine the strength and significance of possible linear correlations of certain cardiovascular risk factors in 53 physically active female university students (PE students), aged 19 to 25 years, as well as to examine possible differences between those with different lengths of sports experience (ranging from 0 to 5, 6 to 10 and 11 to 15 years). Their baseline characteristics (age, body height and mass, body mass index - BMI, resting metabolic rate - RMR, resting heart rate - RHR and length of sports experience) were determined, as well as the linear relationships of selected cardiovascular risk factors (body fat percentage - BF%, visceral fat level - Visc F, waist circumference - WC, arterial blood pressure - SBP and DBP). The data were analyzed (descriptive statistics, the Kolmogorov-Smirnov test, PeŠ°rsonās and Spearmanās correlation coefficient, ANOVA) using SPSS 21.0. The results of the analysis indicated a lack of statistically significant differences in cardiovascular risks factors between female PE students with different lengths of sports experience, and the existence of statistically significant (p<0.001), positive, mostly strong correlations, between most of the selected cardiovascular risk factors. When it comes to blood pressure parameters, although weak positive and statistically significant (p<0.05) correlations were established between SBP and BMI (r=.273), SBP and WC (r=.308), so as between DBP and body mass (r=.284), DBP and RHR (r=.287), DBP and RMR (r=.292), as well between DBP and WC (r=.304) and DBP and SBP (r=.571, p<0.001), it is unequivocal that the reduction of body mass at the expense of adipose tissue is necessary, as well as an increase in the moderate physical activity level and regularity, in order to reduce the risk of cardiovascular diseases in female PE students now as well as in older age.Kardiovaskularne bolesti vodeÄi su uzrok smrtnosti u svetu, a nastaju kao rezultat delovanja mnogobrojnih faktora rizika (gojaznost, visok krvni pritisak, nedovoljna fiziÄka aktivnost, hiperlipoproteinemija, neadekvatna ishrana i nezdrav stil života). S obzirom na to da veÄina ovih faktora rizika kod mladih odraslih ima tendenciju održavanja na istom nivou, pa Äak i pogorÅ”avanja sa godinama, posebno nakon druge, odnosno treÄe decenije života, to znaÄi da mladi odrasli, ako se nalaze u riziÄnoj grupi u pogledu kardiovaskularnih oboljenja, najÄeÅ”Äe ostaju u toj grupi i u kasnijem životnom dobu. Problem ovog istraživanja jeste ispitivanje postojanja moguÄih korelacija odreÄenih kardiovaskularnih faktora rizika 53 fiziÄki aktivnih studentkinja, uzrasta 19 do 25 godina, kao i da se ispitaju moguÄe razlike izmeÄu onih sa razliÄitom dužinom sportskog staža (u rasponu od 0 do 5, 6 do 10 i 11 do 15 godina). UtvrÄeni su njihovi opÅ”ti pokazatelji (uzrast, telesna visina, telesna masa, indeks telesne mase- BMI, bazalni metabolizam- RMR, frekvenca srca u miru- RHR i dužina sportskog staža), kao i relacije odabranih kardiovaskularnih faktora rizika (procenat masnog tkiva- BF%, nivo visceralnih masti- Visc F, obim struka- WC, arterijski krvni pritisak- SBP i DBP). Podaci su analizirani (deskriptivna statistika, Kolmogorov-Smirnov test, Pearson-ov i Spearman-ov koeficijent korelacije, ANOVA) primenom SPSS 21.0. Rezultati analize ukazali su na izostanak statistiÄki znaÄajnih razlika u kardiovaskularnim faktorima rizika izmeÄu studentkinja fakulteta sporta razliÄite dužine sportskog staža, ali i na postojanje statistiÄki znaÄajnih (p<0.001), pozitivnih korelacija, uglavnom velike jaÄine, veÄine odabranih kardiovaskularnih faktora rizika. Kada je reÄ o parametrima krvnog pritiska, iakoĀ male pozitivne i statistiÄki znaÄajne (p<0.05), ustanovljene su korelacije SBP sa BMI (r=.273), SBP sa WC (r=.308), kao i DBP sa telesnom masom (r=.284), DBP sa RHR (r=.287), DBP sa RMR (r=.292), kao i DBP sa WC (r=.304),ali i sa SBP (r=.571, p<0.001), nedvosmisleno je da je neophodna redukcija telesne mase na raÄun masnog tkiva, ali i poveÄanje nivoa i redovnosti umerene fiziÄke aktivnosti, kako bi se umanjio rizik od kardiovaskularnih bolesti studentkinja sportistkinja sada, ali i u starijim godinama
The importance of detailed hydrobiological research of rivers for the detection and conservation of originally preserved habitats
Due to multiple modern stressors, there are rapid changes in inland water habitats and
irreversible biodiversity loss. Hence, in order to detect parts of rivers or basins with
originally preserved habitat and biodiversity, detailed hydrobiological research of
inland waters (simultaneous research of basic abiotic parameters and hydrobiocenosis)
are necessary. The aim is to detect habitats' biological and ecological values, which are
important for the permanent preservation of genetic and species diversity and the
stability and functionality of the entire ecosystem or catchment area. In terms of
ecology and conservation, these habitats have the same importance for inland waters
and catchment areas as the hot-spot areas for preserving global biodiversity. The
importance of these areas in this work is presented on the example of the Veliki Rzav
River (Serbia). In order to support the program of declaring the river as a protected
area, the research was conducted in 2021. In the Veliki Rzav River, many biological
values such as preserved and high biodiversity of algae, macroinvertebrates, and fish
were detected. For the first time in Serbia, new species of algae were detected, along
with the significant presence of sensitive taxa in the macroinvertebrate community and
a stable brown trout population with the detection of the new haplotype
The importance of detailed hydrobiological research of rivers for the detection and conservation of originally preserved habitats
Due to multiple modern stressors, there are rapid changes in inland water habitats and
irreversible biodiversity loss. Hence, in order to detect parts of rivers or basins with
originally preserved habitat and biodiversity, detailed hydrobiological research of
inland waters (simultaneous research of basic abiotic parameters and hydrobiocenosis)
are necessary. The aim is to detect habitats' biological and ecological values, which are
important for the permanent preservation of genetic and species diversity and the
stability and functionality of the entire ecosystem or catchment area. In terms of
ecology and conservation, these habitats have the same importance for inland waters
and catchment areas as the hot-spot areas for preserving global biodiversity. The
importance of these areas in this work is presented on the example of the Veliki Rzav
River (Serbia). In order to support the program of declaring the river as a protected
area, the research was conducted in 2021. In the Veliki Rzav River, many biological
values such as preserved and high biodiversity of algae, macroinvertebrates, and fish
were detected. For the first time in Serbia, new species of algae were detected, along
with the significant presence of sensitive taxa in the macroinvertebrate community and
a stable brown trout population with the detection of the new haplotype