Efficiency of the using singulyar at treatment of the bronchial asthma beside children

Using singulyar for reinforcement base treatment beside children allows to reach checking on clinical symptoms of the bronchial asthma and vastly perfect the factors to functions of the external breathing at more short periods

Анкилозирующий спондилоартрит в ревматологической практике Карелии

The paper describes the pathological aspects of an inflammatory process in ankylosing spondyloarthritis (AS), the role of muscle spasm in maintaining the intensity of pain syndrome and stiffness, the need for the early diagnosis of AS, and the significance of the early use of nonster-oidal anti-inflammatory drugs in these patients. The results of clinical trials and the authorsX data demonstrate the high efficacy and good tolerance of nimesulide (nise) in AS.Представлены патоморфологические аспекты воспалительного процесса при анкилозирующем спондилоартрите (АС), роль мышечного спазма в поддержании интенсивности болевого синдрома и скованности, необходимость ранней диагностики АС, значение раннего применения нестероидных противовоспалительных препаратов у таких пациентов. Результаты клинических исследований и собственные данные авторов демонстрируют высокую эффективность и хорошую переносимость нимесулида (найз) при АС

Myeloid antigens in childhood lymphoblastic leukemia:clinical data point to regulation of CD66c distinct from other myeloid antigens

BACKGROUND: Aberrant expression of myeloid antigens (MyAgs) on acute lymphoblastic leukemia (ALL) cells is a well-documented phenomenon, although its regulating mechanisms are unclear. MyAgs in ALL are interpreted e.g. as hallmarks of early differentiation stage and/or lineage indecisiveness. Granulocytic marker CD66c – Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is aberrantly expressed on ALL with strong correlation to genotype (negative in TEL/AML1 and MLL/AF4, positive in BCR/ABL and hyperdiploid cases). METHODS: In a cohort of 365 consecutively diagnosed Czech B-precursor ALL patients, we analyze distribution of MyAg+ cases and mutual relationship among CD13, CD15, CD33, CD65 and CD66c. The most frequent MyAg (CD66c) is studied further regarding its stability from diagnosis to relapse, prognostic significance and regulation of surface expression. For the latter, flow cytometry, Western blot and quantitative RT-PCR on sorted cells is used. RESULTS: We show CD66c is expressed in 43% patients, which is more frequent than other MyAgs studied. In addition, CD66c expression negatively correlates with CD13 (p < 0.0001), CD33 (p = 0.002) and/or CD65 (p = 0.029). Our data show that different myeloid antigens often differ in biological importance, which may be obscured by combining them into "MyAg positive ALL". We show that unlike other MyAgs, CD66c expression is not shifted from the onset of ALL to relapse (n = 39, time to relapse 0.3–5.3 years). Although opposite has previously been suggested, we show that CEACAM6 transcription is invariably followed by surface expression (by quantitative RT-PCR on sorted cells) and that malignant cells containing CD66c in cytoplasm without surface expression are not found by flow cytometry nor by Western blot in vivo. We report no prognostic significance of CD66c, globally or separately in genotype subsets of B-precursor ALL, nor an association with known risk factors (n = 254). CONCLUSION: In contrast to general notion we show that different MyAgs in lymphoblastic leukemia represent different biological circumstances. We chose the most frequent and tightly genotype-associated MyAg CD66c to show its stabile expression in patients from diagnosis to relapse, which differs from what is known on the other MyAgs. Surface expression of CD66c is regulated at the gene transcription level, in contrast to previous reports

A Novel Ecdysone Receptor Mediates Steroid-Regulated Developmental Events during the Mid-Third Instar of Drosophila

The larval salivary gland of Drosophila melanogaster synthesizes and secretes glue glycoproteins that cement developing animals to a solid surface during metamorphosis. The steroid hormone 20-hydroxyecdysone (20E) is an essential signaling molecule that modulates most of the physiological functions of the larval gland. At the end of larval development, it is known that 20E—signaling through a nuclear receptor heterodimer consisting of EcR and USP—induces the early and late puffing cascade of the polytene chromosomes and causes the exocytosis of stored glue granules into the lumen of the gland. It has also been reported that an earlier pulse of hormone induces the temporally and spatially specific transcriptional activation of the glue genes; however, the receptor responsible for triggering this response has not been characterized. Here we show that the coordinated expression of the glue genes midway through the third instar is mediated by 20E acting to induce genes of the Broad Complex (BRC) through a receptor that is not an EcR/USP heterodimer. This result is novel because it demonstrates for the first time that at least some 20E-mediated, mid-larval, developmental responses are controlled by an uncharacterized receptor that does not contain an RXR-like component

The concept of creating a modern terminal and warehouse complex as a tool for the development of transport and logistics infrastructure

Структура магистерской диссертации включает в себя введение, три главы, заключение, список использованных источников и приложения. Во введении обоснован выбор темы исследования, ее актуальность, сформулированы цель и задачи работы, определены предмет и объект исследования, его теоретические и методологические основы, источники получения информации, сформулирована научная новизна. В первой главе рассмотрены подходы ученых к определению понятия девелопмента, выделены виды девелопмента и особенности его функционирования. Во второй главе проанализирован рынок складской недвижимости. Представлена классификация складской недвижимости. А также выявлены основные проблемы транспортно-логистической инфраструктуры региона. В третьей главе подробно описан проект создания терминально-складского комплекса. Описаны характеристики планируемой застройки, этапы реализации проекта и проведен анализ финансовых результатов проекта. В заключении сформулированы основные выводы и обобщены результаты исследования.Structure of master's thesis includes introduction, three chapters, conclusion, list of used sources and applications. In the introduction the choice of the research topic, its topicality, formulates the goal and objectives, defined the subject and object of research, its theoretical and methodological foundations, sources of information, formulated the scientific novelty. In the first Chapter the approaches of scientists to definition of the concept of development, highlighted the types of development and peculiarities of its functioning. The second Chapter analyzes the market of warehouse real estate. The classification of warehouses. As well as the basic problems of transport and logistics infrastructure of the region. The third Chapter describes in detail the project of creation of terminal and warehouse complex. Describes the characteristics of the proposed development, the stages of the project and the analysis of the financial results of the project. In conclusion the main conclusions and summarizes the results of the stud

Insulin, insulin resistance and anthropometric parameters in overweight and obese women

Insulin resistance together with obesity, hypertension and dyslipidemia is part of the cluster that represents the metabolic syndrome. The impaired insulin sensitivity has been reported to be linked with obesity; however the importance of the fat distribution has also been emphasized in the development of this disorder. The aim of our study was to determine to association of serum insulin and insulin resistance assessed by HOMA with anthropometric parameters in obese and overweight women. Also the correlation of insulin and insulin resistance (HOMA-IR) with the subcutaneous and visceral abdominal fat was evaluated. The study included 41 overweight and obese women, aged 20- 60 years. The anthropometric parameters like body mass index (BMI), waist, hip and thigh circumference were measured with standard methods. Abdominal subcutaneous fat tissue (SFT) and visceral fat tissue (VFT) were determined by ultrasonography. Insulin values were measured with radioimmunoassay and the insulin resistance was assessed by the HOMA2 method. The results of our study showed strong correlation of insulin and HOMA-IR with BMI (r 0.36, p<0.01). The median and mean values of these parameters were much higher in subjects with obesity grade 2 and 3. The insulin concentrations and HOMA-IR also showed good correlation with waist and hip circumference and with the subcutaneous fat tissue (p<0.05). The results of our study point to the fact that in addition to the total body fat accumulation, the role of the subcutaneous fat tissue in the development of insulin resistance in overweight and obese Macedonian women should not be underestimated. Key words: insulin resistance, subcutaneous fat, visceral fa

Correlation of serum leptin with anthropometric parameters and abdominal fat depots determined by ultrasonography in overweight and obese women

Leptin is an adipocytokine that has an important role in energy homeostasis and therefore in the pathophysiology of obesity. Leptin levels are influenced by many factors such as gender, race, energy intake, fat mass, etc. The aim of our study is to investigate the relationship of circulating leptin levels to anthropometric parameters and to the subcutaneous and visceral fat tissue determined by ultrasonography in healthy overweight and obese women. The study included 50 healthy women, with body mass index (BMI) above 25 kg/m2. Measurements of anthropometric parameters were performed with a standardized technique. The subcutaneous and visceral fat tissue was measured with ultrasound 1 cm above the umbilicus. Leptin was determined by radioimmunoassay. The serum leptin levels presented a positive correlation between the levels of leptin and BMI (r-0.73, p < 0.05) waist circumference (r-0.73, p < 0.05) hip circumference (r-0.74, p < 0.05), thigh circumference (r-0.56, p < 0.05) and abdominal subcutaneous fat tissue (r- 0.46, p < 0.05). There was no correlation between leptin and visceral fat tissue, waist to hip and waist to thigh ratio. The results of our study confirmed the correlation between leptin and BMI. The correlation between leptin and all measured circumferences cannot be attributed to a particular fat depot, but rather reflect the correlation between this adipocytokine and the total body fat. The subcutaneous fat depot determined by ultrasound showed a correlation with leptin, while this kind of connection was not established for the visceral fat tissue. Key words: Leptin, abdominal subcutaneous fat, abdominal visceral fat

Ankylosing spondyloarthritis in the rheumatology practice of Karelia

The paper describes the pathological aspects of an inflammatory process in ankylosing spondyloarthritis (AS), the role of muscle spasm in maintaining the intensity of pain syndrome and stiffness, the need for the early diagnosis of AS, and the significance of the early use of nonster-oidal anti-inflammatory drugs in these patients. The results of clinical trials and the authorsX data demonstrate the high efficacy and good tolerance of nimesulide (nise) in AS

Determining specific thyroid transcripts in peripheral blood: A single center study experience

Thyroid carcinoma (TC) comprises a spectrum of different tumors with a wide range of biological behavior and prognosis. The techniques based on the latest trends in molecular biology may have application in diagnosis of metastatic TC. The aim of this study was to apply and analyze mRNA expression in peripheral blood of thyrotropin receptor [thyroid stimulating hormone receptor (TSHR-mRNA)] gene and thyroglobulin (Tg-mRNA) gene using 2–ΔΔCt method in differentiated TC patients and healthy individuals. Fifty-seven subjects were included in the study, consisting of 40 patients with TC and 17 healthy volunteers as a control group. Total RNA was isolated from peripheral blood and used for two-step reverse transcriptase-polymerase chain reaction (PCR). Real-time PCR was performed with appropriate primers. Relative quantification using the 2–ΔΔCt method was applied. Thyroid carcinoma patients with metastatic disease or loco-regional relapse expressed TSHR-mRNA by a 8.57-fold higher level than healthy controls. Thyroid carcinoma patients with biochemical relapse expressed TSHR-mRNA by a 14.17-fold higher level than healthy controls, while expression of Tg-mRNA was 6.6-fold higher in TC patients with metastatic disease and loco-regional relapse than healthy controls and 8.34-fold higher level compared with TC patients with excellent response to treatment. Our preliminary study showed that the TSHR gene expression might have more useful application as a biomarker compared to detection of Tg gene expression