The format of training family doctors on palliative care in the Dnipropetrovsk region.

The first global palliative care resolution WHA 67.19 has been developed recently, in 2014, where the need to improve access to palliative care around the world is first declared. In the article, the authors analyzed the change trends and the state of the medical care delivery system for incurable patients in the world and Ukraine. As the civilization of the state and the humanity of society as a whole is assessed by the level of PHC provision in today's world, this problem has attracted the attention of central (Ukraine) and regional (Dnipro city) government bodies, which was reflected in the search for new medical and educational initiatives. The need of the time is the need to focus the attention of family doctors on the issue that palliative care is a qualification component of their daily work, which will help to remove incurable patients from the periphery of attention of their multicomponent work, and will also help to clearly build the interaction between the primary and secondary link, in particular, with hospice departments of specialized hospitals. In particular, the experience of implementing a joint project of a new format for training family doctors on palliative care issues, realized by the Department of Family Medicine of the SE “DMA” and the Municipal non-commercial enterprise "City Clinical Hospital No. 2” of Dnipro City Council is described. In­novative forms of conducting classes help to expand the boundaries of the possibilities of teaching problematic issues of palliative medicine, which account for only 0.6% of instructional time in the existing program of training family doctors of secondary specialization, and the multicomponent format for studying palliative care issues, implemented, inter alia, with support of Director of the Municipal non-commercial enterprise "City Clinical Hospital No. 2” of Dnipro City Council (visiting hospice and palliative departments delivering secondary level of medical care assistance, a structured regulatory unit for the provision of PHC in Ukraine, working out of patients’ routing issues, interactive teaching methods, etc.) leaves in the memory of physician-trainees not only the essential content, but also a deep emotional trace, which helps to increase the motivation of the physician trainees, improves coordination between primary and secondary palliative care and improves the quality of training

Study of the functional product’s protein compounds digestion features

The aim of the study was to investigate the transformation of meat product’s proteins from pig hearts and aortas during enzymatic hydrolysis in an in vitro model of the gastrointestinal tract. The model consisted of three phases simulating digestion processes: “oral cavity” phase (a-amylase, pH 7.0; 2 min), “stomach” phase (pork pepsin, pH 3.0; 120 min), “intestine” phase (pork pancreatin, pH 7.0; 130 min). The product was sequentially subjected to hydrolysis, at the end of each phase, samples were taken to determine the protein concentration (biuret method) and visualize the protein fractions (one-dimensional electrophoresis). A significant increase in protein concentration at the “stomach” phase was revealed by 3.2 times, and the absolute content by 4.6 times. At the “intestine” phase, a decrease in the number of peptide complexes with copper ions by 1.8 times, the absolute protein content by 8.5% was re‑ vealed. The noted tendency was confirmed by electrophoretic studies — at the stage, simulating digestion in the stomach, the prod‑ ucts of meat product’s proteins hydrolysis were visualized; at the “intestine” phase, a low expression of protein fractions in the range of more than 10 kDa is shown. The maximum hydrolysis of protein compounds at the “stomach” phase to poly- and oligopeptides was confirmed, continuing at the “intestine” stage with the accumulation of free amino acids. This methodology makes it possible to visualize the products of hydrolysis of proteins in a meat product at all stages of the model and to monitor changes in protein concentration in the system

Evolution of in vitro digestibility techniques: a systematic review

The inability to reproduce certain digestive processes in vivo, high research costs and ethical aspects have led to the development of a large number of in vitro digestion models. These models allow us to take into account various factors of modeling complex multistage physiological processes occurring in the gastrointestinal tract, which makes them promising and widely used. A significant part of in vitro methods includes assessment by enzymatic digestion and are based on the calculation of nitrogen remaining after digestion in relation to the initial total nitrogen (according to the Dumas, Kjeldahl method, spectrophotometric or chromatographic method). There are also a number of titrometric methods (pH‑stat), which are mainly used to assess the digestibility of feed, most successfully for aquatic animals due to the simplicity of their digestive tract. Methods for assessing the digestibility of food products by enzymatic digestion have undergone various stages of evolution (since 1947) and have been widely modified by including various enzymes (pepsin, trypsin, pancreatin, erepsin, etc.) in model systems, indices for various products have been determined on their basis (pepsin-digest-residue (PDR) index, 1956; pepsin pancreatin digest (PPD) index, 1964; pepsin digest dialysate (PDD), 1989). As a result, a single protocol was formed to study the digestibility of food — INFOGEST (2014–2019), which includes three stages of digestion (oral, gastric and intestinal). It allows researchers to accurately reproduce the conditions of the human gastrointestinal tract and is widely used by scientists around the world

Новые публикации по судебной экспертизе

This section presents translated abstracts of selected papers that appeared in the following periodicals: Journal of Forensic Sciences [www.wileyonlinelibrary.com/journal/jfo] and Forensic Science International: Animals and Environments [www.elsevier.com/locate/fsiaе].Представлены переводы рефератов избранных статей, опубликованных в зарубежных периодических изданиях: Journal of Forensic Sciences [www.wileyonlinelibrary.com/journal/jfo] и Forensic Science International: Animals and Environments [www.elsevier.com/locate/fsiaе]

Comparative estimation of biochemical composition of fruits of Ericaceae species under conditions of Belarus

Thus, as a result of biochemical screening of 30 taxons of Ericaceae species taking into account 32 parameters in a long-term cycle of observation it has been established the different degree of dependence of variability level of biochemical structure components of fruits of alien crops on genotype and a hydrothermal mode of the period of their maturing has been revealed. The similarity of parameters of genotypic variability of some traits of all investigated Ericaceae species is also revealed: low one – for the general contents in fruits of soluble sugars, flavonols, of potassium, calcium, magnesium and high one – for the contents of anthocyanins, and also the ratio of fractions of pectinaceous substances and bioflavonoids. Specific features of genetic determinacy of the analyzed traits, testifying the greatest degree of its displays of V. corymbosum for total accumulation of soluble sugars and bioflavonoids, flavonols contents, calcium and magnesium and by the lowest degree – for the contents of titratable acids, vitamin C, anthocyanins and values of a sugar-acid index were established. If to mention V. vitis-idaea L., the parameters of general accumulation in fruits of soluble sugars, dry substances and all major mineral elements were characterized by the greatest degree of genetic determinacy, whereas by the least degree – the contents of anthocyanins, catechines and tannins. If to mention V. macrocarpon, the parameters of accumulation in fruits of dry substances, nitrogen, potassium, calcium, phenol-carboxylic acids have been noted by the most expressed genetic determinacy, and by the least expressed – the contents of anthocyanins, sucrose and pectinaceous substances in fruits

Genotypic distinctions of variability of biochemical composition of fruits of Vacciniaceae species under conditions of Belarus

Result of research of quantitative characteristics of biochemical composition of fruits of 30 taxons of 3 Vacciniaceae species (such as V. corymbosum L. (highbush blueberry), V. vitis-idaea L. (lingonberry) and Oxycoccus macrocarpus (Ait.) Pers. (cranberry)) inter-specific distinctions of a degree of stability of its separate components to complex influence of meteorological factors are revealed by 32 parameters (traits) describing the contents in fruits of some organic acids, carbohydrates, phenolic compounds, terpenoids and major mineral elements are presented. The cultivars possessing by the greatest and accordingly by the least levels of dependence on abiotic factors are identified

Assessment of the role of the state in the management of mineral resources

Mineral resources as natural capital can be transformed into human, social and physical capital, that guarantees the sustainable development of a country, exclusively through professional public management. Public management of a country's mineral resource potential is seen as an element of transnational governance. Regulation involves the use of laws, rules and regulations within the jurisdictional and sectoral capabilities of the state, minimizing its involvement as a producer. The features of the ideology of economic liberalism, which polarizes the societies of raw material producing countries by imposing austerity, as well as lowering trade barriers have been studied. The analysis of the influence of the radical new order of neoliberal world ideology on the development of the extractive sector and the strengthening of state regulation has been presented

Применение метода тонкослойной хроматографии для анализа антиоксидантной активности

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.Для исследования антиоксидантной активности растительного сырья, которое является богатым источником эффективных нетоксичных биологически активных веществ, используются различные физикохимические методы анализа. В данной работе был применен метод высокоэффективной тонкослойной хроматографии (ВЭТСХ) для разделения биологически активных веществ, входящих в этанольные экстракты шелухи красного, желтого и белого лука, сушенных розмарина, базилика и чаги. Антиоксидантную активность полученных фракций на хроматографической пластине оценивали последующим DPPH-скринингом. Наибольшими показателями антиоксидантной активности и вариабельностью качественного состава характеризовались экстракты шелухи красного и желтого лука, а также розмарина, причем для этих образцов была отмечена схожесть антиоксидантных профилей. В экстракте белого лука не было обнаружено каких-либо классов антиоксидантов. Для шелухи красного лука было отменено наличие значительно выраженных пятен по всему треку, значения Rf которых расположены в диапазоне 0,13-0,97. Доказано, что все экстракты, кроме шелухи белого лука и чаги, в разной степени интенсивности имеют пятна с коэффициентом замедления в диапазоне 0,96-0,98, что соответствует полученному значению Rf кверцетина