Mulher e testemunho: das (im)possibilidades de lembrar, esquecer e dizer

Este trabalho tem como objetivo problematizar o estatuto do testemunho como discurso político que (re)clama a urgência de uma escuta pública para restituir um espaço de memória e de dizer, justamente onde a linguagem e o sujeito foram violentamente interditados. Estabelecemos um diálogo teórico entre a Análise do Discurso, a Teoria Literária e os Estudos de Gênero. O corpus é constituído por testemunhos de prisioneiras e ex-prisioneiras políticas, que participaram como combatentes e militantes do Partido Comunista do Peru - Sendero Luminoso, na guerra interna que viveu o país. As análises demonstram como estas mulheres conseguiram elaborar suas perdas, durante os anos de confinamento, a través de processos de subjetivação y práticas de resistência realizadas em “trincheira” (prisão), sustentadas por relações de frátria. Ao testemunharem se inscrevem na história, reivindicam uma memória e enfrentam o desafio de lembrar, esquecer e dizer o que é (im)possível, com a expectativa de romper com o silêncio que lhes foi imposto.PALAVRAS-CHAVE: Sendero, Discurso, Feminismo

Reflexões sobre a preparação e o acompanhamento de famílias na adoção.

This work presents and discusses the perspectives of preparation and monitoring of families interested in adoption, based on the changes proposed by the new legislation guidelines (Law 8.069 / 1990 and Law 12.10 / 2009), which establish the need for psychosocial preparation for the qualification for adoption. Different perspectives on this process of preparation for adoption are presented here, with an emphasis on the proposals developed by the Adoption Support Groups.Este trabajo presenta y discute las perspectivas de preparación y monitoreo de las familias interesadas en la adopción, con base en los cambios propuestos por las nuevas directrices legislativas (Ley 8.069 / 1990 y Ley 12.10 / 2009), que establecen la necesidad de preparación psicosocial para el calificación para la adopción. Aquí se presentan diferentes perspectivas sobre este proceso de preparación para la adopción, con énfasis en las propuestas desarrolladas por los Grupos de Apoyo a la Adopción.Esse trabalho apresenta e discute as perspectivas da preparação e do acompanhamento de famílias interessadas na adoção, a partir das mudanças propostas pelas novas diretrizes da legislação (Lei n. 8.069/1990 e Lei 12.10/2009), que estabelecem a necessidade de uma preparação psicossocial para a habilitação para a adoção. São apresentadas aqui diferentes perspectivas sobre esse processo de preparação para a adoção, com ênfase nas propostas desenvolvidas pelos Grupos de Apoio à Adoção

Coexistence of chronic cutaneous lupus erythematosus and frontal fibrosing alopecia

Lupus erythemathosus is a chronic, relapsing disease with acute, subacute, and chronic lesions. Effluvium telogen occurs in the setting of systemic activity of the disease, and cicatricial alopecia results from discoid lesionsin on the scalp. Other types of alopecia, like alopecia areata, may rarely be found in lupus. Frontal fibrosing alopecia is characterized by frontotemporal hairline recession and eybrow loss. Histophatologically, it cannot be differentiated from lichen planopilaris. It is controversial whether frontal fibrosing alopecia is a subtype of lichen planopilaris.. A pacient with chronic lichenoid lupus erythematosus is described with clinical, histophatological and dermoscopic features of frontal fibrosing alopecia. We have not been able to find in the literature cases of frontal fibrosing alopecia as a clinical manifestation of lupus.Univ Fed Sao Paulo, Unifesp, Connect Tissue Dis Outpatient Clin, Dept Dermatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Unifesp, Dept Dermatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Unifesp, Dept Anat Pathol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Unifesp, Connect Tissue Dis Outpatient Clin, Dept Dermatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Unifesp, Dept Dermatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Unifesp, Dept Anat Pathol, Sao Paulo, SP, BrazilWeb of Scienc

Proteome Analysis of Leptospira interrogans Virulent Strain

Leptospirosis is a worldwide zoonotic infection of human and veterinary concern. Caused by pathogenic spirochetes of the genus Leptospira, the disease presents greater incidence in tropical and subtropical regions. The identification of proteins that could be involved in the bacteria host interactions may facilitate the search for immune protective antigens. We report the proteomic analysis of Leptospira interrogans serovar Pomona virulent strain LPF cultured from kidney and liver of infected hamsters. Total protein extracts were separated by two-dimensional gel electrophoresis (2-DE), 895 spots were analyzed by MALDI-TOF mass spectrometry (MS), and 286 were identified as leptospiral proteins, corresponding to 108 distinct proteins. These proteins are allocated in all the bacterial cell compartments and are distributed in every functional category. Furthermore, the previously described, known outer membrane proteins, OmpL1, LipL21, LipL31, LipL32/Hap-1, LipL41, LipL45, LipL46, LruA/LipL71, and OmpA-like protein Loa22 were all recognized. Most importantly, this research work identified 27 novel leptospiral proteins annotated as hypothetical open reading frames (ORFs). We report for the first time an array of proteins of the Leptospira expressed by virulent, low-passage strain. We believe that our studies, together with the genome data will enlighten our understanding of the disease

O ENSINO DE GEOMETRIA NAS SÉRIES INICIAIS DO ENSINO FUNDAMENTAL: ANALISANDO AS CONCEPÇÕES DOS ACADÊMICOS DO NORMAL SUPERIOR

O presente trabalho relata os resultados de um estudo que investigou concepções e dificuldades de acadêmicos do Curso Normal Superior da Universidade Católica Dom Bosco/Campo Grande-MS a respeito do ensino de Geometria nas séries iniciais do ensino fundamental. Os dados foram coletados mediante observações e aplicação de um questionário durante a realização de um minicurso que envolvia atividades relacionadas às formas tridimensionais e bidimensionais. O questionário tinha o propósito de identificar as concepções e dificuldades dos participantes a respeito da diferenciação entre sólidos e figuras planas. Os dados revelaram que as dificuldades dos acadêmicos, em Geometria, estão relacionadas tanto à nomeação e representação no plano de formas tridimensionais, como à diferenciação entre sólidos e figuras planas. Quanto às concepções, os acadêmicos apontam o livro didático como uma das principais alternativas para conduzir o ensino de Geometria.

Psychometric evaluation of the SF-36 (v.2) questionnaire in a probability sample of Brazilian households: results of the survey Pesquisa Dimensões Sociais das Desigualdades (PDSD), Brazil, 2008

<p>Abstract</p> <p>Background</p> <p>In Brazil, despite the growing use of SF-36 in different research environments, most of the psychometric evaluation of the translated questionnaire was from studies with samples of patients. The purpose of this paper is to examine if the Brazilian version of SF-36 satisfies scaling assumptions, reliability and validity required for valid interpretation of the SF-36 summated ratings scales in the general population.</p> <p>Methods</p> <p>12,423 individuals and their spouses living in 8,048 households were selected from a stratified sample of all permanent households along the country to be interviewed using the Brazilian SF-36 (version 2). Psychometric tests were performed to evaluate the scaling assumptions based on IQOLA methodology.</p> <p>Results</p> <p>Data quality was satisfactory with questionnaire completion rate of 100%. The ordering of the item means within scales clustered as hypothesized. All item-scale correlations exceeded the suggested criteria for reliability with success rate of 100% and low floor and ceiling effects. All scales reached the criteria for group comparison and factor analysis identified two principal components that jointly accounted for 67.5% of the total variance. Role emotional and vitality were strongly correlated with physical and mental components, respectively, while social functioning was moderately correlated with both components. Role physical and mental health scales were, respectively, the most valid measures of the physical and mental health component. In the comparisons between groups that differed by the presence or absence of depression, subjects who reported having the disease had lower mean scores in all scales and mental health scale discriminated best between the two groups. Among those healthy and with one, two or three and more chronic illness, the average scores were inverted related to the number of diseases. Body pain, general health and vitality were the most discriminating scales between healthy and diseased groups. Higher scores were associated with individuals of male sex, age below 40 years old and high schooling.</p> <p>Conclusions</p> <p>The Brazilian version of SF-36 performed well and the findings suggested that it is a reliable and valid measure of health related quality of life among the general population as well as a promising measure for research on health inequalities in Brazil.</p

Biologic therapy-induced pemphigus

Univ Fed Sao Paulo Unifesp, Dept Dermatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, Dept Pathol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, Dept Dermatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, Dept Pathol, Sao Paulo, SP, BrazilWeb of Scienc

A novel leptospiral protein increases ICAM-1 and E-selectin expression in human umbilical vein endothelial cells

It has been reported previously that activation of vascular endothelium by outer membrane proteins of the spirochetes Borrelia sp. and Treponema sp. resulted in enhanced expression of endothelial cell adhesion molecules. To investigate the role of leptospiral proteins in this process, a predicted lipoprotein encoded by the gene LIC10365 was selected, which belongs to a paralogous family that presents a domain of unknown function, DUF1565. The LIC10365 gene was cloned and the protein expressed in Escherichia coli C43 (DE3) strain using the vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and was used to assess its ability to activate cultured human umbilical vein endothelial cells. The rLIC10365 activated endothelium in such a manner that E-selectin and intercellular adhesion molecule 1 (ICAM-1) became upregulated in a dose-dependent fashion. The LIC10365-encoded protein was identified in vivo in the renal tubules of animal during experimental infection with Leptospira interrogans. Collectively, these results implicate the LIC10365-coding protein of L. interrogans as a potential effector molecule in the promotion of a host inflammatory response. This is the first report of a leptospiral protein capable of up-regulating the expression of endothelial cell adhesion molecules ICAM-1 and E-selectin.Instituto de Biotecnologia y Biologia Molecula

A novel leptospiral protein increases ICAM-1 and E-selectin expression in human umbilical vein endothelial cells

It has been reported previously that activation of vascular endothelium by outer membrane proteins of the spirochetes Borrelia sp. and Treponema sp. resulted in enhanced expression of endothelial cell adhesion molecules. To investigate the role of leptospiral proteins in this process, a predicted lipoprotein encoded by the gene LIC10365 was selected, which belongs to a paralogous family that presents a domain of unknown function, DUF1565. The LIC10365 gene was cloned and the protein expressed in Escherichia coli C43 (DE3) strain using the vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and was used to assess its ability to activate cultured human umbilical vein endothelial cells. The rLIC10365 activated endothelium in such a manner that E-selectin and intercellular adhesion molecule 1 (ICAM-1) became upregulated in a dose-dependent fashion. The LIC10365-encoded protein was identified in vivo in the renal tubules of animal during experimental infection with Leptospira interrogans. Collectively, these results implicate the LIC10365-coding protein of L. interrogans as a potential effector molecule in the promotion of a host inflammatory response. This is the first report of a leptospiral protein capable of up-regulating the expression of endothelial cell adhesion molecules ICAM-1 and E-selectin.Instituto de Biotecnologia y Biologia Molecula