Controle da ferrugem do cafeeiro (Hemileia vastatrix) com Bacillus subtilis: folhas destacadas e mudas.

O efeito dos isolados AP-3 e AP-150 de B. subtilis aplicados em suspensoes autoclavadas ou nao, foi avaliado sobre folhas destacadas e mudas de cafeeiro da variedade caturra, no controle da ferrugem. As pulverizacoes das suspensoes de B. subtilis foram realizadas 72 horas antes da inoculacao do patogeno, ambas com auxilio de pistola acoplada a bomba de vacuo, sobre a superficie inferior das folhas. Apos inoculacao do patogeno as folhas e mudas permaneceram em camara umida e escura a +- 24 C. Trancorrido deste periodo, a incubacao das folhas foi a +- 25 C com fotoperiodo de 12 horas, enquanto das mudas em casa de vegetacao a +- 25 C. Mesmo utilizando uma variedade extremamente susceptivel e uma raca de H. vastatrix altamente agressiva foi verificado, tanto emfolhas destacadas quanto em mudas, que B. subtilis controlou a ferrugem quando avaliado o numero de lesoes/folha. Para os dois isolados do antagonista a suspensao nao autoclavada foi mais eficiente que a autoclavada. Outro fato interessante e que os tratamentos com suspensao nao autoclavados de B. subtilis apresentavam periodo latente superior aos demais tratamentos. Os dois isolados de B. subtilis apresentaram comportamento semelhante

The Physiological Race of the first Hemileia vastatrix (Coffee Leaf Rust) discovered in Hawaii

29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio

A chromosome-level genome resource for studying virulence mechanisms and evolution of the coffee rust pathogen Hemileia vastatrix

29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio

First report of the physiological race (XXIV) of Hemileia vastatrix (Coffee Leaf Rust) in Hawaii

This research was funded by the Foundation for Science and Technology (FCT) UNIT (UID/AGR/04129/2020) of LEAF- -Linking Landscape, Environment, Agriculture and Food, Research Unit.info:eu-repo/semantics/publishedVersio

A chromosome-level genome resource for studying virulence mechanisms and evolution of the coffee rust pathogen Hemileia vastatrix

Recurrent epidemics of coffee leaf rust, caused by the fungal pathogen Hemileia vastatrix, have constrained the sustainable production of Arabica coffee for over 150 years. The ability of H. vastatrix to overcome resistance in coffee cultivars and evolve new races is inexplicable for a pathogen that supposedly only utilizes clonal reproduction. Understanding the evolutionary complexity between H. vastatrix and its only known host, including determining how the pathogen evolves virulence so rapidly is crucial for disease management. Achieving such goals relies on the availability of a comprehensive and high-quality genome reference assembly. To date, two reference genomes have been assembled and published for H. vastatrix that, while useful, remain fragmented and do not represent chromosomal scaffolds. Here, we present a complete scaffolded pseudochromosome-level genome resource for H. vastatrix strain 178a (Hv178a). Our initial assembly revealed an unusually high degree of gene duplication (over 50% BUSCO basidiomycota_odb10 genes). Upon inspection, this was predominantly due to a single scaffold that itself showed 91.9% BUSCO Completeness. Taxonomic analysis of predicted BUSCO genes placed this scaffold in Exobasidiomycetes and suggests it is a distinct genome, which we have named Hv178a associated fungal genome (Hv178a AFG). The high depth of coverage and close association with Hv178a raises the prospect of symbiosis, although we cannot completely rule out contamination at this time. The main Ca. 546 Mbp Hv178a genome was primarily (97.7%) localised to 11 pseudochromosomes (51.5 Mb N50), building the foundation for future advanced studies of genome structure and organization.info:eu-repo/semantics/publishedVersio

First report of a genome sequence resource of Colletotrichum kahawae, the causal agent of coffee berry disease

29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio

Potential association of HCF164, a chloroplast nuclear-encoded thioredoxin-like protein, with Coffea SH9 resistance factor against Hemileia vastatrix

29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio

Legitimacy and implications of reducing Colletotrichum kahawae to subspecies in plant pathology

info:eu-repo/semantics/publishedVersio

Identification of Colletotrichum species associated with anthracnose disease of coffee in Vietnam

Colletotrichum gloeosporioides, C. acutatum, C. capsici and C. boninense associated with anthracnose disease on coffee (Coffea spp.) in Vietnam were identified based on morphology and DNA analysis. Phylogenetic analysis of DNA sequences from the internal transcribed spacer region of nuclear rDNA and a portion of mitochondrial small subunit rRNA were concordant and allowed good separation of the taxa. We found several Colletotrichum isolates of unknown species and their taxonomic position remains unresolved. The majority of Vietnamese isolates belonged to C. gloeosporioides and they grouped together with the coffee berry disease (CBD) fungus, C. kahawae. However, C. kahawae could be distinguished from the Vietnamese C. gloeosporioides isolates based on ammonium tartrate utilization, growth rate and pathogenictity. C. gloeosporioides isolates were more pathogenic on detached green berries than isolates of the other species, i.e. C. acutatum, C capsici and C. boninense. Some of the C. gloeosporioides isolates produced slightly sunken lesion on green berries resembling CBD symptoms but it did not destroy the bean. We did not find any evidence of the presence of C. kahawae in Vietnam