An animal study - underutilized vista of research in dentistry with special reference to biocompatibility of root canal sealer

Background: Endodontic sealers are designed to be used only within the root canal but are frequently extruded through the apical constriction and often placed in intimate contact with periapical tissues for extended periods of time. Hence, assessment of biocompatibility of endodontic sealers is critical to the clinical success of endodontic therapy. Materials and Methods: Eighteen Wistar rats were divided into three groups of 6 each for observation after completion of 14, 30 and 90 days following implantation, respectively. Polyethylene tubes filled with new sealer, and tube without sealer [control] were implanted subcutaneously. The sample subcutaneous tissues from sacrificed rats were analyzed histologically for inflammatory response and were graded with FDI criteria as minimal, moderate and severe. Results were analyzed statistically with Student′s t-test and ANOVA tests. Results : Inflammatory reaction to the polyethylene tube was minimal at 14 and 90 days period and to the new sealer it was severe at 14 days and moderate at 30 and 90 days period. Conclusions: 1. Cytotoxicity of the individual ingredient of the new sealer should be investigated to find out its chemical reaction occurring at tissue interface resulting in persistence of inflammation. 2. This subcutaneous implantation method is a practical method for qualitative evaluation of endodontic material and can yield exact detailed information about tissue reaction of material on a cellular level. 3. Hence, animal study is positive, efficient and valuable method to carry out research successfully in dentistry

Photodynamic therapy for endodontic treatment of primary teeth: Original research article

Background: Tooth decay and traumatic dental injuries are the main causes of pulp inflammation in primary teeth. The present study was conducted on photodynamic therapy for endodontic treatment of primary teeth. Materials & Methods: 40 primary anterior teeth with pulp necrosis were divided into 2 groups: Group I, conventional root canal therapy and group II, conventional root canal therapy combined with antimicrobial PDT. Each group with 20 patients. Microbiological samples of the intra-canal content were taken using paper cones. Results: Group I & II had 19 boys and 9 girls and 8 boys and 12 girls respectively. Colony-forming units (CFU/mL) before and after treatment in group I at sample 1, 2, 3, 4, 5, 6, 7, 8, 9& 10 were 94000 and 0, 87040 and 2433, 14530 and 0,154231 and 4500, 123145 and 3200, 28453 and 550, 110232 and 1250,112456 and 3100, 43294 and 0 and 142945 and 1955 respectively. CFU before and after treatment in group II at sample 1,2,3,4,5,6,7,8,9 &10 were 194000 and 248,187040 and 2450,24530 and 0, 254231 and 4200,23140 and 300,38453 and 430,120232 and 1280, 212438 and 3600,143294 and 0 and 242940 and 1924 respectively. Conclusion: Conventional treatment combined with antimicrobial PDT found to be equall

Abstracts of National Conference on Biological, Biochemical, Biomedical, Bioenergy, and Environmental Biotechnology

This book contains the abstracts of the papers presented at the National Conference on Biological, Biochemical, Biomedical, Bioenergy, and Environmental Biotechnology (NCB4EBT-2021) Organized by the Department of Biotechnology, National Institute of Technology Warangal, India held on 29–30 January 2021. This conference is the first of its kind organized by NIT-W which covered an array of interesting topics in biotechnology. This makes it a bit special as it brings together researchers from different disciplines of biotechnology, which in turn will also open new research and cooperation fields for them. Conference Title: National Conference on Biological, Biochemical, Biomedical, Bioenergy, and Environmental BiotechnologyConference Acronym: NCB4EBT-2021Conference Date: 29–30 January 2021Conference Location: Online (Virtual Mode)Conference Organizer: Department of Biotechnology, National Institute of Technology Warangal, Indi