157 research outputs found
Free leucine dissociates homo- and heterodimers formed between proteins containing leucine heptad repeats
AbstractA highly specific method for the dissociation of protein dimers has been developed. The method involves exposure of the dimers to free leucine at a concentration ranging between 3 and 10 mM. Using this method it has been possible to dissociate goat uterine oestrogen receptor homodimers, heterodimers formed between the non-activated oestrogen receptor (naER) and the oestrogen receptor activation factor (E-RAF) of the goat uterus, c-jun homodimers derived from bovine bone marrow and also glucocorticoid receptor homodimers isolated from rat liver cytosol. The pattern of dimer dissociation by leucine clearly differentiates two classes of proteins. The first is represented by steroid hormone receptors where dimerization is apparently contributed by both coiled-coil dimerization interfaces and the conserved heptad repeats of leucine. The second is represented by oncoproteins like c-fos and c-jun which dimerize through the exclusive involvement of leucine zippers. The patterns of dissociation of these two groups of proteins from the concerned affinity columns are distinctly different. This indicates a possibility that the elution pattern may be used as a yardstick to determine whether two proteins dimerize through the exclusive involvement of leucine zippers or whether coiled-coil interfaces are also involved in the dimerization process
A Bulk-Parallel Priority Queue in External Memory with STXXL
We propose the design and an implementation of a bulk-parallel external
memory priority queue to take advantage of both shared-memory parallelism and
high external memory transfer speeds to parallel disks. To achieve higher
performance by decoupling item insertions and extractions, we offer two
parallelization interfaces: one using "bulk" sequences, the other by defining
"limit" items. In the design, we discuss how to parallelize insertions using
multiple heaps, and how to calculate a dynamic prediction sequence to prefetch
blocks and apply parallel multiway merge for extraction. Our experimental
results show that in the selected benchmarks the priority queue reaches 75% of
the full parallel I/O bandwidth of rotational disks and and 65% of SSDs, or the
speed of sorting in external memory when bounded by computation.Comment: extended version of SEA'15 conference pape
Registration of âALR 2â Peanut
'ALR 2' Spanish peanut (Arachis hypogaea L. subsp. fastigiata
Waldron var. vulgaris Hartz) (Reg. no. CV-61, P1 599975) is a
pure-line selection from an advanced breeding line, ICGV 86011,
developed at the International Crops Research Institute for the
Semi-Arid Tropics (ICRISAT), ICRISAT Asia Center (IAC),
Patancheru, AP, India. The original population of ICGV 86011 was
supplied to the Agricultural Research Station, Aliyarnagar, Tamil
Nadu, India, in 1984. It was tested during the rainy (Chitrai season:
April sowing, no irrigation), postrainy (Margazhi season: October
sowing, irrigated), and summer (Adi season: June sowing, irrigated)
seasons of 1986 to 1993 in various yield trials in Tamil Nadu.
After 8 yr of evaluation, ALR 2 was released in 1994 by the state
varietal release subcommittee of the Tamil Nadu Agricultural
University, Coimbatore, for cultivation in the rainy and irrigated
postrainy and summer seasons in the Pollachi tract of Tamil Nadu
(3)
Prevention of Hepatic Steatosis and Hepatic Insulin Resistance by Knockdown of cAMP Response Element-Binding Protein
SummaryIn patients with poorly controlled type 2 diabetes mellitus (T2DM), hepatic insulin resistance and increased gluconeogenesis contribute to fasting and postprandial hyperglycemia. Since cAMP response element-binding protein (CREB) is a key regulator of gluconeogenic gene expression, we hypothesized that decreasing hepatic CREB expression would reduce fasting hyperglycemia in rodent models of T2DM. In order to test this hypothesis, we used a CREB-specific antisense oligonucleotide (ASO) to knock down CREB expression in liver. CREB ASO treatment dramatically reduced fasting plasma glucose concentrations in ZDF rats, ob/ob mice, and an STZ-treated, high-fat-fed rat model of T2DM. Surprisingly, CREB ASO treatment also decreased plasma cholesterol and triglyceride concentrations, as well as hepatic triglyceride content, due to decreases in hepatic lipogenesis. These results suggest that CREB is an attractive therapeutic target for correcting both hepatic insulin resistance and dyslipidemia associated with nonalcoholic fatty liver disease (NAFLD) and T2DM
Applying a transformative consumer research lens to understanding and alleviating poverty
Increasing attention to global poverty and the development of market-based solutions for poverty alleviation continues to motivate a broad array of academicians and practitioners to better understand the lives of the poor. Yet, the robust perspectives residing within consumer research remain to a large degree under-utilized in these pursuits. This paper articulates how applying a transformative consumer research (TCR) lens to poverty and its alleviation can generate productive insights with potential to positively transform the well-being of poor consumers
Fractionation and characterization of oil palm (Elaeis guineensis) as treated by supercritical water
Moving Beyond Mimicry: Developing Hybrid Spaces in Indian Business Schools
This article analyses the identity work of Indian management educators and scholars as they seek to establish, maintain and revise a sense of self in the context of business school globalization. We show how globalization, combined with the historical legacy of colonialism, renders Indian scholars precarious in their interactions with Western business schools. Based on a qualitative interview study, we explore how Indian business school scholars perform their identities in the context of neo-colonial relations, which are characterised by the dominance of English language and a pressure to conform to research norms set by globally-ranked journals. Drawing on postcolonial theory, our argument focuses on mimicry as a distinctive form of identity work that involves maintaining difference between Western and non-Western identities by 'Othering' Indian scholars, while simultaneously seeking to transform them. We draw attention to ambivalence within participants' accounts, which we suggest arises because the authority of Western scholarship relies on maintaining non-Western scholars in a position of alterity or 'not quite-ness'. We suggest that hybridity offers an opportunity to disrupt and question current practices of business school globalization and facilitate scholarly engagement that reflects more diverse philosophical positions and worldviews
Voiceless but empowered farmers in corporate supply chains: contradictory imagery and instrumental approach to empowerment
There have been calls for a shift of focus towards the political and power-laden aspects of transitioning towards socially equitable global supply chains. This paper offers an empirically grounded response to these calls from a critical realist stance in the context of global food supply chains. We examine how an imaginary for sustainable farming structured around an instrumental construction of empowerment limits what is viewed as permissible, desirable and possible in global food supply chains. We adopt a multimodal Critical Discourse Analysis (CDA) to examine the sustainable farming imaginary for smallholder farmers constructed by one large organization, Unilever, in a series of videos produced and disseminated on YouTube. We expose the underlying mechanisms of power and marginalization at work within the sustainability imaginary and show how âempowermentâ has the potential to create of new dependencies for these farmers. We recontextualize the representations to show that while the imaginary may be commercially feasible, it is less achievable in terms of empowering smallholder farmers
System differentiation in England: the imposition of supply and demand
This chapter describes changing state and sector policy in relation to differentiation and how it has emerged in the English HE context: specifically, the attempts to concentrate the highest qualified applicants and the most prestigious institutions in a 'premium' market segment; the significance of the growing involvement of private providers; and the rise of the âstudent-as-consumerâ and 'value for money' in recent government policy discourse (e.g. the White Papers Students at the Heart of the System (DBIS 2011a) and Success as a Knowledge Economy (DBIS 2016). The chapter situates the development of a market hierarchy (in the form of a vertical differentiation of institutions, Archer 2007) following the demise of the university-polytechnic binary system in 1992 (Further and Higher Education Act, HMSO 1992). This co-existed for several years with the institutional diversity often celebrated by the Higher Education Funding Council for England (e.g. HEFCE 1994; 2000) that can be conceptualised as the horizontal differentiation of valued types of higher education provision and provider (e.g. part-time or vocationally orientated). The introduction of market mechanisms, in various stages beginning with the 2004 Higher Education Act (DfES 2004) and the introduction of variable tuition fees, coincided with the publication of institutional league tables from 2005. Taken together, these have reinforced a hierarchical system in which all institutions and courses are henceforth differentiated only by reference to a set of criteria dominated by the entry requirements demanded, and the amount of research carried out by the institution. Given the implications of the most recent legislation â the Higher Education and Research Act (HMSO 2017) this hierarchy is likely to be matched by one signalled by tuition fee levels, as new cheaper 'challenger' institutions come to the market
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