Utilization of Agro-Industrial Byproducts for Bacteriocin Production Using Newly Isolated Enterococcus faecium BS13

Microbial production of antimicrobials as biopreservatives is the major area of focus nowadays due to increased interest of consumers towards natural and safe preservation of ready to eat food products. The agro-industrial byproduct based medium and optimized process conditions can contribute in economical production of bacteriocins. Keeping this in view, the present investigation was carried out on agro-industrial byproducts utilization for the production of bacteriocin using Enterococcus faecium BS13 isolated from local fermented food. Different agro-industrial byproduct based carbon sources (whey, potato starch liquor, kinnow peel, deoiledrice bran and molasses), nitrogen sources (soya okra, pea pod and corn steep liquor), metal ions and surfactants were tested for optimal bacteriocin production. The effect of various process parameters such as pH, temperature, inoculum level, agitation and time were also tested on bacteriocin production. The optimized medium containing whey, supplemented with 4%corn steep liquor and polysorbate-80 displayed maximum bacteriocin activity with 2% inoculum, at pH 6.5, temperature 40oC under shaking conditions (100 rpm)

Distribution of the color fields around static quarks: Flux tube profiles

We report detailed calculations of the profiles of energy and action densities in the quark-antiquark string in SU(2) lattice gauge theory.Comment: 40 pages, LSUHE 94-15

Drag on a permeable sphere placed in a micropolar fluid with non-zero boundary condition for microrotations

This paper concerns an analytical study of an infinite expanse of uniform flow of steady axisymmetric creeping flow of an incompressible micropolar fluid around the permeable sphere assuming a nonhomogeneous boundary condition for microrotation vector. It is assumed that microrotation vector is proportional to the rotation rate of velocity vector. The stream function solutions for the flow fields are obtained in the terms of modified Bessel’s functions and Gegenbauer functions. Continuity of normal velocity, no-slip condition, non-zero microrotation vector on the sphere, uniform velocity at infinity are the different boundary conditions used to determine the flow fields explicitly. The microrotation component, pressure field, bounds of permeability parameter and drag force experienced by the permeable sphere are calculated. Dependence of the drag force on different fluid parameters is presented graphically and discussed. It is found that drag force decreases with increasing spin parameter. Several cases of interest are deduced from the present analysis

A Recurrent De Novo Variant in NACC1 Causes a Syndrome Characterized by Infantile Epilepsy, Cataracts, and Profound Developmental Delay.

Whole-exome sequencing (WES) has increasingly enabled new pathogenic gene variant identification for undiagnosed neurodevelopmental disorders and provided insights into both gene function and disease biology. Here, we describe seven children with a neurodevelopmental disorder characterized by microcephaly, profound developmental delays and/or intellectual disability, cataracts, severe epilepsy including infantile spasms, irritability, failure to thrive, and stereotypic hand movements. Brain imaging in these individuals reveals delay in myelination and cerebral atrophy. We observe an identical recurrent de novo heterozygous c.892C>T (p.Arg298Trp) variant in the nucleus accumbens associated 1 (NACC1) gene in seven affected individuals. One of the seven individuals is mosaic for this variant. NACC1 encodes a transcriptional repressor implicated in gene expression and has not previously been associated with germline disorders. The probability of finding the same missense NACC1 variant by chance in 7 out of 17,228 individuals who underwent WES for diagnoses of neurodevelopmental phenotypes is extremely small and achieves genome-wide significance (p = 1.25 × 10-14). Selective constraint against missense variants in NACC1 makes this excess of an identical missense variant in all seven individuals more remarkable. Our findings are consistent with a germline recurrent mutational hotspot associated with an allele-specific neurodevelopmental phenotype in NACC1