Simulations of Adaptive Optics with a Laser Guide Star for SINFONI

The SINFONI instrument for ESO's VLT combines integral field spectroscopy and adaptive optics (AO). We discuss detailed simulations of the adaptive optics module. These simulations are aimed at assessing the AO module performance, specifically for operations with extended sources and a laser guide star. Simulated point spread function (PSF) images will be used to support scientific preparations and the development of an exposure time calculator, while simulated wavefront sensor measurements will be used to study PSF reconstruction methods. We explain how the adaptive optics simulations work, focusing on the realistic modelling of the laser guide star for a curvature wavefront sensor. The predicted performance of the AO module is discussed, resulting in recommendations for the operation of the SINFONI AO module at the telescope.Comment: 12 pages, 6 figures, to appear in SPIE conference proceedings vol 5490, "Advancements in Adaptive Optics", eds. D. Bonaccini, B.L. Ellerbroek, R. Ragazonni, Glasgow UK, 21-25 June 200

First CO J=6-5, 4-3 detections in local ULIRGs: the dense gas in Mrk231, and its colling budget

We report on detections of the high-excitation CO J=6-5, J=4-3 lines in Mrk231, a prototypical Ultra Luminous Infrared Galaxy (ULIRG) and Seyfert 1 QSO. These observations are combined with CO J=3-2, HCN J=4-3 (this work), and CO J=2-1, J=1-0, 13CO J=2-1, HCN J=1-0 measurements taken from the literature to provide better constraints on the properties of the molecular gas in an extreme starburst/QSO in the local Universe. We find that the CO J=4-3 and J=6-5 transitions trace a different gas phase from that dominating the lower three CO transitions, with n(H_2) ~ (1-3)x10^4 cm-3 and Tk ~ (40-70) K. This phase is responsible for the luminous HCN emission, and contains most of the H2 gas mass of this galaxy. The total CO line cooling emanating from this dense phase is found similar to that of the [CII] line at 158 micron, suggesting a very different thermal balance to that seen in lower IR-luminosity galaxies, and one likely dominated by dense photon-dominated regions. Our dense "sampling" of the CO rotational ladder and the HCN lines enables us to produce well-constrained Spectral Line Energy Distributions (SLEDs) for the dense molecular gas in Mrk231 and compare them to those of high redshift starbursts, many of which have SLEDs that may be affected by strong lensing. Finally, we use our local molecular line excitation template to assess the capabilities of future cm and mm/sub-mm arrays in detecting CO and HCN transitions in similar systems throughout the local and distant universe.Comment: accepted for publication in The Astrophysical Journal; 37 pages, preprint format; 5 figures (2 in color

Te hooi en te gras. Verkenning naar ontwikkeling in de grondgebonden veehouderij en gevolgen hiervan voor natuur en landschap

Onderzoek naar de relatie tuusen landbouwkundig grondgebruik en natuur; analyse ten behoeve van het Natuurplanburea

Ruimte voor akkers en tuinen, bomen en bollen : verkenning naar ontwikkelingen in de akkerbouw en opengrondstuinbouw en effecten hiervan op natuur en landschap

Invloed van landbouwkundig grondgebruik op natuur en alndschap: een inventaristatie door Natuurplanburea

Fire in the Heart: A Characterization of the High Kinetic Temperatures and Heating Sources in the Nucleus of NGC253

The nuclear starburst within the central $\sim 15^{\prime\prime}$ ($\sim 250$ pc; $1^{\prime\prime} \simeq 17$ pc) of NGC253 has been extensively studied as a prototype for the starburst phase in galactic evolution. Atacama Large Millimeter/submillimeter Array (ALMA) imaging within receiver Bands 6 and 7 have been used to investigate the dense gas structure, kinetic temperature, and heating processes which drive the NGC253 starburst. Twenty-nine transitions from fifteen molecular species/isotopologues have been identified and imaged at $1.^{\prime\prime}5$ to $0.^{\prime\prime}4$ resolution, allowing for the identification of five of the previously-studied giant molecular clouds (GMCs) within the central molecular zone (CMZ) of NGC253. Ten transitions from the formaldehyde (H$_2$CO) molecule have been used to derive the kinetic temperature within the $\sim 0.^{\prime\prime}5$ to $5^{\prime\prime}$ dense-gas structures imaged. On $\sim 5^{\prime\prime}$ scales we measure $T_K \gtrsim 50$ K, while on size scales $\lesssim 1^{\prime\prime}$ we measure $T_K \gtrsim 300$ K. These kinetic temperature measurements further delineate the association between potential sources of dense gas heating. We have investigated potential heating sources by comparing our measurements to models which predict the physical conditions associated with dense molecular clouds that possess a variety of heating mechanisms. This comparison has been supplemented with tracers of recently-formed massive stars (Br$\gamma$) and shocks ([FeII]). Derived molecular column densities point to a radially-decreasing abundance of molecules with sensitivity to cosmic ray and mechanical heating within the NGC253 CMZ. These measurements are consistent with radio spectral index calculations which suggest a higher concentration of cosmic ray producing supernova remnants within the central 10 pc of NGC253.Comment: 60 pages, 25 figures (whew!), Accepted for publication in ApJ, Latest version includes minor corrections following proof submissio

Fire-Related Carbon Emissions from Land Use Transitions in Southern Amazonia

Various land-use transitions in the tropics contribute to atmospheric carbon emissions, including forest conversion for small-scale farming, cattle ranching, and production of commodities such as soya and palm oil. These transitions involve fire as an effective and inexpensive means for clearing. We applied the DECAF (DEforestation CArbon Fluxes) model to Mato Grosso, Brazil to estimate fire emissions from various land-use transitions during 2001-2005. Fires associated with deforestation contributed 67 Tg C/yr (17 and 50 Tg C/yr from conversion to cropland and pasture, respectively), while conversion of savannas and existing cattle pasture to cropland contributed 17 Tg C/yr and pasture maintenance fires 6 Tg C/yr. Large clearings (>100 ha/yr) contributed 67% of emissions but comprised only 10% of deforestation events. From a policy perspective, results imply that intensification of agricultural production on already-cleared land and policies to discourage large clearings would reduce the major sources of emissions from fires in this region. Copyright 2008 by the American Geophysical Union

Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies

Replication-defective (RD) recombinant simian virus 40 (SV40)-based gene delivery vectors hold a great potential for clinical applications because of their presumed non-immunogenicity and capacity to induce immune tolerance to the transgene products in humans. However, the clinical use of SV40 vectors has been hampered by the lack of a packaging cell line that produces replication-competent (RC) free SV40 particles in the vector production process. To solve this problem, we have adapted the current SV40 vector genome used for the production of vector particles and generated a novel Vero-based packaging cell line named SuperVero that exclusively expresses the SV40 large T antigen. SuperVero cells produce similar numbers of SV40 vector particles compared to the currently used packaging cell lines, albeit in the absence of contaminating RC SV40 particles. Our unique SV40 vector platform named SVac paves the way to clinically test a whole new generation of SV40-based therapeutics for a broad range of important diseases