32 research outputs found
Π ΡΠ²Π½ΡΠ½Π½Ρ Π΅Π»Π΅ΠΊΡΡΠΎΠΌΠ°Π³Π½ΡΡΠ½ΠΎΡ ΠΌΠ΅Ρ Π°Π½ΡΠΊΠΈ ΠΏΠΎΡΠΈΡΡΠΎΠ³ΠΎ Π½Π°ΡΠΈΡΠ΅Π½ΠΎΠ³ΠΎ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΠ°
ΠΠ° Π΄Π²ΠΎΠΊΠΎΠ½ΡΠΈΠ½ΡΡΠΌΠ½ΠΎΠ³ΠΎ Π½Π°Π±Π»ΠΈΠΆΠ΅Π½Π½Ρ ΠΎΡΡΠΈΠΌΠ°Π½ΠΎ ΠΏΠΎΠ²Π½Ρ ΡΠΈΡΡΠ΅ΠΌΡ ΡΠΏΡΠ²Π²ΡΠ΄Π½ΠΎΡΠ΅Π½Ρ ΠΌΠΎΠ΄Π΅Π»Ρ Π΅Π»Π΅ΠΊΡΡΠΎΠΌΠ°Π³Π½ΡΡΠΎΠΌΠ΅Ρ
Π°Π½ΡΠΊΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ½ΠΎ ΠΎΠ΄Π½ΠΎΡΡΠ΄Π½ΠΎΠ³ΠΎ ΡΠ° ΡΠ·ΠΎΡΡΠΎΠΏΠ½ΠΎΠ³ΠΎ ΠΏΠΎΡΠΈΡΡΠΎΠ³ΠΎ Π½Π°ΡΠΈΡΠ΅Π½ΠΎΠ³ΠΎ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΠ°. ΠΡΠ°Ρ
ΠΎΠ²Π°Π½ΠΎ Π½Π°ΡΠ²Π½ΡΡΡΡ ΠΏΠΎΠ΄Π²ΡΠΉΠ½ΠΎΠ³ΠΎ Π΅Π»Π΅ΠΊΡΡΠΈΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΡΡ Π² ΠΎΠΊΠΎΠ»Ρ ΠΌΠ΅ΠΆΡ ΠΊΠΎΠ½ΡΠ°ΠΊΡΡ ΡΠ²Π΅ΡΠ΄ΠΎΡ Ρ ΡΡΠ΄ΠΊΠΎΡ ΡΠ°Π·.Complete set of the model equations for electromagnetic mechanics of the porous saturated medium being statistically homogeneous and isotropic is obtained in two-continuum approximation. The presence of a double electrical layer in an environ of contact boundary of solid and liquid phases is taken into account.Π Π΄Π²ΡΡ
ΠΊΠΎΠ½ΡΠΈΠ½ΡΡΠΌΠ½ΠΎΠΌ ΠΏΡΠΈΠ±Π»ΠΈΠΆΠ΅Π½ΠΈΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΎ ΠΏΠΎΠ»Π½ΡΡ ΡΠΈΡΡΠ΅ΠΌΡ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΠΌΠ°Π³Π½ΠΈΡΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΠΊΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ ΠΎΠ΄Π½ΠΎΡΠΎΠ΄Π½ΠΎΠΉ ΠΈ ΠΈΠ·ΠΎΡΡΠΎΠΏΠ½ΠΎΠΉ ΠΏΠΎΡΠΈΡΡΠΎΠΉ Π½Π°ΡΡΡΠ΅Π½Π½ΠΎΠΉ ΡΡΠ΅Π΄Ρ. Π£ΡΡΠ΅Π½ΠΎ Π½Π°Π»ΠΈΡΠΈΠ΅ Π΄Π²ΠΎΠΉΠ½ΠΎΠ³ΠΎ ΡΠ»Π΅ΠΊΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ»ΠΎΡ Π² ΠΎΠΊΡΠ΅ΡΡΠ½ΠΎΡΡΠΈ Π³ΡΠ°Π½ΠΈΡΡ ΠΊΠΎΠ½ΡΠ°ΠΊΡΠ° ΡΠ²Π΅ΡΠ΄ΠΎΠΉ ΠΈ ΠΆΠΈΠ΄ΠΊΠΎΠΉ ΡΠ°Π·
Integrative Transkingdom Analysis of the Gut Microbiome in Antibiotic Perturbation and Critical Illness
cited By 2Bacterial microbiota play a critical role in mediating local and systemic immunity, and shifts in these microbial communities have been linked to impaired outcomes in critical illness. Emerging data indicate that other intestinal organisms, including bacteriophages, viruses of eukaryotes, fungi, and protozoa, are closely interlinked with the bacterial microbiota and their host, yet their collective role during antibiotic perturbation and critical illness remains to be elucidated. We employed multi-omics factor analysis (MOFA) to systematically integrate the bacterial (16S rRNA), fungal (intergenic transcribed spacer 1 rRNA), and viral (virus discovery next generation sequencing) components of the intestinal microbiota of 33 critically ill patients with and without sepsis and 13 healthy volunteers. In addition, we quantified the absolute abundances of bacteria and fungi using 16S and 18S rRNA PCRs and characterized the short-chain fatty acids (SCFAs) butyrate, acetate, and propionate using nuclear magnetic resonance spectroscopy. We observe that a loss of the anaerobic intestinal environment is directly correlated with an overgrowth of aerobic pathobionts and their corresponding bacteriophages as well as an absolute enrichment of opportunistic yeasts capable of causing invasive disease. We also observed a strong depletion of SCFAs in both disease states, which was associated with an increased absolute abundance of fungi with respect to bacteria. Therefore, these findings illustrate the complexity of transkingdom changes following disruption of the intestinal bacterial microbiome. IMPORTANCE While numerous studies have characterized antibiotic-induced disruptions of the bacterial microbiome, few studies describe how these disruptions impact the composition of other kingdoms such as viruses, fungi, and protozoa. To address this knowledge gap, we employed MOFA to systematically integrate viral, fungal, and bacterial sequence data from critically ill patients (with and without sepsis) and healthy volunteers, both prior to and following exposure to broad-spectrum antibiotics. In doing so, we show that modulation of the bacterial component of the microbiome has implications extending beyond this kingdom alone, enabling the overgrowth of potentially invasive fungi and viruses. While numerous preclinical studies have described similar findings in vitro, we confirm these observations in humans using an integrative analytic approach. These findings underscore the potential value of multi-omics data integration tools in interrogating how different components of the microbiota contribute to disease states. In addition, our findings suggest that there is value in further studying potential adjunctive therapies using anaerobic bacteria or SCFAs to reduce fungal expansion after antibiotic exposure, which could ultimately lead to improved outcomes in the intensive care unit (ICU).Peer reviewe
Plasma protein N-glycosylation is associated with cardiovascular disease, nephropathy, and retinopathy in type 2 diabetes
Introduction Although associations of total plasma N-glycome (TPNG) with type 2 diabetes have been reported, little is known on the role of TPNG in type 2 diabetes complications, a major cause of type 2 diabetes-related morbidity and mortality. Here, we assessed TPNG in relation to type 2 diabetes complications in subsamples of two Dutch cohorts using mass spectrometry (n=1815 in DiaGene and n=1518 in Hoorn Diabetes Care System).Research design and methods Blood plasma samples and technical replicates were pipetted into 96-well plates in a randomized manner. Peptide:N-glycosidase F (PNGase F) was used to release N-glycans, whereafter sialic acids were derivatized for stabilization and linkage differentiation. After total area normalization, 68 individual glycan compositions were quantified in total and were used to calculate 45 derived traits which reflect structural features of glycosylation. Associations of glycan features with prevalent and incident microvascular or macrovascular complications were tested in logistic and Cox regression in both independent cohorts and the results were meta-analyzed.Results Our results demonstrated similarities between incident and prevalent complications. The strongest association for prevalent cardiovascular disease was a high level of bisection on a group of diantennary glycans (A2FS0B; OR=1.38, p=1.34x10(-11)), while for prevalent nephropathy the increase in 2,6-sialylation on triantennary glycans was most pronounced (A3E; OR=1.28, p=9.70x10(-6)). Several other TPNG features, including fucosylation, galactosylation, and sialylation, firmly demonstrated associations with prevalent and incident complications of type 2 diabetes.Conclusions These findings may provide a glance on how TPNG patterns change before complications emerge, paving the way for future studies on prediction biomarkers and potentially disease mechanisms.Molecular Epidemiolog
Cholesterol-lowering effects of metformin in APOE*3-Leiden.CETP mice, a transgenic model with a human-like lipoprotein profile
Diabetes mellitus: pathophysiological changes and therap
Cholesterol-lowering effects of metformin in APOE*3-Leiden.CETP mice, a transgenic model with a human-like lipoprotein profile
Aliskiren inhibits atherosclerosis development and improves plaque stability in APOEM*3Leiden.CETP transgenic mice with or without treatment with atorvastatin
Diabetes mellitus: pathophysiological changes and therap
Shifting priorities in the aftermath of a Q fever epidemic in 2007 to 2009 in The Netherlands: from acute to chronic infection.
Contains fulltext :
109522.pdf (publisher's version ) (Open Access)From 2007 to 2009, the Netherlands faced large seasonal outbreaks of Q fever, in which infected dairy goat farms were identified as the primary sources. Veterinary measures including vaccination of goats and sheep and culling of pregnant animals on infected farms seem to have brought the Q fever problem under control. However, the epidemic is expected to result in more cases of chronic Q fever among risk groups in the coming years. In the most affected area, in the south of the country, more than 12% of the population now have antibodies against Coxiella burnetii. Questions remain about the follow-up of acute Q fever patients, screening of groups at risk for chronic Q fever, screening of donors of blood and tissue, and human vaccination. There is a considerable ongoing research effort as well as enhanced veterinary and human surveillance
APOE*3LEIDEN.CETP TRANSGENIC MICE AS MODEL FOR THE METABOLIC SYNDROME
Diabetes mellitus: pathophysiological changes and therap
Chronic Q fever-related complications and mortality: data from a nationwide cohort
Item does not contain fulltex