Genetic study in patients operated dentally and anesthetized with articaine-epinephrine

Aims: In this study we wanted to figure out if there was a correlation between OPRM1 N40D, TRPV1 I316M, TRPV1 I585V, NOS3 −786T>C and IL6 −174C>G polymorphisms and the response to locally applied articaine-epinephrine anesthetic. Methods: In this observational study, 114 oral cell samples of patients anesthetized with articaine-epinephrine (54 from men 60 from women), were collected from dental centers in Madrid (Spain). High molecular weight DNA was obtained from oral mucosa cells. The analysis of OPRM1 N40D (rs1799971), TRPV1 I316M (rs222747), TRPV1 I585V (rs8065080) and IL6 −174C>G polymorphism was performed through real-time PCR allelic discrimination using TaqMan probes. Polymorphism NOS3 −786T> C (rs2070744) was analyzed using RFLP-PCR. Results: The studied polymorphisms are involved neither in the response to the anesthetic, nor in the intensity of perceived dental pain. However, in a subset of female patients we found that TRPV1 I316M was associated with a delayed onset of anesthesia. Conclusions: There is no association among these polymorphisms and the time elapsed between the application of the anesthetic and the onset of its effect

Understanding Musicking on Social Media: Music Sharing, Sociality and Citizenship

Based on online and offline ethnographic fieldwork among the Spanish community in London, this thesis investigates why people circulate and share music on social media platforms, particularly within a context of migration. Written from the perspective of an insider-outsider, it combines research data from ethnographic participant observation and interviews to understand how music media circulates online, studying the different roles it fulfils within these contexts as different forms of musicking. The thesis responds to these questions by addressing six areas. Chapter 1 explores the uses of music to perform and articulate cultural and gender identity on social media profiles. Chapter 2 investigates how music sharing and compiling can be used to maintain relationships and social capital and to participate in transnational sociality. Chapter 3 addresses how the circulation of music parody as citizen engagement in contexts of political conflict such as the Brexit and Catalonian referendums gives rise to temporary political alliances in the form of citizen assemblages. Chapter 4 considers the circulation of music on social media from the perspective of fandom and how it is influenced by post-object ephemeral and reflexive practices. Chapter 5 analyses how the ubiquitous and imagined character of online music media fosters its circulation as a silent and visual element of online sociality, effectively generating practices of imagined listening. Chapter 6 examines ritualistic practices of music exchange on social media and their relationship with emerging moral economies of music circulation as foundational elements of online sociality and citizenship. I conclude by arguing that, in circulating music online through their social media and streaming profiles, users develop new forms of (im)material culture-making. Online musicking enables new ways of being in the world, turning the intangible, time-bound aural and visual experience of online music into something that has a materialised impact in the social lives of users. Through the circulation of music online users form, dissolve and inhabit temporary music-based alliances and expand their social worlds

A Rare Presentation of Invasive Tuberculosis of the Central Nervous System in an Immunocompetent Patient in a Nonendemic Country.

We herein report a rare case of a 25-year-old immunocompetent male patient with disseminated tuberculosis of central nervous system (CNS), first presenting as multiple cerebral lesions with no meningeal involvement. Subsequent diagnostic workup disclosed extensive peritoneal involvement. A broad differential diagnosis was considered, including neoplastic and infectious diseases. The diagnosis was confirmed with positive PCR result for Mycobacterium tuberculosis in the biopsied mesenteric tissue. The patient was started on tuberculostatic regimen with favorable outcome. No acquired or hereditary immunodeficiency was documented. Disseminated tuberculosis in immunocompetent individuals is extremely rare. Genetic susceptibility factors have been reported in individuals with extensive forms of the disease and a high index of suspicion is required, as observed in our case.info:eu-repo/semantics/publishedVersio

Surveillance studies of Lymphocystis disease virus in farmed gilthead seabream (Sparus aurata) by real-time PCR

Lymphocystis disease (LCD) is the main viral infection reported to affect cultured gilthead seabream (Sparus aurata) in Southern Atlantic and Mediterranean aquaculture. Its etiological agent is the Lymphocystis disease virus (LCDV), a member of the family Iridoviridae (genus Lymphocystivirus). The only adequate measures for LCD prevention in the aquaculture systems are general prophylactic practices, such as the control of fish to be introduced in the farm facilities in order to detect carrier fish. These animals may pose a risk for the introduction of LCDV in fish farms, as direct contact between fish specimens is considered the main route of LCDV spreading. More recently, asymptomatic carrier breeders, as well as virus contaminated-live food, have been involved in LCDV transmission to fish larvae. The detection of subclinical viral infections in carrier fish requires the use of sensitive diagnostic methods. In this context, the objective of this study was to establish the applicability of a real-time PCR assay for LCDV diagnosis in surveillance studies. In addition, the assay has been evaluated with samples from a gilthead seabream hatchery, in order to prove its utility to trace the origin of LCDV in fish farms. Juvenile fish were collected at four farms with different background regarding to LCD. LCDV was detected in all farms, and 30 to 100% of fish were identified as LCDV-infected. Estimated viral load in caudal fin of asymptomatic fish was two to five orders of magnitude lower than in diseased fish. Carrier fish were also identified in the broodstock from a farm with LCD records by analysing caudal fin samples by qPCR. In this farm, the q-PCR assay developed in this study allowed the quantitative detection of LCDV in all samples collected in the hatchery, including fertilized eggs, larvae and fingerlings, and also rotifer cultures and artemia metanauplii and cysts used for larval rearing.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Logarithmic growth dynamics in software networks

In a recent paper, Krapivsky and Redner (Phys. Rev. E, 71 (2005) 036118) proposed a new growing network model with new nodes being attached to a randomly selected node, as well to all ancestors of the target node. The model leads to a sparse graph with an average degree growing logarithmically with the system size. Here we present compeling evidence for software networks being the result of a similar class of growing dynamics. The predicted pattern of network growth, as well as the stationary in- and out-degree distributions are consistent with the model. Our results confirm the view of large-scale software topology being generated through duplication-rewiring mechanisms. Implications of these findings are outlined.Comment: 7 pages, 3 figures, published in Europhysics Letters (2005

The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021

In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the smc03787 open reading frame (ORF). No well-predicted RirA or Irr boxes were found in the region immediately upstream of the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787. We demonstrate that bfr gene expression is enhanced under iron-sufficient conditions and that Irr and RirA modulate this expression. The pattern of bfr gene expression as well as the response to Irr and RirA is inversely correlated to that of smc03787. Moreover, our results suggest that the small RNA SmelC759 participates in RirA- and Irr-mediated regulation of bfr expression and that additional unknown factors are involved in iron-dependent regulation.Fil: Costa, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Amarelle, Vanesa. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: O`Brian, Mark R.. State University of New York; Estados UnidosFil: Fabiano, Elena. Instituto de Investigaciones Biológicas "Clemente Estable"; Urugua

Two dimensional XXZ-Ising model on square-hexagon lattice

We study a two dimensional XXZ-Ising on square-hexagon (4-6) lattice with spin-1/2. The phase diagram of the ground state energy is discussed, shown two different ferrimagnetic states and two type of antiferromagnetic states, beside of a ferromagnetic state. To solve this model, it could be mapped into the eight-vertex model with union jack interaction term. Imposing exact solution condition we find the region where the XXZ-Ising model on 4-6 lattice have exact solutions with one free parameter, for symmetric eight-vertex model condition. In this sense we explore the properties of the system and analyze the competition of the interaction parameters providing the region where it has an exact solution. However the present model does not satisfy the \textit{free fermion} condition, unless for a trivial situation. Even so we are able to discuss their critical points region, when the exactly solvable condition is ignored.Comment: 5 pages, 5 figure

Un procedimiento nuevo para adaptar cualquier tipo de suelo para la construcción y consolidación de estructuras de tierra: Sistema de Tierra Proyectada

The “projected earth system” is put forward as an alternative construction method. The soil from each site is adapted following a specific protocol to make it suitable for spraying. The type of construction and the receiving surface determine the machinery and spraying system used (dry or wet mix). The result will be similar in texture and colour to the original material (in the case of earth walls) or to the surrounding material.Un procedimiento nuevo para adaptar cualquier tipo de suelo para la construcción y consolidación de estructuras de tierra: Sistema de Tierra Proyectada. Se plantea la “tierra proyectada” como nuevo sistema constructivo. Se adecuará la tierra de cada intervención con un protocolo para poder ser proyectada. El tipo de intervención y el soporte sobre el que se proyectará determinarán la maquinaria y el sistema de proyección (vía seca o húmeda). El resultado tendrá similar textura y color que el soporte restaurado (en muros de tierra) o que el material del entorno (en taludes carreteros u obras de tierra de nueva construcción)