Biological activity of phenolic compounds from argentinean herbs infusions

Argentinean herbal infusions were studied for their beneficial biological properties and secondary metabolites content, such as phenolic compounds in order to find new natural products beneficial. The modifications of antihypertensive and antioxidant activities by the addition of commonly used additives (lemon, sugar and sweetener) were studied also. Ilex paraguaiensis infusion showed the highest concentration of phenolic compounds and flavonoid fraction, the supplementation with lemon juice increased the phenolic compound content. All infusion showed high DPPH radical scavenging assays and additives did not modify significantly this activity. The infusion of I. paraguaiensis presents the maximum viability reduction of Escherichia coli and Staphylococcus aureus and the herbs combination Lippia integrifolia-Ilex paraguaiensis was the most effective to reduce S. aureus and E.coli viability. The phenolic compounds concentration as well as the qualitative composition of each infusion plays an important role in the antimicrobial activity. Because the high correlation between the concentration of total phenolic compounds and antioxidant and antihypertensive activities, we can infer that the phenolic compounds are responsible for such activities. The results obtained allow us to propose the herbs studied as potential natural antioxidants and antihypertensive compounds that could be used in pharmaceutical and food industries.Fil: Rodriguez Vaquero, Maria Jose. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vallejo, Claudia Veronica. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aredes Fernández, Pedro Adrián. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Inactivation of Listeria monocytogenes and Salmonella Typhimurium in strawberry juice enriched with strawberry polyphenols

BACKGROUND: Low molecular-weight phenolic fractions (LMPFs) were extracted from Albion (LMPF-A) and Camarosa (LMPF-C) strawberry cultivars. Their antibacterial activity against Listeria monocytogenes and Salmonella Typhimurium cocktails in vitro and in vivo was investigated using strawberry juice as a food model. This study also sought to determine their antibacterial mechanism. RESULTS: Quercetin was identified as a principal compound in both phenolic fractions. The minimum bactericide concentration (MBC) values were 750 and 850 μg mL−1 (LMPF-C) and 800 and 950 μg mL−1 (LMPF-A) against S.Typhimurium and L. monocytogenes, respectively. The possible antibacterial activity of the phenolic extracts could be related to the release of phosphate and potassium ions, the effect of the disruption of membrane integrity on L. monocytogenes, and the effect of the inhibition of dihydronicotinamide adenine dinucleotide (NADH) oxidase activity on S. Typhimurium. Quercetin and kaempferol were the most active compounds in producing bacterial damage. Strawberry juice supplemented with the phenolic fractions and incubated at 37, 20, and 4 °C reduced bacterial viability; moreover, after treatment with the phenolic fraction at the lowest temperature, no viable cells were detected after 7 days’ incubation. Salmonella was more sensitive to the supplements than Listeria in strawberry juice. CONCLUSIONS: This study could form the basis for the development of natural antibacterial agents that could be included in natural juice or used by the pharmaceutical industry.Fil: Vallejo, Claudia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; ArgentinaFil: Minahk, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Rollan, Graciela Celestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Rodriguez Vaquero, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; Argentin

High cell-free DNA is associated with disease progression, inflammasome activation and elevated levels of inflammasome-related cytokine IL-18 in patients with myelofibrosis

Myelofibrosis (MF) is a clonal hematopoietic stem cell disorder classified among chronic myeloproliferative neoplasms, characterized by exacerbated myeloid and megakaryocytic proliferation and bone marrow fibrosis. It is induced by driver (JAK2/CALR/MPL) and high molecular risk mutations coupled to a sustained inflammatory state that contributes to disease pathogenesis. Patient outcome is determined by stratification into risk groups and refinement of current prognostic systems may help individualize treatment decisions. Circulating cell-free (cf)DNA comprises short fragments of double-stranded DNA, which promotes inflammation by stimulating several pathways, including inflammasome activation, which is responsible for IL-1β and IL-18 maturation and release. In this work, we assessed the contribution of cfDNA as a marker of disease progression and mediator of inflammation in MF. cfDNA was increased in MF patients and higher levels were associated with adverse clinical outcome, a high-risk molecular profile, advanced disease stages and inferior overall survival, indicating its potential value as a prognostic marker. Cell-free DNA levels correlated with tumor burden parameters and markers of systemic inflammation. To mimic the effects of cfDNA, monocytes were stimulated with poly(dA:dT), a synthetic double-stranded DNA. Following stimulation, patient monocytes released higher amounts of inflammasome-processed cytokine, IL-18 to the culture supernatant, reflecting enhanced inflammasome function. Despite overexpression of cytosolic DNA inflammasome sensor AIM2, IL-18 release from MF monocytes was shown to rely mainly on the NLRP3 inflammasome, as it was prevented by NLRP3-specific inhibitor MCC950. Circulating IL-18 levels were increased in MF plasma, reflecting in vivo inflammasome activation, and highlighting the previously unrecognized involvement of this cytokine in MF cytokine network. Monocyte counts were higher in patients and showed a trend towards correlation with IL-18 levels, suggesting monocytes represent a source of circulating IL-18. The close correlation shown between IL-18 and cfDNA levels, together with the finding of enhanced DNA-triggered IL-18 release from monocytes, suggest that cfDNA promotes inflammation, at least in part, through inflammasome activation. This work highlights cfDNA, the inflammasome and IL-18 as additional players in the complex inflammatory circuit that fosters MF progression, potentially providing new therapeutic targets.Fil: de Luca, Geraldine. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Lev, Paola Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Camacho, Maria F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Goette, Nora Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Sackmann, Federico. Fundación Para Combatir la Leucemia; ArgentinaFil: Castro Ríos, Miguel A.. No especifíca;Fil: Moiraghi, Beatriz. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Cortes Guerrieri, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Bendek, Georgina. Hospital Italiano; ArgentinaFil: Carricondo, Emiliano. Universidad Austral. Hospital Universitario Austral; ArgentinaFil: Enrico, Alicia. Hospital Italiano; ArgentinaFil: Vallejo, Veronica. Instituto Cardiovascular de Buenos Aires; ArgentinaFil: Varela, Ana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Khoury, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Gutierrez, Marina. Laboratorio Stamboulian; ArgentinaFil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Marta, Rosana Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Glembotsky, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Heller, Paula Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentin

Capacidad inhibitoria del escobajo, desecho de la industria vitivinícola, sobre cepas de (candida albicans) aisladas de pacientes con candidiasis vulvovaginal

Introducción. La Candidiasis Vulvovaginal (CVV) es una infección micótica de alta incidencia y recurrencia que causa dolor, irritación y prurito en la zona afectada. El 75% de las mujeres sufren por lo menos un episodio a lo largo de su vida. El agente etiológico más frecuente es Candida albicans. El tratamiento consiste principalmente en el uso de azoles antifúngicos, que provocan numerosos efectos secundarios y aparición de cepas resistentes. Por lo tanto, la búsqueda de nuevos agentes antimicóticos es necesaria y una posible fuente la representan los metabolitos de los residuos de la industria vitivinícola. El escobajo, material vegetal que soporta el racimo de la uva, conserva junto con el orujo un alto contenido de polifenoles luego del proceso de elaboración del vino.Objetivo. Evaluar la capacidad inhibidora de los extractos ricos en compuestos fenólicos del escobajo, residuo de la industria vitivinícola, sobre cepas de Candida albicans aisladas de muestras clínicas. Metodología. El extracto de escobajo (EE) se obtuvo por extracción continua en Soxhlet usando etanol como solvente. Se cuantificaron los compuestos fenólicos totales del EE y se evaluó su toxicidad sobre la viabilidad, el desarrollo larval y la reproducción de Caenorhaditis elegans. Se determinó la actividad antifúngica in vitro del EE (400, 600 y 800 µg/mL) sobre 2 cepas de Candida albicans (IBL001 e INM982891) mediante métodos de difusión en agar y de entrecruzamiento. Se determinó la capacidad del EE para inhibir el crecimiento (CIM) y la formación de biofilm de ambas cepas de C. albicans. La capacidad protectora de la muerte causada por cándida se estudió in vivo sobre el nematodo C. elegans infectado con IBL001 y INM982891.Resultados. El rendimiento del EE fue de 15%, lo que indica que la técnica de extracción utilizada fue apropiada. El EE inhibió el crecimiento de ambas cepas de C. albicans ensayadas (CIM 650 µg/mL) y la formación del biofilm por las mismas en ∼90%. Por otro lado, no alteró la viabilidad, el desarrollo normal ni la reproducción de C. elegans a las concentraciones ensayadas. El EE redujo en un 18% la muerte causada por la infección con las cepas IBL001 e INM982891 en el nematodo, utilizando 400 y 800 µg/mL, respectivamente para cada una. Este efecto fue similar al efecto protector de 1 mg/mL de Fluconazol, usado como control. Conclusiones. El EE fue efectivo para inhibir in vitro el crecimiento y el biofilm de dos cepas de C. albicans aisladas de pacientes con vulvovaginitis. Este efecto se vio reflejado en el ensayo in vivo, protegiendo al nematodo de la muerte causada por el patógeno. Dada la necesidad de encontrar nuevos agentes antimicóticos para el tratamiento de CVV causada por C. albicans, y de hallar formas alternativas de revalorizar y utilizar los desechos de las industrias ricos en metabolitos activos, el extracto de escobajo demostró tener actividad antifúngica para ser explotada en el desarrollo de nuevos antimicóticos.Fil: D'almeida, Romina Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vallejo, Claudia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; ArgentinaFil: Perez Merello, Mercedes. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; ArgentinaFil: Vera, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Rodriguez Vaquero, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; ArgentinaXV Congreso Argentino de Microbiología; V Congreso Argentino de Microbiología de Alimentos V Congreso Latinoamericano de Microbiología de Medicamentos y Cosméticos; XIV Congreso Argentino de Microbiología GeneralCiudad Autónoma de Buenos AriesArgentinaAsociación Argentina de Microbiologí

The impact of glucocorticoids and anti-cd20 therapy on cervical human papillomavirus infection risk in women with systemic lupus erythematosus

OBJECTIVE: To identify the prevalence and factors associated with cervical human papillomavirus infection in women with systemic lupus erythematosus METHODS: This cross-sectional study collected traditional and systemic lupus erythematosus-related disease risk factors, including conventional and biologic therapies. A gynecological evaluation and cervical cytology screen were performed. Human papillomavirus detection and genotyping were undertaken by PCR and linear array assay. RESULTS: A total of 148 patients were included, with a mean age and disease duration of 42.5±11.8 years and 9.7±5.3 years, respectively. The prevalence of squamous intraepithelial lesions was 6.8%. The prevalence of human papillomavirus infection was 29%, with human papillomavirus subtype 59 being the most frequent. Patients with human papillomavirus were younger than those without the infection (38.2±11.2 vs. 44.2±11.5 years, respectively; p = 0.05), and patients with the virus had higher daily prednisone doses (12.8±6.8 vs. 9.7±6.7 mg, respectively; p = 0.01) and cumulative glucocorticoid doses (14.2±9.8 vs. 9.7±7.3 g, respectively; p = 0.005) compared with patients without. Patients with human papillomavirus infection more frequently received rituximab than those without (20.9% vs. 8.5%, respectively; p = 0.03). In the multivariate analysis, only the cumulative glucocorticoid dose was associated with human papillomavirus infection. CONCLUSIONS: The cumulative glucocorticoid dose may increase the risk of human papillomavirus infection. Although rituximab administration was more frequent in patients with human papillomavirus infection, no association was found. Screening for human papillomavirus infection is recommended in women with systemic lupus erythematosus

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Biological properties of phenolic compounds from industrial wastes

Phenolic compounds are a large family of metabolites that result from the secondary metabolism of plants. Novel insights about phenolic chemical structure, analytical methods, therapeutic effects, sensory properties, viticultural practices to modify their content and the use of these compounds found in agro-industrial wastes have been gathered in this book. A comprehensive overview on phenolic compounds and neurodegenerative disorders, highlighting their antioxidant, anti-inflammatory properties and their effects on Parkinson’s disease have been compiled. In relation to antioxidant properties, the metabolism and bioavailability of several hydroxycinnamic acids present in coffee have been studied in detail, and also the methods to determine antioxidant capacity have been included. Different strategies in order to improve the extraction and determination of phenolic compounds in a complex matrix by analytical techniques are provided, reporting problems and new analytical solutions. The role of these compounds in color stabilization and also in bitterness and astringency perception has been reported. Moreover, the interactions that take place among no volatile and volatile compounds present in wine affecting sensory perception have been briefly introduced. Furthermore, the use of cover crops in vineyards and their effects on agronomical and enological behavior – particularly, their impact on phenolic compounds – have been highlighted. Finally, the biological properties of phenolic compounds from industrial wastes have been tackled, since they are a promising alternative to transform agro-industrial wastes into a source of natural and healthy compounds. (Imprint: Nova)Fil: Rodriguez Vaquero, Maria Jose. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vallejo, Claudia Veronica. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentin

Berries as Source of Bioactive Compounds

Berries are rich in phenolic compounds. Phenolic compounds, secondary metabolites of plants, exhibit a wide range of biological functions, such as antihypertensive, anti-inflammatory, antioxidant and antimicrobial activities, which are beneficial for human health. Fruits peel, a waste generated during juice elaboration, comprises nearly around 30-40% portion of the fruit. Phenolic compounds are primarily concentrated in the peel portion of berries fruit. Berries and their fruit peels are an economic source of bioactive phenolic compounds having immense possibilities for future investigations related with their utilization as well as recovery. This review summarizes the reported about quantitative and qualitative data of phenolic compositions in different types of berries and its wastes during juice elaboration. Furthermore, biological activities, including antioxidant, antihypertensive, antimicrobial and anti-metabolic disorder effects, have also been discussed.Fil: Vallejo, Claudia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Rodriguez Vaquero, Maria Jose. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentin

Antifungal activity of phenolic extracts on yeast contaminants blueberry juice

Antecedentes: Los cultivos de frutilla y arándanos es una de las principales actividades agrícolas de nuestra provincia. Estudios anteriores evidenciaron que los compuestos fenólicos de vegetales poseen actividades beneficiosas para el consumidor. Objetivos: El objetivo de este trabajo es caracterizar el perfil de compuestos fenólicos de diferentes variedades de arándanos cultivados en Tucumán y evaluar el potencial efecto antimicrobiano del extracto fenólico sobre levaduras aisladas de jugos contaminados. Métodos: Se seleccionaron cuatro variedades de arándanos (Misty, Blue Crisp, Oneal y Milenitim), la fracción fenólica se extrajo utilizando acetato de etilo y fue testeada por cromatografía en capa delgada. Se determinó polifenoles totales utilizando el método de Folin Ciocalteau y el perfil de compuestos fenólicos por HPLC-DAD acoplado a masa. Se realizó el aislamiento de levaduras de jugos de frutillas deteriorados. Las levaduras aisladas se identificaron fenotípica y genotipicamente y se construyó el árbol filogenético. La actividad antifúngica de compuestos fenólicos presentes en arándanos sobre las levaduras aisladas se estudió determinando la viabilidad de levaduras y el efecto sobre la síntesis de ergosterol en presencia de compuestos fenólicos. Resultados: Existen diferencias en la composición fenólica entre las variedades estudiadas, siendo ácido clorogénico el mayoritario.Hanseniaspora osmophila fue una de las levaduras identificadas en jugo deteriorado, los resultados evidenciaron una disminución de su crecimiento en presencia de compuestos fenólicos presentes en arándanos, como así también disminuye la síntesis de ergosterol. Conclusiones: Es la primera vez que se realiza una diferenciación del perfil fenólico entre variedades de arándanos y se reporta actividad antimicrobiana diferencial entre variedades. Como así también, la identificación de levaduras deteriorantes de nuestra región. Estos resultados son fundamentales para buscar nuevas estrategias para el control de estas levaduras deteriorantes.Background: The blueberry culture is one of the principal agricultural activities of our province. Previous studies demonstrate that the phenolic compounds of plants have beneficial activities for the consumer. Objetives: The aims of this work was the characterization of phenolic compounds profile present in different varieties of blueberries cultivated in Tucuman and the evaluation of their potential antifungal activity against yeast isolated from contaminated strawberry juices. Methods: The phenolic fraction of Misty, Blue Crisp, Oneal and Millennium varieties of blueberries were extracted using ethyl acetate and phenolic composition was evaluated using TLC, Folin Ciocalten reagent and HPLC-DAD. The isolation and identification of yeast of deteriorate juice was carried out in YMPG media supplemented with chloramphenicol, phenotypic and genotypic characterization and the phylogenetic tree was constructed. The antifungal activity on isolated yeast was studied by determining the viability and the effect on the synthesis of ergosterol in the presence of phenolic compounds. Results: The phenolic prolife was different between varieties, bur chlorogenic acid was the majority compound in all of them. Hanseniaspora osmophilawas isolated and identified in deteriorated juice. Phenolic compounds present in blueberries decrease the growth of the yeast, as well as the ergosterol synthesis. Conclusions: This is the first study about the polyphenol profile differentiation between blueberries varieties and the antifungal activity of these polyphenolics extracts against spoilage yeasts isolated and identitied of our region. These results are fundamental to find new strategies for the control of this deleterious yeast.Fil: Vallejo, Claudia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Rollan, Graciela Celestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Rodriguez Vaquero, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaXIII Congreso Latinoamericano de Microbiología e Higiene de los AlimentosMedellínColombiaUniversidad de Antioquía. Facultad de Ciencias Farmacéuticas y AlimentariasCorporación Universitaria LasallistaPontificia Universidad Javerian

Antibacterial, antioxidant and antihypertensive properties of polyphenols from argentinean red wines varieties

The aims of this study were to determine the phenolic profile of three Argentinean wine varieties and their antioxidant, antihypertensive and antibacterial activities. Gallic acid, quercetin and rutin were the principal phenolic compounds identified in wines. All wines possess a high antihypertensive and antioxidant activities. Among 3 wines, merlot and malbec possess the higher ferric reducing power and DPPH and ABTS radical scavenging activity. Lower ferric reduced power and radical scavenging values were found in clarified wines. High correlation coefficients were found between phenolic content and antioxidant and antihypertensive capacities, confirming that phenolic compounds are likely the responsible for these activities in wines. The inhibition of biofilm formation and MIC and MBC values of wines polyphenols against E. coli, Pseudomonas aeruginosa, Staphylococcus aureus and L. monocytogenes was demonstrated. The higher antibacterial, antioxidant and ACEI activities of merlot and malbec wines compared with cabernet sauvignon could be related to the higher concentration of individual phenolic compounds, such as quercetin, rutin, kaempferol, caffeic acid and gallic acids in malbec and merlot wines.Fil: Aredes Fernández, Pedro Adrián. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Vallejo, Claudia Veronica. Universidad Nacional de Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Rodriguez Vaquero, Maria Jose. Universidad Nacional de Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentin