Gastric Microbiota and Morphological Changes of the Gastroduodenal Tract Associated with Helicobacter Pylori Infection

© 2016, Springer Science+Business Media New York.Investigation of the microbiota at morphological changes of a gastrointestinal tract. One hundred four patients were examined by cytologic, bacteriological methods, and mass-spectrometry techniques. Studying of the microbial association of stomach has demonstrated the mixed microflora presented by Helicobacter pylori, cocci, fungi Candida, rods, and protozoa. Bacteria of the following species were found: Actinomyces, Arthrobacter, Bacillus, Corynebacterium, Lactobacillus, Neisseria, Pseudomonas, Rothia, Staphylococcus, Streptococcus, and Streptomyces. The microbial flora of esophageal samples was less various (opportunistic bacteria Neisseria, Gemella, and Rothia mucilaginosa were revealed). In group of Helicobacter-positive patients, bacteria were found in fungi Candida, Colletotrichum, and bacterias Thauera and Mycoplasma, while in H.pylori-negative patients, samples have more different species. Intensity of morphological changes (the atrophy, the intestinal metaplasia, and the dysplasia) correlated with a frequency of detection of H. pylori and eukaryotic microbiota (fungi Candida and protozoa)

4.各部門の活動内容 理学療法科

© 2016, Springer Science+Business Media New York.Investigation of the microbiota at morphological changes of a gastrointestinal tract. One hundred four patients were examined by cytologic, bacteriological methods, and mass-spectrometry techniques. Studying of the microbial association of stomach has demonstrated the mixed microflora presented by Helicobacter pylori, cocci, fungi Candida, rods, and protozoa. Bacteria of the following species were found: Actinomyces, Arthrobacter, Bacillus, Corynebacterium, Lactobacillus, Neisseria, Pseudomonas, Rothia, Staphylococcus, Streptococcus, and Streptomyces. The microbial flora of esophageal samples was less various (opportunistic bacteria Neisseria, Gemella, and Rothia mucilaginosa were revealed). In group of Helicobacter-positive patients, bacteria were found in fungi Candida, Colletotrichum, and bacterias Thauera and Mycoplasma, while in H.pylori-negative patients, samples have more different species. Intensity of morphological changes (the atrophy, the intestinal metaplasia, and the dysplasia) correlated with a frequency of detection of H. pylori and eukaryotic microbiota (fungi Candida and protozoa)

Novel type of isoprenoid membrane anchors: an investigation of binding properties with dipalmitoylphosphatidylcholine vesicles

Copyright © 2016 John Wiley & Sons, Ltd.In this work, we present a new type of amphiphilic membrane-anchoring agents that can be easily obtained by the Diels-Alder reaction between terpene myrcene and N-substituted maleimides. The interaction between the compounds and small unilamellar dipalmitoylphosphatidylcholine vesicles was investigated using infrared spectroscopy, microgravimetry, and turbidimetry. The ability of the compounds to embed in the phospholipid membrane was shown to be strongly dependent on the charge of their polar group. The insertion of the compounds studied into the lipid bilayer did not lead to disruption of the dipalmitoylphosphatidylcholine vesicles up to the highest tested drug to lipid molar ratio of 0.5 to 0.6. Low lipid solubilization ability of the compounds as well as their rigid nonplanar structure makes them an interesting alternative to the common membrane-anchoring structural motifs

Novel type of isoprenoid membrane anchors: An investigation of binding properties with dipalmitoylphosphatidylcholine vesicles

© 2016 John Wiley & Sons, Ltd.In this work, we present a new type of amphiphilic membrane-anchoring agents that can be easily obtained by the Diels-Alder reaction between terpene myrcene and N-substituted maleimides. The interaction between the compounds and small unilamellar dipalmitoylphosphatidylcholine vesicles was investigated using infrared spectroscopy, microgravimetry, and turbidimetry. The ability of the compounds to embed in the phospholipid membrane was shown to be strongly dependent on the charge of their polar group. The insertion of the compounds studied into the lipid bilayer did not lead to disruption of the dipalmitoylphosphatidylcholine vesicles up to the highest tested drug to lipid molar ratio of 0.5 to 0.6. Low lipid solubilization ability of the compounds as well as their rigid nonplanar structure makes them an interesting alternative to the common membrane-anchoring structural motifs

Cyclization of 3-(bis(methylthio)methylene)pentane- 2,4-dione to 1-(2-amino-4-methyl-6-( methylthio)pyrimidin-5- yl)ethanone

The 3-(bis(methylthio)methylene)pentane-2,4-dione react with guanidine in the presence of butylate sodium in boiling butanol to give 1-(2-amino-4- methyl-6-( methylthio)pyrimidin-5-yl)ethanone. The structure of the compound were confirmed by physic-chemical methods of analysis.3-(Бис(метилтио)метилен)пентан-2,4-дион реагирует с гуанидином в присутствии бутилата натрия в кипящем бутаноле с образованием 1-(2-амино-4-метил-6-(метилтио)пиримидин-5-ил)этанона. Структура полученного соединения подтверждена физико-химическими методами анализа

High Triglycerides Are Associated with Low Thrombocyte Counts and High VEGF in Nephropathia Epidemica

© 2016 Ekaterina V. Martynova et al.Nephropathia epidemica (NE) is a mild form of hemorrhagic fever with renal syndrome. Several reports have demonstrated a severe alteration in lipoprotein metabolism. However, little is known about changes in circulating lipids in NE. The objectives of this study were to evaluate changes in serum total cholesterol, high density cholesterol (HDCL), and triglycerides. In addition to evaluation of serum cytokine activation associations, changes in lipid profile and cytokine activation were determined for gender, thrombocyte counts, and VEGF. Elevated levels of triglycerides and decreased HDCL were observed in NE, while total cholesterol did not differ from controls. High triglycerides were associated with both the lowest thrombocyte counts and high serum VEGF, as well as a high severity score. Additionally, there were higher levels of triglycerides in male than female NE patients. Low triglycerides were associated with upregulation of IFN-γ and IL-12, suggesting activation of Th1 helper cells. Furthermore, levels of IFN-γ and IL-12 were increased in patients with lower severity scores, suggesting that a Th1 type immune response is playing protective role in NE. These combined data advance the understanding of NE pathogenesis and indicate a role for high triglycerides in disease severity

Критерии психологической оценки родительской позиции в процессе реабилитации детей, перенесших тяжелую травму

Psychological support of the family whose child is in rehabilitation after severe injury is one of the important areas of medical psychologist activities. The psychologist is to involve parents into the rehabilitation process as active participants. Thus, at the initial stage of support, the psychologist’s task is to identify unproductive parental patterns and correct them in a timely manner.The aim of the study is to develop psychological criteria for assessing the parental position in the situation of rehabilitation of children with severe trauma.MATERIAL AND METHODS The trial setting was the Clinical and Research Institute of Emergency Pediatric Surgery and Trauma, Moscow, Russia. 40 parents of children with severe trauma were included in the study. Research instruments were as follows: overt observation of parent-child interaction based on selected evaluation criteria, clinical interviews as well as analysis of homework assignments with the child. To assess parental position in the rehabilitation process, a number of criteria were formed: involvement in the rehabilitation process, attitude towards their child and child’s trauma, attitude towards their child’s independence, attitude towards their parental role as well as the attitude towards the outcome of rehabilitation.RESULTS Types of parental position in the process of the child’s rehabilitation were clarified and updated; its subtypes were defined and characterized. So, according to the criterion “Involvement in the rehabilitation process”, the following distribution by sample was observed: passive (10% of parents), formal (20% of parents), controlling (25% of parents) and productive (45% of parents). According to the criterion “Attitude towards their child” — rejecting (7% of parents), condoning (18% of parents), dominant (35% of parents), accepting (40% of parents). According to the criterion “Attitude towards their child’s trauma” — superficial (10% of parents), defective-directed (32% of parents), positively-directed (18% of parents), adequate (40% of parents). According to the criterion “Attitude towards their child’s independence” — directive (22% of parents), hyper-protective (25% of parents), permissive (18% of parents), democratic (35% of parents). According to the criterion “Attitude towards their parental role” — unformed (17% of parents), dependent (25% of parents), rigid (30% of parents), reflexive (28% of parents). According to the criterion “Attitude towards the outcome of rehabilitation” — amorphous (15% of parents), anxious (15% of parents), categorical (35% of parents), and flexible (35% of parents).CONCLUSIONS The performed psychological assessment of parental position in the process of their child’ rehabilitation after severe injury has revealed important intra-group differences, indicating that a significant part of parents need psychological help, since they cannot independently come to productive forms of interaction with their child in the process of his rehabilitation.АКТУАЛЬНОСТЬ Психологическое сопровождение семьи в ходе реабилитации ребенка после тяжелой травмы является одним из важных направлений работы медицинского психолога. Данная работа предполагает включение родителя в этот процесс как активного участника. В этом контексте на первоначальном этапе сопровождения стоит задача оценки родительской позиции с целью выявления ее непродуктивных характеристик и их своевременной коррекции.ЦЕЛЬ ИССЛЕДОВАНИЯ Разработка психологических критериев оценки родительской позиции в ситуации реабилитации детей с тяжелой травмой.МАТЕРИАЛ И МЕТОДЫ Исследование проводилось на базе НИИ неотложной детской хирургии и травматологии, г. Москва. В исследовании принимали участие 40 родителей детей с тяжелой травмой.Использованы включенное наблюдение за взаимодействием родителя с ребенком на основе выделенных критериев оценивания, клиническая беседа, анализ заданий по выполнению домашней работы с ребенком. Для оценки родительской позиции в процессе реабилитации был сформирован ряд критериев: включенность в процесс реабилитации, отношение к ребенку, ориентировка в травме ребенка, отношение к самостоятельности ребенка, родительской роли и исходу реабилитации.РЕЗУЛЬТАТЫ Были уточнены и дополнены сведения о типах родительской позиции в процессе реабилитации ребенка; выделены и охарактеризованы их подтипы. Так, по критерию «Включенность родителя в реабилитацию» наблюдалось следующее распределение по выборке: пассивная (10% родителей), формальная (20% родителей), контролирующая (25% родителей) и продуктивная (45% родителей). По критерию «Отношение к собственному ребенку» — отвергающее (7% родителей), потворствующее (18% родителей), доминантное (35% родителей), принимающее (40% родителей). По критерию «Ориентировка в травме ребенка» — поверхностная (10% родителей), дефективно-направленная (32% родителей), позитивно-направленная (18% родителей), адекватная (40% родителей). По критерию «Отношение к самостоятельности ребенка» — директивное (22% родителей), гиперопекающее (25% родителей), попустительское (18% родителей), демократичное (35% родителей). По критерию «Отношение к родительской роли» — неоформленная (17% родителей), зависимая (25% родителей), ригидная (30% родителей), рефлексивная (28% родителей). По критерию «Отношение к исходу реабилитации» — аморфное (15% родителей), тревожное (15% родителей), категоричное (35% родителей) и гибкое (35% родителей).ЗАКЛЮЧЕНИЕ Родительская позиция в процессе психологической работы в условиях реабилитационного отделения для детей, перенесших тяжелую травму, имеет существенные внутригрупповые различия, указывающие на то, что значительной части родителей необходима психологическая помощь, так как им не удается самостоятельно прийти к продуктивным формам взаимодействия со своим ребенком в процессе его реабилитации

Current status of nuclear cardiology in the Russian Federation

The article is devoted to the analysis of the current status of nuclear cardiology in the Russian Federation. The data on the number of facilities performing radionuclide investigations for the diagnosis and monitoring of the treatment of cardiovascular diseases, their staffing and equipment are given. The statistics of the conducted nuclear cardiology tests for 2018-2020 are given, as well as their methods, features and diagnostic significance are described

‘NADEZHDA’ IS A NEW VARIETY OF SOFT WINTER WHEAT ADAPTED TO THE CONDITIONS OF THE MIDDLE POVOLZHIE

Due to the creation and introduction of the varieties adapted to the conditions of the Republic of Tatarstan, the areas of winter wheat cultivation increased from 59.300 ha to 339.700 ha during the last 15 years. The staff of the institute developed such winter wheat varieties as ‘Kazanskaya 285’, ‘Kazanskaya 560’ and ‘Nadezhda’ with the stronger adaptive characteristics and a sustained grain quality. The article describes a new soft winter wheat variety ‘Nadezhda’. The variety was created with the method of intraspecific hybridization with the following selection from the hybrids: ‘Mironovskaya 808’/’Meshinskaya 2’/’Kazanskaya 285’. The variety is Lyutestsens, a middle maturing cultivar. The average productivity of the variety during the years of study was 3.5 t/ha. the cultivar showed the productivity increase of 0.5 t/ha compared with the standard variety ’Kazanskaya 285’. The distinctive feature of the variety is the synchronization of the stem formation, which weakens the competition among the first and following stems. The variety ‘Nadezhda’ possesses a powerful autumn tillering that allows sowing it with the norm of 4.5-5.0 ml of germinating seeds per hectare. The cultivar possesses resistance to snow mould, fusariosis and tolerance to drought. It’s valuable wheat with large and uniform kernels. The variety has been approved to use in the Middle-Volzhsky region of the Russian Federation

The new variety of winter soft wheat for the Middle Povolzhye ‘DARINA’

The article gives the description of winter soft wheat variety ‘Darina’. The variety has been developed by intraspecific hybridization with the further individual selection from the hybrid population, i.e. winter variety ‘Omskaya’/’Kazanskaya 560’/‘Kazanskaya 285’. The variety of wheat is erythrospermum. The cultivar is of high productivity with the average yield of 4.89 t/ha during the years of testing, that is on 0.95 t/ha more than the productivity of the standard variety. The cultivar produces stable yields, if it is sown in black or seed fallow land with the use of conventional agro technology. Fertilizing of 1 kg of active nitrogen increases grain productivity of the cultivar on 10-12kg, that proves its high responsiveness on mineral fertilizing. The variety is middle maturing, resistant to snow mold and seeding blight (fusarium). The variety has been listed into the List of valuable grain wheat. It has been recommended for the Middle Volga region of the Russian Federation