The Most Poetic Subject in the World

THE MOST POETIC SUBJECT IN THE WORLD: OBSERVATIONS ON DEATH, (BEAUTIFUL) WOMEN AND REPRESENTATION IN BLADE RUNNER WESTERN culture has a long tradition of associating death with femininity and sex. Whether it is Snow White or Bluebeard's wives, Clarissa or Madame Bovary, Carmen or Madame Butterfly, representations of dead and dying women fascinate and disturb. A Greek motto from Palladas states that "Every woman is as bitter as gall; but she has two good moments, one in bed, the other at her death" (quoted by Mérimée, 181). According to Edgar Allan Poe, "the death of a beautiful woman is, unquestionably, the most poetic subject in the world" (19). Thomas de Quincey acknowledges ". . . with the love that burns in depths of admiration . . . that you [sister woman] can do one thing as well as the best of us men -- a greater thing than even..

EFFECT OF SODIUM STEARATE (NAST) ON AIR FRESHENER TRANSPARENCY

Sodium Stearate (NaSt) is produced in Stabilchem (M) Sdn. Bhd. via the fusion process between palm-based Fatty Acid (FA) and Sodium Hydroxide (NaOH). NaSt has a multitude of applications ranging from lubricating agents in wire drawing to emulsifying agents in the food industry. NaSt is also beginning to be used as a gelling agent in gel-based air fresheners. It is required that the NaSt used in the air freshener produce a translucent gel. In order to fulfill this industrial requirement, NaSt has to be tested for its performance in gel-based air fresheners. To do this, samples of NaSt with different chemical and physical properties are used to produce air fresheners. The air fresheners produced are then measured for transparency. The object of this project is to study the effect of chemical and physical properties of NaSt on gel air freshener transparency and to recommend the optimum NaSt parameters to be used in air fresheners. For this project, only the NaSt produced by Stabilchem (M) Sdn. Bhd. were used. To measure the gel transparency, a spectrophotometer called Hunter Lab Meter was used. Only three parameters were studied in this project, namely the effect of Free Fatty Acid (FFA) value and moisture content of the NaSt as well as the heating temperature when producing the air freshener. All the sample analysis laboratory test methods are that as stated in Stabilchem's Quality Control department. The air freshener formulation used was provided by a client of Stabilchem under confidentiality and trust. Therefore, the detailed formulation is not provided in this report. Among the components in the air freshener are D-Limonene, NaSt, Nonyl phenol and ethanol. After conducting this investigative project, it was found that gel air freshener transparency decreases slightly with increasing FFA values. The moisture content of NaSt has insignificant effect on gel transparency. Last but not least, gel transparency decreases as heating temperature is increased. From these findings, it can be concluded that Stabilchem's NaSt are capable of producing air freshener gels with satisfactory clarity as long as its chemical and physical properties are within the specifications set by the QC department. To ensure that the gel producedis always clear, the air freshener should be produced in a clean and dry area. Additives such as fragrance and colour can cause cloudiness. Therefore, compatible colouring and fragrance should be used in correct amounts to preserve the clarity ofthe air freshener gel

In silico modelling of hormone response elements

BACKGROUND: An important step in understanding the conditions that specify gene expression is the recognition of gene regulatory elements. Due to high diversity of different types of transcription factors and their DNA binding preferences, it is a challenging problem to establish an accurate model for recognition of functional regulatory elements in promoters of eukaryotic genes. RESULTS: We present a method for precise prediction of a large group of transcription factor binding sites – steroid hormone response elements. We use a large training set of experimentally confirmed steroid hormone response elements, and adapt a sequence-based statistic method of position weight matrix, for identification of the binding sites in the query sequences. To estimate the accuracy level, a table of correspondence of sensitivity vs. specificity values is constructed from a number of independent tests. Furthermore, feed-forward neural network is used for cross-verification of the predicted response elements on genomic sequences. CONCLUSION: The proposed method demonstrates high accuracy level, and therefore can be used for prediction of hormone response elements de novo. Experimental results support our analysis by showing significant improvement of the proposed method over previous HRE recognition methods

Why do small business taxpayers stay with their practitioners? Trust, competence and aggressive advice

This article draws upon self-report survey data to examine the commitment of 181 small business taxpayers to their tax practitioners. Using hierarchical regression modelling, commitment was associated with a local chartered accountant; having a letter of engagement; receiving cautious, competent and aggressive advice (once expectations were controlled) and believing the tax practitioner trustworthy. Trust was related to receiving competent and cautious advice. Receiving aggressive advice contributed to commitment independently of being considered professionally competent and trustworthy

Tetratricopeptide repeat domain 9A is an interacting protein for tropomyosin Tm5NM-1

<p>Abstract</p> <p>Background</p> <p>Tetratricopeptide repeat domain 9A (TTC9A) protein is a recently identified protein which contains three tetratricopeptide repeats (TPRs) on its C-terminus. In our previous studies, we have shown that TTC9A was a hormonally-regulated gene in breast cancer cells. In this study, we found that TTC9A was over-expressed in breast cancer tissues compared with the adjacent controls (P < 0.00001), suggesting it might be involved in the breast cancer development process. The aim of the current study was to further elucidate the function of TTC9A.</p> <p>Methods</p> <p>Breast samples from 25 patients including the malignant breast tissues and the adjacent normal tissues were processed for Southern blot analysis. Yeast-two-hybrid assay, GST pull-down assay and co-immunoprecipitation were used to identify and verify the interaction between TTC9A and other proteins.</p> <p>Results</p> <p>Tropomyosin Tm5NM-1 was identified as one of the TTC9A partner proteins. The interaction between TTC9A and Tm5NM-1 was further confirmed by GST pull-down assay and co-immunoprecipitation in mammalian cells. TTC9A domains required for the interaction were also characterized in this study. The results suggested that the first TPR domain and the linker fragment between the first two TPR domains of TTC9A were important for the interaction with Tm5NM-1 and the second and the third TPR might play an inhibitory role.</p> <p>Conclusion</p> <p>Since the primary function of tropomyosin is to stabilize actin filament, its interaction with TTC9A may play a role in cell shape and motility. In our previous results, we have found that progesterone-induced TTC9A expression was associated with increased cell motility and cell spreading. We speculate that TTC9A acts as a chaperone protein to facilitate the function of tropomyosins in stabilizing microfilament and it may play a role in cancer cell invasion and metastasis.</p

The Rad4TopBP1 ATR-Activation domain functions in G1/S phase in a chromatin-dependent manner

DNA damage checkpoint activation can be subdivided in two steps: initial activation and signal amplification. The events distinguishing these two phases and their genetic determinants remain obscure. TopBP1, a mediator protein containing multiple BRCT domains, binds to and activates the ATR/ATRIP complex through its ATR-Activation Domain (AAD). We show that Schizosaccharomyces pombe Rad4TopBP1 AAD–defective strains are DNA damage sensitive during G1/S-phase, but not during G2. Using lacO-LacI tethering, we developed a DNA damage–independent assay for checkpoint activation that is Rad4TopBP1 AAD–dependent. In this assay, checkpoint activation requires histone H2A phosphorylation, the interaction between TopBP1 and the 9-1-1 complex, and is mediated by the phospho-binding activity of Crb253BP1. Consistent with a model where Rad4TopBP1 AAD–dependent checkpoint activation is ssDNA/RPA–independent and functions to amplify otherwise weak checkpoint signals, we demonstrate that the Rad4TopBP1 AAD is important for Chk1 phosphorylation when resection is limited in G2 by ablation of the resecting nuclease, Exo1. We also show that the Rad4TopBP1 AAD acts additively with a Rad9 AAD in G1/S phase but not G2. We propose that AAD–dependent Rad3ATR checkpoint amplification is particularly important when DNA resection is limiting. In S. pombe, this manifests in G1/S phase and relies on protein– chromatin interactions

DEVELOPMENT OF SILVER CATALYSTS FOR THE HETEROCYCLIZATION REACTIONS OF ALKYNES

Ph.DNUS-ICL JOINT PH.D. (FoS

Diversifying Large-Scale Participatory Science: The Efficacy of Engagement through Facilitator Organizations

Large-scale, scientist-led, participatory science (citizen science) projects often engage participants who are primarily white, wealthy, and well-educated. Calls to diversify contributory projects are increasingly common, but little research has evaluated the efficacy of suggested strategies for diversification. We engaged participants in Crowd the Tap through facilitator organizations like historically Black colleges and universities (HBCUs), predominantly white institutions, high school science classrooms, and corporate volunteer programs. Crowd the Tap is a contributory project focused on identifying and addressing lead (Pb) contamination in household drinking water in the United States. We investigated how participant diversity with respects to race, ethnicity, and homeownership (a proxy for income) differed between participation facilitated through a partner organization and unfacilitated participation in which participants came to the project independently. We were also interested in which facilitators were most effective at increasing participant diversity. White and wealthy participants were overrepresented in unfacilitated participation. Facilitation helped increase engagement of people of color, especially Black and lower-income households. High schools were particularly effective at engaging Hispanic or Latinx participants, and HBCUs were important for engaging Black households. Ultimately, our results suggest that engagement through facilitator organizations may be an effective means of engaging diverse participants in large-scale projects. Our results have important implications for the field of participatory science as we seek to identify evidence-based strategies for diversifying project participants

Phosphorylation-dependent assembly and coordination of the DNA damage checkpoint apparatus by Rad4TopBP1

The BRCT-domain protein Rad4(TopBP1) facilitates activation of the DNA damage checkpoint in Schizosaccharomyces pombe by physically coupling the Rad9-Rad1-Hus1 clamp, the Rad3(ATR) -Rad26(ATRIP) kinase complex, and the Crb2(53BP1) mediator. We have now determined crystal structures of the BRCT repeats of Rad4(TopBP1), revealing a distinctive domain architecture, and characterized their phosphorylation-dependent interactions with Rad9 and Crb2(53BP1). We identify a cluster of phosphorylation sites in the N-terminal region of Crb2(53BP1) that mediate interaction with Rad4(TopBP1) and reveal a hierarchical phosphorylation mechanism in which phosphorylation of Crb2(53BP1) residues Thr215 and Thr235 promotes phosphorylation of the noncanonical Thr187 site by scaffolding cyclin-dependent kinase (CDK) recruitment. Finally, we show that the simultaneous interaction of a single Rad4(TopBP1) molecule with both Thr187 phosphorylation sites in a Crb2(53BP1) dimer is essential for establishing the DNA damage checkpoint