Penentuan Besar Ganti Rugi Pengadaan Tanah Untuk Kepentingan Umum Berdasarkan Undang-undang Nomor 2 Tahun 2012 Tentang Pengadaan Tanah Bagi Pembangunan Untuk Kepentingan Umum

Tujuan dilakukannya penelitian ini adalah untuk mengetahui bagaimana kebijakan mengenai aturan hukum pengadaan tanah bagi pembangunan untuk kepentingan umum dan bagaimana penentuan besar ganti rugi terhadap pengadaan tanah bagi pembangunan untuk kepentingan umum dalam Undang-Undang Nomor 2 Tahun 2012. Dengan menggunakan metode penelitian yuridis normatif, disimpulkan: 1. Aturan atau regulasi pengadaan tanah bagi pembangunan untuk kepentingan umum dapat dikelompokkan baik dalam Perundang-undangan, Keputusan Presiden, Instruksi Presiden, Peraturan Menteri, Peraturan Kepala Badan Pertanahan Nasonal. 2. Mencermati mekanisme penentuan besaran maupun bentuk Ganti Kerugian dalam UU No. 2 Tahun 2012 serta dasar acuan yang digunakan dalam menentukan besaran maupun bentuk Ganti Kerugian yang diatur dalam Perpres 71/2012. sebenarnya tidak jauh berbeda dengan ketentuan mengenai mekanisme penentuan besaran maupun bentuk Ganti Kerugian serta dasar acuan yang digunakan dalam menentukan besaran maupun bentuk Ganti Kerugian yang diatur PerKa BPN 03/2007. Baik dalam UU No 2/2012, Perpres 71/2012 maupun dalam PerKa BPN 05/2012, yang dijadikan dasar atau acuan dalam menilai besaran nilai ganti kerugian atau harga tanah hanya pada NJOP, nilai jual bangunan, nilai jual tanaman bukan pada nilai jual berdasarkan harga pasar seperti yang selama ini diinginkan oleh masyarakat yang terkena pengadaan tanah

Time-lapse electrical resistivity anomalies due to contaminant transport around landfills

The extent of landfill leachate can be delineated by geo-electrical imaging as a response to the varying electrical resistivity in the contaminated area. This research was based on a combination of hydrogeological numerical simulation followed by geophysical forward and inversion modeling performed to evaluate the migration of a contaminant plume from a landfill. As a first step, groundwater flow and contaminant transport was simulated using the finite elements numerical modeling software FEFLOW. The extent of the contaminant plume was acquired through a hydrogeological model depicting the distributions of leachate concentration in the system. Next, based on the empirical relationship between the concentration and electrical conductivity of the leachate in the porous media, the corresponding geo-electrical structure was derived from the hydrogeological model. Finally, forward and inversion computations of geo-electrical anomalies were performed using the finite difference numerical modeling software DCIP2D/DCIP3D. The image obtained by geophysical inversion of the electric data was expected to be consistent with the initial hydrogeological model, as described by the distribution of leachate concentration. Numerical case studies were conducted for various geological conditions, hydraulic parameters and electrode arrays, from which conclusions were drawn regarding the suitability of the methodology to assess simple to more complex geo-electrical models. Thus, optimal mapping and monitoring configurations were determined

Beam model of Doppler backscattering

We use beam tracing -- implemented with a newly-written code, Scotty -- and the reciprocity theorem to derive a model for the linear backscattered power of the Doppler Backscattering (DBS) diagnostic. Our model works for both the O-mode and X-mode in tokamak geometry (and certain regimes of stellarators). We present the analytical derivation of our model and its implications on the DBS signal localisation and the wavenumber resolution. To determine these two quantities, we find that it is the curvature of the field lines and the magnetic shear that are important, rather than the curvature of the cut-off surface. We also provide an explicit formula for the hitherto poorly-understood quantitative effect of the mismatch angle. Consequently, one can use this model to correct for the attenuation due to mismatch, avoiding the need for empirical optimisation. This is especially important in spherical tokamaks, since the magnetic pitch angle is large and varies both spatially and temporally.Comment: This is the version that passed peer review. No major changes, but many improvements to writing styl

Structural Color 3D Printing By Shrinking Photonic Crystals

The rings, spots and stripes found on some butterflies, Pachyrhynchus weevils, and many chameleons are notable examples of natural organisms employing photonic crystals to produce colorful patterns. Despite advances in nanotechnology, we still lack the ability to print arbitrary colors and shapes in all three dimensions at this microscopic length scale. Commercial nanoscale 3D printers based on two-photon polymerization are incapable of patterning photonic crystal structures with the requisite ~300 nm lattice constant to achieve photonic stopbands/ bandgaps in the visible spectrum and generate colors. Here, we introduce a means to produce 3D-printed photonic crystals with a 5x reduction in lattice constants (periodicity as small as 280 nm), achieving sub-100-nm features with a full range of colors. The reliability of this process enables us to engineer the bandstructures of woodpile photonic crystals that match experiments, showing that observed colors can be attributed to either slow light modes or stopbands. With these lattice structures as 3D color volumetric elements (voxels), we printed 3D microscopic scale objects, including the first multi-color microscopic model of the Eiffel Tower measuring only 39-microns tall with a color pixel size of 1.45 microns. The technology to print 3D structures in color at the microscopic scale promises the direct patterning and integration of spectrally selective devices, such as photonic crystal-based color filters, onto free-form optical elements and curved surfaces

Grapevine leafroll-associated virus 3.

Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if intervention strategies are not implemented. Several viruses from the family Closteroviridae are associated with GLD. However, Grapevine leafroll-associated virus 3 (GLRaV-3), the type species for the genus Ampelovirus, is regarded as the most important causative agent. Here we provide a general overview on various aspects of GLRaV-3, with an emphasis on the latest advances in the characterization of the genome. The full genome of several isolates have recently been sequenced and annotated, revealing the existence of several genetic variants. The classification of these variants, based on their genome sequence, will be discussed and a guideline is presented to facilitate future comparative studies. The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs. The latest nucleotide sequence based molecular diagnostic techniques were shown to be more sensitive than conventional serological assays and although ELISA is not as sensitive it remains valuable for high-throughput screening and complementary to molecular diagnostics. The application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction. Next-generation sequencing data can provide information regarding disease complexes, variants of viral species, and abundance of particular viruses. This information can be used to develop more accurate diagnostic assays. Reliable virus screening in support of robust grapevine certification programs remains the cornerstone of GLD management

Combining biochar with low rate of chemical fertiliser boosts maize biomass yield, regardless of tillage system, under humid conditions

Biochar application to soils increases biomass and crop yields, especially with rates higher than 100 t ha−1. Yet, there is limited knowledge on the combined effect of biochar and chemical fertiliser under different tillage systems. The objective of this study is to investigate the effect of maize-cob biochar (BC) (rates of 5 and 10 t ha−1) combined with chemical fertiliser micro-dosing (MD) at a rate of 25% of the recommended quantity on total shoot dry matter (DM) and plant height of maize cultivated under flat (F) and tied-ridge (R) practices during a humid season in Tanzania. The results indicate that combining 5 t ha−1 BC with 25% MD increases DM at harvest by 83% (4.16 t ha−1) compared to the control (2.27 t ha−1) and was in the same range as the DM obtained from the treatment with the recommended fertiliser rate (100% FD). The treatments with single applications of 25% MD, 5 t ha−1 BC, and 10 t ha−1 BC only tended to exceed the control of DM yield. Therefore, we recommend that small-scale farmers aiming at DM for livestock or grain yield with limited access to chemical fertilisers to combine biochar with 25% MD, rather than applying biochar or low chemical fertiliser rates alone

Activation of Cytochrome C Peroxidase Function Through Coordinated Foldon Loop Dynamics upon Interaction with Anionic Lipids

Cardiolipin (CL) is a mitochondrial anionic lipid that plays important roles in the regulation and signaling of mitochondrial apoptosis. CL peroxidation catalyzed by the assembly of CL-cytochrome c (cyt c) complexes at the inner mitochondrial membrane is a critical checkpoint. The structural changes in the protein, associated with peroxidase activation by CL and different anionic lipids, are not known at a molecular level. To better understand these peripheral protein-lipid interactions, we compare how phosphatidylglycerol (PG) and CL lipids trigger cyt c peroxidase activation, and correlate functional differences to structural and motional changes in membrane-associated cyt c. Structural and motional studies of the bound protein are enabled by magic angle spinning solid state NMR spectroscopy, while lipid peroxidase activity is assayed by mass spectrometry. PG binding results in a surface-bound state that preserves a nativelike fold, which nonetheless allows for significant peroxidase activity, though at a lower level than binding its native substrate CL. Lipid-specific differences in peroxidase activation are found to correlate to corresponding differences in lipid-induced protein mobility, affecting specific protein segments. The dynamics of omega loops C and D are upregulated by CL binding, in a way that is remarkably controlled by the protein:lipid stoichiometry. In contrast to complete chemical denaturation, membrane-induced protein destabilization reflects a destabilization of select cyt c foldons, while the energetically most stable helices are preserved. Our studies illuminate the interplay of protein and lipid dynamics in the creation of lipid peroxidase-active proteolipid complexes implicated in early stages of mitochondrial apoptosis

Surface-Binding to Cardiolipin Nanodomains Triggers Cytochrome c Pro-apoptotic Peroxidase Activity via Localized Dynamics

The peroxidation of cardiolipins by reactive oxygen species, which is regulated and enhanced by cytochrome c (cyt c), is a critical signaling event in mitochondrial apoptosis. We probe the molecular underpinnings of this mitochondrial death signal through structural and functional studies of horse heart cyt c binding to mixed-lipid membranes containing cardiolipin with mono- and polyunsaturated acyl chains. Lipidomics reveal the selective oxidation of polyunsaturated fatty acid (PUFA) cardiolipin (CL), while multidimensional solid-state NMR probes the structure and dynamics of the membrane and the peripherally bound protein. The hydrophilic milieu at the membrane interface stabilizes a native-like fold, but also leads to localized flexibility at the membrane-interacting protein face. PUFA CL acts as both a preferred substrate and a dynamic regulator by affecting the dynamics of the cyt c N70-I85 Ω loop, which covers the heme cavity

Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene.

The RNA genome of tobacco etch potyvirus (TEV) was engineered to express bacterial 13-glucuronidase (GUS) fused to the virus helper component proteinase (HC-Pro). It was shown previously that prolonged periods (-1 month) of TEV-GUS propagation in plants resulted in the appearance of spontaneous deletion variants. Nine deletion mutants were identified by nucleotide sequence analysis of 40 cDNA clones obtained after polymerase chain reaction amplification. The mutants were missing between 1,741 and 2,074 nucleotides from TEV-GUS, including the sequences coding for most of GUS and the N-terminal region of HC-Pro. This region of HC-Pro contains determinants involved in helper component activity during aphid transmission, as well as a highly conserved series of cysteine residues. The deletion variants were shown to replicate and move systemically without the aid of a helper virus. Infectious viruses harboring the two largest HC-Pro deletions (termed TEV-2del and TEV-7del) were reconstructed by subcloning the corresponding mutated regions into full-length DNA copies of the TEV genome. Characterization of these and additional variants derived by site-directed mutagenesis demonstrated that deletion of sequences coding for the HC-Pro N-terminal domain had a negative effect on accumulation of viral RNA and coat protein. The TEV-2del variant possessed an aphid-nontransmissible phenotype that could be rescued partially by prefeeding of aphids on active HC-Pro from another potyvirus. These data suggest that the N-terminal domain of HC-Pro or its coding sequenc