The CIARD RING, an infrastructure for interoperability of agricultural research information services

Creating integrated information services in agriculture giving access and adding value to information residing in distributed sources remains a major challenge. In distributed architectures, value added services by definition interface several information sources / services. Therefore value added services cannot be built without an awareness of what others have done: which sources are available, how to tap into them, how to exploit their semantics. The Coherence in Information for Agricultural Research for Development (CIARD) Routemap to Information Nodes and Gateways (RING) is a portal offering an interlinked registry of existing information services in agriculture. The CIARD RING covers both information services and sources: in nowadays information architectures, the distinction between the two is very fluid. In the RING, the definition of "service" includes any form of providing information from one server instance (website, mail server, web services, XML archive...) to many clients (browsers, email clients, news readers, harvesters...) The services registered in the RING are described in details and categorized according to criteria that are relevant to the use of the service and its interoperability. The RING categorizes and interlinks the featured services according to criteria such as: standards adopted, vocabulary used, technology used, protocols implemented, level of interoperability etc. In addition, it features detailed instructions on how the registered services can be "interoperated". The vision is that the RING will become the common global technical platform for the community of agricultural information professionals for accessing, sharing and exchanging information through web services. This paper describes how the RING provides an infrastructure for enhancing interoperability of information sources and thus paves the way towards better accessibility of information through value-added and better targeted services

Review of codes of conduct, voluntary guidelines and principles relevant for farm data sharing

Codes of conduct, voluntary guidelines, sets of principles on how to transparently govern farm data are a recent thing. While laws and regulations that govern personal data are becoming more and more common, legislation still does not cover data flows in many industries where different actors in the value chain need to share data and at the same time protect all involved from the risks of data sharing. Data in these value chains is currently governed through private data contracts or licensing agreements, which are normally very complex and on which data producers have very little negotiating power. Codes of conduct have started to emerge to fill the legislative void and to set common standards for data sharing contracts: codes provide principles that the signatories/subscribers/members agree to apply in their contracts

The CIARD RING, an infrastructure for interoperability of agricultural research information services

Creating integrated information services in agriculture giving access and adding value to information residing in distributed sources remains a major challenge. In distributed architectures, value added services by definition interface several information sources / services. Therefore value added services cannot be built without an awareness of what others have done: which sources are available, how to tap into them, how to exploit their semantics. The Coherence in Information for Agricultural Research for Development (CIARD) Routemap to Information Nodes and Gateways (RING) is a portal offering an interlinked registry of existing information services in agriculture. The CIARD RING covers both information services and sources: in nowadays information architectures, the distinction between the two is very fluid. In the RING, the definition of "service" includes any form of providing information from one server instance (website, mail server, web services, XML archive...) to many clients (browsers, email clients, news readers, harvesters...) The services registered in the RING are described in details and categorized according to criteria that are relevant to the use of the service and its interoperability. The RING categorizes and interlinks the featured services according to criteria such as: standards adopted, vocabulary used, technology used, protocols implemented, level of interoperability etc. In addition, it features detailed instructions on how the registered services can be "interoperated". The vision is that the RING will become the common global technical platform for the community of agricultural information professionals for accessing, sharing and exchanging information through web services. This paper describes how the RING provides an infrastructure for enhancing interoperability of information sources and thus paves the way towards better accessibility of information through value-added and better targeted services

Guided implant surgery and sinus lift in severely resorbed maxillae: A retrospective clinical study with up to 10 years of follow-up

Objectives In the posterior maxilla, due to the presence of maxillary sinus, residual bone height lower than 3mm is a critical factor that can affect implant stability and survival. The use of guided surgery may facilitate the surgical procedures and the implant insertion in case of severely resorbed maxillae. Moreover, it may have beneficial effects on the long-term survival and success of implant-supported restorations. This study aimed to evaluate implant supported restorations on severely resorbed maxilla (<3 mm) after sinus lift with collagenated xenograft and guided surgery. Methods Forty-three patients with need for implant rehabilitation and residual bone height between 1 and 3 mm were recruited. Surgical and prosthetical aspects were planned following digital approach with the use of Realguide 5.0 (3diemme, Varese, Italy). Lateral window sinus lift was performed and implants were placed simultaneously to the augmentation procedure with a tooth-supported pilot drill surgical template. A pre-hydrated collagenated porcine bone matrix was adopted as regenerative material. Computer-aided-design/computer-aided-manufacturing (CAD/CAM) restorations were delivered after six months of healing. Milled titanium chamfer abutments with CAD/CAM crowns were used. Bone height at implant site level was measured using an image software analysis applied to the pre- and post-surgical radiographs and at the follow-up. Biological and technical complications were recorded during all the follow-up periods. Results Fifty-four sinus were treated. After a mean follow-up time of 5.11 years (SD: 2.47), no implants were lost nor showed signs of disease. The mean pristine bone height was 2.07 mm (SD: 075). At the final evaluation the augmented sinus height was 12.83 mm (SD: 1.23). Two cases experienced minor perforation of the membrane, while five patients developed minimal post-operative complications, completely resolved with pharmacologic therapy. No mid-term biological complications were experienced by the patients. No cases experienced peri-implant mucositis and peri-implantitis during the whole follow-up period. Four patients (7.4%) faced an unscrewing of the prosthesis. Conclusions The present study showed the efficacy in the mid-term of the digital planning and the guided surgery in restoring severely resorbed posterior maxilla with dental implants

A prospective case series on surgical treatment of circumferential and semi-circumferential defects due to peri-implantitis

Different surgical treatment strategies for peri-implantitis with the use of graft material and membranes have been suggested without any longitudinal remarkable success rate. The present preliminary study was aimed to analyze a new clinical approach based on the disinfection of the implant connection, the disinfection of the implant surface and GBR approach in the treatment of circumferential and semi-circumferential bony defect resulting from peri-implantitis. Six consecutive patients were selected for the present study. After removal of factors that could potentially influence peri-implant pathology, the prosthetic rehabilitation was always removed and a full thickness flap was elevated to allow access to the peri-implant defect and the exposed implant surface. Once the defect was degranulated and the implant surface cleaned, a mixture (50:50) of autogenous bone and allograft was used. Guided bone regeneration technique using membranes was adopted and the flaps were closed for a submerged healing. Six months thereafter, a new re-opening procedure was performed and cleaned superstructures and crowns were repositioned. Patients were followed for one year thereafter and recalled for a customized oral hygiene every three months. Radiological and periodontal analysis was performed before surgery and every six months. The studied procedure was associated with a pronounced increase in REC and CAL with stable peri-implant conditions at 6 and 12 months. PI, BOP and PD values were significantly reduced both at 6 and 12 months. At 12 months, a mean PD gain of 4.5 mm and a bone loss reduction of 5.1 mm was obtained. Within the limitation of the present preliminary study, the proposed technique might represent a promising result for treatment of circumferential and semi-circumferential bone defects around implants affected by peri-implantitis

The effect of pharmacological treatment on ADMA in patients with heart failure.

Asymmetric dimethylarginine (ADMA) plays a crucial role in the arginine-nitric oxide (NO) pathway. NO plays an important role in controlling vascular tone and regulates the contractile properties of cardiac myocytes. The purpose of this study was to investigate the effect of pharmacological treatment on asymmetrical dimethylarginine (ADMA) plasma levels in patients with acute congestive heart failure (HF). Patients with symptomatic acute congestive HF (NYHA Class III-IV) and impaired left ventricular (LV) function (ejection fraction less than 40 percent) were included in the study. ADMA and SDMA concentrations were assessed before and after pharmacological treatment in 18 critically ill patients on the intensive care unit by high performance liquid chromatography. All patients received a complete pharmacological treatment (diuretics, digoxin, ACE-inhibitors or angiotensin receptor blockers, and nitroglicerin) for the treatment of acute congestive HF. ADMA plasma levels of critically ill patients were significantly higher after pharmacological treatment respect baseline values (pre-treatment). In critically ill patients with acute congestive HF acute renal impairment function and the modulation of NOS determine plasma ADMA/SDMA levels after therapy

Journey on VX-809-Based Hybrid Derivatives towards Drug-like F508del-CFTR Correctors: From Molecular Modeling to Chemical Synthesis and Biological Assays

open12Cystic fibrosis (CF) is a genetic disease affecting the lungs and pancreas and causing progressive damage. CF is caused by mutations abolishing the function of CFTR, a protein whose role is chloride's mobilization in the epithelial cells of various organs. Recently a therapy focused on small molecules has been chosen as a main approach to contrast CF, designing and synthesizing compounds acting as misfolding (correctors) or defective channel gating (potentiators). Multi-drug therapies have been tested with different combinations of the two series of compounds. Previously, we designed and characterized two series of correctors, namely, hybrids, which were conceived including the aminoarylthiazole (AAT) core, merged with the benzodioxole carboxamide moiety featured by VX-809. In this paper, we herein proceeded with molecular modeling studies guiding the design of a new third series of hybrids, featuring structural variations at the thiazole moiety and modifications on position 4. These derivatives were tested in different assays including a YFP functional assay on models F508del-CFTR CFBE41o-cells, alone and in combination with VX-445, and by using electrophysiological techniques on human primary bronchial epithelia to demonstrate their F508del-CFTR corrector ability. This study is aimed (i) at identifying three molecules (9b, 9g, and 9j), useful as novel CFTR correctors with a good efficacy in rescuing the defect of F508del-CFTR; and (ii) at providing useful information to complete the structure-activity study within all the three series of hybrids as possible CFTR correctors, supporting the development of pharmacophore modelling studies, taking into account all the three series of hybrids. Finally, in silico evaluation of the hybrids pharmacokinetic (PK) properties contributed to highlight hybrid developability as drug-like correctors.openParodi, Alice; Righetti, Giada; Pesce, Emanuela; Salis, Annalisa; Tomati, Valeria; Pastorino, Cristina; Tasso, Bruno; Benvenuti, Mirko; Damonte, Gianluca; Pedemonte, Nicoletta; Cichero, Elena; Millo, EnricoParodi, Alice; Righetti, Giada; Pesce, Emanuela; Salis, Annalisa; Tomati, Valeria; Pastorino, Cristina; Tasso, Bruno; Benvenuti, Mirko; Damonte, Gianluca; Pedemonte, Nicoletta; Cichero, Elena; Millo, Enric

G-protein coupling and nuclear translocation of the human abscisic acid receptor LANCL2

Abscisic acid (ABA), a long known phytohormone, has been recently demonstrated to be present also in humans, where it targets cells of the innate immune response, mesenchymal and hemopoietic stem cells and cells involved in the regulation of systemic glucose homeostasis. LANCL2, a peripheral membrane protein, is the mammalian ABA receptor. We show that N-terminal glycine myristoylation causes LANCL2 localization to the plasmamembrane and to cytoplasmic membrane vesicles, where it interacts with the \u3b1 subunit of a Gi protein and starts the ABA signaling pathway via activation of adenylate cyclase. Demyristoylation of LANCL2 by chemical or genetic means triggers its nuclear translocation. Nuclear enrichment of native LANCL2 is also induced by ABA treatment. Therefore human LANCL2 is a non-transmembrane G protein-coupled receptor susceptible to hormone-induced nuclear translocation