Synergistic Effect of Garcinol and Curcumin on Antiproliferative and Apoptotic Activity in Pancreatic Cancer Cells

Pancreatic cancer (PaCa) is a major health concern due to its aggressiveness and early metastasis. Current treatments for PaCa are limited by development of resistance against therapy. As an alternative strategy, we assessed the combinatorial effect of dietary compounds, garcinol and curcumin, on human PaCa cells (BxPC-3 and Panc-1). A significant (P < 0.05) dose-dependent reduction in cell viability and increase in apoptosis were observed in both cell lines as compared to untreated controls. A combination index (CI) value < 1, for a two-way comparison of curcumin and garcinol, suggests synergism. The potency (Dm) of the combination of garcinol and curcumin was 2 to 10 fold that of the individual agents. This indicates that curcumin and garcinol in combination exhibit a high level of synergism, with enhanced bioactivity, thereby reducing the required effective dose required for each individually. This combinatorial strategy may hold promise in future development of therapies against PaCa

Hydrolytic Degradation of Polylactic Acid Fibers as a Function of pH and Exposure Time

Polylactic acid (PLA) is a widely used bioresorbable polymer in medical devices owing to its biocompatibility, bioresorbability, and biodegradability. It is also considered a sustainable solution for a wide variety of other applications, including packaging. Because of its widespread use, there have been many studies evaluating this polymer. However, gaps still exist in our understanding of the hydrolytic degradation in extreme pH environments and its impact on physical and mechanical properties, especially in fibrous materials. The goal of this work is to explore the hydrolytic degradation of PLA fibers as a function of a wide range of pH values and exposure times. To complement the experimental measurements, molecular-level details were obtained using both molecular dynamics (MD) simulations with ReaxFF and density functional theory (DFT) calculations. The hydrolytic degradation of PLA fibers from both experiments and simulations was observed to have a faster rate of degradation in alkaline conditions, with 40% of strength loss of the fibers in just 25 days together with an increase in the percent crystallinity of the degraded samples. Additionally, surface erosion was observed in these PLA fibers, especially in extreme alkaline environments, in contrast to bulk erosion observed in molded PLA grafts and other materials, which is attributed to the increased crystallinity induced during the fiber spinning process. These results indicate that spun PLA fibers function in a predictable manner as a bioresorbable medical device when totally degraded at end-of-life in more alkaline conditions

Studies on plasma treatment and priming of seeds of bell pepper (Capsicum annuum L.)

The present investigation was carried out at Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan during the year 2014 -2015 to study the “Effect of cold plasma treatment and priming on bell pepper (Capsicum annuum L. cultivar California Wonder) for seed germination and seedling vigour. The seeds were ex-posed to various durations of oxygen plasma treatment using glow discharge technique at FCIPT, Institute for Plas-ma Research, Gandhinagar, Gujarat, India. Seeds were pre-treated with power of 100 W for treatment durations of 0, 3, 6, 9, 12, 15 minutes for 0, 4, 8 and 12 month durations. The changes in surface morphology of plasma treated seeds were studied by Scanning Electron Microscopy (SEM) and Contact Angle Goniometer. Along with plasma treatment, seeds were also treated with standard priming method i.e osmoprimng for comparison. Results showed that plasma treatment alone as well as in combination with osmoprimng up to 6 minutes duration had positive effects on seed germination and seedling vigour. Germination and vigour indices significantly increased by 21.75% and 90.71% respectively. Characteristics of germination percentage, speed of germination, seedling vigour index-I &amp; II, significantly increased by 13.92%, 1.39 cm, 0.38 mg, 322.07 respectively, compared with control. And it was found that immediately after plasma exposure the germination (84.41%) and vigour (228.50) was highest and it was reduced to (73.54%) and (174.27) after 12 months of storage. These results indicated that cold plasma treatment might promote the growth and modify the speed of germination i.e. higher speed of germination was observed in seeds exposed to plasma treat-ment for 6 minutes (59.82%), whereas, lowest germination speed (40.76%) was observed in untreated control

The Immirzi Parameter as an Instanton Angle

The Barbero-Immirzi parameter is a one parameter quantization ambiguity underpinning the loop approach to quantum gravity that bears tantalizing similarities to the theta parameter of gauge theories such as Yang-Mills and QCD. Despite the apparent semblance, the Barbero-Immirzi field has resisted a direct topological interpretation along the same lines as the theta-parameter. Here we offer such an interpretation. Our approach begins from the perspective of Einstein-Cartan gravity as the symmetry broken phase of a de Sitter gauge theory. From this angle, just as in ordinary gauge theories, a theta-term emerges from the requirement that the vacuum is stable against quantum mechanical tunneling. The Immirzi parameter is then identified as a combination of Newton's constant, the cosmological constant, and the theta-parameter.Comment: 24 page

Is it safe to vaccinate children against varicella while they're in close contact with a pregnant woman?

All healthy children without evidence of immunity to varicella who are living in a household with a susceptible pregnant woman should be vaccinated (strength of recommendation [SOR]: C, expert opinion). The risk of transmission of vaccine virus to household contacts is very low (SOR: B, observational studies). Transmission is higher, but still rare, among contacts of immunocompromised vaccinees (SOR: B, observational studies). Varicella infection has not been reported in unborn babies of women who had contact with a recently vaccinated person