Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle

The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Multani, Anmol. University of Calgary; CanadáFil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marin, Maia Solange. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Riviere, Nahuel Agustín. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Romero Victorica, Matias. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ma, Tao. University Of Alberta. Faculty Of Agricultural, Life And Environmental Sciences. Departament Of Agricultural, Food And Nutritional Science.; CanadáFil: Le Guan, Luo. University Of Alberta. Faculty Of Agricultural, Life And Environmental Sciences. Departament Of Agricultural, Food And Nutritional Science.; CanadáFil: Talia, Paola Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cobo, Eduardo R.. University of Calgary; Canad

Study of the B−→Λc+Λˉc−K−B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-} decay

The decay $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ is studied in proton-proton collisions at a center-of-mass energy of $\sqrt{s}=13$ TeV using data corresponding to an integrated luminosity of 5 $\mathrm{fb}^{-1}$ collected by the LHCb experiment. In the $\Lambda_{c}^+ K^{-}$ system, the $\Xi_{c}(2930)^{0}$ state observed at the BaBar and Belle experiments is resolved into two narrower states, $\Xi_{c}(2923)^{0}$ and $\Xi_{c}(2939)^{0}$, whose masses and widths are measured to be $$m(\Xi_{c}(2923)^{0}) = 2924.5 \pm 0.4 \pm 1.1 \,\mathrm{MeV}, \\ m(\Xi_{c}(2939)^{0}) = 2938.5 \pm 0.9 \pm 2.3 \,\mathrm{MeV}, \\ \Gamma(\Xi_{c}(2923)^{0}) = \phantom{000}4.8 \pm 0.9 \pm 1.5 \,\mathrm{MeV},\\ \Gamma(\Xi_{c}(2939)^{0}) = \phantom{00}11.0 \pm 1.9 \pm 7.5 \,\mathrm{MeV},$$ where the first uncertainties are statistical and the second systematic. The results are consistent with a previous LHCb measurement using a prompt $\Lambda_{c}^{+} K^{-}$ sample. Evidence of a new $\Xi_{c}(2880)^{0}$ state is found with a local significance of $3.8\,\sigma$, whose mass and width are measured to be $2881.8 \pm 3.1 \pm 8.5\,\mathrm{MeV}$ and $12.4 \pm 5.3 \pm 5.8 \,\mathrm{MeV}$, respectively. In addition, evidence of a new decay mode $\Xi_{c}(2790)^{0} \to \Lambda_{c}^{+} K^{-}$ is found with a significance of $3.7\,\sigma$. The relative branching fraction of $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ with respect to the $B^{-} \to D^{+} D^{-} K^{-}$ decay is measured to be $2.36 \pm 0.11 \pm 0.22 \pm 0.25$, where the first uncertainty is statistical, the second systematic and the third originates from the branching fractions of charm hadron decays.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-028.html (LHCb public pages

Measurement of the ratios of branching fractions R(D∗)\mathcal{R}(D^{*}) and R(D0)\mathcal{R}(D^{0})

The ratios of branching fractions $\mathcal{R}(D^{*})\equiv\mathcal{B}(\bar{B}\to D^{*}\tau^{-}\bar{\nu}_{\tau})/\mathcal{B}(\bar{B}\to D^{*}\mu^{-}\bar{\nu}_{\mu})$ and $\mathcal{R}(D^{0})\equiv\mathcal{B}(B^{-}\to D^{0}\tau^{-}\bar{\nu}_{\tau})/\mathcal{B}(B^{-}\to D^{0}\mu^{-}\bar{\nu}_{\mu})$ are measured, assuming isospin symmetry, using a sample of proton-proton collision data corresponding to 3.0 fb${ }^{-1}$ of integrated luminosity recorded by the LHCb experiment during 2011 and 2012. The tau lepton is identified in the decay mode $\tau^{-}\to\mu^{-}\nu_{\tau}\bar{\nu}_{\mu}$. The measured values are $\mathcal{R}(D^{*})=0.281\pm0.018\pm0.024$ and $\mathcal{R}(D^{0})=0.441\pm0.060\pm0.066$, where the first uncertainty is statistical and the second is systematic. The correlation between these measurements is $\rho=-0.43$. Results are consistent with the current average of these quantities and are at a combined 1.9 standard deviations from the predictions based on lepton flavor universality in the Standard Model.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-039.html (LHCb public pages

Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis

A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle.Instituto de BiotecnologíaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Bianco, Marí­a Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Gravisaco, Marí­a José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Alfonseca, Edgar. Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; MéxicoFil: Singh, Mahavir G. Lionnex; AlemaniaFil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; ArgentinaFil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Buddle, Bryce Malcom. AgResearch. Hopkirk Research Institute; Nueva ZelandaFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentin

Study of b−b-hadron decays to Λc+h−h′−\Lambda_c^+h^-h^{\prime -} final states

Decays of $\Xi_{b}^{-}$ and $\Omega_{b}^{-}$ baryons to $\Lambda_{c}^{+} h^- h^{\prime -}$ final states, with $h^- h^{\prime -}$ being $\pi^{-}\pi^{-}$, $K^{-}\pi^{-}$ and $K^{-}K^{-}$ meson pairs, are searched for using data collected with the LHCb detector. The data sample studied corresponds to an integrated luminosity of $8.7 \rm fb^{-1}$ of $pp$ collisions collected at centre-of-mass energies $\sqrt{s} = 7$, $8$ and $13 \rm TeV$. The products of the relative branching fractions and fragmentation fractions for each signal mode, relative to the $B^{-} \rightarrow \Lambda_{c}^{+} \bar{p} \pi^{-}$ mode, are measured, with $\Xi_{b}^{-} \rightarrow\Lambda_{c}^{+} K^{-} \pi^{-}$, $\Xi_{b}^{-} \rightarrow\Lambda_{c}^{+} K^{-} K^{-}$ and $\Omega_{b}^{-} \rightarrow\Lambda_{c}^{+} K^{-} K^{-}$ decays being observed at over $5\,\sigma$ significance. The $\Xi_{b}^{-} \rightarrow\Lambda_{c}^{+} K^{-} \pi^{-}$ mode is also used to measure the $\Xi_{b}^{-}$ production asymmetry, which is found to be consistent with zero. In addition, the $B^{-} \rightarrow \Lambda_{c}^{+} \bar{p} K^{-}$ decay is observed for the first time, and its branching fraction is measured relative to that of the $B^{-} \rightarrow \Lambda_{c}^{+} \bar{p} \pi^{-}$ mode.Decays of $\Xi_b^-$ and $\Omega_b^-$ baryons to $\Lambda_c^+ h^- h^{\prime -}$ final states, with $h^- h^{\prime -}$ being $\pi^-\pi^-$, $K^-\pi^-$ and $K^-K^-$ meson pairs, are searched for using data collected with the LHCb detector. The data sample studied corresponds to an integrated luminosity of $8.7\,\mathrm{fb}^{-1}$ of $pp$ collisions collected at centre-of-mass energies $\sqrt{s} = 7$, $8$ and $13\,\mathrm{Te\kern -0.1em V}$. The products of the relative branching fractions and fragmentation fractions for each signal mode, relative to the $B^- \to \Lambda_c^+ \overline{p} \pi^-$ mode, are measured, with $\Xi_{b}^- \to\Lambda_{c}^+ K^- \pi^-$, $\Xi_{b}^- \to\Lambda_{c}^+ K^- K^-$ and $\Omega_{b}^- \to\Lambda_{c}^+ K^- K^-$ decays being observed at over $5\,\sigma$ significance. The $\Xi_{b}^- \to\Lambda_{c}^+ K^- \pi^-$ mode is also used to measure the $\Xi_{b}^-$ production asymmetry, which is found to be consistent with zero. In addition, the $B^- \to \Lambda_{c}^+ \overline{p} K^-$ decay is observed for the first time, and its branching fraction is measured relative to that of the $B^- \to \Lambda_{c}^+ \overline{p} \pi^-$ mode

Search for the Bs0→μ+μ−γB_s^0 \rightarrow \mu^+\mu^-\gamma decay

International audienceA search for the fully reconstructed $B_s^0 \rightarrow \mu^+\mu^-\gamma$ decay is performed at the LHCb experiment using proton-proton collisions at $\sqrt{s}=13$ TeV corresponding to an integrated luminosity of $5.4\,\mathrm{fb^{-1}}$. No significant signal is found and upper limits on the branching fraction in intervals of the dimuon mass are set \begin{align} {\cal B}(B_s^0 \rightarrow \mu^+\mu^-\gamma) < 4.2\times10^{-8},~&m(\mu\mu)\in[2m_\mu,~1.70]\,\mathrm{GeV/c^2} ,\nonumber {\cal B}(B_s^0 \rightarrow \mu^+\mu^-\gamma) < 7.7\times10^{-8},~&m(\mu\mu)\in[1.70,~2.88]\,\mathrm{GeV/c^2},\nonumber {\cal B}(B_s^0 \rightarrow \mu^+\mu^-\gamma) < 4.2\times10^{-8},~&m(\mu\mu)\in[3.92 ,~m_{B_s^0}]\,\mathrm{GeV/c^2},\nonumber \end{align} at 95% confidence level. Additionally, upper limits are set on the branching fraction in the $[2m_\mu,~1.70]\,\mathrm{GeV/c^2}$ dimuon mass region excluding the contribution from the intermediate $\phi(1020)$ meson, and in the region combining all dimuon-mass intervals

Transverse polarisation measurement of Λ\Lambda hyperons in ppNe collisions at sNN\sqrt{s_{NN}}=68.4 GeV with the LHCb detector

A measurement of the transverse polarization of the $\Lambda$ and $\bar{\Lambda}$hyperons in $p$Ne fixed-target collisions at $\sqrt{s_{NN}}$=68.4 GeV is presented using data collected by the LHCb detector. The polarization is studied using the decay $\Lambda \rightarrow p \pi^-$ together with its charge conjugated process, the integrated values measured are $$P_{\Lambda} = 0.029 \pm 0.019 \, (\rm{stat}) \pm 0.012 \, (\rm{syst}) \, ,$$ $$P_{\bar{\Lambda}} = 0.003 \pm 0.023 \, (\rm{stat}) \pm 0.014 \,(\rm{syst}) \,$$ Furthermore, the results are shown as a function of the Feynman $x$ variable, transverse momentum, pseudorapidity and rapidity of the hyperons, and are compared with previous measurements.A measurement of the transverse polarization of the $\Lambda$ and $\bar{\Lambda}$ hyperons in $p$Ne fixed-target collisions at $\sqrt{s_{NN}}$ = 68.4 GeV is presented using data collected by the LHCb detector. The polarization is studied using the decay $\Lambda \rightarrow p \pi^-$ together with its charge conjugated process, the integrated values measured are $$P_{\Lambda} = 0.029 \pm 0.019 \, (\rm{stat}) \pm 0.012 \, (\rm{syst}) \, ,$$ $$P_{\bar{\Lambda}} = 0.003 \pm 0.023 \, (\rm{stat}) \pm 0.014 \,(\rm{syst}) \,.$$ Furthermore, the results are shown as a function of the Feynman~$x$~variable, transverse momentum, pseudorapidity and rapidity of the hyperons, and are compared with previous measurements

Tracking of charged particles with nanosecond lifetimes at LHCb

A method is presented to reconstruct charged particles with lifetimes between 10 ps and 10 ns, which considers a combination of their decay products and the partial tracks created by the initial charged particle. Using the $\Xi^-$ baryon as a benchmark, the method is demonstrated with simulated events and proton-proton collision data at $\sqrt{s}=13$ TeV, corresponding to an integrated luminosity of 2.0 fb${}^{-1}$ collected with the LHCb detector in 2018. Significant improvements in the angular resolution and the signal purity are obtained. The method is implemented as part of the LHCb Run 3 event trigger in a set of requirements to select detached hyperons. This is the first demonstration of the applicability of this approach at the LHC, and the first to show its scaling with instantaneous luminosity

Search for the Bs0→μ+μ−γB_s^0 \rightarrow \mu^+\mu^-\gamma decay

International audienceA search for the fully reconstructed $B_s^0 \rightarrow \mu^+\mu^-\gamma$ decay is performed at the LHCb experiment using proton-proton collisions at $\sqrt{s}=13$ TeV corresponding to an integrated luminosity of $5.4\,\mathrm{fb^{-1}}$. No significant signal is found and upper limits on the branching fraction in intervals of the dimuon mass are set \begin{align} {\cal B}(B_s^0 \rightarrow \mu^+\mu^-\gamma) < 4.2\times10^{-8},~&m(\mu\mu)\in[2m_\mu,~1.70]\,\mathrm{GeV/c^2} ,\nonumber {\cal B}(B_s^0 \rightarrow \mu^+\mu^-\gamma) < 7.7\times10^{-8},~&m(\mu\mu)\in[1.70,~2.88]\,\mathrm{GeV/c^2},\nonumber {\cal B}(B_s^0 \rightarrow \mu^+\mu^-\gamma) < 4.2\times10^{-8},~&m(\mu\mu)\in[3.92 ,~m_{B_s^0}]\,\mathrm{GeV/c^2},\nonumber \end{align} at 95% confidence level. Additionally, upper limits are set on the branching fraction in the $[2m_\mu,~1.70]\,\mathrm{GeV/c^2}$ dimuon mass region excluding the contribution from the intermediate $\phi(1020)$ meson, and in the region combining all dimuon-mass intervals

First observation of Λb0→Σc(∗)++D(∗)−K−\Lambda_{b}^{0} \rightarrow \Sigma_c^{(*)++} D^{(*)-} K^{-} decays

International audienceThe four decays, $\Lambda_{b}^{0} \rightarrow \Sigma_c^{(*)++} D^{(*)-} K^{-}$, are observed for the first time using proton-proton collision data collected with the LHCb detector at a centre-of-mass energy of $13\,\rm{TeV}$, corresponding to an integrated luminosity of $6\,\rm{fb}^{-1}$. By considering the $\Lambda_b^0 \rightarrow \Lambda_c^{+} \overline{D}^0 K^{-}$ decay as reference channel, the following branching fraction ratios are measured to be, $$\frac{\cal{B} (\Lambda_{b}^{0} \rightarrow \Sigma_{c}^{++} \rm{D}^{-} {K}^{-})}{\cal{B}(\Lambda_{b}^{0} \rightarrow \Lambda_c^{+} \rm \overline{D}^0 {K}^{-})} = {0.282}\pm{0.016}\pm{0.016}\pm{0.005}, \frac{\cal{B}(\Lambda_{b}^{0} \rightarrow \Sigma_{c}^{*++} \rm {D}^{-} {K}^{-})}{\cal{B}(\Lambda_{b}^{0} \rightarrow \Sigma_c^{++} \rm {D}^{-} {K}^{-})} = {0.460}\pm{0.052}\pm{0.028}, \frac{\cal{B}(\Lambda_{b}^{0} \rightarrow \Sigma_{c}^{++} \rm {D}^{*-} {K}^{-})}{\cal{B}(\Lambda_{b}^{0} \rightarrow \Sigma_c^{++} \rm {D}^{-} {K}^{-})} = {2.261}\pm{0.202}\pm{0.129}\pm{0.046}, \frac{\cal{B}(\Lambda_{b}^{0} \rightarrow \Sigma_{c}^{*++} \rm D^{*-} K^{-})}{\cal{B}(\Lambda_{b}^{0} \rightarrow \Sigma_c^{++} \rm D^{-} K^{-})} = {0.896}\pm{0.137}\pm{0.066}\pm{0.018},$$ where the first uncertainties are statistical, the second are systematic, and the third are due to uncertainties in the branching fractions of intermediate particle decays. These initial observations mark the beginning of pentaquark searches in these modes, with more data set to become available following the LHCb upgrade