Análisis de niveles de resiliencia en escolares antioqueños expuestos a problemáticas de violencias: propuesta pedagógica para la implementación de la cátedra de la paz

Este trabajo tiene como investigar, evaluar los niveles de resiliencia en los escolares de una de las instituciones públicas del municipio, una institución de inclusión donde se recibe a los estudiantes expulsados de las demás instituciones municipales, realidades atravesadas por el dolor, la guerra, la pobreza y la esperanza agónica. Analizar los niveles de resiliencia de los estudiantes grado de décimo y once y su relación con el contexto de violencia al que están expuestos los adolescentes, permitir a través de este análisis, presentar a la comunidad académica y social una estrategia pedagógica para ser desarrollada a través de la cátedra de la paz, que favorezca la convivencia y la construcción de paz desde el colegio.Magister en EducaciónMaestrí

Allele- and cell-type-specific effects of human IL10 on intestinal health and disease

There is growing recognition that non-protein-coding DNA may have crucial gene regulatory functions. However, the role of human non-coding DNA in disease pathogenesis is unclear and notoriously difficult to study. Here, we present two novel mouse models expressing distinct donor-derived human IL10 genes of the “ATA” and “GCC” haplotypes, named based on the “classic” biallelic promoter single nucleotide polymorphisms (SNPs) that they differentially express. We use a well-established mouse model of dextran sulfate sodium (DSS)-induced colitis to trigger intestinal inflammation and bacteremia in these mice, and found that there are clear haplotype-dependent differences in mortality and morbidity rates, with the ATA mice being highly resistant to DSS-induced clinical symptoms and death compared to both the GCCs or mouse IL-10- expressing wild-types (WT). Further characterization through flow cytometry and intestinal explants reveal that the ATAs express low levels of human IL-10 and high IL-17 production throughout the gastrointestinal tract, and have lower bacterial loads in their blood and filtering organs as compared to GCCs and WTs, suggesting that the human IL10 ATA allele may confer protection against colitis-induced gut bacterial dissemination and consequently sepsis. Our findings highlight the importance of human non-coding DNA in disease susceptibility and outcome; they also serve as a proof-of-concept for the use of genetically modified “humanized” mice in the functional and phenotypic characterization of human non-coding DNA

Allele- and cell-type-specific effects of human IL10 on intestinal health and disease

There is growing recognition that non-protein-coding DNA may have crucial gene regulatory functions. However, the role of human non-coding DNA in disease pathogenesis is unclear and notoriously difficult to study. Here, we present two novel mouse models expressing distinct donor-derived human IL10 genes of the “ATA” and “GCC” haplotypes, named based on the “classic” biallelic promoter single nucleotide polymorphisms (SNPs) that they differentially express. We use a well-established mouse model of dextran sulfate sodium (DSS)-induced colitis to trigger intestinal inflammation and bacteremia in these mice, and found that there are clear haplotype-dependent differences in mortality and morbidity rates, with the ATA mice being highly resistant to DSS-induced clinical symptoms and death compared to both the GCCs or mouse IL-10- expressing wild-types (WT). Further characterization through flow cytometry and intestinal explants reveal that the ATAs express low levels of human IL-10 and high IL-17 production throughout the gastrointestinal tract, and have lower bacterial loads in their blood and filtering organs as compared to GCCs and WTs, suggesting that the human IL10 ATA allele may confer protection against colitis-induced gut bacterial dissemination and consequently sepsis. Our findings highlight the importance of human non-coding DNA in disease susceptibility and outcome; they also serve as a proof-of-concept for the use of genetically modified “humanized” mice in the functional and phenotypic characterization of human non-coding DNA

Sex-specific effects of Sca-1/Ly-6A on development of B lymphocyte in the bone marrow (P4384)

Abstract Stem cell antigen-1 (Sca-1, also known as Ly-6A) is highly expressed on the hematopoietic stem cells (HSC) and on early lymphoid progenitors, but the role this cell surface protein plays in the development of B cells in the bone marrow is unknown. We report here that the absence of Sca-1/ Ly-6A protein in mice regulates B cell development in the bone marrow in a sex-specific manner. Significant impairment of B cell development was observed in female mice lacking the Sca-1/Ly-6A protein, while this impairment was not observed in Sca-1/Ly-6A deficient males. In contrast, higher percentages of NK1.1+ cells were observed in the thymus from Sca-1/Ly-6A deficient females, while the male mice lacking Sca-1/Ly-6A did not show any alteration when compared to the wild type controls. The deficiency of Sca-1/Ly-6A did not impair development of T cells in the thymus of mice of either sex. These findings indicate that Sca-1/Ly-6A plays an important role in B cell and NK1.1+ cell development and suggests a functional interaction between Sca-1/Ly-6A and female sex hormones in regulating development of B cells and NK1.1+ cells.</jats:p

Resolution of reactive angioendotheliomatosis in an arteriovenous fistula with innominate vein angioplasty

Introduction: Arteriovenous fistulae (AVF)-associated reactive angioendotheliomatosis (RAE) is a very rare entity (three previously reported cases in the literature) that can manifest as extremity wounds. RAE’s etiopathology is unknown. Case description: We report a case of severe limb-threatening upper extremity wound with pathology-proven RAE. This lesion was previously refractory to standard wound care. There was no evidence of limb ischemia or steal syndrome, previously deemed to be the underlying cause of AVF-associated RAE in other reports. Conclusions: Successful endovascular treatment of an ipsilateral innominate vein stenosis led to reduction of venous hypertension, resolution of associated arm edema, and subsequent wound healing. We therefore propose that venous engorgement and hypertension from central venous stenosis is the likely underlying cause for AVF-associated RAE. If this rare entity is encountered in the setting of AVF, there is utility in treating the wound as a sentinel lesion and venography should be conducted to rule out central venous pathology. Vascular intervention complements aggressive local wound management and biopsy is requisite for prompt diagnosis. </jats:sec

Viability and functionality of cells delivered from peptide conjugated scaffolds

Many cell-based therapies aim to transplant functional cells to revascularize damaged tissues and ischemic areas. However, conventional cell therapy is not optimally efficient: massive cell death, damage, and non-localization of cells both spatially and temporally all likely contribute to poor tissue functionality. An alginate cell depot system has been proposed as an alternative means to deliver outgrowth endothelial cells (OECs) in a spatiotemporally controllable manner while protecting them in the early stages of tissue re-integration. Here OECs exiting the alginate scaffold were measured for viability, functionality, and migration speed and characterized for cytokine and surface marker profiles. OECs were highly viable in the alginate and were depleted from the scaffold via migration at a speed of 21±6µm/hr following release. Prolonged interaction with the alginate scaffold microenvironment did not detrimentally change OECs; they retained high functionality, displayed a similar angiogenic cytokine profile as control OECs, and did not have significantly altered surface markers. These results suggest that alginate-OEC interactions do not adversely affect these cells, validating control of cellular migration as a means to control the cell delivery profile from the material system, and supporting usage of the alginate scaffold as an efficient cell delivery vehicle

Is sentinel lymph node biopsy without frozen section in early stage breast cancer sufficient in accordance with ACOSOG-Z0011? A retrospective review from King Chulalongkorn Memorial Hospital.

Abstract Background: In 2021, increased implementation of the usage of permanent section (PS) without intraoperative frozen section (FS) for sentinel lymph node biopsy (SLNB) were evident worldwide. This pilot study conducted in Thailand will determine the re-operation rate in SLNB without FS technique. Method: we retrospectively reviewed 239 cases of SLNB without FS in King Chulalongkorn Memorial Hospital from April 2016 to April 2021. These patients were diagnosed with primary invasive breast cancer with clinical node negative. The clinical nodal status was assessed from physical examination. The re-operation rate was evaluated by the number of positive SLNs; equal or more than 3 nodal metastases were subjected to second surgical procedure. Result: Between April 2016 and April 2021, there are 239 patient who had undergone SLNB in accordance with ACOSOG Z0011 criteria with the use of PS alone. A total of 975 SLNs were removed from these 239 patients, with an average of 4.15 nodes per patient. Out of 239 patients, 21 (8.8%) and 6 (2.5%) had metastatic disease in 1 and 2 nodes, respectively. The remaining 212 (88.7%) patients had no nodal metastasis. None of the patients were subjected to a second surgical procedure.Conclusion: With a re-operation rate of 0%, we concluded that the implementation of SLNB with PS analysis alone in patients who satisfied the ACOSOG Z0011 criteria do not altered the standard of care offered by additional FS analysis. With cessation of intraoperative procedure, operation cost, operation time and anesthetic side effect are projected to decrease.</jats:p