12 research outputs found
Loop-Mediated Isothermal Amplification (LAMP)-Based Method for Rapid Mushroom Species Identification
Toxic mushroom species, such as the death cap (Amanita
phalloides), are responsible for most mushroom poisonings.
In the present work, novel loop-mediated isothermal amplification
(LAMP) assays were used for the differentiation of even closely related
edible and toxic mushroom species. The applicability of these methods
was tested by cross-reaction studies and analysis of spiked mushroom
samples (raw and fried material). Contaminations at the level of 2%
(w/w) could be detected in different mushroom blends. Three detection
methods were used: agarose gel analysis, fluorimetric real-time detection,
and visual detection by lateral flow dipsticks (LFD). The LAMP assay
combined with LFD detection allows the identification of A. phalloides in about 2 h (including DNA extraction)
at a very low level of technical equipment (micropestle, water bath,
and mobile centrifuge), which makes this technique perfectly suited
for on-site applications