Proton Motive Force-Dependent Hoechst 33342 Transport by the ABC Transporter LmrA of Lactococcus lactis

The fluorescent compound Hoechst 33342 is a substrate for many multidrug resistance (MDR) transporters and is widely used to characterize their transport activity. We have constructed mutants of the adenosine triphosphate (ATP) binding cassette (ABC)-type MDR transporter LmrA of Lactococcus lactis that are defective in ATP hydrolysis. These mutants and wild-type LmrA exhibited an atypical behavior in the Hoechst 33342 transport assay. In membrane vesicles, Hoechst 33342 transport was shown to be independent of the ATPase activity of LmrA, and it was not inhibited by orthovanadate but sensitive to uncouplers that collapse the proton gradient and to N,N'-dicyclohexylcarbodiimide, an inhibitor of the F0F1-ATPase. In contrast, transport of Hoechst 33342 by the homologous, heterodimeric MDR transporter LmrCD showed a normal ATP dependence and was insensitive to uncouplers of the proton gradient. With intact cells, expression of LmrA resulted in an increased rate of Hoechst 33342 influx while LmrCD caused a decrease in the rate of Hoechst 33342 influx. Cellular toxicity assays using a triple knockout strain, i.e., L. lactis ΔlmrA ΔlmrCD, demonstrate that expression of LmrCD protects cells against the growth inhibitory effects of Hoechst 33342, while in the presence of LmrA, cells are more susceptible to Hoechst 33342. Our data demonstrate that the LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane.

Superheating and solid-liquid phase coexistence in nanoparticles with non-melting surfaces

We present a phenomenological model of melting in nanoparticles with facets that are only partially wet by their liquid phase. We show that in this model, as the solid nanoparticle seeks to avoid coexistence with the liquid, the microcanonical melting temperature can exceed the bulk melting point, and that the onset of coexistence is a first-order transition. We show that these results are consistent with molecular dynamics simulations of aluminum nanoparticles which remain solid above the bulk melting temperature.Comment: 8 pages, 5 figure

Long Period Variable Stars: galactic populations and infrared luminosity calibrations

In this paper HIPPARCOS astrometric and kinematic data are used to calibrate both infrared luminosities and kinematical parameters of Long Period Variable stars (LPVs). Individual absolute K and IRAS 12 and 25 luminosities of 800 LPVs are determined and made available in electronic form. The estimated mean kinematics is analyzed in terms of galactic populations. LPVs are found to belong to galactic populations ranging from the thin disk to the extended disk. An age range and a lower limit of the initial mass is given for stars of each population. A difference of 1.3mag in K for the upper limit of the Asymptotic Giant Branch is found between the disk and old disk galactic populations, confirming its dependence on the mass in the main sequence. LPVs with a thin envelope are distinguished using the estimated mean IRAS luminosities. The level of attraction (in the classification sense) of each group for the usual classifying parameters of LPVs (variability and spectral types) is examined.Comment: Accepted for publication in A&A (May 2001). 13 page

A spectroscopic atlas of post-AGB stars and planetary nebulae selected from the IRAS Point Source Catalogue

Aims: We study the optical spectral properties of a sample of stars showing far infrared colours similar to those of well-known planetary nebulae. The large majority of them were unidentified sources or poorly known in the literature at the time when this spectroscopic survey started, some 15 years ago. Methods: We present low-resolution optical spectroscopy, finding charts and improved astrometric coordinates of a sample of 253 IRAS sources. Results: We have identified 103 sources as post-AGB stars, 21 as ``transition sources'', and 36 as planetary nebulae, some of them strongly reddened. Among the rest of sources in the sample, we were also able to identify 38 young stellar objects, 5 peculiar stars, and 2 Seyfert galaxies. Up to 49 sources in our spectroscopic sample do not show any optical counterpart, and most of them are suggested to be heavily obscured post-AGB stars, rapidly evolving on their way to becoming planetary nebulae. Conclusions: An analysis of the galactic distribution of the sources identified as evolved stars in the sample is presented together with a study of the distribution of these stars in the IRAS two-colour diagram. Finally, the spectral type distribution and other properties of the sources identified as post-AGB in this spectroscopic survey are discussed in the framework of stellar evolution.Comment: 69 pages, 413 figures. Accepted by Astronomy and Astrophysic

MSX versus IRAS Two-Color Diagrams and the CSE-Sequence of Oxygen-Rich Late-Type Stars

We present MSX two-color diagrams that can be used to characterize circumstellar environments of sources with good quality MSX colors in terms of IRAS color regions for oxygen-rich stars. With these diagrams we aim to provide a new tool that can be used to study circumstellar environments and to improve detection rates for targeted surveys for circumstellar maser emission similar to the IRAS two-color diagram. This new tool is especially useful for regions in the sky where IRAS was confused, in particular in the Galactic plane and bulge region. Unfortunately, using MSX colors alone does not allow to distinguish between carbon-rich and oxygen-rich objects. An application of this tool on 86 GHz SiO masers shows that for this type of masers an instantaneous detection rate of 60% to 80% can be achieved if target sources are selected according to MSX color (region). Our investigations may have revealed an error in the MSX point source catalog version 2.3. That is, the photometry of the 21.3 $\mu$m (MSX E filter) band for most weak 8.28 $\mu$m (or MSX A filter) band sources seems off by about a factor two (0.5--1 magnitude too bright).Comment: accepted to Astrophysical Journal, 11 pages, 11 figs of which 1 in colo

Water maser detections in southern candidates to post-AGB stars and Planetary Nebulae

We intended to study the incidence and characteristics of water masers in the envelopes of stars in the post-AGB and PN evolutionary stages. We have used the 64-m antenna in Parkes (Australia) to search for water maser emission at 22 GHz, towards a sample of 74 sources with IRAS colours characteristic of post-AGB stars and PNe, at declination $< -32 \deg$. In our sample, 39% of the sources are PNe or PNe candidates, and 50% are post-AGB stars or post-AGB candidates. We have detected four new water masers, all of them in optically obscured sources: three in PNe candidates (IRAS 12405-6219, IRAS 15103-5754, and IRAS 16333-4807); and one in a post-AGB candidate (IRAS 13500-6106). The PN candidate IRAS 15103-5754 has water fountain characteristics, and it could be the first PN of this class found. We confirm the tendency suggested in Paper I that the presence of water masers in the post-AGB phase is favoured in obscured sources with massive envelopes. We propose an evolutionary scenario for water masers in the post-AGB and PNe stages, in which ``water fountain'' masers could develop during post-AGB and early PN stages. Later PNe would show lower velocity maser emission, both along jets and close to the central objects, with only the central masers remaining in more evolved PNe.Comment: 11 pages, 5 figures. Accepted by Astronomy and Astrophysic

Long-term transients help explain regime shifts in consumer-renewable resource systems

As planetary boundaries loom, there is an urgent need to develop sustainable equilibriums between societies and the resources they consume, thereby avoiding regime shifts to undesired states. Transient system trajectories to a stable state may differ substantially, posing significant challenges to distinguishing sustainable from unsustainable trajectories. We use stylized models to show how feedbacks between anthropogenic harvest regimes and resource availability drive transient dynamics. We show how substantial time lags may occur between interventions and social-ecological outcomes, and that sudden system collapses need not be linked to recent environmental changes. Historical reconstructions of island state populations show a variety of transient dynamics that closely corresponds to model expectations based on island differences in productivity and harvesting regime. We conclude that vulnerable social-ecological systems may persist when the population:resource ratio remains within a viable range of intermediate (rather than small) values, which implies that averting environmental crises may require counter-intuitive measures