Molecular evolution of candidate male reproductive genes in the brown algal model Ectocarpus

Background: Evolutionary studies of genes that mediate recognition between sperm and egg contribute to our understanding of reproductive isolation and speciation. Surface receptors involved in fertilization are targets of sexual selection, reinforcement, and other evolutionary forces including positive selection. This observation was made across different lineages of the eukaryotic tree from land plants to mammals, and is particularly evident in free-spawning animals. Here we use the brown algal model species Ectocarpus (Phaeophyceae) to investigate the evolution of candidate gamete recognition proteins in a distant major phylogenetic group of eukaryotes. Results: Male gamete specific genes were identified by comparing transcriptome data covering different stages of the Ectocarpus life cycle and screened for characteristics expected from gamete recognition receptors. Selected genes were sequenced in a representative number of strains from distant geographical locations and varying stages of reproductive isolation, to search for signatures of adaptive evolution. One of the genes (Esi0130_0068) showed evidence of selective pressure. Interestingly, that gene displayed domain similarities to the receptor for egg jelly (REJ) protein involved in sperm-egg recognition in sea urchins. Conclusions: We have identified a male gamete specific gene with similarity to known gamete recognition receptors and signatures of adaptation. Altogether, this gene could contribute to gamete interaction during reproduction as well as reproductive isolation in Ectocarpus and is therefore a good candidate for further functional evaluation

Psychiatric disorders and future violent arrests : a prospective study among detained girls

This study scrutinizes if detained girls with psychiatric disorders were at risk for future violent arrests during adolescence. A structured diagnostic interview was performed to determine the presence of various psychiatric disorders in 313 detained girls. Official juvenile arrest records were collected. With three exceptions that are in need of replication, psychiatric disorders (e.g., post-traumatic stress disorder, subtypes of conduct disorder), psychiatric disorder categories (e.g., anxiety disorders), and psychiatric comorbidity patterns were not prospectively related to future violent arrests. Our findings suggest that detained girls with psychiatric disorders should not be considered more dangerous than their counterparts without disorders, at least not during adolescence

The DSM-5 with limited prosocial emotions specifier for conduct disorder : a systematic literature review

DSM-5 added a categorically defined specifier ('with Limited Prosocial Emotions'; LPE) for the diagnosis of conduct disorder (CD). This paper systematically reviews the evidence base for this specifier in children and adolescents who are diagnosed with CD. Computer-assisted searches were executed and identified 181 potentially relevant papers. Eventually, nine papers were included in this review, referring to eight unique samples. All studies constructed an LPE measure by pulling the same items from the same rating scales that were used in the development of the DSM-5 LPE specifier. The prevalence of youth with CD who met criteria for this novel LPE specifier (CD + LPE) ranged from 6.1% to 83.7%. The studies greatly varied in the features used to test the viability of the DSM-5 LPE specifier. The most commonly used features relate to severity of antisocial behavior, low neuroticism (or lack of anxiety and depression), and treatment responsiveness. Available work altogether showed that CD + LPE youth displayed higher levels of past antisocial behaviour than CD Only youth, but failed to reveal other group differences that corroborate with expectations. Effect sizes typically were in the small to moderate range, suggesting that the practical usefulness of the group differences is limited. Empirical work shows that this specifier should not be used for clinical decision-making when relying on items from measures that have been used in the development of the LPE specifier. Crucially, limitations that hallmark the few studies on the topic hamper any firm conclusion about the usefulness of the specifier

Unmanned Aerial Systems for Rapid Mapping - UASRapidMap 2013 4th JRC ECML Crisis Management Technology Workshop

The 4th JRC ECML Crisis Management Technology Workshop on Unmanned Aerial Systems for Rapid Mapping was co-organised by the European Commission Joint Research Centre and the United Nations Institute for Training and Research - Operational Satellite Applications Programme (UNITAR - UNOSAT). It took place in Geneva & Dardagny, Switzerland from 11 to 13 September 2013. 74 participants from UN and EC stakeholders, NGOs, civil protection bodies, academia, and industry attended the workshop. The workshop's purpose was to present, demonstrate, and explore the state-of-the-art and future potential of unmanned aerial systems for rapid mapping applications in the context of humanitarian crisis aid and natural disaster relief operations. Main impressions from the workshop were the diversity in technological solutions for various practical uses, the rapid turnaround time from flight to having useable data at hand in the field and a reality check on what are still challenges related to flight permissions. Within the European Union a process on the harmonisation of the diverse regulations for UAS operations and the introduction of UAS into the civil airspace is ongoing. The UAS technology will most likely make a large impact on data collection in future emergency situations. In addition, based on what was demonstrated, the tools are also useful for disaster risk reduction activities.JRC.G.2-Global security and crisis managemen

Long-term antibody persistence after vaccination with a 2-dose Havrix™ (inactivated hepatitis A vaccine): 20 years of observed data, and long-term model-based predictions

AbstractAntibody persistence in two cohorts of adults, who received inactivated hepatitis A (HAV) vaccine (1440El.U; Havrix™; GSK Vaccines) according to a 0–6 or 0–12 month schedule in 1992–1993, has been measured annually. After 20 years, >97% of the subjects in both studies were seropositive for anti-HAV antibodies. Geometric mean concentrations in the according-to-protocol cohorts were 312mIU/ml in 34/36 subjects vaccinated initially at 0–6 months (NCT00289757) and 317mIU/ml in 85/86 subjects vaccinated at 0–12 months (NCT00291876). Over the whole follow-up period, seven subjects (2+5, respectively) lost circulating anti-HAV antibodies but mounted a strong response after HAV booster administration (1440El.U). Mathematical modelling, which was applied to assess true persistence at Year 20 (accounting for drop-outs and missing data), and to predict longer-term persistence confirmed previous estimates that seropositive anti-HAV levels would persist in ≥95% vaccinees at Year 30 and ≥90% at Year 40.ClinicalTrials.Gov number: NCT00289757/NCT0029187

A pulsed field gradient and NMR imaging investigations of the water retention mechanism by cellulose ethers in mortars

International audienceThe study presented in this paper is devoted to improve the knowledge on the influence of cellulose ethers (CE) on the freshly-mixed mortars water retention. Indeed, this crucial property is the most important imparted by these polysaccharides. One of the assumptions proposed to explain this phenomenon is that CE acts as diffusion barrier to the water. To test this hypothesis, the CE effect on the self-diffusion coefficient of water in solution and on the water mobility between two fresh cement pastes was studied by Nuclear Magnetic Resonance. CE does not significantly modify the water self-diffusion coefficient in CE solution or in admixed cement pastes. Moreover the interdiffusion imaging experiments demonstrated that the water diffusion at the paste/paste interface is not affected by the presence of cellulosic admixture

Impact of the substitution distribution and the interlayer distance on both the surface energy and the hydration energy for Pb-montmorillonite

International audienceThe understanding of the adsorption of charged species in swelling clays is of primary importance to optimise the capture of heavy metal ions in soils. Adsorption is expected to depend on the microscopic structure of the clay, which varies strongly with the origin and history (hydration-desorption cycles, temperature ...) of the sample. Then it is necessary to evaluate the distinct interaction energies between water molecules, interlayer cations and clay surfaces, taking into account the position of the substitutions in the clay layer. Here we propose an investigation of plausible structures of some Pb-montmorillonites differing by their interlayer distances, their layer charges and the distribution of substitutions in octahedral sheet from a modelling approach, looking at the evolution of the partial charges in these structures. Using a methodology previously developed in the simpler case of alkali cations, we also estimate the surface energy in the dry state and then the details of the hydration energy for the cation and for the clay layer to interpret the capacity of ion adsorption in swelling clays to capture heavy metals. We conclude from our calculations that a cell structure with intermediate layer charge (close to 0.5 per unit cell) is probably the best candidate to allow the capture of the Pb2+ as interlayer cations considering the electrostatic effect. The hydration process of the cation appears as the most energetic part of the hydration of swelling clays compared to the interactions with the layer, but the cation mobility is limited since the hydration process is not complete. This means that the heavy metal ions, present in the interlayer space, will be unable to diffuse and will be captured in the case of a low layer charge

Standard Operating Procedure - Collaborative Spatial Assessment CoSA - Release 1.0

The purpose of this Standard Operating Procedure (SOP) is to establish uniform procedures pertaining to the preparation for, the performance of, and the reporting of COllaborative (geo) Spatial Assessment (CoSA). CoSA provides a synoptic, unbiased assessment over the impact area of a disaster, which feeds the two main recovery perspectives of the Post-Disaster Needs Assessment (PDNA): i) the valuation of damages and losses carried out through the Damage and Loss Assessment (DaLA) methodology; and ii) the identification of human impacts and recovery needs carried out though the Human Recovery Needs Assessment (HRNA). CoSA is distinct from other geospatial and remote sensing based assessments because it i) draws on the collaborative efforts of distributed capacities in remote sensing and geospatial analysis, ii) aims to achieve the highest possible accuracy in line with the requirements of the PDNA and iii) tries to do so under stringent timing constraints set by the PDNA schedule. The current SOP will aid in ensuring credibility, consistency, transparency, accuracy and completeness of the CoSA. It is a living document, however, that will be enriched with new practical experiences and regularly updated to incorporate state-of-the-art procedures and new technical developments.JRC.DG.G.2-Global security and crisis managemen