Parámetros reproductivos en el ganado vacuno de raza retinta explotado en dehesa: edad al primer parto e intervalo entre partos

La mejora de los caracteres reproductivos es de vital importancia para maximizar esta rentabilidad de las explotaciones de vacuno de carne, especialmente en las razas explotadas en régimen extensivo, como es el caso de la raza Retinta. Dicho sistema supone condiciones adversas e incluso extremas para los animales, especialmente durante la fase de recría fase fundamental y a la que generalmente se presta escasa atención en estas explotaciones. El objetivo del presente trabaja fue, analizar la posible influencia de distintos factores, tanto ambientales como genéticos, sobre la edad al primer parto (EPP) y el intervalo entre partos (IPP). Para ello hemos contando con los registros reproductivos correspondientes al Núcleo de Control de Rendimientos de la Raza Retinta. Estos incluían 2.786 primeros partos y 11.197 intervalos entre partos, correspondientes a 3. 112 vacas. Según nuestros resultados la edad al primer parto del ganado vacuno de raza Retinta se ve afectada por los factores ganadería de nacimiento, campaña y época de nacimiento, y la interacción de los tres. Dentro de estos factores, el factor ganadería absorbió del 52 al 66% de la variabilidad de la edad al primer parto. Otro factor que resulto significativo fue el tipo de alimentación que recibe la ternera durante el periodo predestete. Así mismo nuestros resultados muestras que dentro del rango de EPP estudiadas, no existe influencia sobre las condiciones de crecimiento del ternero de ese primer parto ni de los posteriores, incluso las novillas que paren a edad temprana (dentro de unos límites fisiológicos), produjeron más terneros y más pesados durante su vida. En cuanto al periodo medio entre partos encontramos una fuerte relación con la ganadería, y con el efecto campaña ganadera, correspondiendo los intervalos más largos a aquellos años en los que aparece un notable período de sequía. Independientemente a este factor, se aprecia una evidente disminución del IPP en la última década. El valor medio obtenido está fuertemente influenciado por el sistema de cubrición seguido (paridera restringida o continua). Según nuestros resultados las ganaderías con temporada de cubrición continua presentan menores intervalos, aunque con bajas diferencias, lo cual puede ser refleja de un manejo reproductivo menos optimizado que las de paridera estacional

Clonación de genotipos adultos y juveniles de Quercus suber y Q. ilex tolerantes a Phytophthora cinnamomi

El síndrome de “la seca” está generando desde hace décadas la pérdida de masas de Quercus mediterráneos provocando grandes pérdidas económicas y ecológicas. Por ello, en 2019 el subgrupo de “Mejora Genética y Fisiología” del Grupo de Trabajo sobre Seca del Ministerio para la Transición Ecológica y el Reto Demográfico impulsó el Programa Nacional para la Conservación y Mejora de Recursos Genéticos de Encina y Alcornoque. Este programa contempla la clonación y la conservación de material tolerante a Phytophthora cinnamomi y/o sequía, seleccionado en anteriores proyectos de investigación. Aplicando técnicas de cultivo in vitro, embriogénesis somática y proliferación de yemas axilares, se pretende clonar una selección de individuos dentro de las progenies más tolerantes, y los mejores genotipos adultos evaluados a través de esas progenies. Las hojas son el explanto elegido para inducir embriones somáticos en material juvenil y adulto de alcornoque; en material de encina adulto el explanto inicial es el tegumento del embrión inmaduro. Las líneas embriogénicas generadas han sido conservadas a largo plazo mediante su crioconservación (almacenamiento en nitrógeno líquido). La proliferación de yemas axilares se emplea para clonar genotipos juveniles de ambas especies. La clonación del material permitirá disponer de copias suficientes que permitan la evaluación de su tolerancia en ensayos de campo

Phenotype and genotype of 87 patients with Mowat–Wilson syndrome and recommendations for care

Purpose: Mowat–Wilson syndrome (MWS) is a rare intellectual disability/multiple congenital anomalies syndrome caused by heterozygous mutation of the ZEB2 gene. It is generally underestimated because its rarity and phenotypic variability sometimes make it difficult to recognize. Here, we aimed to better delineate the phenotype, natural history, and genotype–phenotype correlations of MWS. Methods: In a collaborative study, we analyzed clinical data for 87 patients with molecularly confirmed diagnosis. We described the prevalence of all clinical aspects, including attainment of neurodevelopmental milestones, and compared the data with the various types of underlying ZEB2 pathogenic variations. Results: All anthropometric, somatic, and behavioral features reported here outline a variable but highly consistent phenotype. By presenting the most comprehensive evaluati

Action of chlorhexidine, zingiber officinale, and calcium hydroxide on candida albicans, enterococcus faecalis, escherichia coli, and endotoxin in the root canals

Made available in DSpace on 2019-09-12T16:26:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2016Aim: The purpose of this in vitro study was to evaluate the antimicrobial activity of 2% chlorhexidine gel (CHX) as auxiliary chemical substance and intracanal medications on Candida albicans, Enterococcus faecalis, Escherichia coli, and their endotoxins in the root canals. Materials and methods: The study was conducted on 48 single-rooted human teeth divided into four groups (n = 12), according to intracanal medications used: (1) Calcium hydroxide + apyrogenic saline solution (Ca(OH)2 + SS), (2) 20% ginger glycolic extract (GEN), (3) calcium hydroxide + 20% ginger glycolic extract (Ca(OH)2 + GEN), (4) apyrogenic SS (control). Collections were made from the root canal content before preparation (baseline-S1), immediately after instrumentation (S2), 7 days after instrumentation (S3), after 14 days the action of intracanal medication (S4), and 7 days after removal of the intracanal medication (S5). The antimicrobial activity and endotoxin content were analyzed for all collections. The results were statistically analyzed by the Kruskal-Wallis and Dunn tests at a significance level of 5%. Results: After instrumentation with CHX, there was complete elimination of E. coli and C. albicans, except for E. faecalis, which was significantly reduced and then completely eliminated after intracanal medication. There was significant reduction of endotoxin after instrumentation. Comparison of collection after instrumentation and intracanal medication revealed reduction of endotoxins in all groups; this reduction was greater in group Ca(OH)2 followed by the group GEN. Conclusion: It was concluded that the instrumentation using CHX and intracanal medication used were able to eliminate the microorganisms from the root canal; the endotoxins were reduced, yet not completely eliminated.Valera, M.C., Department of Restorative Dentistry, Institute of Science and Technology, UNESP-Univ Estadual Paulista, São José dos Campos, São Paulo, BrazilOliveira, S.A.C., Department of Restorative Dentistry, Institute of Science and Technology, UNESP-Univ Estadual Paulista, São José dos Campos, São Paulo, BrazilMaekawa, L.E., Department of Restorative Dentistry, Institute of Science and Technology, UNESP-Univ Estadual Paulista, São José dos Campos, São Paulo, BrazilCardoso, F.G.R., Division of Endodontics, Department of Odontology, University of Taubaté, Taubaté, São Paulo, BrazilChung, A., Department of Restorative Dentistry, Institute of Science and Technology, UNESP-Univ Estadual Paulista, São José dos Campos, São Paulo, BrazilSilva, S.F.P., Department of Restorative Dentistry, Institute of Science and Technology, UNESP-Univ Estadual Paulista, São José dos Campos, São Paulo, BrazilCarvalho, C.A.T., Department of Restorative Dentistry, Institute of Science and Technology, UNESP-Univ Estadual Paulista, São José dos Campos, São Paulo, Brazi

PPI1, UNA NUOVA PROTEINA CAPACE DI INTERAGIRE CON IL DOMINIO C-TERMINALE DELL' H+-ATPASI DEL PLASMALEMMA

Il dominio autoinibitorio C-terminale dell' H+-ATPasi del plasmalemma costituisce il principale sito di regolazione dell'enzima: la sua rimozione proteolitica, cos\uec come il legame di proteine 14-3-3 ad una sequenza contenuta in questa regione, causano un aumento dell'attivit\ue0 enzimatica. Utilizzando il metodo del doppio ibrido abbiamo identificato una proteina di 612 aa (Ppi1, proton pump interactor), la cui porzione N-terminale (88aa) interagisce con il dominio C-terminale dell'H+-ATPasi, isoforma AHA1. Diversi geni di Arabidopsis thaliana e alcuni EST di diverse specie vegetali mostrano una omologia di sequenza significativa (50-70% in porzioni di 200-600 aa) con Ppi1. La regione N-terminale di Ppi1 \ue8 stata espressa in E. coli come proteina di fusione con la GST o con una coda di istidine (His6-Ppi). In esperimenti di overlay, entrambe le proteine di fusione si legano all'H+-ATPasi immunoprecipitata da una frazione di plasmalemma purificata da cellule in coltura di A. thaliana His6-Ppi stimola l'attivit\ue0 dell'H+-ATPasi; lo stimolo \ue8 maggiore a pH 6.4 (massimo stimolo a 20 mM His-Ppi) che a pH 7.3, dove lo stimolo non \ue8 saturato neppure alla concentrazione di 60 mM. L'attivazione indotta da Ppi1 \ue8 sinergica con quella indotta da fusicoccina e da tripsina; inoltre His6-Ppi si lega all'H+-ATPasi trattata con tripsina. Il legame dell'attivatore avviene perci\uf2 ad un sito diverso da quello delle 14-3-3 e a monte del sito di taglio della tripsina

Correlation between volume of root canal, cultivable bacteria, bacterial complexes and endotoxins in primary infection

Made available in DSpace on 2019-09-11T20:51:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2019Universidade Estadual PaulistaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)This clinical study was conducted to correlate the levels of endotoxins and culturable bacteria found in primary endodontic infection (PEI) with the volume of root canal determined by using Cone Beam Computed Tomography (CBCT); and to evaluate the bacterial diversity correlating with clinical features. Twenty patients with PEI were selected and clinical features were recorded. The volume (mm3) of root canal was determined by CBCT analysis. Root canal samples were analyzed by using kinetic LAL-assay test to determine the levels of endotoxins and anaerobic technique to determine the bacterial count (CFU/mL). DNA was extracted from all samples to determine bacterial diversity and quantified by using Checkerboard-DNA-DNA- Hybridization. Culturable bacteria and endotoxins were detected in 100% of the root canal samples. Linear regression analysis revealed a correlation between root canal volume and presence of anaerobic bacteria (p<0.05). Positive correlations were found between bacteria species and presence of different clinical features (p<0.05). After grouping the bacteria species into bacterial complexes, positive associations were found between green, orange and red complexes with presence of sinus tract (p<0.05). This clinical study revealed that larger root canals hold higher levels of culturable bacteria in PEI. Thus, the interaction of different virulent bacteria species in complexes seems to play an important role in the development of clinical features. © 2019, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.Cardoso, F.G.R., Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos CamposSP, Brazil, Department of Dentistry, Endodontic Division, University of Taubaté – UNITAU, Taubaté, SP, BrazilMartinho, F.C., Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos CamposSP, Brazil, Department of Advanced Oral Science & Therapeutics, Endodontic Division, University of Maryland, School of DentistryMD, United StatesFerreira, N.S., Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos CamposSP, Brazil, Department of Semiology, UFPel - Universidade Federal de Pelotas, School of Dentistry, Pelotas, RS, Brazildo Prado, R.F., Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos CamposSP, BrazilManhães-Júnior, L.R.C., Department of Radiology, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos, SP, BrazilRocco, M.A., Department of Radiology, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos, SP, BrazilValera, M.C., Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos CamposSP, Brazi

Phenotypic characterization of pink pigmented facultative methylothrophic (PPFM) bacteria from various sources

A total of eighteen (18) pink-pigmented facultative methylotrophic (PPFM) bacteria from various habitats were isolated and described. A mineral salt agar medium with 0.5% methanol was used as the selective medium for the isolation. Pure cultures were obtained after re-streaking in selective as well as non-selective media. The obtained isolated were gram-negative rods with some unstained cyst like structures. The cyst like structures stained black after polyß-hydroxybutyrate staining with Sudan Black. All of the isolates grew in brain heart infusion agar, nutrient agar, glycerol peptone agar, and eosin methylene blue agar but not in MacConkey agar. Optimum incubation temperature for the isolates varied from room temperature (250C) and 370C. The isolates exhibited varied biological activities based on the different biochemical tests performed