51 research outputs found
Teleparallel Versions of Friedmann and Lewis-Papapetrou Spacetimes
This paper is devoted to investigate the teleparallel versions of the
Friedmann models as well as the Lewis-Papapetrou solution. We obtain the tetrad
and the torsion fields for both the spacetimes. It is shown that the
axial-vector vanishes for the Friedmann models. We discuss the different
possibilities of the axial-vector depending on the arbitrary functions
and in the Lewis-Papapetrou metric. The vector related with spin has
also been evaluated.Comment: 13 pages, accepted for publication in GR
Uso da fish em mucosa oral para investigação de mosaicismo com linhagem 45,x: estudo com homens saudĂĄveis e pacientes com distĂșrbios da diferenciação do sexo
FAPESP - FUNDAĂĂO DE AMPARO Ă PESQUISA DO ESTADO DE SĂO PAULOObjective: To verify whether fluorescence in situ hybridization (FISH) of cells from the buccal epithelium could be employed to detect cryptomosaicism with a 45,X lineage in 46,XY patients. Subjects and methods: Samples of nineteen 46,XY healthy young men and five patients with disorders of sex development (DSD), four 45,X/46,XY and one 46,XY were used. FISH analysis with X and Y specific probes on interphase nuclei from blood lymphocytes and buccal epithelium were analyzed to investigate the proportion of nuclei containing only the signal of the X chromosome. Results: The frequency of nuclei containing only the X signal in the two tissues of healthy men did not differ (p = 0.69). In all patients with DSD this frequency was significantly higher, and there was no difference between the two tissues (p = 0.38), either. Conclusions: Investigation of mosaicism with a 45,X cell line in patients with 46,XY DSD or sterility can be done by FISH directly using cells from the buccal epithelium. © ABE&M todos os direitos reservados.To verify whether fluorescence in situ hybridization (FISH) of cells from the buccal epithelium could be employed to detect cryptomosaicism with a 45,X lineage in 46,XY patients. Subjects and methods: Samples of nineteen 46,XY healthy young men and five patients with disorders of sex development (DSD), four 45,X/46,XY and one 46,XY were used. FISH analysis with X and Y specific probes on interphase nuclei from blood lymphocytes and buccal epithelium were analyzed to investigate the proportion of nuclei containing only the signal of the X chromosome. Results: The frequency of nuclei containing only the X signal in the two tissues of healthy men did not differ (p = 0.69). In all patients with DSD this frequency was significantly higher, and there was no difference between the two tissues (p = 0.38), either. Conclusions: Investigation of mosaicism with a 45,X cell line in patients with 46,XY DSD or sterility can be done by FISH directly using cells from the buccal epithelium584328334FAPESP - FUNDAĂĂO DE AMPARO Ă PESQUISA DO ESTADO DE SĂO PAULOFAPESP - FUNDAĂĂO DE AMPARO Ă PESQUISA DO ESTADO DE SĂO PAULO2011/50189-7Verificar se a hibridização in situ por fluorescĂȘncia (FISH) em cĂ©lulas de mucosa oral poderia ser empregada para detectar criptomosaicismo com linhagem 45,X em pacientes 46,XY. Sujeitos e mĂ©todos: Amostra de 19 jovens saudĂĄveis 46,XY e cinco pacientes com distĂșrbios da diferenciação do sexo (DDS), quatro 45,X/46,XY e um 46,XY. FISH com sondas especĂficas para X e Y em nĂșcleos interfĂĄsicos de linfĂłcitos e mucosa oral para investigar aproporção de nĂșcleos contendo apenas o sinal do cromossomo X. Resultados: A frequĂȘncia de nĂșcleos contendo apenas o sinal do X nos dois tecidos dos homens saudĂĄveis nĂŁo diferiu (p =0,69). Em todos os pacientes com DDS essa frequĂȘncia foi significativamente maior, e tambĂ©mnĂŁo houve diferença entre os dois tecidos (p = 0,38). ConclusĂ”es: A investigação de mosaicismo com linhagem 45,X em pacientes com DDS 46,XY ou esterilidade pode ser feita por FISHdiretamente em cĂ©lulas de mucosa ora
Teleparallel Energy-Momentum Distribution of Spatially Homogeneous Rotating Spacetimes
The energy-momentum distribution of spatially homogeneous rotating spacetimes
in the context of teleparallel theory of gravity is investigated. For this
purpose, we use the teleparallel version of Moller prescription. It is found
that the components of energy-momentum density are finite and well-defined but
are different from General Relativity. However, the energy-momentum density
components become the same in both theories under certain assumptions. We also
analyse these quantities for some special solutions of the spatially
homogeneous rotating spacetimes.Comment: 12 pages, accepted for publication in Int. J. Theor. Phy
Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum
The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ± 0.3%, respectively. We observed that the vasodilation action of EEAP was partially mediated by nitric oxide (·NO). The IAP showed the presence of calcium (137.76 ± 4.08 Όg mg-1). The EEAP and IAP showed an AHE in WKY and SHR animals. EEAP did not have cytotoxic effects or cause chromosomic alterations. The AHE shown by EEAP could result from its endothelium-dependent vascular action. Rutin-like flavonoids, among other polyphenols, could contribute to these biological activities, and the calcium present in EEAP could act in a synergistic way
DL5o da bactéria Aeromonas hydrophila para o matrinxã, Brycon amazonicus
In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxĂŁ, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutionsT1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxĂŁ is 6.66 x 10(11) cells/ mL.Para determinar a dose letal (DL50 96-h) da bactĂ©ria Aeromonas hydrophila para o matrinxĂŁ, Brycon amazonicus, com aplicabilidade para testes de desafio, foram utilizados 90 peixes (63,23 ± 6,39 g), divididos em cinco tratamentos, com diferentes soluçÔes bacterianas: T1 - Controle (solução salina 0,9% NaCl); T2 (4 x 10(11) cĂ©lulas/ mL); T3 (5 x 10(11) cĂ©lulas/ mL-1); T4 (1,36 x 10(12) cĂ©lulas/mL-1) e T5 (3,06 x 10(12) cĂ©lulas/ mL-1). Os peixes foram previamente anestesiados com benzocaĂna (60 mg L-1), inoculados na cavidade peritoneal com as suspensĂ”es bacterianas e distribuĂdos em 15 aquĂĄrios de vidro de 80 L de capacidade, com aeração constante. O experimento teve duração de 96 h, no qual foram monitoradas a mortalidade e a qualidade da ĂĄgua. O delineamento experimental foi inteiramente casualisado com trĂȘs rĂ©plicas e a DL50 96-h foi estimada de acordo com o mĂ©todo Spearman-Karber. Durante o experimento os parĂąmetros fĂsico-quĂmicos da ĂĄgua permaneceram dentro das condiçÔes consideradas adequadas para o desenvolvimento e saĂșde dos organismos aquĂĄticos. A mortalidade dos peixes aumentou nas concentraçÔes crescentes de A. hydrophila (T1 = 0%; T2 = 16,66%; T3 = 44,44%; T4 = 72,22% e T5 = 100%), contudo, as primeiras mortalidades ocorreram em 57 h apĂłs a inoculação das concentraçÔes bacterianas, sendo observados os primeiros sinais de infecção em 24 h apĂłs a inoculação. Os resultados indicam que o valor da DL50 96-h da bactĂ©ria A. hydrophila para o matrinxĂŁ foi igual a 6,66 x 10(11) cĂ©lulas/mL de solução salina
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