272 research outputs found
Crafting genetic diversity: unlocking the potential of protein evolution
Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and the flourishing vitality of living organisms, serving as the cornerstone for robust ecosystems and the continuous evolution of life on Earth. The landscape of directed evolution, a powerful biotechnological tool inspired by natural evolutionary processes, has undergone a transformative shift propelled by innovative strategies for generating genetic diversity. This shift is fuelled by several factors, encompassing the utilization of advanced toolkits like CRISPR-Cas and base editors, the enhanced comprehension of biological mechanisms, cost-effective custom oligo pool synthesis, and the seamless integration of artificial intelligence and automation. This comprehensive review looks into the myriad of methodologies employed for constructing gene libraries, both in vitro and in vivo, categorized into three major classes: random mutagenesis, focused mutagenesis, and DNA recombination. The objectives of this review are threefold: firstly, to present a panoramic overview of recent advances in genetic diversity creation; secondly, to inspire novel ideas for further innovation in genetic diversity generation; and thirdly, to provide a valuable resource for individuals entering the field of directed evolution
Experiments of Interfacial Roughening in Hele-Shaw Flows with Weak Quenched Disorder
We have studied the kinetic roughening of an oil--air interface in a forced
imbibition experiment in a horizontal Hele--Shaw cell with quenched disorder.
Different disorder configurations, characterized by their persistence length in
the direction of growth, have been explored by varying the average interface
velocity v and the gap spacing b. Through the analysis of the rms width as a
function of time, we have measured a growth exponent beta ~= 0.5 that is almost
independent of the experimental parameters. The analysis of the roughness
exponent alpha through the power spectrum have shown different behaviors at
short (alpha_1) and long (alpha_2) length scales, separated by a crossover
wavenumber q_c. The values of the measured roughness exponents depend on
experimental parameters, but at large velocities we obtain alpha_1 ~= 1.3
independently of the disorder configuration. The dependence of the crossover
wavenumber with the experimental parameters has also been investigated,
measuring q_c ~ v^{0.47} for the shortest persistence length, in agreement with
theoretical predictions.Comment: 20 pages, 22 figure
Optical activity of neutrinos and antineutrinos
Using the one-loop helicity amplitudes for low-energy
and scattering in the standard model with
massless neutrinos, we study the optical activity of a sea of neutrinos and
antineutrinos. In particular, we estimate the values of the index of refraction
and rotary power of this medium in the absence of dispersion.Comment: Additional reference
Conserving and Gapless Approximations for an Inhomogeneous Bose Gas at Finite Temperatures
We derive and discuss the equations of motion for the condensate and its
fluctuations for a dilute, weakly interacting Bose gas in an external potential
within the self--consistent Hartree--Fock--Bogoliubov (HFB) approximation.
Account is taken of the depletion of the condensate and the anomalous Bose
correlations, which are important at finite temperatures. We give a critical
analysis of the self-consistent HFB approximation in terms of the
Hohenberg--Martin classification of approximations (conserving vs gapless) and
point out that the Popov approximation to the full HFB gives a gapless
single-particle spectrum at all temperatures. The Beliaev second-order
approximation is discussed as the spectrum generated by functional
differentiation of the HFB single--particle Green's function. We emphasize that
the problem of determining the excitation spectrum of a Bose-condensed gas
(homogeneous or inhomogeneous) is difficult because of the need to satisfy
several different constraints.Comment: plain tex, 19 page
Extension of the sum rule for the transition rates between multiplets to the multiphoton case
The sum rule for the transition rates between the components of two
multiplets, known for the one-photon transitions, is extended to the
multiphoton transitions in hydrogen and hydrogen-like ions. As an example the
transitions 3p-2p, 4p-3p and 4d-3d are considered. The numerical results are
compared with previous calculations.Comment: 10 pages, 4 table
Upper critical field in dirty two-band superconductors: breakdown of the anisotropic Ginzburg-Landau theory
We investigate the upper critical field in a dirty two-band superconductor
within quasiclassical Usadel equations. The regime of very high anisotropy in
the quasi-2D band, relevant for MgB, is considered. We show that strong
disparities in pairing interactions and diffusion constant anisotropies for two
bands influence the in-plane in a different way at high and low
temperatures. This causes temperature-dependent anisotropy, in
accordance with recent experimental data in MgB. The three-dimensional
band most strongly influences the in-plane near , in the
Ginzburg-Landau (GL) region. However, due to a very large difference between
the c-axis coherence lengths in the two bands, the GL theory is applicable only
in an extremely narrow temperature range near . The angular dependence of
deviates from a simple effective-mass law even near .Comment: 12 pages, 5 figures, submitted to Phys.Rev.
First Demonstration of a Higher Order Depolarizing Resonance
This research was sponsored by the National Science Foundation Grant NSF PHY-931478
Siberian Snake Overcomes "Overlapping" Depolarizing Resonances
This research was sponsored by the National Science Foundation Grant NSF PHY-931478
RF Induced Depolarizing Resonanaces, Spin Flip, and Partial Siberian Snakes
This research was sponsored by the National Science Foundation Grant NSF PHY-931478
Nucleon charge exchange on the deuteron: A critical review
The existing experimental data on the d(n,p)nn and d(p,n)pp cross sections in
the forward direction are reviewed in terms of the Dean sum rule. It is shown
that the measurement of the ratio of the charge exchange on the deuteron to
that on the proton might, if taken together with other experimental data, allow
a direct construction of the np -> np scattering amplitude in the backward
direction with few ambiguities.Comment: 7 pages with 3 figure
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