Thymocyte Proliferation and Apoptosis Induced by Ionizing Radiation

Proliferation and apoptosis of rat and mouse thymocytes caused by ionizing radiation were studied. The percentage of proliferating cells was determined by the method of colchicine metaphases and the apoptosis was estimated as DNA fragmentation. In vitro irradiation with 0.05-0.2 Gy was found to stimulate thymocyte proliferation, the maximum was observed at 0.05 Gy for mouse thymocytes and at 0.1 Gy for rat thymocytes. These doses caused a slight decrease in DNA fragmentation, as compared to control. By raising the radiation dose, proliferation was reduced and DNA fragmentation was increased. The results obtained indicate that low radiation doses stimulate cell proliferation while higher doses trigger apoptosis of thymocytes

Localized charged states and phase separation near second order phase transition

Localized charged states and phase segregation are described in the framework of the phenomenological Ginzburg-Landau theory of phase transitions. The Coulomb interactions determines the charge distribution and the characteristic length of the phase separated states. The phase separation with charge segregation becomes possible because of the large dielectric constant and the small density of extra charge in the range of charge localization. The phase diagram is calculated and the energy gain of the phase separated state is estimated. The role of the Coulomb interaction is elucidated

Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region

The purpose of the research is the assessment of the Francisella tularensis occurrence in nature in ticks of the genus Dermacentor; understanding the physiological age in terms of tick infection with tularemia pathogen.Materials and methods. For the period from 2015 to 2019, we examined 8449 specimens of Dermacentor marginatus (916 pools), 8674 specimens of D. reticulatus (705 pools), and 109 specimens of D. niveus (40 pools) for tularemia infection. To assess the dependence of tularemia pathogen found in ticks of different physiological ages, we examined 2440 specimens of D. marginatus (360 pools), and 3349 specimens of D. reticulatus (412 pools) for the period from 2016 to 2019. Studies of ixodid ticks infected with tularemia pathogen were performed by the Natural Focal Infection Laboratory of the Stavropol Anti-Plague Institute. Pools of ixodid ticks were examined for the pathogen DNA of tularemia using reagent kits for identifying Francisella tularensis DNA by polymerase chain reaction with fluorescence hybridization of results recorded in real time.Results and discussion. The infection rate of the tularemia pathogen in ticks in the Central Pre-Caucasian region ranged from 0.044–1.127% in D. marginatus and 0.035–1.455% in D. reticulatus in different years. The greatest number of F. tularensis was isolated from the III physiological age ticks. For D. reticulatus ticks, no statistically significant dependence of the detected tularemia pathogen on physiological age was found

LHC Abort Gap Cleaning with the Transverse Damper

In the Large Hadron Collider, LHC, particles not captured by the RF system at injection or leaking out of the RF bucket may quench the superconducting magnets during beam abort. The problem, common to other superconducting machines, is particularly serious for the LHC due to the very large stored energy in the beam. For the LHC a way of removing the unbunched beam has been studied and it uses the existing damper kickers to excite resonantly the particles travelling along the abort gap. In this paper we describe the results of simulations performed with MAD X for various LHC optics configurations, including the estimated multipolar errors

СУЧАСНІ ТЕХНОЛОГІЇ ДИСТАНЦІЙНОГО НАВЧАННЯ В ПІСЛЯДИПЛОМНІЙ ОСВІТІ ЛІКАРІВ

Methods of distant education (DE) allow optimizing usage of human and financial resources. T. B. Andreazzi et al. (2011) underline importance of DE in countries with big territory and uneven division of resources and population. Usage of modern technologies of DE (videoconference, webinar, web-platform for learning, specialized website, mailing list, multimedia educative-controlling system, network textbook) allows students not only to get information, but also to ask questions and receive answer in real time mode. Usage of computerized methods of DE is important for modern postgraduate education of doctors and needs wider implementation.Застосування засобів дистанційного навчання (ДН) дозволяє оптимізувати використання людських та фінансових ресурсів. T. B. Andreazzi et al. (2011) підкреслюють важливість ДН у країнах, що мають велику територію і нерівномірний розподіл ресурсів та популяції. Використання сучасних технологій ДН (відеоконференція, вебінар, веб-платформа для навчання, спеціалізований сайт, електронна розсилка, мультимедійна навчально-контролююча система, мережевий електронний підручник) дозволяє слухачам не тільки отримати інформацію, але й задати питання та почути відповідь у режимі реального часу. Застосування комп’ютеризованих методів ДН є необхідним для сучасної післядипломної освіти лікарів та потребує ширшого впровадження.