Fast fluorescence microscopy for imaging the dynamics of embryonic development

Live imaging has gained a pivotal role in developmental biology since it increasingly allows real-time observation of cell behavior in intact organisms. Microscopes that can capture the dynamics of ever-faster biological events, fluorescent markers optimal for in vivo imaging, and, finally, adapted reconstruction and analysis programs to complete data flow all contribute to this success. Focusing on temporal resolution, we discuss how fast imaging can be achieved with minimal prejudice to spatial resolution, photon count, or to reliably and automatically analyze images. In particular, we show how integrated approaches to imaging that combine bright fluorescent probes, fast microscopes, and custom post-processing techniques can address the kinetics of biological systems at multiple scales. Finally, we discuss remaining challenges and opportunities for further advances in this field

Product Data Management – Defining the Used Terms

Part 9: Knowledge EngineeringInternational audienceThe Product Data Management (PDM) system and its associated terminology have changed over the years. Product Lifecycle Management (PLM) has become the predominant system and tends to overshadow PDM. However, PDM remains relevant and is a system commonly used by design engineers; mainly as a storage place for drawings and a place where drawings can be found for further editing.To obtain full benefit from the PDM/PLM systems, precise definitions are required. Without such definitions, the systems cannot function as they should and they cannot be used optimally. Furthermore, shortcomings in definitions may lead to a situation where the engineering community is unaware of the kind of help the systems can offer.The main focus of this conference paper is definition of some of the terms inherent to PDM/PLM systems and their data

Belastungen der Arbeitnehmer und des naeheren Umfeldes durch Tetrachlorethen (Perchlorethylen) in chemischen Reinigungen Pilotstudie

TIB: FR 1002 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Two-Photon Excitation Microscopy and Its Applications in Neuroscience

Two-photon excitation (2PE) overcomes many challenges in fluorescence microscopy. Compared to confocal microscopy, 2PE microscopy improves depth penetration, owing to the longer excitation wavelength required and to the ability to collect scattered emission photons as a useful signal. It also minimizes photodamage because lower energy photons are used and because fluorescence is confined to the geometrical focus of the laser spot. 2PE is therefore ideal for high-resolution, deep-tissue, time-lapse imaging of dynamic processes in cell biology. Here, we provide examples of important applications of 2PE for in vivo imaging of neuronal structure and signals; we also describe how it can be combined with optogenetics or photolysis of caged molecules to simultaneously probe and control neuronal activity