Computing symmetry groups of polyhedra

Knowing the symmetries of a polyhedron can be very useful for the analysis of its structure as well as for practical polyhedral computations. In this note, we study symmetry groups preserving the linear, projective and combinatorial structure of a polyhedron. In each case we give algorithmic methods to compute the corresponding group and discuss some practical experiences. For practical purposes the linear symmetry group is the most important, as its computation can be directly translated into a graph automorphism problem. We indicate how to compute integral subgroups of the linear symmetry group that are used for instance in integer linear programming.Comment: 20 pages, 1 figure; containing a corrected and improved revisio

Proximity effect thermometer for local temperature measurements on mesoscopic samples

Using the strong temperature dependent resistance of a normal metal wire in proximity to a superconductor, we have been able to measure the local temperature of electrons heated by flowing a dc current in a metallic wire to within a few tens of millikelvin at low temperatures. By placing two such thermometers at different parts of a sample, we have been able to measure the temperature difference induced by a dc current flowing in the sample. This technique may provide a flexible means of making quantitative thermal and thermoelectric measurements on mesoscopic metallic samples

Superconducting proximity effect in the presence of strong spin scattering

We report measurements of the four terminal temperature dependent resistance of narrow Au wires implanted with ~100 ppm Fe impurities in proximity to superconducting Al films. The wires show an initial decrease in resistance as the temperature is lowered through the superconducting transition of the Al films, but then show an increase in resistance as the temperature is lowered further. In contrast to the case of pure Au wires in contact with a superconducting film, the resistance at the lowest temperatures rises above the normal state resistance. Analysis of the data shows that, in addition to contributions from magnetic scattering and electron-electron interactions, the temperature dependent resistivity shows a substantial contribution from the superconducting proximity effect, which exists even in the presence of strong spin scattering.Comment: 4 pages, 3 figure

A methylated lysine is a switch point for conformational communication in the chaperone Hsp90

Methylation of a conserved lysine in C-terminal domain of the molecular chaperone Hsp90 was shown previously to affect its in vivo function. However, the underlying mechanism remained elusive. Through a combined experimental and computational approach, this study shows that this site is very sensitive to sidechain modifications and crucial for Hsp90 activity in vitro and in vivo. Our results demonstrate that this particular lysine serves as a switch point for the regulation of Hsp90 functions by influencing its conformational cycle, ATPase activity, co-chaperone regulation, and client activation of yeast and human Hsp90. Incorporation of the methylated lysine via genetic code expansion specifically shows that upon modification, the conformational cycle of Hsp90 is altered. Molecular dynamics simulations including the methylated lysine suggest specific conformational changes that are propagated through Hsp90. Thus, methylation of the C-terminal lysine allows a precise allosteric tuning of Hsp90 activity via long distances. Methylation of a lysine residue in Hsp90 is a recently discovered post-translational modification but the mechanistic effects of this modification have remained unknown so far. Here the authors combine biochemical and biophysical approaches, molecular dynamics (MD) simulations and functional experiments with yeast and show that this lysine is a switch point, which specifically modulates conserved Hsp90 functions including co-chaperone regulation and client activation

Author Correction: A methylated lysine is a switch point for conformational communication in the chaperone Hsp90

An amendment to this paper has been published and can be accessed via a link at the top of the paper

Web-based participatory surveillance of infectious diseases: the Influenzanet participatory surveillance experience.

To overcome the limitations of the state-of-the-art influenza surveillance systems in Europe, we established in 2008 a European-wide consortium aimed at introducing an innovative information and communication technology approach for a web-based surveillance system across different European countries, called Influenzanet. The system, based on earlier efforts in The Netherlands and Portugal, works with the participation of the population in each country to collect real-time information on the distribution of influenza-like illness cases through web surveys administered to volunteers reporting their symptoms (or lack of symptoms) every week during the influenza season. Such a large European-wide web-based monitoring infrastructure is intended to rapidly identify public health emergencies, contribute to understanding global trends, inform data-driven forecast models to assess the impact on the population, optimize the allocation of resources, and help in devising mitigation and containment measures. In this article, we describe the scientific and technological issues faced during the development and deployment of a flexible and readily deployable web tool capable of coping with the requirements of different countries for data collection, during either a public health emergency or an ordinary influenza season. Even though the system is based on previous successful experience, the implementation in each new country represented a separate scientific challenge. Only after more than 5 years of development are the existing platforms based on a plug-and-play tool that can be promptly deployed in any country wishing to be part of the Influenzanet network, now composed of The Netherlands, Belgium, Portugal, Italy, the UK, France, Sweden, Spain, Ireland, and Denmark

The Effect of Strength and Plyometric Training on Functional Dance Performance in Elite Ballet and Modern Dancers

Background: Ballet and modern dance are both art forms that require technique, artistry, grace and precision. Both dance forms require a degree of strength and muscular endurance for optimal performance. It is not known what value strength or plyometric training may have on functional dance performance. Objective: To systematically review the effects of strength and/or plyometric training on functional dance performance in elite ballet and modern dancers. Methods: A systematic review of literature indexed in the following databases: Medline, CINAHL, SportsDiscus, Physiotherapy Evidence Database (PEDro) and PubMed was conducted. The quality of the studies was graded using the PEDro Scale. Results: Eight studies satisfied the eligibility criteria and were included in this review. The studies\u27 population age range was 19?27?years. Methodological scores based on the PEDro scale were 4 to 6 out of 10. All of the included studies (100%) scored 4 out of 10 or higher on the PEDro scale. Strength training resulted in significant improvements in jump height (P? Conclusion: Moderate evidence indicates that supplementary strength training interventions via traditional resistance training or whole-body vibration methods and plyometric training interventions may increase certain dance-performance measures such as jump height and general aesthetic facility without changing certain anthropometric measures in elite ballet and modern dancers

A Cryptic Frizzled Module in Cell Surface Collagen 18 Inhibits Wnt/β−Catenin Signaling

Collagens contain cryptic polypeptide modules that regulate major cell functions, such as cell proliferation or death. Collagen XVIII (C18) exists as three amino terminal end variants with specific amino terminal polypeptide modules. We investigated the function of the variant 3 of C18 (V3C18) containing a frizzled module (FZC18), which carries structural identity with the extracellular cysteine-rich domain of the frizzled receptors. We show that V3C18 is a cell surface heparan sulfate proteoglycan, its topology being mediated by the FZC18 module. V3C18 mRNA was expressed at low levels in 21 normal adult human tissues. Its expression was up-regulated in fibrogenesis and in small well-differentiated liver tumors, but decreased in advanced human liver cancers. Low FZC18 immunostaining in liver cancer nodules correlated with markers of high Wnt/β−catenin activity. V3C18 (Mr = 170 kD) was proteolytically processed into a cell surface FZC18-containing 50 kD glycoprotein precursor that bound Wnt3a in vitro through FZC18 and suppressed Wnt3a-induced stabilization of β−catenin. Ectopic expression of either FZC18 (35 kD) or its 50 kD precursor inhibited Wnt/β−catenin signaling in colorectal and liver cancer cell lines, thus downregulating major cell cycle checkpoint gatekeepers cyclin D1 and c-myc and reducing tumor cell growth. By contrast, full-length V3C18 was unable to inhibit Wnt signaling. In summary, we identified a cell-surface signaling pathway whereby FZC18 inhibits Wnt/β−catenin signaling. The signal, encrypted within cell-surface C18, is released by enzymatic processing as an active frizzled cysteine-rich domain (CRD) that reduces cancer cell growth. Thus, extracellular matrix controls Wnt signaling through a collagen-embedded CRD behaving as a cell-surface sensor of proteolysis, conveying feedback cues to control cancer cell fate