Seismic Behaviour of Multistorey RC Frames with vertical Mass Irregularities

The buildings with mass irregularity behave differently as compared to regular buildings. In the present study, a parameter called mass irregularity index has been proposed to quantify the mass irregularity. The proposed factor depends mainly upon magnitude and location of mass irregularity. Further the present study aims to modify the expression of time period proposed by IS 1893:2002 and relation between mass irregularity coefficient and time period has been evaluated. For present study a family of 108 frames with mass irregularity have been modelled and analyzed by time history analysis. The proposed expression for time period has been validated for buildings with mass irregularity

Identification of geospatial variability of fluoride contamination in ground water of Mathura District, Uttar Pradesh, India

Groundwater is one of the major sources of water in arid and semi-arid regions. Groundwater quality data and its spatial distribution are important for the purpose of planning and management. Geo-statistical methods are one of the most advanced techniques for interpolation of groundwater quality. In this study, kriging methods were used for predicting the spatial distribution of fluoride content in groundwater. Data were collected from 13 wells in Mathura district (Uttar Pradesh, India). After normalization of data, semivariogram was drawn, for selecting suitable model for fitness on experimental semivariogram, less residual sum of squares (RSS) value was used. Then fluoride endemic areas of the Mathura District (study area) were identified from developed semivariogram model and Geospatial variability (high and low fluoride containing areas) map was generated with the help of GeographicInformation System. In the analysis, spatial distribution characteristics and variation of fluoride concentration in shallow groundwater found to be 3.4 and 4.6 mg/l at Sahar, Shahpur were higher than the standard limits (1.5 mg/l) of drinking water and shows remarkable spatial variability

Effect of Flyash and Waste Rubber on Properties of Concrete composite

Increasing use of waste materials like flyash, scrap tyre rubber etc, in construction industry has reduced the handling and disposal problems of these wastes. Flyash and scrap tyre rubbers are generally, employed to develop light weight and low strength concrete composites. The present work discusses the influence of flyash and waste tyre rubber particles on the behaviour of concrete composite. The rubber content has been taken in the range of 0 to 40% as replacement of fine and coarse aggregates while the flyash has been varied from 0 to 30% for cement. Testing of the concrete specimen prepared under different percentage of flyash and rubber waste was performed at 28 days of age for workability, density, compressive and bond strength. Experimental results show that the density, compressive strength and bond strength decreases while workability increases with increasing rubber content. Addition of flyash also decreases the density and compressive strength

Fundamental time period of RC Setback Buildings

The buildings with the presence of setback irregularity are now being increasingly used in the urban areas. The present work proposes an irregularity index for quantifying the setback irregularity based on the dynamic characteristics of the buildings. This paper also proposes a modified equation for the fundamental period of vibration, for building frames with setback irregularity. Furthermore, the equations for estimating the maximum inter storey drift ratio (Ir) and maximum displacement ductility (μmax) are also proposed. These equations are proposed on basis of the regression analysis conducted on the seismic response databank of 305 building models with different types of setback irregularity for each height category. The proposed equations are represented as a function of the irregularity index, and are validated for 2D and 3D building models with setback irregularity

Lamin A/C Haploinsufficiency Modulates the Differentiation Potential of Mouse Embryonic Stem Cells

BACKGROUND: Lamins are structural proteins that are the major determinants of nuclear architecture and play important roles in various nuclear functions including gene regulation and cell differentiation. Mutations in the human lamin A gene cause a spectrum of genetic diseases that affect specific tissues. Most available mouse models for laminopathies recapitulate disease symptoms for muscle diseases and progerias. However, loss of human lamin A/C also has highly deleterious effects on fetal development. Hence it is important to understand the impact of lamin A/C expression levels on embryonic differentiation pathways. METHODOLOGY AND PRINCIPAL FINDINGS: We have investigated the differentiation potential of mouse embryonic stem cells containing reduced levels of lamin A/C by detailed lineage analysis of embryoid bodies derived from these cells by culture. We initially carried out a targeted disruption of one allele of the mouse lamin A/C gene (). Undifferentiated wild-type and embryonic stem cells showed similar expression of pluripotency markers and cell cycle profiles. Upon spontaneous differentiation into embryoid bodies, markers for visceral endoderm such as α-fetoprotein were highly upregulated in haploinsufficient cells. However, neuronal markers such as β-III tubulin and nestin were downregulated. Furthermore, we observed a reduction in the commitment of cells into the myogenic lineage, but no discernible effects on cardiac, adipocyte or osteocyte lineages. In the next series of experiments, we derived embryonic stem cell clones expressing lamin A/C short hairpin RNA and examined their differentiation potential. These cells expressed pluripotency markers and, upon differentiation, the expression of lineage-specific markers was altered as observed with embryonic stem cells. CONCLUSIONS: We have observed significant effects on embryonic stem cell differentiation to visceral endoderm, neuronal and myogenic lineages upon depletion of lamin A/C. Hence our results implicate lamin A/C level as an important determinant of lineage-specific differentiation during embryonic development

Trends in the epidemiology of larynx and lung cancer in south-east England, 1985–2004

We analysed data on 8987 larynx and 174060 lung cancer patients diagnosed between 1985 and 2004, of which 17.3% of larynx and 35.5% of lung cancers were in females. The age-standardised rates for each cancer declined in both sexes, but since the 1990s, the rates in females over 70 years of age have been diverging

Background measurements and detector response studies for ISMRAN experiment

We report the measurement of the non-reactor environmental backgrounds and the detector response with the Indian Scintillator Matrix for Reactor Anti-Neutrinos (ISMRAN), which is $\sim$1 ton detector setup by volume, consisting of 10$\times$9 (10 rows and 9 columns) Plastic Scintillator Bars (PSBs) array at BARC, Mumbai, India. ISMRAN is an above-ground anti-neutrino ($\mathrm{\overline\nu_{e}}$) experiment at very short baseline located at Dhruva research reactor facility. It is enclosed by a shielding made of 10 cm thick lead and 10 cm thick borated polyethylene to minimize the backgrounds and is mounted on a movable base structure, situated at $\sim$ 13 m away from the reactor core. These measurements are useful in the context of the ISMRAN detector setup that will be used to detect the reactor $\mathrm{\overline\nu_{e}}$ and measure its energy spectrum through the inverse beta decay (IBD) process. In this paper, we present the energy resolution model and energy non-linearity model of PSB and the cosmogenic muon-induced background, based on the sum of their energy depositions and number of hit bars. Reconstructed sum energy spectrum and number of hit bars distribution for $\mathrm{{}^{22}Na}$ radioactive source has been compared with Geant4 based Monte Carlo simulations. These experimentally measured results will be useful for discriminating the correlated and uncorrelated background events from the true IBD events in reactor ON and OFF conditions inside the reactor hall.Comment: 4 pages, 4 figures. arXiv admin note: text overlap with arXiv:2208.0349

Alien chromosome segment from Aegilops speltoides and Dasypyrum villosum increases drought tolerance in wheat via profuse and deep root system

BackgroundRecurrent drought associated with climate change is a major constraint to wheat (Triticum aestivum L.) productivity. This study aimed to (i) quantify the effects of addition/substitution/translocation of chromosome segments from wild relatives of wheat on the root, physiological and yield traits of hexaploid wheat under drought, and (ii) understand the mechanism(s) associated with drought tolerance or susceptibility in wheat-alien chromosome lines.MethodsA set of 48 wheat-alien chromosome lines (addition/substitution/translocation lines) with Chinese Spring background were used. Seedling root traits were studied on solid agar medium. To understand the influence of drought on the root system of adult plants, these 48 lines were grown in 150-cm columns for 65 d under full irrigation or withholding water for 58 d. To quantify the effect of drought on physiological and yield traits, the 48 lines were grown in pots under full irrigation until anthesis; after that, half of the plants were drought stressed by withholding water for 16 d before recording physiological and yield-associated traits.ResultsThe alien chromosome lines exhibited altered root architecture and decreased photochemical efficiency and seed yield and its components under drought. The wheat-alien chromosome lines T5DS5S#3L (TA5088) with a chromosome segment from Aegilops speltoides (5S) and T5DL(.)5V#3S (TA5638) with a chromosome segment from Dasypyrum villosum (5V) were identified as drought tolerant, and the drought tolerance mechanism was associated with a deep, thin and profuse root system.ConclusionsThe two germplasm lines (TA5088 and TA5638) could be used in wheat breeding programs to improve drought tolerance in wheat and understand the underlying molecular genetic mechanisms of root architecture and drought tolerance

A diploid wheat TILLING resource for wheat functional genomics

Citation: Rawat, N., . . . & Gill, B. (2012). A diploid wheat TILLING resource for wheat functional genomics. BMC Plant Biology, 12(1), 205. https://doi.org/10.1186/1471-2229-12-205Background: Triticum monococcum L., an A genome diploid einkorn wheat, was the first domesticated crop. As a diploid, it is attractive genetic model for the study of gene structure and function of wheat-specific traits. Diploid wheat is currently not amenable to reverse genetics approaches such as insertion mutagenesis and post-transcriptional gene silencing strategies. However, TILLING offers a powerful functional genetics approach for wheat gene analysis. Results: We developed a TILLING population of 1,532 M[subscript 2] families using EMS as a mutagen. A total of 67 mutants were obtained for the four genes studied. Waxy gene mutation frequencies are known to be 1/17.6 - 34.4 kb DNA in polyploid wheat TILLING populations. The T. monococcum diploid wheat TILLING population had a mutation frequency of 1/90 kb for the same gene. Lignin biosynthesis pathway genes- COMT1, HCT2, and 4CL1 had mutation frequencies of 1/86 kb, 1/92 kb and 1/100 kb, respectively. The overall mutation frequency of the diploid wheat TILLING population was 1/92 kb. Conclusion: The mutation frequency of a diploid wheat TILLING population was found to be higher than that reported for other diploid grasses. The rate, however, is lower than tetraploid and hexaploid wheat TILLING populations because of the higher tolerance of polyploids to mutations. Unlike polyploid wheat, most mutants in diploid wheat have a phenotype amenable to forward and reverse genetic analysis and establish diploid wheat as an attractive model to study gene function in wheat. We estimate that a TILLING population of 5, 520 will be needed to get a non-sense mutation for every wheat gene of interest with 95% probability