DSMC evaluation of the Navier-Stokes shear viscosity of a granular fluid

A method based on the simple shear flow modified by the introduction of a deterministic non-conservative force and a stochastic process is proposed to measure the Navier-Stokes shear viscosity in a granular fluid described by the Enskog equation. The method is implemented in DSMC simulations for a wide range of values of dissipation and density. It is observed that, after a certain transient period, the system reaches a hydrodynamic stage which tends to the Navier-Stokes regime for long times. The results are compared with theoretical predictions obtained from the Chapman-Enskog method in the leading Sonine approximation, showing quite a good agreement, even for strong dissipation.Comment: 6 pages, 4 figures; to appear in Rarefied Gas Dynamics: 24th International Symposium (AIP Conference Proceedings

Individual 17-Hydroxyprogesterone Responses to hCG Are Not Correlated With Follicle Size in Polycystic Ovary Syndrome.

Context:In women with polycystic ovary syndrome (PCOS), 17-hydroxyprogesterone (17-OHP) responses to gonadotropin stimulation vary from increased to indistinguishable compared with normal controls. Objective:To determine whether 17-OHP responses to recombinant-human chorionic gonadotropin (r-hCG) are individually correlated to the size of antral follicles among women with PCOS. Design Setting and Participants:A prospective study conducted in 19 women with PCOS and 20 normal controls at an academic medical center. Interventions:Blood samples were obtained before and 24 hours after administration of 25 μg of r-hCG. Ovarian imaging was conducted with three-dimensional pelvic ultrasonography. Each subject underwent a 2-hour oral glucose tolerance test. Main Outcome Measures:Basal and stimulated levels of 17-OHP, androgens, estradiol, progesterone, anti-Mullerian hormone (AMH), insulin, glucose, follicle number, and size. Results:In women with PCOS, mean antral follicle count (AFC) was greater than that of controls, although the size of cohort follicles within individual subjects was not correlated to 17-OHP responses. The numbers of 2- to 3-mm and 3- to 4-mm follicles in PCOS were significantly greater than in controls, whereas differences between larger follicles were not observed. Increased AMH in PCOS was correlated to AFC, but not 17-OHP responses. Insulin sensitivity did not correlate to r-hCG‒stimulated 17-OHP after adjustment for body mass index. Conclusions:17-OHP responses to hCG in individuals with PCOS were not correlated to the distribution of antral follicles. Greater numbers of small antral follicles in women with PCOS than in controls suggest an extension of accelerated growth from the preantral stage

Breakdown of Energy Equipartition in a 2D Binary Vibrated Granular Gas

We report experiments on the equipartition of kinetic energy between grains made of two different materials in a mixture of grains vibrated in 2 dimensions. In general, the two types of grains do not attain the same granular temperature, Tg = 1/2m v^2. However, the ratio of the two temperatures is constant in the bulk of the system and independent of the vibration velocity. The ratio depends strongly on the ratio of mass densities of the grains, but is not sensitive to the inelasticity of grains. Also, this ratio is insensitive to compositional variables of the mixture such as the number fraction of each component and the total number density. We conclude that a single granular temperature, as traditionally defined, does not characterize a multi-component mixture.Comment: 4 pages, 5 figures, submitted to Physical Review Letters, updated reference

A numerical study of the Navier-Stokes transport coefficients for two-dimensional granular hydrodynamics

A numerical study that aims to analyze the thermal mechanisms of unsteady, supersonic granular flow by means of hydrodynamic simulations of the Navier–Stokes granular equation is reported in this paper. For this purpose, a paradigmatic problem in granular dynamics such as the Faraday instability is selected. Two different approaches for the Navier–Stokes transport coefficients for granular materials are considered, namely the traditional Jenkins–Richman theory for moderately dense quasi-elastic grains and the improved Garzó–Dufty–Lutsko theory for arbitrary inelasticity, which we also present here. Both the solutions are compared with event-driven simulations of the same system under the same conditions, by analyzing the density, temperature and velocity field. Important differences are found between the two approaches, leading to interesting implications. In particular, the heat transfer mechanism coupled to the density gradient, which is a distinctive feature of inelastic granular gases, is responsible for a major discrepancy in the temperature field and hence in the diffusion mechanisms

Motor neuron differentiation of iPSCs obtained from peripheral blood of a mutant TARDBP ALS patient

Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative disease, mainly affecting the motor neurons (MNs) and without effective therapy. Drug screening is hampered by the lack of satisfactory experimental and pre-clinical models. Induced pluripotent stem cells (iPSCs) could help to define disease mechanisms and therapeutic strategies as they could be differentiated into MNs, otherwise inaccessible from living humans. In this study, given the seminal role of TDP-43 in ALS pathophysiology, MNs were obtained from peripheral blood mononuclear cells-derived iPSCs of an ALS patient carrying a p.A382T TARDBP mutation and a healthy donor. Venous samples were preferred to fibroblasts for their ease of collection and no requirement for time consuming extended cultures before experimentation. iPSCs were characterized for expression of specific markers, spontaneously differentiated into primary germ layers and, finally, into MNs. No differences were observed between the mutated ALS patient and the control MNs with most of the cells displaying a nuclear localization of the TDP-43 protein. In conclusion, we here demonstrated for the first time that human TARDBP mutated MNs can be successfully obtained exploiting the reprogramming and differentiation ability of peripheral blood cells, an easily accessible source from any patient

Golexanolone improves fatigue, motor incoordination and gait and memory in rats with bile duct ligation

\ua9 2023 The Authors. Liver International published by John Wiley & Sons Ltd.Background and Aims: Many patients with the chronic cholestatic liver disease primary biliary cholangitis (PBC) show fatigue and cognitive impairment that reduces their quality of life. Likewise, rats with bile duct ligation (BDL) are a model of cholestatic liver disease. Current PBC treatments do not improve symptomatic alterations such as fatigue or cognitive impairment and new, more effective treatments are therefore required. Golexanolone reduces the potentiation of GABAA receptors activation by neurosteroids. Golexanolone reduces peripheral inflammation and neuroinflammation and improves cognitive and motor function in rats with chronic hyperammonemia. The aims of the present study were to assess if golexanolone treatment improves fatigue and cognitive and motor function in cholestatic BDL rats and if this is associated with improvement of peripheral inflammation, neuroinflammation, and GABAergic neurotransmission in the cerebellum. Methods: Rats were subjected to bile duct ligation. One week after surgery, oral golexanolone was administered daily to BDL and sham-operated controls. Fatigue was analysed in the treadmill, motor coordination in the motorater, locomotor gait in the Catwalk, and short-term memory in the Y-maze. We also analysed peripheral inflammation, neuroinflammation, and GABAergic neurotransmission markers by immunohistochemistry and Western blot. Results: BDL induces fatigue, impairs memory and motor coordination, and alters locomotor gait in cholestatic rats. Golexanolone improves these alterations, and this was associated with improvement of peripheral inflammation, neuroinflammation, and GABAergic neurotransmission in the cerebellum. Conclusion: Golexanolone may have beneficial effects to treat fatigue, and motor and cognitive impairment in patients with the chronic cholestatic liver disease PBC

Hydrodynamics of driven granular gases

Hydrodynamic equations for granular gases driven by the Fokker-Planck operator are derived. Transport coefficients appeared in Navier-Stokes order change from the values of a free cooling state to those of a steady state.Comment: 5 pages, 3 figure