Exome sequencing from nanogram amounts of starting DNA: comparing three approaches

Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii) whole-genome amplification of DNA samples with REPLI-g (Qiagen) and GenomePlex (Sigma) kits followed by standard library preparation, and (iii) library construction with a low input oriented ThruPLEX kit (Rubicon Genomics). Exome capture with Agilent SureSelectXT2 Human AllExon v4+UTRs capture probes, and HiSeq2000 sequencing were performed for test libraries along with the control library prepared from 1 µg of starting DNA. Tested protocols were characterized in terms of mapping efficiency, enrichment ratio, coverage of the target region, and reliability of SNP genotyping. REPLI-g- and ThruPLEX-FD-based protocols seem to be adequate solutions for exome sequencing of low input samples

The application of modular technology of training in the model of dual vocational education

В статье рассматриваются вопросы применения дуальной системы в практике современного российского профессионального образования. Раскрываются возможности модульной технологии обучения в модели дуального профессионального образования студентов колледжа на примере компетенции «Промышленная автоматика»The article discusses the use of the dual system in practice of modern Russian professional education. Reveal the possibilities of modular technology education in the model of dual vocational education College students on the example of the competence of «Industrial automation

DEFINITION OF PRESSURE INTRAMYOCARDIAL AS A METHOD FOR EVALUATING MORPHOFUNCTIONAL STATE STRUCTURE OF THE HEART AT DIFFERENT STAGES OF ONTOGENESIS

Intramyocardial pressure structures of the heart is determined by qualitative and. quantita-tive characteristics of its constituent structural elements. Correlation analysis revealed that the formation of stress-strain states in addition to cardiomyocytes affect fibers of connective tissue

Comparison of the results of star counts in nine open star clusters by the data of 2MASS and Gaia DR1

Звездные подсчеты с помощью метода kernel estimator проведены для скоплений NGC 1912, NGC 2099, NGC 4052, NGC 5715, NGC 6268, NGC 6834, NGC 7142, IC 2714, Czernik 38. Для подсчетов были использованы данные каталога Gaia DR1. Полученные результаты сравниваются с результатами звездных подсчетов по данным каталога 2MASS.Star counts were carried out for the clusters NGC 1912, NGC 2099, NGC 4052, NGC 5715, NGC 6268, NGC 6834, NGC 7142, IC 2714, Czernik 38 with the use of kernel estimator by Gaia DR1 data. Results are compared with the results of star counts by 2MASS data.Часть работ проведена при финансовой поддержке государства в лице Министерства образования и науки Российской Федерации (базовая часть госзадания, РК №AAAA-A17-117030310283-7), а также при финансовой поддержке Правительства РФ (постановление №211, контракт №02.A03.21.0006)

Structural and dynamical characteristics of nine open star clusters

В рамках проекта по созданию однородного каталога структурных и динамических характеристик рассеянных звездных скоплений звездные подсчеты с помощью метода kernel estimator проведены для скоплений NGC 1912, NGC 2099, NGC 4052, NGC 5715, NGC 6268, NGC 6834, NGC 7142, IC 2714, Czernik 38. Для подсчетов были использованы данные каталога 2MASS. Для скоплений выборки построены карты распределения и радиальные профили поверхностной плотности, получены оценки функций светимости и функций масс, определены положения центров, получены оценки радиусов, чисел звезд и масс скоплений.Within the framework of the project of a development of the uniform catalog of structural and dynamical characteristics of open star clusters, star counts were carried out for the clusters NGC 1912, NGC 2099, NGC 4052, NGC 5715, NGC 6268, NGC 6834, NGC 7142, IC 2714, Czernik 38 with the use of kernel estimator by 2MASS data. Two-dimensional maps and radial profiles of a surface density, estimates of luminosity functions and mass functions have obtained for the sample clusters. The positions of cluster centres and estimates of the cluster radii, number of stars and masses have been determined.Часть работ проведена при финансовой поддержке государства в лице Министерства образования и науки Российской Федерации (базовая часть госзадания, РК № AAAA-A17-117030310283-7), а также при финансовой поддержке Правительства РФ (постановление № 211, контракт № 02.A03.21.0006)

Consideration of unresolved binaries with evaluation of the mass of star clusters

При различных предположениях о функции масс компонент двойных систем оценивается, как наличие неразрешенных двойных систем влияет на определение массы скопления.An influence of the presence of unresolved binaries onto star cluster mass estimation is evaluated with different assumptions on the mass function of binary components.Часть работ проведена при финансовой поддержке государства в лице Министерства образования и науки Российской Федерации (базовая часть госзадания, РК №AAAA-A17-117030310283-7), а также при финансовой поддержке Правительства РФ (постановление № 211, контракт № 02.A03.21.0006)

Encounter between Man and State: Crimes Caused by Emotions in 18th-Century Russia

Поступила в редакцию: 22.06.2023. Принята к печати: 07.05.2024.Submitted: 22.06.2023. Accepted: 07.05.2024.Рецензия на книгу: Каменский А. Б. Любовь, страсть и отчаяние — русские преступления XVIII века. — СПб. : Алетейя, 2022. — 406 с. В рецензии представлен анализ книги А. Б. Каменского «Любовь, страсть и отчаяние — русские преступления XVIII века». Автор посвятил свою работу теме борьбы государства и церкви в России раннего Нового времени, показав ее через изменение правовых норм, которые применялись преимущественно для регулирования сферы брачно-семейных отношений. В каждой главе монографии произведен анализ процесса криминализации таких порицавшихся обществом девиаций, как внебрачные связи, изнасилование, инцест, нарушения брачного законодательства, самоубийства и др. Одним из достоинств издания является богатый эмпирический материал, который прекрасно иллюстрирует основные тенденции в развитии русского права. В то же время обилие данных видится одной из слабых сторон исследования, так как нередко скрывает от читателя первоначальный замысел книги.Review of: Kamensky, A. B. (2022). Liubov’, strast’ i otchaianie — russkie prestupleniia XVIII veka [Love, Passion, and Despair — Russian Crimes of the 18th Century]. St Petersburg: Aletheia. 406 p. This review analyses A. B. Kamensky’s book Love, Passion and Despair — Russian Crimes in the 18th Century. The author devotes his work to the struggle between the state and the church in early modern Russia, showing this through the changing legal norms that were used primarily to regulate the sphere of marriage and family relations. Each chapter analyses the process of criminalisation of such socially reprehensible deviations as extramarital sexual relations, rape, incest, violation of marriage regulations, suicide and others. One of the merits of the publication is the wealth of empirical material that perfectly illustrates the main trends in the development of Russian law. At the same time, the abundance of data seems to be one of the shortcomings of the study, as it sometimes tends to conceal from the reader the original intention of the book

Regularities of structural-cellular structure of ovaries in ontogenesis

The article presents the examination of the ovaries of embryos, ovaries of human feti and ovaries of children of the tender age. The researches revealed the increase of the surface of follicles, nucleus and primary oocyte's cytoplasm that tells about the fact that further follicular maturation continues in the ovaries of a child of 2-4 months old. Also we registered the increase of nuclear cytoplasmic ratio, appearance of single mature follicles with cumulus oophorus that testifies to the fact that the ovary is functionally mature. Furthermore the research revealed increased content of lipids and DNA in follicular epithelium that also tells aboutfunctional maturity of the ovary. It was found that incretory activity of an ovary during the first year of life is provided by the cells originated from the connective tissue membrane of atresic follicles

Morphological estimation of changes in cancellous raduis bone for forensic age determination

Visual researches showed that the cancellous bone of the distal radius has significant morphological changes with the increase of years. Revealed structural changes of cancellous bone allows to differentiate the age of an object and to direct a medical expert towards the proposed age group at the identification study. As a result of photocolorimetric research of involution changes of cancellous bone of the distal radius we found statistically significant increase of light transmission with the increase of years which is associated with an increase of osteoporosis. The correlation coefficient ranged from 0,63 to 0,80. On the basis of these data mathematical models that allow to determine the person's age in the forensic identification researches of the distal radius were developed (standard mean square error in determination of the age was from 6,6 to 8,2 years)