Cryptic genetic structure in an Argentinian population of Anastrepha fraterculus (Diptera: Tephritidae) evidenced by SSR markers and quantitative traits

In some regions of Argentina and Brazil, the South American fruit fly Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) causes significant damage to crops. An efficient integrated management program requires knowledge of pest population dynamics, dispersion patterns, sexual and oviposition behaviour, and adaptive landscape. The present study combined simple sequence repeat (SSR) molecular markers and morphometric datasets in order to analyse the population structure and infer the oviposition resource use strategy of the females. Infested guava fruits were collected from nine wild trees in Tucumán, Argentina, and a total of 140 adult A. fraterculus were recovered. These were then measured for six morphometric traits and 89 of them were genotyped for eight SSR loci. Genetic variability estimates were high (expected heterozygosity = 0.71, allelic richness = 12.5), with 8 to 20 alleles per locus. According to Wright's F-statistics estimates, the highest proportion (83%) of genetic variation occurred within individuals while variance between and within fruits were similar (≈ 8.5%). Analysis of the cryptic genetic structure based on SSR using different approaches, namely discriminant analysis of principal components (DAPC) and sparse non-negative matrix factorization (SNMF), yielded results consistent with the occurrence of two clusters with virtually no admixture. Average kinship between individuals which had emerged from the same fruit (0.07) was lower than that expected for full-sib families. Univariate and multivariate analyses of phenotypic data showed 54-66% of variance among individuals within fruits and 34-46% among fruits. The comparison between phenotypic (PST) and molecular (FST) differentiation identified wing width and length as possible target of positive selection. The average kinship and high genetic variation within fruits, together with the highly significant genetic differentiation among fruits, supports the hypothesis that each fruit was colonised by about three ovipositing females. The results also indicate that females were able to disperse widely from the emergence site before mating and starting oviposition activity.Instituto de GenéticaFil: Rodriguez, Angeles I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Ferreyra, Laura Ines. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; ArgentinaFil: Gomez Cendra, Paula V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vilardi, Juan Cesar. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Beam-helicity asymmetries for single-hadron production in semi-inclusive deep-inelastic scattering from unpolarized hydrogen and deuterium targets

A measurement of beam-helicity asymmetries for single-hadron production in deep-inelastic scattering is presented. Data from the scattering of 27.6 GeV electrons and positrons off gaseous hydrogen and deuterium targets were collected by the HERMES experiment. The asymmetries are presented separately as a function of the Bjorken scaling variable, the hadron transverse momentum, and the fractional energy for charged pions and kaons as well as for protons and anti-protons. These asymmetries are also presented as a function of the three aforementioned kinematic variables simultaneously

Transverse-target-spin asymmetry in exclusive ω\omega-meson electroproduction

Hard exclusive electroproduction of $\omega$ mesons is studied with the HERMES spectrometer at the DESY laboratory by scattering 27.6 GeV positron and electron beams off a transversely polarized hydrogen target. The amplitudes of five azimuthal modulations of the single-spin asymmetry of the cross section with respect to the transverse proton polarization are measured. They are determined in the entire kinematic region as well as for two bins in photon virtuality and momentum transfer to the nucleon. Also, a separation of asymmetry amplitudes into longitudinal and transverse components is done. These results are compared to a phenomenological model that includes the pion pole contribution. Within this model, the data favor a positive $\pi\omega$ transition form factor.Comment: DESY Report 15-14

Longitudinal double-spin asymmetries in semi-inclusive deep-inelastic scattering of electrons and positrons by protons and deuterons

A comprehensive collection of results on longitudinal double-spin asymmetries is presented for charged pions and kaons produced in semi-inclusive deep-inelastic scattering of electrons and positrons on the proton and deuteron, based on the full HERMES data set. The dependence of the asymmetries on hadron transverse momentum and azimuthal angle extends the sensitivity to the flavor structure of the nucleon beyond the distribution functions accessible in the collinear framework. No strong dependence on those variables is observed. In addition, the hadron charge-difference asymmetry is presented, which under certain model assumptions provides access to the helicity distributions of valence quarks

Bose-Einstein correlations in hadron-pairs from lepto-production on nuclei ranging from hydrogen to xenon

Bose-Einstein correlations of like-sign charged hadrons produced in deep-inelastic electron and positron scattering are studied in the HERMES experiment using nuclear targets of $^1$H, $^2$H, $^3$He, $^4$He, N, Ne, Kr, and Xe. A Gaussian approach is used to parametrize a two-particle correlation function determined from events with at least two charged hadrons of the same sign charge. This correlation function is compared to two different empirical distributions that do not include the Bose-Einstein correlations. One distribution is derived from unlike-sign hadron pairs, and the second is derived from mixing like-sign pairs from different events. The extraction procedure used simulations incorporating the experimental setup in order to correct the results for spectrometer acceptance effects, and was tested using the distribution of unlike-sign hadron pairs. Clear signals of Bose-Einstein correlations for all target nuclei without a significant variation with the nuclear target mass are found. Also, no evidence for a dependence on the invariant mass W of the photon-nucleon system is found when the results are compared to those of previous experiments

Single Spin Asymmetries of Inclusive Hadrons Produced in Electron Scattering from a Transversely Polarized 3^3He Target

We report the first measurement of target single-spin asymmetries (A$_N$) in the inclusive hadron production reaction, $e~$+$~^3\text{He}^{\uparrow}\rightarrow h+X$, using a transversely polarized $^3$He target. The experiment was conducted at Jefferson Lab in Hall A using a 5.9-GeV electron beam. Three types of hadrons ($\pi^{\pm}$, $\text{K}^{\pm}$ and proton) were detected in the transverse hadron momentum range 0.54 $<p_T<$ 0.74 GeV/c. The range of $x_F$ for pions was -0.29 $<x_F<$ -0.23 and for kaons -0.25 $<x_F<$-0.18. The observed asymmetry strongly depends on the type of hadron. A positive asymmetry is observed for $\pi^+$ and $\text{K}^+$. A negative asymmetry is observed for $\pi^{-}$. The magnitudes of the asymmetries follow $|A^{\pi^-}| < |A^{\pi^+}| < |A^{K^+}|$. The K$^{-}$ and proton asymmetries are consistent with zero within the experimental uncertainties. The $\pi^{+}$ and $\pi^{-}$ asymmetries measured for the $^3$He target and extracted for neutrons are opposite in sign with a small increase observed as a function of $p_T$.Comment: Updated version, submitted to Phys. Rev.

Measurement of pretzelosity asymmetry of charged pion production in Semi-Inclusive Deep Inelastic Scattering on a polarized 3^3He target

An experiment to measure single-spin asymmetries in semi-inclusive production of charged pions in deep-inelastic scattering on a transversely polarized $^3$He target was performed at Jefferson Lab in the kinematic region of $0.16<x<0.35$ and $1.4<Q^2<2.7$ ${\rm GeV^2}$. The pretzelosity asymmetries on $^3$He, which can be expressed as the convolution of the $h^\perp_{1T}$ transverse momentum dependent distribution functions and the Collins fragmentation functions in the leading order, were measured for the first time. Using the effective polarization approximation, we extracted the corresponding neutron asymmetries from the measured $^3$He asymmetries and cross-section ratios between the proton and $^3$He. Our results show that for both $\pi^{\pm}$ on $^3$He and on the neutron the pretzelosity asymmetries are consistent with zero within experimental uncertainties.Comment: 6 pages, 3 figures; enlarged the legends in Fig.3; added 3 citation