110 research outputs found
<em>Polyamine Oxidase 5</em> loss-of-function mutations in <em>Arabidopsis thaliana</em> trigger metabolic and transcriptional reprogramming and promote salt stress tolerance
Problemas de corrosión
93 páginas.Compendio de problemas para el estudio de la corrosión, para estudiantes, técnicos e ingenieros entre otros, dedicados al estudio de esta área, visión de cómo resolver problemas que se puedan presentar en la vida diaria
Global Analyses Of Ceratocystis Cacaofunesta Mitochondria: From Genome To Proteome.
The ascomycete fungus Ceratocystis cacaofunesta is the causal agent of wilt disease in cacao, which results in significant economic losses in the affected producing areas. Despite the economic importance of the Ceratocystis complex of species, no genomic data are available for any of its members. Given that mitochondria play important roles in fungal virulence and the susceptibility/resistance of fungi to fungicides, we performed the first functional analysis of this organelle in Ceratocystis using integrated omics approaches. The C. cacaofunesta mitochondrial genome (mtDNA) consists of a single, 103,147-bp circular molecule, making this the second largest mtDNA among the Sordariomycetes. Bioinformatics analysis revealed the presence of 15 conserved genes and 37 intronic open reading frames in C. cacaofunesta mtDNA. Here, we predicted the mitochondrial proteome (mtProt) of C. cacaofunesta, which is comprised of 1,124 polypeptides - 52 proteins that are mitochondrially encoded and 1,072 that are nuclearly encoded. Transcriptome analysis revealed 33 probable novel genes. Comparisons among the Gene Ontology results of the predicted mtProt of C. cacaofunesta, Neurospora crassa and Saccharomyces cerevisiae revealed no significant differences. Moreover, C. cacaofunesta mitochondria were isolated, and the mtProt was subjected to mass spectrometric analysis. The experimental proteome validated 27% of the predicted mtProt. Our results confirmed the existence of 110 hypothetical proteins and 7 novel proteins of which 83 and 1, respectively, had putative mitochondrial localization. The present study provides the first partial genomic analysis of a species of the Ceratocystis genus and the first predicted mitochondrial protein inventory of a phytopathogenic fungus. In addition to the known mitochondrial role in pathogenicity, our results demonstrated that the global function analysis of this organelle is similar in pathogenic and non-pathogenic fungi, suggesting that its relevance in the lifestyle of these organisms should be based on a small number of specific proteins and/or with respect to differential gene regulation. In this regard, particular interest should be directed towards mitochondrial proteins with unknown function and the novel protein that might be specific to this species. Further functional characterization of these proteins could enhance our understanding of the role of mitochondria in phytopathogenicity.149
Enhanced transmission of malaria parasites to mosquitoes in a murine model of type 2 diabetes
Electrochemical Characterization of Modified Concretes with Sugar Cane Bagasse Ash
Corrosion is one of the most serious causes that reduce service life of Reinforced Concrete Structures (RCS). This is why it is necessary to create concrete mixtures that add durability for steel and that reduce impact on the environment. The use of agro-industrial waste materials rich in SiO2, Al2O3 and Fe2O3, added to concrete, has been the subject of research in recent years, because these pozzolanic materials improves o mecharacteristics of concrete, as mechanical strength, sulfate resistance and lower permeability. Binary Concretes were made and evaluated in the impact of Sugar Cane Bagasse Ash (SCBA) as a partial substitute for Portland cement, with the aim of reducing gtherate of corrosion induced by chloride ions and sulfate. The behavior of corrosion was monitored for 14 months in two aqueous solutions of NaCl and Na2SO4 both at 3.5%, using electrochemical techniques of corrosion potential (Ecorr) and linear polarization resistance (Rp). Under the conditions of study, the binary mixture that showed a better corrosion protection was the one that contained 80% from sugar Cane bagasse ash and 20% Portland cement
Corrosion Behaviour of 304 Austenitic, 15-5PH and 17-4PH Passive Stainless Steels in acid solutions
The objective of this work was to study is use electrochemical techniques to determinate the growth conditions, characteristics and resistance of passive layers of stainless steel (SS): 304 austenitic, 17-4PH (precipitation hardening) and 15-5PH. Passivation of the SS was performed in 15% citric acid at temperatures of 25 and 49 °C. The corrosion kinetics was obtained using the electrochemical technique as potentiodynamic polarization (PP), in a three-electrode system. The electrolytes used were sodium chloride (5 wt. % NaCl) and sulfuric acid (1 wt. % H2SO4). Passivation in citric acid allows obtain passive layers at temperatures of 49°C with immersion times of 30 minutes. In precipitation hardening steels, passive layers up to 360 mV in sodium chloride. Can be obtained. In sulfuric acid, there is a mechanism of passivation – transpassivation - secondary passivation, this due to the high electropositive values of potential
Global analyses of Ceratocystis cacaofunesta mitochondria: from genome to proteome
Abstract Background The ascomycete fungus Ceratocystis cacaofunesta is the causal agent of wilt disease in cacao, which results in significant economic losses in the affected producing areas. Despite the economic importance of the Ceratocystis complex of species, no genomic data are available for any of its members. Given that mitochondria play important roles in fungal virulence and the susceptibility/resistance of fungi to fungicides, we performed the first functional analysis of this organelle in Ceratocystis using integrated “omics” approaches. Results The C. cacaofunesta mitochondrial genome (mtDNA) consists of a single, 103,147-bp circular molecule, making this the second largest mtDNA among the Sordariomycetes. Bioinformatics analysis revealed the presence of 15 conserved genes and 37 intronic open reading frames in C. cacaofunesta mtDNA. Here, we predicted the mitochondrial proteome (mtProt) of C. cacaofunesta, which is comprised of 1,124 polypeptides - 52 proteins that are mitochondrially encoded and 1,072 that are nuclearly encoded. Transcriptome analysis revealed 33 probable novel genes. Comparisons among the Gene Ontology results of the predicted mtProt of C. cacaofunesta, Neurospora crassa and Saccharomyces cerevisiae revealed no significant differences. Moreover, C. cacaofunesta mitochondria were isolated, and the mtProt was subjected to mass spectrometric analysis. The experimental proteome validated 27% of the predicted mtProt. Our results confirmed the existence of 110 hypothetical proteins and 7 novel proteins of which 83 and 1, respectively, had putative mitochondrial localization. Conclusions The present study provides the first partial genomic analysis of a species of the Ceratocystis genus and the first predicted mitochondrial protein inventory of a phytopathogenic fungus. In addition to the known mitochondrial role in pathogenicity, our results demonstrated that the global function analysis of this organelle is similar in pathogenic and non-pathogenic fungi, suggesting that its relevance in the lifestyle of these organisms should be based on a small number of specific proteins and/or with respect to differential gene regulation. In this regard, particular interest should be directed towards mitochondrial proteins with unknown function and the novel protein that might be specific to this species. Further functional characterization of these proteins could enhance our understanding of the role of mitochondria in phytopathogenicity
The Influence of International Law on the International Movement of Persons
Many migration theories identify ‘the law’ as a significant constraint on the international
movement of persons. While this constraint often operates through national migration
legislation, this study examines the influence of international law in shaping contemporary
patterns in the international movement of persons at the macro level. The analysis begins with an
examination of the long-established power of a State to regulate cross-border movement of
persons as an inherent attribute of State sovereignty, together with the accepted limitations on a
State’s power to control entry and exit. Yet, international law reaches well beyond the movement
of people across borders. The development of international human rights law has been a key
constraint on state action in the United Nations era by also regulating the treatment of migrants
within a State’s borders. The study considers how international law has responded to current
migration issues, including: protection of migrant women and children; suppression of
smuggling and trafficking of people; labour migration; and environmental migration. As in other
areas of international society, there has been a proliferation of institutions through which
international migration law is made and enforced. The most prominent among them are the
United Nations High Commissioner for Refugees (UNHCR) and the International Organization
for Migration (IOM), but the establishment of other entities with overlapping mandates has given
rise to calls for a new international migration regime based on streamlined institutional
arrangements. The study concludes that international law is an imperfect framework for
regulating the international movement of persons because it has developed in a piecemeal
fashion over a long time to deal with issues of concern at particular points in human history. Yet,
despite its shortfalls, international law and its associated institutions unquestionably play a most
important role in constraining and channeling state authority over the international movement of
persons
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