316 research outputs found
Large deviations for clocks of self-similar processes
The Lamperti correspondence gives a prominent role to two random time
changes: the exponential functional of a L\'evy process drifting to
and its inverse, the clock of the corresponding positive self-similar process.
We describe here asymptotical properties of these clocks in large time,
extending the results of Yor and Zani
Gold/Silica biochips: applications to Surface Plasmon Resonance and fluorescence quenching
We report Gold/Silica biochips for low cost biosensor devices. Firstly, the
study of biochemical interactions on silica by means of Surface Plasmon
Resonance (SPR) is presented. Secondly, Gold/Silica biochips are employed to
reduce the strong quenching that occurs when a fluorophore is close to the gold
surface. Furthermore, the control of the Silica-like thickness allows
optimizing the distance between the metallic surface and the fluorophore in
order to enhance the fluorescent signal. These results represent the first
steps towards highly sensitive, specific and low cost biosensors based, for
example, on Surface Plasmon Coupled Emission (SPCE) techniques
High expression of gabarapl1 is associated with a better outcome for patients with lymph node-positive breast cancer
International audienceBACKGROUND: This study evaluates the relation of the early oestrogen-regulated gene gabarapl1 to cellular growth and its prognostic significance in breast adenocarcinoma. METHODS: First, the relation between GABARAPL1 expression and MCF-7 growth rate was analysed. Thereafter, by performing macroarray and reverse transcriptase quantitative-polymerase chain reaction (RT-qPCR) experiments, gabarapl1 expression was quantified in several histological breast tumour types and in a retrospective cohort of 265 breast cancers. RESULTS: GABARAPL1 overexpression inhibited MCF-7 growth rate and gabarapl1 expression was downregulated in breast tumours. Gabarapl1 mRNA levels were found to be significantly lower in tumours presenting a high histological grade, with a lymph node-positive (pN+) and oestrogen and/or progesterone receptor-negative status. In univariate analysis, high gabarapl1 levels were associated with a lower risk of metastasis in all patients (hazard ratio (HR) 4.96), as well as in pN+ patients (HR 14.96). In multivariate analysis, gabarapl1 expression remained significant in all patients (HR 3.63), as well as in pN+ patients (HR 5.65). In univariate or multivariate analysis, gabarapl1 expression did not disclose any difference in metastasis risk in lymph node-negative patients. CONCLUSIONS: Our data show for the first time that the level of gabarapl1 mRNA expression in breast tumours is a good indicator of the risk of recurrence, specifically in pN+ patients
Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila
Perception of temperature is an important brain function for organisms to survive. Evidence suggests that temperature preference behavior (TPB) in Drosophila melanogaster, one of poikilothermal animals, is regulated by cAMP-dependent protein kinase (PKA) signaling in mushroom bodies of the brain. However, downstream targets for the PKA signaling in this behavior have not been identified. From a genome-wide search for the genes regulated by PKA activity in the mushroom bodies, we identified the cyp6a17 Cytochrome P450 gene as a new target for PKA. Our detailed analysis of mutants by genetic, molecular and behavioral assays shows that cyp6a17 is essential for temperature preference behavior. cyp6a17 expression is enriched in the mushroom bodies of the adult brain. Tissue-specific knockdown and rescue experiments demonstrate that cyp6a17 is required in the mushroom bodies for normal temperature preference behavior. This is the first study, to our knowledge, to show PKA-dependent expression of a cytochrome P450 gene in the mushroom bodies and its role as a key factor for temperature preference behavior. Taken together, this study reveals a new PKA-Cytochrome P450 pathway that regulates the temperature preference behavior
Implication of sperm RNAs in transgenerational inheritance of the effects of early trauma in mice.
Small non-coding RNAs (sncRNAs) are potential vectors at the interface between genes and environment. We found that traumatic stress in early life altered mouse microRNA (miRNA) expression, and behavioral and metabolic responses in the progeny. Injection of sperm RNAs from traumatized males into fertilized wild-type oocytes reproduced the behavioral and metabolic alterations in the resulting offspring.We thank M. Rassoulzadegan and V. Grandjean for help with the sperm
purification, F. Manuella and H. Hörster for assistance with the MSUS paradigm,
H. Welzl for help with behavior, G. Vernaz for help with western blotting,
R. Tweedie-Cullen and P. Nanni for help with mass spectrometry, A. Patrignani
for advice on DNA and RNA quality assessment, and A. Chen and A. Brunner for
constructive discussions. This work was supported by the Austrian Academy of
Sciences, the University of ZĂŒrich, the Swiss Federal Institute of Technology,
Roche, the Swiss National Science Foundation, and The National Center of
Competence in Research âNeural Plasticity and Repairâ. P.S. was supported by a
Gonville and Caius College fellowship.This is the accepted manuscript. The final version is available in Nature Neuroscience 17, 667â669 (2014), doi:10.1038/nn.369
New early Triassic Lingulidae (Brachiopoda) genera and species from South China
Two new genera, Sinolingularia gen. nov. and Sinoglottidia gen. nov., together with three new species, Sinolingularia huananensis gen. et sp. nov., Sinolingularia yini gen. et sp. nov. and Sinoglottidia archboldi gen. et sp. nov., are described on the basis of a large collection of well-preserved specimens from several sections straddling the Permian - Triassic boundary in South China. <br /
Zika puzzle in Brazil: peculiar conditions of viral introduction and dissemination - A Review
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