Analysis of the Regulation and Deregulation of U.S. Ocean and Fis

While changes in political leadership affect U.S. ocean and coastal management strategies, the trajectory of U.S. policy over time has been to advance comprehensive consideration of the interconnected ecological ocean system in international and domestic ocean management. Domestically, regional planning and protective approaches have helped regulators balance multiple, often conflicting uses that can affect ecosystem resilience.However, U.S. wariness of multi-lateral international agreements challenges environmentally conscious ocean management goals. Recent domestic ocean policies emphasize fossil energy development over conservation and sustainability concerns. Proposals regarding offshore resource development as well as deregulatory efforts could impact ocean resources and have repercussions in international fora.At the domestic level, limits on the current administration's ability to abruptly finalize major changes to ocean and coastal management exist: jurisdictional authorities are split among federal and state powers and among multiple agencies, and science-based and procedural requirements are built into the ocean and coastal statutes. The current administration has shown a willingness to continue implementation of certain fisheries management reforms initiated in the prior administration, perhaps indicating certain policy areas may not experience extensive priority shifts.This paper reviews the legal and regulatory framework supporting U.S. coastal and ocean management, and describes changes under the current administration.a Comprehensive reviews of the legal framework and regulations of topics covered in this report already exist, but there is value in considering the overarching legal framework and understanding how these separate technical areas interrelate. This paper focuses on policy topics prioritized under the current and most recent administrations and assesses the state of play of the ongoing deregulation process.Overall, ocean management has seen less dramatic change than other areas of environmental regulation during this administration, such as air, water, climate, and energy. Most action on ocean issues has, thus far, concentrated on domestic policy. However, the themes exhibited at the domestic level are beginning to reflect on the international stage and to shift the dialogue with the EU and other partners.The administration's unwillingness to continue previous domestic policies on climate change and opposition to international agreements involving commitments to do so (for example, in announcing the U.S. intends to withdraw from the Paris Climate Agreement) have a direct impact on Arctic policies and may also impact ocean and coastal issues closely tied to climate, such as acidification, sea level rise, and adaptation. Yet, at the national level, this administration has supported narrow efforts to address marine debris and plastics in the oceans.Policies and actions in areas crucial to the management of ocean and coastal areas reveal a pattern of prioritizing economic interests and energy development over conservation and protection. On issues not directly tied to climate and not thought to hinder U.S. energy industry development, this administration has exhibited a degree of continuity in position with the prior administration—such as on illegal, unreported, and unregulated (IUU) fishing and signing of an agreement preemptively barring fishing in the Central Arctic Ocean. Continued development of offshore wind energy could also be an avenue for increased cooperation with the EU as much of the existing expertise in this area lies with EU-based companies. Recent estimates suggest there are 22,000 MW of offshore wind potential off the east coast of the U.S.—representing a possible $70 billion of economic opportunity

Metabolic and transcriptomic analysis of Huntington's disease model reveal changes in intracellular glucose levels and related genes.

Huntington's Disease (HD) is a neurodegenerative disorder caused by an expansion in a CAG-tri-nucleotide repeat that introduces a poly-glutamine stretch into the huntingtin protein (mHTT). Mutant huntingtin (mHTT) has been associated with several phenotypes including mood disorders and depression. Additionally, HD patients are known to be more susceptible to type II diabetes mellitus (T2DM), and HD mice model develops diabetes. However, the mechanism and pathways that link Huntington's disease and diabetes have not been well established. Understanding the underlying mechanisms can reveal potential targets for drug development in HD. In this study, we investigated the transcriptome of mHTT cell populations alongside intracellular glucose measurements using a functionalized nanopipette. Several genes related to glucose uptake and glucose homeostasis are affected. We observed changes in intracellular glucose concentrations and identified altered transcript levels of certain genes including Sorcs1, Hh-II and Vldlr. Our data suggest that these can be used as markers for HD progression. Sorcs1 may not only have a role in glucose metabolism and trafficking but also in glutamatergic pathways affecting trafficking of synaptic components

Influenza Cases From Surveillance Acute Respiratory Infection in Indonesia, 2011

Background: Severe Acute Respiratory Infection (SARI) or pneumonia has a major contribution in the morbidity and mortality, however, Indonesia still has lack of its epidemiology. The aim of the study is to know the epidemiological pattern of influenza virus as the etiology of SARI cases. Methods:This analysis based on the sentinel surveillanceSARI conducted by Center for Biomedical and Basic Technology of Health (CBBTH) of Indonesia carried out at nine hospitals in nine provinces in 2011.Every patient who met the criteria of SARI was included in this study. Serum, throat and nasal swabs were taken and examined at the Virology laboratory CBBTH to determine the etiology. RT-PCR was used to detect type and subtype of influenza viruses from swabs.Results: Total number of SARI cases were 333. We found 6% cases were influenza positive by RT-PCR. The proportion of influenza A was 5% and influenza B 1% from total SARI cases. We detected that seasonal influenza A subtype H1N1pdm09was the dominant subtypes that circulating in Indonesia.Conclusion: We foundseasonal Influenza infection from SARI patients, however, it was only small number. Therefore, further detection of SARI cases is needed. (Health Science Indones 2014;1:7-11

Deteksi Resistensi Oseltamivir Influenza a (H1N1pdm09) Dari Pasien Infeksi Saluran Pernafasan Akut Berat Di Indonesia Tahun 2014

Influenza viruses are classified into subtypes based on two surface antigens known as hemagglutinin (HA) and neuraminidase (NA). Antigenic changes of influenza virus can cause resistance to antiviral drugs which is already limited in variation. Resistance to the drugs used recently are due to antigenic drift by point mutation in a single amino acid at position 275 (H275Y). The purpose of this research is to identify the presence of influenza virus A (H1N1pdm09) that were resistant to oseltamivir from cases of severe acute respiratory infection (SARI)in Indonesia in 2014 by using a rapid detection test. Detection of oseltamivir resistance in NA gene is to identify the single nucleotide polimorphism (SNP) at position 275 (H275Y) using the real-time RT-PCR method from clinical specimens SARI case. A total of 870 specimens from six sentinel hospitals were collected and 15 of them positive H1N1pdm09. Of the 15 clinical specimens, H1N1pdm09 virus strains that have mutations H275Y were not found. Based on this finding, it can be concluded that during the year 2014, there is no influenza virus A (H1N1pdm09) resistant to oseltamivir from SARI cases specimen in six sentinel hospitals in Indonesia

A triazine-based BODIPY trimer as a molecular viscometer

Photophysical behaviour of a novel trimeric BODIPY rotor with a high extinction coefficient is reported. Steady state and time resolved fluorescence measurements established that the trimer could be used as a viscometer for molecular solvents, membrane-like environments and several cancer cell lines

Mutation bias shapes the spectrum of adaptive substitutions

Evolutionary adaptation often occurs by the fixation of beneficial mutations. This mode of adaptation can be characterized quantitatively by a spectrum of adaptive substitutions, i.e., a distribution for types of changes fixed in adaptation. Recent work establishes that the changes involved in adaptation reflect common types of mutations, raising the question of how strongly the mutation spectrum shapes the spectrum of adaptive substitutions. We address this question with a codon-based model for the spectrum of adaptive amino acid substitutions, applied to three large datasets covering thousands of amino acid changes identified in natural and experimental adaptation in Saccharomyces cerevisiae, Escherichia coli, and Mycobacterium tuberculosis Using species-specific mutation spectra based on prior knowledge, we find that the mutation spectrum has a proportional influence on the spectrum of adaptive substitutions in all three species. Indeed, we find that by inferring the mutation rates that best explain the spectrum of adaptive substitutions, we can accurately recover the species-specific mutation spectra. However, we also find that the predictive power of the model differs substantially between the three species. To better understand these differences, we use population simulations to explore the factors that influence how closely the spectrum of adaptive substitutions mirrors the mutation spectrum. The results show that the influence of the mutation spectrum decreases with increasing mutational supply ([Formula: see text]) and that predictive power is strongly affected by the number and diversity of beneficial mutations

Expression of sugarcane genes induced by inoculation with Gluconacetobacter diazotrophicus and Herbaspirillum rubrisubalbicans

Several Brazilian sugarcane varieties have the ability to grow with little addition of inorganic nitrogen fertilizers, showing high contributions of Biological Nitrogen Fixation (BNF). A particular type of nitrogen-fixing association has been described in this crop, where endophytic diazotrophs such as Gluconacetobacter diazotrophicus and Herbaspirillum spp. colonize plant tissues without causing disease symptoms. In order to gain insight into the role played by the sugarcane in the interaction between this plant and endophytic diazotrophs, we investigated gene expression profiles of sugarcane plants colonized by G. diazotrophicus and H. rubrisubalbicans by searching the sugarcane expressed sequence tag SUCEST Database (http://sucest.lad.ic.unicamp.br/en/). We produced an inventory of sugarcane genes, candidates for exclusive or preferential expression during the nitrogen-fixing association. This data suggests that the host plant might be actively involved in the establishment of the interaction with G. diazotrophicus and H. rubrisubalbicans.Diversos genótipos brasileiros de cana-de-açúcar são capazes de crescer com baixa adição de adubos nitrogenados, obtendo elevadas contribuições da Fixação Biológica de Nitrogênio (FBN). Um tipo especial de associação com bactérias fixadoras de nitrogênio foi descrito em cana-de-açúcar, onde as bactérias endofíticas, como Gluconacetobacter diazotrophicus e Herbaspirillum spp., colonizam o interior dos tecidos vegetais, sem causar sintomas de doença. Com o objetivo de tentar entender o papel da cana-de-açúcar nesse tipo de associação, nós investigamos os perfis de expressão gênica de plantas colonizadas pelos diazotróficos endofíticos, usando o banco de dados do SUCEST. Um catálogo com os genes de cana-de-açúcar que são candidatos a se expressar exclusivamente ou preferencialmente durante a associação foi gerado. Esses dados preliminares sugerem que a cana-de-açúcar deve ter uma participação ativa na interação, respondendo a diversos processos metabólicos durante a associação.199206Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES