Studii preliminare privind cultura plantelor legumicole în ghivece şi containere

This paper presents a literature review of the vegetable growing in pots and containers. Growing vegetables in this system it is known for a long time in the countries of Western Europe and in some areas of our country. Adopting this system in Romania requires in-depth studies regarding: the suitable species, the type of pots and soil recipes needed, crop establishment and maintenance (fertilizers aplication, irrigation) and, in some cases, optimization of the certain referring to light and placement of pots and containers. This cultivation system is spread mainly in peri-urban areas were the interest among gardeners to grow their own crops and secure their vegetable needs is high and has a favorable environment. Vegetable plants cultivated in pots and containers present a large diversity worldwide but, within this diversity, the climatic conditions from our country must be appropriate for an efficient activity

Cercetari privind comportarea unor specii legumicole în ghivece şi containere

The paper presents the research results regarding the evaluation of morphological, physiological and production characteristics in some vegetable plants grown in pots and containers. The vegetable plants studiedwere: cherry tomatoes, sweet peppers, climbing beans, dwarf beans, oregano and lovage, and for pot culture: hot peppers, basil, salad, thyme, dill and parsley. Remarkable results were obtained for the following vegetable species: cherry tomatoes 1656.0 g / pl ant andpepper 2305g / pl for container culture, and for pot culture the results were: hot pepper, 351.3g / pl and salad 250.0 g / pl

Studii preliminare privind utilizarea speciilor legumicole în conceptul grădinilor urbane

This paper presents a literature preview regarding the use of vegetable species in the concept of urban gardens. The designing of the utilitarian gardens dates back from the antiquity when they were particularly important as a source of food. With the emergence of the concept of "edible landscaping", which promotes the use of edible plant species along with ornamental plant species in landscaping, the utilitarian garden has acquired aesthetic valences as an integral part of the green space set up on private property in the urban area and not only. Starting from the desire of people to have a place to produce some of the necessary vegetables and aromatic plants in the small space around the houses and to enjoy a recreation space, will be studied different systems of use of vegetable plants in order to develop concrete measures for the development of decorative vegetable gardens in the private environment in urban and periurban areas, taking into account the possibilities of association of the leguminous plants in raised bed

Rezultate partiale privind tipurile de substraturi folosite pentru obţinerea culturilor legumicole de tip microgreens

The paper addresses research on the use of four types of substrates (coconut substrate, 80% peat + 20% coconut waste, 80% peat + 20% sand and80% peat + 20% pearlite) to obtain the products microgreens type vegetables, in order to establish the best substrate variant. The best results were obtained on the coconut waste substrate

Studii preliminare privind alegerea şi pregătirea substraturilor pentru cultura la ghivece şi container

The paper presents a study on the main pedological, agrochemical and biological indices of the different types of substrate used for growing vegetables in pots and containers in order to optimize the growth and development of plants. The researchewere carried out in an experimental field set up at the Didactic Center of the University of Agricultural Sciences and Veterinary Medicine Iasi. Three types of substrate were used: S1 -substrate made from 35% garden soil, 35% compost, 20% peat, 10% sandand pearlite, S2-substrate made of earth 35% garden, 20% compost, 35% peat, 10% sand and pearlite; S3 -commercially available substrate. Determinations havebeen made on:nutrients, pH, humidity and granulometry. Substrate quality assessment was performed on the basis of yield and harvest qualit

Grădini legumicole ornamentale in sistem familial

Ornamental vegetable gardens have a long history on the European continent. The design of the gardens is different due to influences originated from customs and traditions, which represent important elements in their composition and are reflected in the vegetable growing methods and species used. The purpose of this paper is to analyse the opportunities that these gardens have and to satisfy the nutritional and aesthetic needs of a family.To reach the aim and proposed objectives a series of experiments and case studies were conducted. By combining the owners underlined nutritional needs from our previous studies and the obtained results from our experiments, applicable solutions were created for family vegetable gardens.From a therapeutic pointof view this type of landscape design helps maintain cultural identity, encouraging communication and socialization between members of a community.The obtained results show that the studied family gardens situated in urban areas have a positive influenceon the sustainability of the community maintaining a “heathy life style” for its inhabitants

HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth

The reduced diameter of skeletal myofibres is a hallmark of several congenital myopathies, yet the underlying cellular and molecular mechanisms remain elusive. In this study, we investigate the role of HACD1/PTPLA, which is involved in the elongation of the very long chain fatty acids, in muscle fibre formation. In humans and dogs, HACD1 deficiency leads to a congenital myopathy with fibre size disproportion associated with a generalized muscle weakness. Through analysis of HACD1-deficient Labradors, Hacd1-knockout mice, and Hacd1-deficient myoblasts, we provide evidence that HACD1 promotes myoblast fusion during muscle development and regeneration. We further demonstrate that in normal differentiating myoblasts, expression of the catalytically active HACD1 isoform, which is encoded by a muscle-enriched splice variant, yields decreased lysophosphatidylcholine content, a potent inhibitor of myoblast fusion, and increased concentrations of ≥C18 and monounsaturated fatty acids of phospholipids. These lipid modifications correlate with a reduction in plasma membrane rigidity. In conclusion, we propose that fusion impairment constitutes a novel, non-exclusive pathological mechanism operating in congenital myopathies and reveal that HACD1 is a key regulator of a lipid-dependent muscle fibre growth mechanism

Cap-Gly Proteins at Microtubule Plus Ends: Is EB1 Detyrosination Involved?

Localization of CAP-Gly proteins such as CLIP170 at microtubule+ends results from their dual interaction with α-tubulin and EB1 through their C-terminal amino acids −EEY. Detyrosination (cleavage of the terminal tyrosine) of α-tubulin by tubulin-carboxypeptidase abolishes CLIP170 binding. Can detyrosination affect EB1 and thus regulate the presence of CLIP170 at microtubule+ends as well? We developed specific antibodies to discriminate tyrosinated vs detyrosinated forms of EB1 and detected only tyrosinated EB1 in fibroblasts, astrocytes, and total brain tissue. Over-expressed EB1 was not detyrosinated in cells and chimeric EB1 with the eight C-terminal amino acids of α-tubulin was only barely detyrosinated. Our results indicate that detyrosination regulates CLIPs interaction with α-tubulin, but not with EB1. They highlight the specificity of carboxypeptidase toward tubulin

Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture

BACKGROUND: Stromal fibroblasts associated with in situ and invasive breast carcinoma differ phenotypically from fibroblasts associated with normal breast epithelium, and these alterations in carcinoma-associated fibroblasts (CAF) may promote breast carcinogenesis and cancer progression. A better understanding of the changes that occur in fibroblasts during carcinogenesis and their influence on epithelial cell growth and behavior could lead to novel strategies for the prevention and treatment of breast cancer. To this end, the effect of CAF and normal breast-associated fibroblasts (NAF) on the growth of epithelial cells representative of pre-neoplastic breast disease was assessed. METHODS: NAF and CAF were grown with the nontumorigenic MCF10A epithelial cells and their more transformed, tumorigenic derivative, MCF10AT cells, in direct three-dimensional co-cultures on basement membrane material. The proliferation and apoptosis of MCF10A cells and MCF10AT cells were assessed by 5-bromo-2'-deoxyuridine labeling and TUNEL assay, respectively. Additionally, NAF and CAF were compared for expression of insulin-like growth factor II as a potential mediator of their effects on epithelial cell growth, by ELISA and by quantitative, real-time PCR. RESULTS: In relatively low numbers, both NAF and CAF suppressed proliferation of MCF10A cells. However, only NAF and not CAF significantly inhibited proliferation of the more transformed MCF10AT cells. The degree of growth inhibition varied among NAF or CAF from different individuals. In greater numbers, NAF and CAF have less inhibitory effect on epithelial cell growth. The rate of epithelial cell apoptosis was not affected by NAF or CAF. Mean insulin-like growth factor II levels were not significantly different in NAF versus CAF and did not correlate with the fibroblast effect on epithelial cell proliferation. CONCLUSION: Both NAF and CAF have the ability to inhibit the growth of pre-cancerous breast epithelial cells. NAF have greater inhibitory capacity than CAF, suggesting that the ability of fibroblasts to inhibit epithelial cell proliferation is lost during breast carcinogenesis. Furthermore, as the degree of transformation of the epithelial cells increased they became resistant to the growth-inhibitory effects of CAF. Insulin-like growth factor II could not be implicated as a contributor to this differential effect of NAF and CAF on epithelial cell growth