RF spectroscopy in a resonant RF-dressed trap

We study the spectroscopy of atoms dressed by a resonant radiofrequency (RF) field inside an inhomogeneous magnetic field and confined in the resulting adiabatic potential. The spectroscopic probe is a second, weak, RF field. The observed line shape is related to the temperature of the trapped cloud. We demonstrate evaporative cooling of the RF-dressed atoms by sweeping the frequency of the second RF field around the Rabi frequency of the dressing field.Comment: 7 figures, 8 pages; to appear in J. Phys.

Alterations of immune response of non-small lung cancer with azacytidine

Innovative therapies are needed for advanced Non-Small Cell Lung Cancer (NSCLC). We have undertaken a genomics based, hypothesis driving, approach to query an emerging potential that epigenetic therapy may sensitize to immune checkpoint therapy targeting PD-L1/PD-1 interaction. NSCLC cell lines were treated with the DNA hypomethylating agent azacytidine (AZA - Vidaza) and genes and pathways altered were mapped by genome-wide expression and DNA methylation analyses. AZA-induced pathways were analyzed in The Cancer Genome Atlas (TCGA) project by mapping the derived gene signatures in hundreds of lung adeno (LUAD) and squamous cell carcinoma (LUSC) samples. AZA up-regulates genes and pathways related to both innate and adaptive immunity and genes related to immune evasion in a several NSCLC lines. DNA hypermethylation and low expression of IRF7, an interferon transcription factor, tracks with this signature particularly in LUSC. In concert with these events, AZA up-regulates PD-L1 transcripts and protein, a key ligand-mediator of immune tolerance. Analysis of TCGA samples demonstrates that a significant proportion of primary NSCLC have low expression of AZA-induced immune genes, including PD-L1. We hypothesize that epigenetic therapy combined with blockade of immune checkpoints - in particular the PD-1/PD-L1 pathway - may augment response of NSCLC by shifting the balance between immune activation and immune inhibition, particularly in a subset of NSCLC with low expression of these pathways. Our studies define a biomarker strategy for response in a recently initiated trial to examine the potential of epigenetic therapy to sensitize patients with NSCLC to PD-1 immune checkpoint blockade

Cauchy's infinitesimals, his sum theorem, and foundational paradigms

Cauchy's sum theorem is a prototype of what is today a basic result on the convergence of a series of functions in undergraduate analysis. We seek to interpret Cauchy's proof, and discuss the related epistemological questions involved in comparing distinct interpretive paradigms. Cauchy's proof is often interpreted in the modern framework of a Weierstrassian paradigm. We analyze Cauchy's proof closely and show that it finds closer proxies in a different modern framework. Keywords: Cauchy's infinitesimal; sum theorem; quantifier alternation; uniform convergence; foundational paradigms.Comment: 42 pages; to appear in Foundations of Scienc

Managing Industrial Communication Delays with Software-Defined Networking

Recent technological advances have fostered the development of complex industrial cyber-physical systems which demand real-time communication with delay guarantees. The consequences of delay requirement violation in such systems may become increasingly severe. In this paper, we propose a contract-based fault-resilient methodology which aims at managing the communication delays of real-time flows in industries. With this objective, we present a light-weight mechanism to estimate end-to-end delay in the network in which the clocks of the switches are not synchronized. The mechanism aims at providing high level of accuracy with lower communication overhead. We then propose a contract-based framework using software-defined networking where the components are associated with delay contracts and a resilience manager. The proposed resilience management framework contains: (1) contracts which state guarantees about components behaviors, (2) observers which are responsible to detect contract failure (fault), (3) monitors to detect events such as run-time changes in the delay requirements and link failure, (4) control logic to take suitable decisions based on the type of the fault, (5) resilience manager to decide response strategies containing the best course of action as per the control logic decision. Finally, we present a delay-aware path finding algorithm which is used to route/reroute the real-time flows to provide resiliency in the case of faults and, to adapt to the changes in the network state. Performance of the proposed framework is evaluated with the Ryu SDN controller and Mininet network emulator

Sustainability of yield and value in blackgram (Vigna Mungo) through frontline demonstrations

The present study evaluated the sustainability of yield and value in blackgram cultivation by adopting improved production technologies through frontline demonstrations. These were implemented as sole and rice fallow crop cultivation in Tamil Nadu, India, to reduce yield gaps at farmers' fields. The scientific study was conducted in 2020-2021 with 295 demonstrations (118 hectares) using cluster village methodology to examine the effects of extension gap, technology gap and technology index on yield and economics by demonstrating improved technologies. These include mechanized sowing, use of disease-resistant varieties, integrated pest and weed management, seed treatment, crop nutrient boosters and mechanized harvesting. Yield enhancement of 51.55 % was recorded in a demonstrated package of technologies over farmers' practices, primarily due to the adoption of Yellow Mosaic Virus-resistant varieties, effective weed management strategies, and foliar sprays like TNAU pulse wonder to boost crop growth. The average extension gap (2.89 q/ha), technology gap (0.87 q/ha) and technology index of demonstrations (11.01 %) were documented, which are mainly due to efforts of multidisciplinary scientists’ field visits and farm advisories. A study reported higher weed control efficiency (48–76 %) in demo plots, enhancing demon yield than in control plots. Compared to farmers' plots (Rs. 37075/ha), average net returns in demonstrations were higher at Rs. 66732/ha. The study also disclosed an additional net return (Rs.29657/hectare) with an incremental BCR of 19.95, indicating a significant increase in profitability relative to the investment. The consistently greater Sustainability Yield Index (0.61 to 0.99) and Sustainability Value Index (0.88 to 0.93) in demo plots highlight improved stability and economic value of yields compared to farmer's plots. The latest package of technologies in blackgram paved the way for sustained yield under normal conditions, which are defined as fields with adequate moisture and timely sowing and rice fallow conditions. These advancements improved farmers’ livelihoods by increasing net returns and ensuring more stable and predictable yields

Characteristic Male Urine Microbiomes Associate with Asymptomatic Sexually Transmitted Infection

Article discussing research on characteristic male urine microbiomes associated with asymptomatic sexually transmitted infection

Knowledge, Attitude, and Practice Towards Responsible Self-Medication Among Pharmacy Students: A Web-Based Cross-Sectional Survey in Uganda

Bukoli Brian,1 Narayana Goruntla,1 Bhavana Reddy Bommireddy,2 Bhavani M Mopuri,2 Vigneshwaran Easwaran,3 Mohammad Jaffar Sadiq Mantargi,4 Durga Prasad Thammisetty,5 Sarad Pawar Naik Bukke,6 Tadele Mekuriya Yadesa,1 Ebere Emilia Ayogu7 1Department of Clinical Pharmacy and Pharmacy Practice, School of Pharmacy, Kampala International University, Western Campus, Ishaka, Uganda; 2Research Scholar, Department of Pharmacy Practice, Raghavendra Institute of Pharmaceutical Education and Research (RIPER) – Autonomous, Anantapur, AP, India; 3Department of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha, Kingdom of Saudi Arabia; 4Department of Pharmacology, Pharmacy Program, Batterjee Medical College, Jeddah, Saudi Arabia; 5Department of Pharmacy Practice, Sri Padmavathi School of Pharmacy, Tiruchanoor, Tirupathi, AP, India; 6Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, Kampala International University, Western Campus, Ishaka, Uganda; 7Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, NigeriaCorrespondence: Narayana Goruntla; Bukoli Brian, Department of Clinical Pharmacy and Pharmacy Practice, School of Pharmacy, Kampala International University, Western Campus, Ishaka, Uganda, Tel +256760060453, Email [email protected]; [email protected]; [email protected]: Rational self-medication (SM) practice among healthcare students is essential to promote the safe, effective, and economical use of medicines for self-diagnosed conditions. The study aimed to assess pharmacy students’ knowledge, attitude, and practice about responsible self-medication.Methodology: A cross-sectional online survey was conducted among Ugandan pharmacy students for one month from March 1 to March 31, 2024. The study included students enrolled in diploma, bachelor, and master of pharmacy programs in Uganda. Informed consent was obtained online by asking a question regarding willingness to participate. A non-probable snowball sampling technique was used to recruit students. A pre-validated questionnaire was used to obtain socio-demographics, self-medication practices, knowledge, attitudes, and practices about responsible self-medication. We used binary and multivariable logistic regression analysis to identify the factors associated with KAP regarding responsible self-medication.Results: The prevalence of self-medication practice among pharmacy students was 96.73%. The self-medication is most common in headache (78.97%), cold, and cough (79.91%) illnesses. Painkillers (90.19%) and antibiotics (53.97%) are the most common medicines used for self-medication. Most pharmacy students have good knowledge (87.38%) and a positive attitude (96.03%) toward responsible self-medication. However, only 27.34% of students practice rational self-medication. Participants’ university and parents’ professions were significantly associated with good knowledge of responsible self-medication. Whereas factors such as gender, residence, and type of illness were significantly associated with rational self-medication practice.Conclusion: The prevalence of self-medication was high among pharmacy students in Uganda. Painkillers and antibiotics are the most preferred drugs for self-medication. About 87.38% of pharmacy students have good knowledge, and 96.03% have a positive attitude toward responsible self-medication. Rational self-medication practice was low among students. We recommended training sessions to enhance safe self-medication practices among pharmacy students.Keywords: attitude, knowledge, pharmacy students, practices, self-medication, Ugand

Stromal Cell-Derived Factor-1/CXCL12 Contributes to MMTV-Wnt1 Tumor Growth Involving Gr1+CD11b+ Cells

BACKGROUND: Histological examinations of MMTV-Wnt1 tumors reveal drastic differences in the tumor vasculature when compared to MMTV-Her2 tumors. However, these differences have not been formally described, nor have any angiogenic factors been implicated to be involved in the Wnt1 tumors. METHODOLOGY/PRINCIPAL FINDINGS: Here, we show that MMTV-Wnt1 tumors were more vascularized than MMTV-Her2 tumors, and this correlated with significantly higher expression of a CXC chemokine, stromal cell-derived factor-1 (SDF1/CXCL12) but not with VEGFA. Isolation of various cell types from Wnt1 tumors revealed that SDF1 was produced by both tumor myoepithelial cells and stromal cells, whereas Her2 tumors lacked myoepithelial cells and contained significantly less stroma. The growth of Wnt1 tumors, but not Her2 tumors, was inhibited by a neutralizing antibody to SDF1, but not by neutralization of VEGFA. Anti-SDF1 treatment decreased the proportion of infiltrating Gr1(+) myeloid cells in the Wnt1 tumors, which correlated with a decrease in the percentage of endothelial cells. The involvement of Gr1(+) cells was evident from the retardation of Wnt1 tumor growth following in vivo depletion of these cells with an anti-Gr1-specific antibody. This degree of inhibition on Wnt1 tumor growth was comparable, but not additive, to the effect observed with anti-SDF1, indicative of overlapping mechanisms of inhibition. In contrast, Her2 tumors were not affected by the depletion of Gr1(+) cells. CONCLUSIONS/SIGNIFICANCE: We demonstrated that SDF1 is important for Wnt1, but not for HER2, in inducing murine mammary tumor and the role of SDF1 in tumorigenesis involves Gr1(+) myeloid cells to facilitate growth and/or angiogenesis

Nuclear β-catenin expression is closely related to ulcerative growth of colorectal carcinoma

Although most colorectal cancer develops based on the adenoma–adenocarcinoma sequence, morphologically, colorectal cancer is not a homogeneous disease entity. Generally, there are two distinct morphological types: polypoid and ulcerative colorectal tumours. Previous studies have demonstrated that K-ras codon 12 mutations are preferentially associated with polypoid growth of colorectal cancer; however, little is known about the molecular mechanism that determines ulcerative growth of colorectal cancer. β-catenin complex plays a critical role both in tumorigenesis and morphogenesis. We examined the differential expression of β-catenin and its related factors among different types of colorectal cancer in order to determine any relationship with gross tumour morphology. Immunohistochemical staining of β-catenin, E-cadherin and MMP-7 was performed on 51 tumours, including 26 polypoid tumours and 25 ulcerative tumours. Protein truncation tests and single-strand conformational polymorphism for mutation of the adenomatous polyposis coli tumour suppressor gene, as well as single-strand conformational polymorphism for the mutation of β-catenin exon 3 were also done. Nuclear expression of β-catenin was observed in 18 out of 25 (72%) cases of ulcerative colorectal cancer and seven out of 26 (26.9%) cases of polypoid colorectal cancer. A significant relationship of nuclear β-catenin expression with ulcerative colorectal cancer was found (P<0.001). However, this finding was independent of adenomatous polyposis coli tumour suppressor gene mutation and E-cadherin expression. Together with previous data, we propose that different combinations of genetic alterations may underlie different morphological types of colorectal cancer. These findings should be taken into consideration whenever developing a new genetic diagnosis or therapy for colorectal cancer

Post-Transcriptional Regulation of Cadherin-11 Expression by GSK-3 and β-Catenin in Prostate and Breast Cancer Cells

The cell-cell adhesion molecule cadherin-11 is important in embryogenesis and bone morphogenesis, invasion of cancer cells, lymphangiogenesis, homing of cancer cells to bone, and rheumatoid arthritis. However, very little is known about the regulation of cadherin-11 expression.Here we show that cell density and GSK-3beta regulate cadherin-11 levels in cancer cells. Inactivation of GSK3beta with lithium chloride or the GSK3 inhibitor BIO and GSK3beta knockdown with siRNA repressed cadherin-11 mRNA and protein levels. RNA Polymerase II chromatin immunoprecipitation experiments showed that inhibition of GSK3 does not affect cadherin-11 gene transcription. Although the cadherin-11 3'UTR contains putative microRNA target sites and is regulated by Dicer, its stability is not regulated by GSK3 inhibition or density. Our data show that GSK3beta regulates cadherin-11 expression in two ways: first a beta-catenin-independent regulation of cadherin-11 steady state mRNA levels, and second a beta-catenin-dependent effect on cadherin-11 3'UTR stability and protein translation.Cadherin-11 mRNA and protein levels are regulated by the activity of GSK3beta and a significant degree of this regulation is exerted by the GSK3 target, beta-catenin, at the level of the cadherin-11 3'UTR