Processing and size range separation of pristine and magnetic poly(L-lactic acid) based microspheres for biomedical applications

"Available online 11 May 2016"Biodegradable poly(L-lactic acid) (PLLA) and PLLA/CoFe2O4 magnetic microspheres with average sizes ranging between 0.16 -3.9 m and 0.8-2.2 m, respectively, were obtained by an oil-in-water emulsion method using poly(vinyl alcohol) (PVA) solution as the emulsifier agent. The separation of the microspheres in different size ranges was then performed by centrifugation and the colloidal stability assessed at different pH values. Neat PLLA spheres are more stable in alkaline environments when compared to magnetic microspheres, both types being stable for pHs higher than 4, resulting in a colloidal suspension. On the other hand, in acidic environments the microspheres tend to form aggregates. The neat PLLA microspheres show a degree of crystallinity of 40% whereas the composite ones are nearly amorphous (17%). Finally, the biocompatibility was assessed by cell viability studies with MC3T3-E1 pre-osteoblast cells.This work is funded by FCT – Fundação para a Ciência e a Tecnologia, grants SFRH/BD/82411/2011 (DMC), SFRH/ BPD/90870/2012 (CR), SFRH/BD/98616/2013 (PMM) and SFRH/ BPD/97739/2013 (PM), respectively. The authors thank support from the COST Action MP1206 ‘‘Electrospun Nano-fibres for bio inspired composite materials and innovative industrial applications” and from the Basque Government Industry Department under the ELKARTEK Program. SLM thanks the Diputación Foral de Bizkaia for financial support under the Bizkaia Talent Programme; European Union’s Seventh Framework Programme; Marie Curie Actions–People; Grant agreement no. 267230

Monitorização da qualidade do leite de cabra durante a pasteurização e processamento óhmico usando sensores de fibra óptica

Este estudo avalia a viabilidade da espectroscopia UVVIS- SWNIR na degradação da qualidade do leite de cabra durante a pasteurização e aquecimento Ohmico. Foram utilizados: i) leite de cabra cru; ii) leite não processado, mas passado pelas bombas do pasteurizador e aquecimento Ohmico; e iii) leite processado por pasteurização e aquecimento Ohmico. Os espectros são recolhidos por sensor de fibra óptica em comprimentos de onda UVVIS e UV-SWNIR. As amostras foram realizadas a uma temperatura de (18±2.0ºC), e os dados foram registados em cada produto e para cada gama espectral. Os espectros das amostras foram então normalizados e corrigidos usando o algoritmo de correcção da robusta da dispersão de radiação, sendo depois decomposto utilizando o algoritmo de decomposição de valores singulares relevantes. Os resultados demonstram que a espectroscopia de refletância dá-nos uma avaliação rápida das características do leite de cabra em vários cenários de produção, sendo um execelente sensor para a monitorização e automatização do processamento de lacticínios, em especial para o desenvolvimento da monitorização e controlo com a utilização de software de Tecnometria

Influence of ferrite nanoparticle type and content on the crystallization kinetics and electroactive phase nucleation of poly(vinilidene fluoride)

This work reports on the nucleation of the β-phase of poly(vinylidene fluoride), PVDF, by incorporating CoFe2O4 and NiFe2O4 nanoparticles, leading in this way, to the preparation of magnetoelectric composites. The fraction of filler nanoparticles needed to produce the same β- to α-phase ratio in crystallized PVDF is one order of magnitude lower in the Cobalt nanoparticles. The interaction between nanoparticles and PVDF chains induce the all trans conformation in PVDF segments and this structure then propagates in crystal growth. The nucleation kinetics is enhanced by the presence of nanoparticles, as corroborated by the increasing number of spherulites with increasing nanoparticle content and by the variations of the Avrami’s exponent. Further, the decrease of the crystalline fraction of PVDF with increasing nanoparticles content indicates that an important fraction of polymer chains are confined in interphases with the filler particle.Centro de Investigación Principe FelipeInstituto de Salud Carlos III (Ministry of Science and Innovation).FEDER funds through the "Programa Operacional Factores de Competitividade – COMPETE"Fundação para a Ciência e a Tecnologia (FCT) - NANO/NMed-SD/0156/2007, PTDC/CTM/69316/2006, SFRH/BPD/63148/2009Conselleria de Sanidad (Generalitat Valenciana)Spanish Ministry of Education through project No. MAT2010-21611-C03-01, EUI2008-0012

Sliding mode control of an innovative single-switch three-level active rectifier

This paper presents the sliding mode control (SMC) applied to an innovative active rectifier. This proposed active rectifier is constituted by a single-switch, and operates with three voltage levels, evidencing a set of advantages when compared with conventional approaches of power factor correction topologies. Taking into account the diversity of applications for this type of power converter, the SMC is used in order to obtain a robust current tracking. Since the active rectifier is controlled according to the ac grid-side current, the error between such current and its reference is determined, and by employing the SMC, this error is minimized during each sampling period with the objective of selecting the state of the single-switch. A comprehensive description about the SMC implementation, supported by the overall operation of the active rectifier, is presented throughout the paper. The obtained computational results for a set of different operating conditions, comprising significant power ranges and sudden variations, confirm the accurate application of the SMC when applied to the proposed single-switch three-level active rectifier. A comparison is also established with other current control, allowing to confirm the precise application of the SMC strategy.This work has been supported by FCT – Fundação para a Ciência e Tecnologia within the Project Scope: UID/CEC/00319/2019. This work has been supported by FCT Project newERA4GRIDs PTDC/EEI-EEE/30283/2017, and by the FCT Project DAIPESEV PTDC/EEI-EEE/30382/2017. Tiago Sousa is supported by the doctoral scholarship SFRH/BD/134353/2017 granted by FCT

A novel multilevel converter for on-grid interface of renewable energy sources in smart grids

The on-grid interface of renewable energy sources involves a dc-ac converter for controlling the injected current. In this perspective, a novel topology of grid-tied converter is proposed, assuming as main feature the produced multilevel voltages (five-levels). The proposed grid-tied converter is intended for on-grid interfaces, which is controlled for guaranteeing sinusoidal currents for all grid voltage conditions. The dc-side can be linked directly to a dc-to-dc intermediary converter, responsible for interfacing renewable energy sources, as solar photovoltaic or wind power systems. Throughout the paper, a complete examination of the operation principle and the adopted control theory, including current control, as well as hardware project, are comprehensively presented. An accurate computational simulation validation is presented, comprising realistic operating conditions in terms of grid voltage disturbances and operating power. The obtained results prove the advantages of the proposed grid-tied multilevel converter, and establish a comparison with the classical solutions.This work has been supported by FCT – Fundação para a Ciência e Tecnologia within the Project Scope: UID/CEC/00319/2019. This work has been supported by FCT Project newERA4GRIDs PTDC/EEI-EEE/30283/2017, and by the FCT Project DAIPESEV PTDC/EEI-EEE/30382/2017. Tiago Sousa is supported by the doctoral scholarship SFRH/BD/134353/2017 granted by FCT

Modelling anaerobic digestion of sewage sludge: mechanistic models vs machine learning

Anaerobic digestion processes are one of the technologies most used by wastewater treatment plants (WWTPs) to stabilize and decrease the organic content of sludge. This process decreases the costs of disposal while increasing the energetic efficiency of WWTPs. In order to optimize this process, three model approaches were implemented. First, we calibrated and validated the anaerobic digestion model no.1 (ADM1) using data from an anaerobic lab digester treating sewage sludge (Phases I, II, III), and further receiving glycerol pulses (Phases IV, V). Then, to optimize the calibration and parameter estimation, an iterative procedure was applied by minimizing the root mean square error (RMSE). The second approach consisted of applying a machine learning (ML) model to the biogas and methane produced. The results showed that the ADM1 model adjusted well to the experimental results, especially to biogas, methane and pH. The optimization routine was useful to identify the most sensitive parameters, improving model calibration. Overall, the ML approach was more reliable to predict anaerobic reactors performance but did not respond so well to process perturbations (glycerol pulses).This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 and UIDB/00319/2020 units and the PAMWater Project (DSAIPA/Al/0099/2019).info:eu-repo/semantics/publishedVersio

Comparative analysis of power electronics topologies to interface dc homes with the electrical ac power grid

This paper presents a comparative analysis of power electronics topologies that can be used to interface dc homes with a 230 V, 50 Hz ac power grid. Dc homes represent an essential asset for smart grids, since energy storage systems and renewable energy sources, such as photovoltaic solar panels, operate in dc, as well as most of the electrical appliances used in domestic scenario. However, since the power grid operates in ac, it is necessary to convert voltage from ac to dc to properly supply a dc home. This conversion can be accomplished in several ways, with different power conversion stages. In this context, this paper analyzes three different possibilities that can be used to perform the interface between the ac power grid and a dc home: (1) ac-dc converter using a low frequency transformer; (2) ac-dc and dc-dc converters using a high frequency transformer; (3) ac-ac and ac-dc converters using a medium frequency transformer. These three possibilities are compared in terms of efficiency, total power factor and total harmonic distortion of the ac power grid. The results were obtained by means of a simulation model based on the internal parameters of the power semiconductors.This work has been supported by FCT –Fundação para a Ciência e Tecnologia within the Project Scope: UID/CEC/00319/2019. This work has been supported by FCT Project DAIPESEV PTDC/EEI-EEE/30382/2017, and by the FCT ProjectnewERA4GRIDs PTDC/EEI-EEE/30283/2017. Mr. Tiago Sousa is supported by the doctoral scholarship SFRH/BD/134353/2017 granted by FCT

Regulation of cell death induced by acetic acid in yeasts

Acetic acid has long been considered a molecule of great interest in the yeast research field. It is mostly recognized as a by-product of alcoholic fermentation or as a product of the metabolism of acetic and lactic acid bacteria, as well as of lignocellulosic biomass pretreatment. High acetic acid levels are commonly associated with arrested fermentations or with utilization as vinegar in the food industry. Due to its obvious interest to industrial processes, research on the mechanisms underlying the impact of acetic acid in yeast cells has been increasing. In the past twenty years, a plethora of studies have addressed the intricate cascade of molecular events involved in cell death induced by acetic acid, which is now considered a model in the yeast regulated cell death field. As such, understanding how acetic acid modulates cellular functions brought about important knowledge on modulable targets not only in biotechnology but also in biomedicine. Here, we performed a comprehensive literature review to compile information from published studies performed with lethal concentrations of acetic acid, which shed light on regulated cell death mechanisms. We present an historical retrospective of research on this topic, first providing an overview of the cell death process induced by acetic acid, including functional and structural alterations, followed by an in-depth description of its pharmacological and genetic regulation. As the mechanistic understanding of regulated cell death is crucial both to design improved biomedical strategies and to develop more robust and resilient yeast strains for industrial applications, acetic acid-induced cell death remains a fruitful and open field of study. © 2021 Chaves, Rego, Martins, Santos-Pereira, Sousa and Côrte-Real.This work was supported by the "Contrato-Programa" UIDB/04050/2020 funded by national funds through the FCT I.P.info:eu-repo/semantics/publishedVersio

Poly(ionic liquid)-based engineered mixed matrix membranes for CO2/H2 separation

Unformatted preprintPoly(ionic liquid)s (PIL) have emerged as a class of versatile polyelectrolites, that can be used to prepare new materials able to achieve superior performances compared to conventional polymers. The combination of PILs with ionic liquids (ILs) may serve as a suitable matrix for the preparation of membranes for gas separation. In this work, mixed matrix membranes (MMMs) combining a pyrrolidinium-based PIL, an IL and three highly CO2-selective metal organic frameworks (MOFs) were prepared. The different MOFs (MIL-53, Cu3(BTC)2 and ZIF-8) were used as fillers, aiming to maximize the membranes performance towards the purification of syngas. The influence of different MOFs and loadings (0, 10, 20 and 30 wt.%) on the thermal and mechanical stabilities of the membranes and their performance in terms of CO2 permeability and CO2/H2 ideal selectivity was assessed. The compatibility between the materials was confirmed by SEM-EDS and FTIR spectroscopy. The prepared MMMs revealed to be thermally stable within the temperature range of the syngas stream, with a loss of mechanical stability upon the MOF incorporation. The increasing MOF content in the MMMs, resulted in an improvement of both CO2 permeability and CO2/H2 ideal selectivity. Among the three MOFs studied, membranes based on ZIF-8 showed the highest permeabilities (up to 97.2 barrer), while membranes based on MIL-53 showed the highest improvement in selectivity (up to 13.3). Remarkably, all permeation results surpass the upper bound limit for the CO2/H2 separation, showing the membranes potential for the desired gas separation.This work was partially supported by R&D Units UID/Multi/04551/2013 (Green-it), UID/QUI/00100/2013 (CQE), and the Associated Laboratory Research Unit for Green Chemistry, Technologies and Clean Processes, LAQV which is financed by national funds from FCT/MCTES(UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). Ana R. Nabais, Luísa A. Neves and Liliana C. Tomé acknowledge FCT/MCTES for financial support through project PTDC/CTM-POL/2676/2014, FCT Investigator Contract IF/00505/2014 and Post-doctoral research grant SFRH/BDP/101793/2014, respectively. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 745734